1. Evaluation of the effect of sample suspension concentration and viral load on the outcome of the rabies tissue culture infection test.
- Author
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de Souza, Tatiane de Cassia Pardo, de Sousa e Silva, Gilmara, de Oliveira Fahl, Willian, Rodrigues, Adriana Candido, Asano, Karen Miyuki, Scheffer, Karin Corrêa, Mori, Enio, Siena, Amanda, and Iamamoto, Keila
- Subjects
TISSUE culture ,RABIES ,CELL preservation ,VIRAL load ,CELL culture ,INFECTION - Abstract
In this study, we examined various brain suspension concentrations and viral loads in Neuro-2a cell cultures using 20 rabies-positive bovine samples. The reproducibility of results varied: 65% showed consistent outcomes across all concentrations, while 35% disagreed in at least one. Viral titers ranged from less than 25 × 10
1 to 25 × 103.50 TCID50 /mL, with 20% below 25 × 101 TCID50 /mL. Concentrations between 5% and 20% yielded over 90% agreement in positive results, but at 30%, agreement dropped from 85% to 50%. Cell confluence was successfully maintained at 5%, 10%, and 20%, while concentrations of 30% and above led to confluence loss. Low viral loads also negatively impacted reproducibility. These results suggest that sample concentration has a direct influence on preservation of cell confluence and that low viral loads may influence the reproducibility of the rabies tissue culture infection test (RTCIT). [ABSTRACT FROM AUTHOR]- Published
- 2024
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