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Summary: Since the emergence of Middle East respiratory syndrome coronavirus (MERS‐CoV) in 2012, there have been a number of clusters of human‐to‐human transmission. These cases of human‐to‐human transmission involve close contact and have occurred primarily in healthcare settings, and they are suspected to result from repeated zoonotic introductions. In this study, we sequenced whole MERS‐CoV genomes directly from respiratory samples collected from 23 confirmed MERS cases in the United Arab Emirates (UAE). These samples included cases from three nosocomial and three household clusters. The sequences were analysed for changes and relatedness with regard to the collected epidemiological data and other available MERS‐CoV genomic data. Sequence analysis supports the epidemiological data within the clusters, and further, suggests that these clusters emerged independently. To understand how and when these clusters emerged, respiratory samples were taken from dromedary camels, a known host of MERS‐CoV, in the same geographic regions as the human clusters. Middle East respiratory syndrome coronavirus genomes from six virus‐positive animals were sequenced, and these genomes were nearly identical to those found in human patients from corresponding regions. These data demonstrate a genetic link for each of these clusters to a camel and support the hypothesis that human MERS‐CoV diversity results from multiple zoonotic introductions. [ABSTRACT FROM AUTHOR]

Finding predictors of the response to antidepressant therapy is a major goal of molecular psychiatry. The genes encoding the serotonin (SERT) and dopamine (DAT1) transporters are among the possible candidate genes modulating an individual's antidepressant response. In a naturalistic prospective cohort study with a total of 190 fully assessed patients, improvement of depression symptoms during the 3 weeks following initiation of antidepressant therapy was recorded using the 21-item Hamilton Depression Rating Scale (HDRS). The SLC6A3 3′ UTR 40-bp variable number of tandem repeats (VNTR) and the SLC6A4 5′ 44-bp insertion/deletion polymorphism were analyzed by polymerase chain reaction. There was a significantly smaller number of rapid responders among homozygous carriers of the DAT1 9-repeat allele (9/9) than among heterozygous (9/10) and homozygous (10/10) carriers of the 10-repeat allele (19 versus 37 versus 52%, respectively, P=0.0037). Median decline in HDRS score was 35, 40, and 52% in patients with the 9/9, 9/10, and 10/10 genotypes, respectively (P=0.013). The effect was found in all classes of medications (selective serotonin reuptake inhibitors (SSRIs), tricyclics, mirtazapine, venlafaxine) and statistically significant also within the subgroup of patients having received SSRIs. The serotonin promoter insertion/deletion genotype had no effect in the entire study group, but there was an insignificant trend of better response in the l/l and l/s carriers who received SSRIs or mirtazapine. In conclusion, the dopamine transporter VNTR polymorphism influenced rapid response to antidepressant therapy. Compared with homozygous carriers of the 10-repeat allele, carriers of the 9/10 genotype had an odds ratio (OR) calculated by logistic regression analysis of 1.6 (95% CI 0.8–3.2) and carriers of the 9/9 genotype had an OR of 6.0 (1.5–24.4) for no or poor response. Further studies are required to confirm this clinical association and to elucidate the underlying mechanisms.The Pharmacogenomics Journal (2007) 7, 48–55. doi:10.1038/sj.tpj.6500398; published online 9 May 2006 [ABSTRACT FROM AUTHOR]

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Brassinolide (BR), a naturally-occurring-steroidal lactone from rape (Brassica napus L.) pollen, was compared with auxin for activity in a number of bioassay systems. Responses similar to IAA were elicited by BR in bioassays based upon bean hypocotyl hook opening, elongation of maize mesocotyl, pea epicotyl and azuki bean epicotyl sections, and fresh weight increase in Jerusalem artichoke (2,4-D used) and pea epicotyl sections. The azuki bean and dwarf pea epicotyl bioassays were much more responsive to BR than IAA (at 10 μM). Responses approximately two-fold greater in magnitude were elicited by LAA in the maize mesocotyl, bean hypocotyl hook and Jerusalem artichoke bioassays. Little or no response was elicited by BR (0.01 to μM) in the cress root or decapitated pea-lateral bud bioassays. A powerful synergism between BR and IAA was observed in the azuki bean, pea epicotyl and bean hypocotyl hook bioassays. Although, as previously reported, other steroidal substances are active in some of the bioassay systems tested, none compared with BR in magnitude and diversity of elicited responses. [ABSTRACT FROM AUTHOR]

Studies the isolation and characterization of microsatellie markers in Camelus dromedarius and cross-species amplification in C. bactrianus and Lama pacos with the aim of using them in paternity analysis. Indication of the presence of null alleles in C. bactrianus and Lama Pacos; Findings that suggest a larger effective population size in Lama pacos compared to the United Arab Emirates racing horse Camelus dromedarius.

Examines the South American camelid microsatellite amplification in Camelus dromedarius. Analysis of the microsatellites; Observation of the primer pairs amplified in camels genomic DNA; Characterization of the Southern American camels microsatellites.

Cuttings are only a viable alternative to seedlings as planting stock if the method of propagation does not affect their growth and development adversely. Full-sibling cuttings and seedlings of Eucalyptus globulus were compared under controlled environmental conditions to minimise extraneous sources of variation, and to establish whether changes in growth or development were induced by propagation. On three occasions over a period of eight weeks root-collar diameter, shoot height, leaf and stem weight, shoot/root ratios and root system morphology were measured on cuttings and seedlings. Seedlings were taller thancuttings throughout the experiment, but both plant types had similarheight growth rates. Diameter growth rates were lower in cuttings than seedlings, and there were differences in both height and diameter growth rates between families. Root system configuration differed between the plant types. Seedlings had strongly gravitropic tap-roots, with two types of primary roots from which secondary roots emerged. Cuttings had no tap roots, and the main structural components of their root systems were adventitious roots formed during propagation. Cuttings did not develop further structural roots during the experiment, whereas seedlings continued to develop primary roots. Individual primary roots of cuttings were longer and had larger mid-point diameters than those of seedlings, but the total length of primary roots was greater in seedlings. Seedlings also had a greater number and total length of secondary roots. Shoot/root ratios, calculated from a range of functional measures, were higher in cuttings than seedlings. [ABSTRACT FROM AUTHOR]