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Aim: The current study was conducted to assess the effects of various surface treatments on the implant surface. Materials and Methods: A total of 45 dental implants measuring 16 mm in length and 5 mm in diameter were used, along with the appropriate abutments. Depending on the surface treatment used, the samples were randomly split into three groups, with 15 implants in each group: Group 1: Control, Group 2: UV light-treated, Group 3: Sandblasted and acid-etched (SLA)-treated. After surface treatment, a scanning electron microscope (SEM) was used to assess the test samples' surface properties. All sample images were captured using a 3000× magnification. After all three groups' surfaces were treated, the surface roughness was measured using a digital optical profilometer with a stylus speed of 0.5 mm/s that was connected to computer software. Results: The maximum surface roughness was found in the group treated with SLA (0.714 ± 0.12), followed by the group treated with UV light (0.692 ± 0.09) and the control group (0.516 ± 0.12). There was a significant difference found between different surface treatment methods. Conclusion: The present study concluded that the group that received the SLA treatment had the highest surface roughness when compared to the UV light and control groups. [ABSTRACT FROM AUTHOR]

Objective: Use next-generation sequencing (NGS) technology to improve our diagnostic yield in patients with suspected genetic disorders in the Asian setting.Design: A diagnostic study conducted between 2014 and 2019 (and ongoing) under the Singapore Undiagnosed Disease Program. Date of last analysis was 1 July 2019.Setting: Inpatient and outpatient genetics service at two large academic centres in Singapore.Patients: Inclusion criteria: patients suspected of genetic disorders, based on abnormal antenatal ultrasound, multiple congenital anomalies and developmental delay.Exclusion Criteria: patients with known genetic disorders, either after clinical assessment or investigations (such as karyotype or chromosomal microarray).Interventions: Use of NGS technology-whole exome sequencing (WES) or whole genome sequencing (WGS).Main Outcome Measures: (1) Diagnostic yield by sequencing type, (2) diagnostic yield by phenotypical categories, (3) reduction in time to diagnosis and (4) change in clinical outcomes and management.Results: We demonstrate a 37.8% diagnostic yield for WES (n=172) and a 33.3% yield for WGS (n=24). The yield was higher when sequencing was conducted on trios (40.2%), as well as for certain phenotypes (neuromuscular, 54%, and skeletal dysplasia, 50%). In addition to aiding genetic counselling in 100% of the families, a positive result led to a change in treatment in 27% of patients.Conclusion: Genomic sequencing is an effective method for diagnosing rare disease or previous 'undiagnosed' disease. The clinical utility of WES/WGS is seen in the shortened time to diagnosis and the discovery of novel variants. Additionally, reaching a diagnosis significantly impacts families and leads to alteration in management of these patients. [ABSTRACT FROM AUTHOR]

Aim: The aim of the study was to evaluate the patients' expectation and satisfaction with complete dentures before and after the treatment concerning retention, mastication, phonetics, esthetics, and comfort among the first-time denture wearers and already denture wearers and to find the correlation between duration of denture-wearing experience and satisfaction. Settings and Design: Cross sectional survey. Materials and Methods: A questionnaire was given to the patient before the onset of the treatment, and ratings were given by the patient for expectation regarding retention, mastication, phonetics, esthetics, and comfort. Patient-related variables regarding previous denture experience and duration of wearing were also recorded. After the treatment was completed, the patient was asked to complete the same questionnaire to assess the satisfaction. Statistical Analysis Used: Descriptive statistics, frequency and percentage analysis, Wilcoxon signed-rank test, Mann-Whitney U-test. Results: Expectation and satisfaction were met for all the variables except mastication (P = 0.004) for first-time wearers and except mastication (P = 0.001) and comfort (P = 0.007) among existing denture wearers. However, no significant correlation was elicited between patient expectation and satisfaction in both these groups as the overall mean satisfaction percentage was similar. A clinical significance of P = 0.037 was seen with respect to the duration of denture-wearing experience among existing wearers. Regression model analysis showed a decreased satisfaction with increased duration of wearing (r = 0.396). Conclusions: Satisfaction and expectation were found to be almost the same irrespective of whether they are first-time wearers or existing wearers. With the increase in the duration of denture-wearing experience, the satisfaction of the patients decreased. [ABSTRACT FROM AUTHOR]

Background: Dyslipidemia is one of the most frequently implicated risk factors for development of atherosclerosis. This study evaluated the efficacy of amla (Emblica officinalis) extract (composed of polyphenols, triterpenoids, oils etc. as found in the fresh wild amla fruit) in patients with dyslipidemia. Methods: A total of 98 dyslipidemic patients were enrolled and divided into amla and placebo groups. Amla extract (500 mg) or a matching placebo capsule was administered twice daily for 12 weeks to the respective group of patients. The patients were followed up for 12 weeks and efficacy of study medication was assessed by analyzing lipid profile. Other parameters evaluated were apolipoprotein B (Apo B), apolipoprotein A1 (Apo A1), Coenzyme Q10 (CoQ10), high-sensitive C-reactive protein (hsCRP), fasting blood sugar (FBS), homocysteine and thyroid stimulating hormone (TSH). Results: In 12 weeks, the major lipids such as total cholesterol (TC) (p = 0.0003), triglyceride (TG) (p = 0.0003), low density lipoprotein cholesterol (LDL-C) (p = 0.0064) and very low density lipoprotein cholesterol (VLDL-C) (p = 0.0001) were significantly lower in amla group as compared to placebo group. Additionally, a 39% reduction in atherogenic index of the plasma (AIP) (p = 0.0177) was also noted in amla group. The ratio of Apo B to Apo A1 was reduced more (p = 0.0866) in the amla group as compared to the placebo. There was no significant change in CoQ10 level of amla (p = 0.2942) or placebo groups (p = 0.6744). Although there was a general trend of FBS reduction, the numbers of participants who may be classified as pre-diabetes and diabetes groups (FBS > 100 mg/dl) in the amla group were only 8. These results show that the amla extract used in the study is potentially a hypoglycaemic as well. However, this needs reconfirmation in a larger study. Conclusions: The Amla extract has shown significant potential in reducing TC and TG levels as well as lipid ratios, AIP and apoB/apo A-I in dyslipidemic persons and thus has scope to treat general as well as diabetic dyslipidemia. A single agent to reduce cholesterol as well as TG is rare. Cholesterol reduction is achieved without concomitant reduction of Co Q10, in contrast to what is observed with statins. Trial registration: Registered with Clinical Trials Registry- India at www.ctri.nic.in (Registration number: CTRI/2015/04/005682) on 8 April 2015 (retrospectively registered). [ABSTRACT FROM AUTHOR]

Small amphiphilic molecules, also known as hydrotropes, are too small to form micelles in aqueous solutions. However, aqueous solutions of nonionic hydrotropes show the presence of a dynamic, loose, non-covalent clustering in the water-rich region. This clustering can be viewed as “micelle-like structural fluctuations”. Although these fluctuations are short ranged (∼1 nm) and short lived (10 ps–50 ps), they may lead to thermodynamic anomalies. In addition, many experiments on aqueous solutions of hydrotropes show the occasional presence of mesoscale (∼100 nm) inhomogeneities. We have combined results obtained from molecular dynamics simulations, small-angle neutron scattering, and dynamic light-scattering experiments carried out on tertiary butyl alcohol (hydrotrope)–water solutions and on tertiary butyl alcohol–water–cyclohexane (hydrophobe) solutions to elucidate the nature and structure of these inhomogeneities. We have shown that stable mesoscale inhomogeneities occur in aqueous solutions of nonionic hydrotropes only when the solution contains a third, more hydrophobic, component. Moreover, these inhomogeneities exist in ternary systems only in the concentration range where structural fluctuations and thermodynamic anomalies are observed in the binary water–hydrotrope solutions. Addition of a hydrophobe seems to stabilize the water–hydrotrope structural fluctuations, and leads to the formation of larger (mesoscopic) droplets. The structure of these mesoscopic droplets is such that they have a hydrophobe-rich core, surrounded by a hydrogen-bonded shell of water and hydrotrope molecules. These droplets can be extremely long-lived, being stable for over a year. We refer to the phenomenon of formation of mesoscopic droplets in aqueous solutions of nonionic hydrotropes containing hydrophobes, as mesoscale solubilization. This phenomenon may represent a ubiquitous feature of nonionic hydrotropes that exhibit clustering in water, and may have important practical applications in areas, such as drug delivery, where the replacement of traditional surfactants may be necessary. [ABSTRACT FROM AUTHOR]

Aim: The aim of this study was to examine the impact of thermocycling on the flexural strength and development of surface flaws on the glazed surface of porcelain laminate veneer restorations with and without resin luting cement. Materials and Methods: 80 Vitadur alpha dentin porcelain discs (10 mm diameter, 0.9 mm thickness) were glazed on one side and divided into two groups: A (porcelain laminate veneer only without resin luting cement) and B (porcelain laminate veneer luted with resin cement), each containing 40 discs. The discs in groups A and B were then thermocycled at different temperatures and were subjected to SEM analysis to evaluate the effect of thermocycling on crack propagation. Mean flexural strength was determined by using the ball-on-ring test. Student's t-test was used to find out the difference between strength values of the thermocycled porcelain discs and discs luted with resin cement. Results: SEM analysis revealed crack propagation in the subgroups subjected to extremes of temperature, i.e., 4 ± 1°C, 37 ± 1°C and 4 ± 1°C, 65 ± 1°C in the porcelain laminate veneers luted with resin cement. Flexural strength analysis revealed superior flexural strength for porcelain laminate veneers: 88.58 ± 6.94 Mpa when compared to porcelain laminate veneers luted with resin cement: 8.42 ± 2.60 MPa. Results were tabulated and statistically analyzed using Student's t-test. Conclusion: Laminate veneer specimens exhibited greater flexural strength than those which were luted with resin cements. Laminate veneer specimens luted with resin cement and subjected to extremes of temperature, 4 ± 1°C and 37 ± 1°C and 4 ± 1°C and 65 ± 1°C, showed a marked decrease in flexural strength. After thermocycling at extremes of temperature, laminate veneer specimens luted with resin cement showed crack propagation. Fit of laminate veneers cannot / should not be compensated by the thickness of luting agent. [ABSTRACT FROM AUTHOR]

Antigens and mitogens have the innate ability to trigger cell proliferation and apoptosis thus exhibiting a dual-signal phenomenon. This dual-signal hypothesis was tested with mycobacterial antigens (PPD and heat killed Mycobacterium tuberculosis – MTB) in tuberculous pleuritis patients where the immune response is protective and compartmentalized. We compared and correlated the cell-cycle analysis and antigen-induced apoptosis in normal and patients’ peripheral blood mononuclear cells (PBMCs) and patients’ pleural fluid mononuclear cells (PFMCs).In cell-cycle analysis, PFMCs showed good mitotic response with PPD and MTB antigens where 10% and 7% of resting cells entered the S and G2/M phases of cell cycle, respectively. This antigen-induced proliferation of PFMCs correlated well with the lymphocyte transformation test (LTT) results. On the other hand, PFMCs also showed 21% of spontaneous apoptosis, which further increased to 43%, by induction with known apoptotic agent like Dexamethasone (DEX) and the mycobacterial antigens PPD and MTB. Further we demonstrated by anti-CD3 induction experiments that prior activation of cells is prerequisite for them to undergo apoptosis. Our results showed that PPD and MTB antigens induced both cell proliferation and apoptosis in PFMCs, which were pre-sensitized to mycobacterial antigens in vivo. Thus the dual-signal phenomenon was operative against these antigens in tuberculous pleuritis. We also demonstrated that the activated cells are more predisposed to apoptosis. [Copyright &y& Elsevier]

Telomeres vary greatly in size among plants and, in most higher plants, consist of a long array of 5′-TTTAGGG-3′/3′-AAATCCC-5′ (TTTAGGG) repeats. Recently, telomeric DNA in human, mouse, oxytricha, and trypanosome chromosomes have been found arranged into loops (t-loops), proposed to sequester the telomere from unwanted repair events and prevent activation of DNA damage checkpoints. We have asked whether t-loops exist in the higher order plant Pisum sativum (garden pea). DNA was isolated from the shoots and root tips of germinating seeds. Analysis of the telomeric restriction fragments showed that DNA hybridizing to a (TTTAGGG) n probe migrated as a smear centering around 25 kb, and direct sequencing verified the repeat to be (TTTAGGG) n. Total DNA in isolated nuclei was photo-cross-linked, and the telomeric restriction fragments were purified by gel filtration. Electron microscopic (EM) analysis revealed DNA molecules arranged as t-loops with a size distribution consistent with that seen by gel electrophoresis. Some molecules had loops as large as 75 kb. These results show that the arrangement of telomeric DNA into loops occurs in higher plants. [ABSTRACT FROM AUTHOR]

Proposes a model for the mechanism of topology simplification by type II DNA topoisomerases. Qualitative analysis of the model by computer simulations; Description of the mechanism used by type II topoisomerases to actively disentangle DNA.