1. A genetic map of macadamia based on randomly amplified DNA fingerprinting (RAF) markers.
- Author
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Peace, C.P., Vithanage, V., Turnbull, C.G.N., and Carroll, B.J.
- Subjects
MACADAMIA ,PLANT genetics ,GENETICS ,CROP genetics ,DNA fingerprinting ,GENETIC markers - Abstract
The first genetic linkage map of macadamia (Macadamia integrifolia and M. tetraphylla) is presented. The map is based on 56 F
1 progeny of cultivars `Keauhou' and `A16'. Eighty-four percent of the 382 markers analysed segregated as Mendelian loci. The two-way pseudo-testcross mapping strategy allowed construction of separate parental cultivar maps. Ninety bridging loci enabled merging of these maps to produce a detailed genetic map of macadamia, 1100 cm in length and spanning 70–80% of the genome. The combined map comprised 24 linkage groups with 265 framework markers: 259 markers from randomly amplified DNA fingerprinting (RAF), five random amplified polymorphic DNA (RAPD), and one sequence-tagged microsatellite site (STMS). The RAF marker system unexpectedly revealed 16 codominant markers, one of them a putative microsatellite locus and exhibiting four distinct alleles in the cross. This molecular study is the most comprehensive examination to date of genetic loci of macadamia, and is a major step towards developing marker-assisted selection for this crop. [ABSTRACT FROM AUTHOR]- Published
- 2003
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