Ranganath, Dhananjaya, Mazumdar, Abhishek, Mulukuri, Sirisha, Doonaboina, Raghava, Devarakonda, Murty, and Raghu Prasad, Mailavaram
A novel series of 2-substituted-aminomethyl-8-phenyl-pyrazolo[3,4- d][1,3,4]thiadiazolo[3,2- a]pyrimidin-4(1 H)-ones have been synthesized by treating 2-chloromethyl-8-phenyl-pyrazolo[3,4- d][1,3,4]thiadiazolo[3,2- a]pyrimidin-4(1 H)-one with various nucleophiles. The chloromethyl derivative was prepared by treating 2-amino-3-mercapto pyrazolo[3,4- d]pyrimidine with one carbon donor, chloroacetic acid. The intermediate 2-amino-3-mercapto pyrazolo[3,4- d]pyrimidine was prepared by a novel, eco-friendly route starting from 2-amino-3-carbethoxy-1-phenyl-pyrazole. The target compounds exhibited broad-spectrum antibacterial activity (Kirby Bauer's Method). [ABSTRACT FROM AUTHOR]