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SUMMARY: Drought has a severe impact on the quality and yield of cotton. Deciphering the key genes related to drought tolerance is important for understanding the regulation mechanism of drought stress and breeding drought‐tolerant cotton cultivars. Several studies have demonstrated that NAC transcription factors are crucial in the regulation of drought stress, however, the related functional mechanisms are still largely unexplored. Here, we identified that NAC transcription factor GhNAC4 positively regulated drought stress tolerance in cotton. The expression of GhNAC4 was significantly induced by abiotic stress and plant hormones. Silencing of GhNAC4 distinctly impaired the resistance to drought stress and overexpressing GhNAC4 in cotton significantly enhanced the stress tolerance. RNA‐seq analysis revealed that overexpression of GhNAC4 enriched the expression of genes associated with the biosynthesis of secondary cell walls and ribosomal proteins. We confirmed that GhNAC4 positively activated the expressions of GhNST1, a master regulator reported previously in secondary cell wall formation, and two ribosomal protein‐encoding genes GhRPL12 and GhRPL18p, by directly binding to their promoter regions. Overexpression of GhNAC4 promoted the expression of downstream genes associated with the secondary wall biosynthesis, resulting in enhancing secondary wall deposition in the roots, and silencing of GhRPL12 and GhRPL18p significantly impaired the resistance to drought stress. Taken together, our study reveals a novel pathway mediated by GhNAC4 that promotes secondary cell wall biosynthesis to strengthen secondary wall development and regulates the expression of ribosomal protein‐encoding genes to maintain translation stability, which ultimately enhances drought tolerance in cotton. Significance Statement: Drought is a big threat to cotton production worldwide. This study identifies that GhNAC4, a member of the NAC family, acts upstream of GhNST1, GhRPL12, and GhRPL18p, modulates secondary cell wall biosynthesis and expression of ribosomal protein genes, and enhances plant drought tolerance in cotton. [ABSTRACT FROM AUTHOR]

Mine earthquakes are serious disasters in coal mines, especially in extremely thick hard strata. This study investigates the occurrence mechanism of fracture-type mine earthquakes in thick hard strata. Hydraulic fracturing by ground vertical well was used for shock absorption. Dongtan coal mine was taken as a case study. Field investigation, theoretical analysis, industrial tests, and field monitoring were used for revealing the mechanism. First, the mechanical model of extremely thick, hard strata under horizontal concentrated stress was established. The fracture step equation and energy release equation of extremely thick hard rock were derived by semi-inverse solution and variational method. Then, the mechanical model of extremely thick hard rock after hydraulic fracturing by ground vertical well was established. The relationship between the spacing of the ground vertical well and the maximum magnitude of mine earthquakes was deduced. The fracturing well in the 6306 working face was designed for controlling the maximum mine earthquake magnitude. Results show that the increases in the breaking distance of the thick hard rock layer led to an increase in the released energy during the fracture, and an enhancement of the magnitude of the mine earthquake. By applying hydraulic fracturing technology using the ground vertical shaft, the occurrence frequency and total energy of mine earthquakes above 1.5 ML in the 6306 working face decreased by 54.55% and 81.22% than that in 6304 working face, and reduced by 70% and 84.98% than that in 6305 working face. Hydraulic fracturing technology by ground vertical well can significantly reduce the frequency of fracture-type and the total energy of mine earthquakes in extremely thick and hard strata. However, it can not prevent and control the occurrence of back-transition mine earthquakes and slip-type mine earthquakes. The obtained results can provide a basis for the fracture-type mine earthquake mechanism and fracturing shock absorption technology in coal mines with super-thick hard strata. [ABSTRACT FROM AUTHOR]

With the increase in mining depth and the deterioration of mining conditions, thick and hard overburden movement frequently induces mine earthquakes and rock bursts. Some mines are expected to prevent and control super thick hard rock mine earthquakes through vertical ground well water fracturing technology. However, the dynamic underground disaster appears more intense. Taking the '11.30' mine earthquake in a mine in Shandong Province as the engineering background, the dynamic disaster mechanism of an extraordinarily thick and hard roof induced by hydraulic fracturing of vertical wells on the ground was studied utilizing field investigation, accident case analysis, similar material simulation test, and theoretical analysis. The main conclusions are as follows: (1) After hydraulic fracturing vertical wells on the ground, the movement mode of thick and hard roofs changed from layer-by-layer to overall sliding movement; (2) The influence range of the advanced abutment pressure of the working face is reduced by the hydraulic fracturing of the vertical shaft, and the peak value of the advanced abutment pressure increases. Furthermore, the advanced abutment pressure's peak is far from the coal wall; (3) The hydraulic fracturing technology of cross-arranged vertical surface deep and shallow wells and the hydraulic fracturing technology of cross-perforated surface multi-branch horizontal wells are proposed to avoid the dynamic disaster of overall sliding movement of an extremely thick hard roof induced by surface hydraulic fracturing. Therefore, these research results provide significance for preventing and controlling mine earthquakes and rock bursts in super thick hard roof mines. [ABSTRACT FROM AUTHOR]

SUMMARY: Global climate changes cause an increase of abiotic and biotic stresses that tremendously threaten the world's crop security. However, studies on broad‐spectrum response pathways involved in biotic and abiotic stresses are relatively rare. Here, by comparing the time‐dependent transcriptional changes and co‐expression analysis of cotton (Gossypium hirsutum) root tissues under abiotic and biotic stress conditions, we discovered the common stress‐responsive genes and stress metabolism pathways under different stresses, which included the circadian rhythm, thiamine and galactose metabolism, carotenoid, phenylpropanoid, flavonoid, and zeatin biosynthesis, and the mitogen‐activated protein kinase signaling pathway. We found that thiamine metabolism was an important intersection between abiotic and biotic stresses; the key thiamine synthesis genes, GhTHIC and GhTHI1, were highly induced at the early stage of stresses. We confirmed that thiamine was crucial and necessary for cotton growth and development, and its deficiency could be recovered by exogenous thiamine supplement. Furthermore, we revealed that exogenous thiamine enhanced stress tolerance in cotton via increasing calcium signal transduction and activating downstream stress‐responsive genes. Overall, our studies demonstrated that thiamine played a crucial role in the tradeoff between plant health and stress resistance. The thiamine deficiency caused by stresses could transiently induce upregulation of thiamine biosynthetic genes in vivo, while it could be totally salvaged by exogenous thiamine application, which could significantly improve cotton broad‐spectrum stress tolerance and enhance plant growth and development. Significance Statement: Thiamine plays a crucial role in regulating plant health and stress resistance. In cotton (Gossypium hirsutum), exogenous thiamine could maintain normal functioning of plants and improve broad‐spectrum stress tolerance by modulating thiamine metabolism and activating calcium signal transduction and stress response pathways. This study has practical significance in enhancing broad‐spectrum stress tolerance of crop plants in changing environments. [ABSTRACT FROM AUTHOR]

Cotton fiber is the most important natural textile material in the world. Identification and functional characterization of genes regulating fiber development are fundamental for improving fiber quality and yield. However, stable cotton transformation is time-consuming, low in efficiency, and technically complex. Moreover, heterologous systems, such as Arabidopsis and tobacco, did not always work to elucidate the function of cotton fiber specifically expressed genes or their promoters. For these reasons, constructing a rapid transformation system using cotton fibers is necessary to study fiber's specifically expressed genes. In this study, we developed an easy and rapid Agrobacterium -mediated method for the transient transformation of genes and promoters in cotton fibers. First, we found that exogenous genes could be expressed in cotton fibers via using β-glucuronidase (GUS) and green fluorescence protein (GFP) as reporters. Second, parameters affecting transformation efficiency, including LBA4404 Agrobacterium strain, 3 h infection time, and 2-day incubation time, were determined. Third, four different cotton genes that are specifically expressed in fibers were transiently transformed in cotton fibers, and the transcripts of these genes were detected ten to thousand times increase over the control. Fourth, GUS staining and activity analysis demonstrated that the activity profiles of GhMYB212 and GhFSN1 promoters in transformed fibers are similar to their native activity in developmental fibers. Furthermore, the transient transformation method was confirmed to be suitable for subcellular localization studies. In summary, the presented Agrobacterium -mediated transient transformation method is a fast, simple, and effective system for promoter characterization and protein expression in cotton fibers. [ABSTRACT FROM AUTHOR]

Inhibition of phosphodiesterase 2 and 4 (PDE2A and PDE4) increases the intracellular cAMP and/or cGMP levels, which may prevent Amyloid β 42 oligomers (Aβ) related cognitive impairment and dementias. Baicalein, one of natural flavones found in the root of Scutellaria baicalensis Georgi, has a wide range of pharmacological activities including antioxidant and anti-inflammatory effects. However, no studies suggest whether baicalein mediated anti-Alzheimer's disease (AD) events involve PDEs subtypes-mediated neuroprotective pathways. The present study examined whether memory enhancing effects of baicalein on Aβ- induced cognitive impairment are related to regulating neuroplasticity via PDE2 and PDE4 subtypes dependent cAMP/cGMP neuroprotective pathway. The results suggested that microinjected of Aβ into CA1 of hippocampus induced cognitive and memory impairment in mice, as evidenced by decreased recognition index in the novel object recognition (NOR) task, impaired memory acquisition, retention and retrieval in the Morris water maze (MWM) and shuttle box tests. These effects were reversed by treatment with baicalein for 14 days. Moreover, Aβ-induced neuronal atrophy and decreased expression of two synaptic proteins, synaptophysin and PSD 95, were prevented by baicalein. The increased expression of PDE2A and PDE4 subtypes (PDE4A, PDE4B and PDE4D), and decreased levels of cAMP/cGMP, pCREB/CREB and BDNF induced by Aβ were also blocked by chronic treatment of baicalein for 14 days. These findings suggest that baicalein's reversal of Aβ-induced memory and cognitive disorder may involve the regulation of neuronal remodeling via regulation of PDE2/PDE4 subtypes related cAMP/cGMP -pCREB-BDNF pathway. [ABSTRACT FROM AUTHOR]

With the development of faults in many coalfields, many large faults will form a relatively independent area, forming regional tectonic stress concentration. Under the influence of mining, it is easy to induce fault activation, produce mine tremor, and then induce rock burst. Through field investigation, theoretical analysis, numerical simulation, and engineering verification, the overburden movement model of No. 3504 working face square and fault activation in Liangbaosi Coal Mine was established. The stress variation and energy release law of working face advance and fault area were analyzed, and the mechanism of rock burst under the coupling action of working face square and regional tectonic stress was revealed. The results show that the regional stress adjustment and fault activation are caused by the large-scale overall movement of overburden during the working face square, and there is a peak value of elastic energy release during the fault activation, which is easy to produce large energy mine earthquake. The energy level of the daily maximum energy event is higher than that of the initial mining stage in the square period, and the location of on-site large energy microseismic event is basically consistent with the predicted fault strike. The study provides a theoretical basis for the prevention and control of rock burst during the working face square under the condition of regional tectonic stress. [ABSTRACT FROM AUTHOR]

Fucosylation, one of the key posttranslational modifications, plays an important role in plants. It is involved in the development, signal transduction, reproduction, and disease resistance. α1,3-/4-Fucosyltransferase is responsible for transferring L-fucose from GDP-L-fucose to the N-glycan to exert fucosylational functions. However, the roles of the fucosyltransferase gene in cotton remain unknown. This study provided a comprehensive investigation of its possible functions. A genome-wide analysis identified four, four, eight, and eight FucT genes presented in the four sequenced cotton species, diploid Gossypium raimondii, G. arboreum, tetraploid G. hirsutum acc. TM-1, and G. barbadense cv. H7124, respectively. These FucTs were classified into two groups, with FucT4 homologs alone as a group. We isolated FucT4 in TM-1 and H7124, and named it GhFucT4 and GbFucT4, respectively. Quantitative RT-PCR and transcriptome data demonstrated that GhFucT4 had the highest expression levels in fibers among all GhFucT genes. Association studies and QTL co-localization supported the possible involvement of GhFucT4 in cotton fiber development. GhFucT4 and GbFucT4 shared high sequence identities, and FucT4 had higher expression in H7124 fiber tissues compared with TM-1. Furthermore, ectopic expression of FucT4 in transgenic Arabidopsis promoted root cell elongation, upregulated expression of genes related to cell wall loosening, and led to longer primary root. These results collectively indicate that FucT4 plays an important role in promoting cell elongation and modulating fiber development, which could be utilized to improve fiber quality traits in cotton breeding. [ABSTRACT FROM AUTHOR]

Cellulose is a major component of plant cell walls and is necessary for plant morphogenesis and biomass. COBL (COBRA-Like) proteins have been shown to be key regulators in the orientation of cell expansion and cellulose crystallinity status. To clarify the role of a cotton COBL gene, GhCOBL9A, we conducted the ectopic expression and functional analysis in Arabidopsis. Previous study showed that GhCOBL9A was preferentially expressed during secondary cell wall biosynthesis in cotton fibers, and showed a significant co-expression pattern with cellulose synthase genes. Here, we detected that overexpression of GhCOBL9A induced the up-regulation of genes related to cellulose synthesis and enhanced the cellulose deposition. As a result, GhCOBL9A transgenic plants displayed increased hypocotyl and root lengths in early development, and cell wall thickening at the SCW stage. Notably, overexpression of GhCOBL9A led to an erect, robust-stature phenotype and brought higher biomass in mature plants. In addition, overexpression of GhCOBL9A in Arabidopsis AtCOBL4 mutants, a paralogous gene of GhCOBL9A, also led to a stronger growth potential, but the Atcobl4 mutant phenotype could not be rescued, implying the functional divergence of GhCOBL9A and AtCOBL4 paralogs. Taken together, these results suggest that overexpression of GhCOBL9A contributes to plant cell elongation and thickening, and increased biomass, which provides references for further utilizing GhCOBL9A to improve yield and quality traits in cotton and other species. [ABSTRACT FROM AUTHOR]

In recent years, microorganisms such as algae have been found on composite and RTV coated insulators in humid environment in southwest and southern China. Algae growth poses a potential threat to the outer insulation of electrical power system because of the special features of biological foul. Insulator samples with algae growth were obtained from transmission towers of 500 kV Tianguang DC transmission line and the influence of algae growth on the external insulation performance was studied in this paper. A measurement circuit was used to measure the partial surface conductivity of algae and non-algae dots on insulator surfaces. Water spray classification method was used to test the hydrophobicity of algae and non-algae parts of the insulators. An image processing method was proposed to discern algae on the insulator surfaces and calculate the algae coverage rate. The withstand capability of insulator were tested at working voltage and 1.2 times working voltage. The research results showed that the presence of algae increased the local salt distribution and reduced the hydrophobicity of insulator surfaces. The results also indicated that impacts of algae contanmination to withstand capability of insulators were limited when the algae coverage rate was less than 20%. [ABSTRACT FROM AUTHOR]

Cotton is an economically important crop grown for natural fiber and seed oil production. Cottonseed oil ranks third after soybean oil and colza oil in terms of edible oilseed tonnage worldwide. The fatty acid composition of cottonseed oil determines its industrial application and nutritional values. However, little progress has been made in understanding cottonseed oil biogenesis. Stearoyl-acyl carrier protein desaturase (SAD), the only known enzyme to convert saturated fatty acids into unsaturated fatty acids in plants, plays key roles in determining the fatty acid composition of cottonseed oil. In this study, we identified 9, 9, 18 and 19 SAD genes in the genomes of four sequenced cotton species: diploid Gossypium raimondii (D), G. arboreum (A), tetraploid G. hirsutum acc. TM-1 (AD) and G. barbadense cv. Xinhai21 (AD), respectively. Bioinformatic and phylogenetic analyses revealed that cotton SADs can be classified into two classes. Expression patterns showed developmental and spatial regulation of SADs in cotton. GhSAD2 and GhSAD4 were preferentially expressed in developing ovules 20-35 days post-anthesis, and significantly different expression patterns were found between high-oil and low-oil cotton cultivars, implying these two genes could be involved in cottonseed oil biogenesis. Association analysis further confirmed that GhSAD4- At expression was closely related to the oleic acid (O) content, linoleic acid (L) content and O/L value in cottonseed, implying GhSAD4 plays an important role in cottonseed oil composition. This study brings new perspectives for integrated genome-wide identification of SADs in cotton and provides references for the genetic improvement of cottonseed oil. [ABSTRACT FROM AUTHOR]

Verticillium dahliae infection leads to Verticillium wilt in cotton and other dicotyledon crops. To reduce the loss of economic crops, more attention has been focused on the key genes involved in pathogenicity of this soil-borne plant fungal pathogen. Sho1 encodes a conserved tetraspan transmembrane protein which is a key element of the two upstream branches of the HOG- MAPK pathway in fungi. Sho1 is required for full virulence in a wide variety of pathogenic fungi. In this study, sho1 mutant in V. dahliae (designated ΔVdsho1) was generated by Agrobacterium tumefaciens-mediated transformation. ΔVdsho1 strain was highly sensitive to menadione (at concentration of 120 μ m) and hydrogen peroxide (at concentration of 250 μ m), displayed delayed spore germination and reduced spore production compared with the wild type and the complemented strains. During infection of host cotton plants, ΔVdsho1 exhibited impaired ability of root attachment and invasive growth. Results from the present work suggest that VdSho1 controls external sensing, virulence and multiple growth-related traits in V. dahliae and might serve as a potential target for control of Verticillium wilt. [ABSTRACT FROM AUTHOR]

Members of the CrRLK1L family, a subgroup of the receptor-like kinase (RLK) gene family, are thought to act as sensors for the integrity of the cell wall and regulators of polar elongation. To better understand the various functions in fiber development, we conducted genome-wide identification and characterization analyses of CrRLK1L family in cotton. Here 44, 40, and 79 CrRLK1L genes were identified from three cotton species: diploid G. raimondii (D), diploid G. arboreum (A), and tetraploid G. hirsutum TM-1 (AD), respectively. The 44 CrRLK1Ls in G. raimondii were anchored to the 12 chromosomes unevenly and were classified into six groups (I-VI), with group II and group IV being further divided into two subgroups (groups IIa and IIb, and IVa and IVb, respectively). These CrRLK1Ls displayed a highly regular pattern of developmental and spatial regulation in cotton. Using the transcriptome data of five chromosomal segment introgression lines (CSILs) and the physical integration of CrRLK1Ls with the quantitative trait loci (QTLs) related to fiber quality traits, we revealed that six CrRLK1L genes were highly associated with fiber development. This study brings new insights into the integrated genome-wide identification of CrRLK1Ls in cotton and provides references for the genetic improvement of cotton fiber. [ABSTRACT FROM AUTHOR]

COBRA-Like (COBL) genes, which encode a plant-specific glycosylphosphatidylinositol (GPI) anchored protein, have been proven to be key regulators in the orientation of cell expansion and cellulose crystallinity status. Genome-wide analysis has been performed in A. thaliana, O. sativa, Z. mays and S. lycopersicum, but little in Gossypium. Here we identified 19, 18 and 33 candidate COBL genes from three sequenced cotton species, diploid cotton G. raimondii, G. arboreum and tetraploid cotton G. hirsutum acc. TM-1, respectively. These COBL members were anchored onto 10 chromosomes in G. raimondii and could be divided into two subgroups. Expression patterns of COBL genes showed highly developmental and spatial regulation in G. hirsutum acc. TM-1. Of them, GhCOBL9 and GhCOBL13 were preferentially expressed at the secondary cell wall stage of fiber development and had significantly co-upregulated expression with cellulose synthase genes GhCESA4, GhCESA7 and GhCESA8. Besides, GhCOBL9 Dt and GhCOBL13 Dt were co-localized with previously reported cotton fiber quality quantitative trait loci (QTLs) and the favorable allele types of GhCOBL9 Dt had significantly positive correlations with fiber quality traits, indicating that these two genes might play an important role in fiber development. [ABSTRACT FROM AUTHOR]

In deep mines, a reasonable design of the widths of isolated coal pillars (ICPs) is critically important, particularly for hard-strata mines. This is because the frequent occurrence of mine seismicity (MS) and rockburst in deep mines often arises from the inappropriate widths of the remnant ICPs. To address this problem, this paper takes the ICP of Yingpanhao Coal Mine in Inner Mongolia in China as the engineering case study and then presents a mechanical model to illuminate the occurrence mechanism of MS induced by the mining on both sides of ICPs. The results indicate that, after the mining on both sides of ICPs, the ICPs will produce a vertical compressive deformation, and the overlying high main key stratum (MKS) will experience a flexure deformation. When the limited deflection of MKS is less than the compression of ICPs, the MKS will be fractured, and the released energy may lead to MS. Based on the mechanism model, a design criterion is proposed for ICP widths; this criterion can effectively reduce the risk of the induced rockburst and MS. Then the occurrence mechanism of MS and the design basis for ICP width are verified by numerical simulation and field microseismic monitoring. The results in this paper may be used as a theoretical guidance for rational ICP design in deep mines and may help mitigate the risk of rockburst and MS from early mining stages. [ABSTRACT FROM AUTHOR]

Key message: Cellular comparisons on three legume species indicate oil body size is factor in mediating oil content. Key transcription factors and oleosins determining seed oil content were identified in somatic embryos of Medicago. Legume seeds provide a significant oil alternative to meat fat needs. Increasing demand for oil nutrition in the context of sustainable crop production has stimulated the exploration of legume seed oil storage regulation. This study investigated the cellular characteristics of seed oil storage using three legume species i.e. Medicago truncatula, Glycine max and Pongamia pinnata representing different oil/protein ratios, and then examined in vitro approaches for assessing strategies in enhancing seed oil storage. A greater range of oil body sizes was in higher oil/protein content species, with highest species having the largest oil bodies; and the smallest oil body size being relatively similar across species, suggesting that the arrangement of oil body size may be factor in mediating oil content. The expression of four key transcription factors i.e. LEC1, L1L, FUS3 and ABI3, and four oleosin genes in determining seed oil content was compared in vivo and in vitro using somatic embryos in Medicago, along with cellular evidence of oil bodies in somatic embryos, indicating that somatic embryos may be suitable models for rapid assessment of seed oil enhancement. This study revealed the cellular characteristics for legume seed oil storage with different nutritional compositions, and identified the associated molecular basis for boosting seed oil storage via regulating oil body size. In addition, somatic embryogenesis may be an effective system for examining oil production by modifying the expression of candidate genes prior to in vivo testing. [ABSTRACT FROM AUTHOR]

This article has been retracted. [ABSTRACT FROM AUTHOR]