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BACKGROUND: Grapevine red blotch virus (GRBV) is the causal agent of grapevine red blotch disease and is known to delay grape ripening. However, grape cell‐wall modifications during GRBV infection are largely unknown, even though the cell wall plays a large role in pathogenicity, viral interactions with host plants, and phenolic extractability during winemaking. Understanding the impact of GRBV infection on cell‐wall metabolism is important for the development of potential mitigations strategies. In this study, high‐throughput transcriptome sequencing was conducted on Vitis vinifera L. 'Merlot' grapes during ripening. The cell‐wall composition, phenolic content, and phenolic extractability at two different commercial harvest points were also determined. RESULTS: Log fold changes indicated a strong induction in diseased grapes at harvest of several transcripts involved in cell‐wall solubilization and degradation. However, these observations did not translate to changes in cell‐wall composition at either harvest point in diseased grapes, potentially suggesting post‐transcriptional regulation. Moderate induction of pectin methylesterase inhibitor transcripts and transcripts associated with pathogenesis‐related proteins coincided with increases in pectin and soluble proteins in cell walls of diseased grapes at harvest. Both pectin and pathogenesis‐related proteins are known to retain phenolic compounds during winemaking. CONCLUSION: Our study corroborates this finding when the percentage extractability of flavonols in wines was significantly lower when made from GRBV‐infected fruit. These results suggest GRBV alters the grape cell walls, consequently decreasing phenolic extraction during winemaking. © 2023 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Grapevine red blotch virus (GRBV) is a recently identified virus. Previous research indicates primarily a substantial impact on berry ripening in all varieties studied. The current study analyzed grapes' primary and secondary metabolism across grapevine genotypes and seasons to reveal both conserved and variable impacts to GRBV infection. Vitis vinifera cv. Cabernet Sauvignon (CS) grapevines grafted on two different rootstocks (110R and 420A) were analyzed in 2016 and 2017. Metabolite profiling revealed a considerable impact on amino acid and malate acid levels, volatile aroma compounds derived from the lipoxygenase pathway, and anthocyanins synthesized in the phenylpropanoid pathway. Conserved transcriptional responses to GRBV showed induction of auxin-mediated pathways and photosynthesis with inhibition of transcription and translation processes mainly at harvest. There was an induction of plant-pathogen interactions at pre-veraison, for all genotypes and seasons, except for CS 110R in 2017. Lastly, differential co-expression analysis revealed a transcriptional shift from metabolic synthesis and energy metabolism to transcription and translation processes associated with a virus-induced gene silencing transcript. This plant-derived defense response transcript was only significantly upregulated at veraison for all genotypes and seasons, suggesting a phenological association with disease expression and plant immune responses. [ABSTRACT FROM AUTHOR]

The three-cornered alfalfa hopper, Spissistilus festinus (Say) was shown to transmit Grapevine red blotch virus (GRBV) in a greenhouse study. GRBV is the causal agent of Grapevine Red Blotch Disease, which reduces the quality of wine produced from infected grapes. Due to the general lack of prior concern regarding S. festinus on grapevines, the biology of this species in vineyards has been largely unknown. A 2-yr study with weekly sampling was conducted in a Californian vineyard to increase the knowledge of S. festinus seasonal dynamics and distribution. The overwintering S. festinus adults were first captured in the vineyard before bud break. Detection of late-instar S. festinus nymphs, the first in-field adult generation, and grape anthesis occurred concurrently in 2016 and 2017. Two in-field S. festinus generations were documented by peaks in sweep net sampling of vineyard groundcover in 2016, whereas only one generation was observed in 2017. There appears to be an inverse relationship between the number of S. festinus adults sampled on ground cover and the number of girdles in the grapevine canopy. Spissistilus festinus exhibited an aggregated distribution in the vineyard and a significant edge effect. Results from this study will contribute to the development of sampling and management guidelines and determine timing of control measures to reduce populations of S. festinus within vineyards to minimize the virus spread. [ABSTRACT FROM AUTHOR]

Grapevine leafroll-associated virus 3 (GLRaV-3) is the most widely prevalent and economically important of the complex of RNA viruses associated with grapevine leafroll disease (GLD). Phylogenetic studies have grouped GLRaV-3 isolates into nine different monophyletic groups and four supergroups, making GLRaV-3 a genetically highly diverse virus species. In addition, new divergent variants have been discovered recently around the world. Accurate identification of the virus is an essential component in the management and control of GLRaV-3; however, the diversity of GLRaV-3, coupled with the limited sequence information, have complicated the development of a reliable detection assay. In this study, GLRaV-3 sequence data available in GenBank and those generated at Foundation Plant Services, University of California-Davis, was used to develop a new RT-qPCR assay with the capacity to detect all known GLRaV-3 variants. The new assay, referred to as FPST, was challenged against samples that included plants infected with different GLRaV-3 variants and originating from 46 countries. The FPST assay detected all known GLRaV-3 variants, including the highly divergent variants, by amplifying a small highly conserved region in the 3’ untranslated terminal region (UTR) of the virus genome. The reliability of the new RT-qPCR assay was confirmed by an enzyme linked immunosorbent assay (ELISA) that can detect all known GLRaV-3 variants characterized to date. Additionally, three new GLRaV-3 divergent variants, represented by four isolates, were identified using a hierarchical testing process involving the FPST assay, GLRaV-3 variant-specific assays and high-throughput sequencing analysis. These variants were distantly related to groups I, II, III, V, VI, VII and IX, but much similar to GLRaV-3 variants with no assigned group; thus, they may represent new clades. Finally, based on the phylogenetic analysis, a new GLRaV-3 subclade is proposed and named as group X. [ABSTRACT FROM AUTHOR]

Grapevine red blotch virus (GRBV) currently poses a serious threat to the wine industry. The three-cornered alfalfa hopper (3CAH), Spissistilus festinus (Say) (Hemiptera: Membracidae), was recently shown in a greenhouse study to be a vector of GRBV in grapes. Feeding and reproductive status of 10 weed and 10 cover crop species commonly found in grape vineyards were evaluated in no-choice tests. Species confirmed as reproductive hosts were subsequently evaluated in choice tests to determine S. festinus oviposition preference. Spanish clover, dandelion, birdsfoot trefoil, common groundsel, field bindweed, magnus peas, bell beans, blando brome, purple vetch, black medick, subterranean clover, crimson clover, and woollypod vetch were all found to be reproductive hosts. The plant species that exhibited the greatest nymph emergence in each of the three groups tested for preference were all in the family Fabaceae. Knowledge of plant species present in vineyards that serve as alternative hosts for S. festinus can contribute to developing management strategies to mitigate virus spread. [ABSTRACT FROM AUTHOR]

Copyright of Canadian Journal of Plant Pathology is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Canadian Journal of Plant Pathology is the property of Taylor & Francis Ltd and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Grapevine red blotch virus (GRBV) has become widespread in the United States since its identification in 2012. GRBV is the causative agent of grapevine red blotch disease (GRBD), which has caused detrimental economic impacts to the grape and wine industry. Understanding viral function, plant–pathogen interactions, and the effects of GRBV on grapevine performance remains essential to developing potential mitigation strategies. This comprehensive review examines the current body of knowledge regarding GRBV, to highlight gaps in the knowledge and potential mitigation strategies for grape growers and winemakers. [ABSTRACT FROM AUTHOR]

A novel cucumber mosaic virus inducible viral amplicon (CMViva) expression system has been developed that allows for tightly regulated chemically inducible expression of heterologous genes in plant hosts. Transient production of recombinant α1-antitrypsin (rAAT), a human blood protein, was demonstrated in Nicotiana benthamiana leaves. The highest production levels were obtained by co-infiltrating leaves with Agrobacterium tumefaciens cells containing CMViva carrying the AAT gene and A. tumefaciens cells carrying a binary vector constitutively expressing the gene silencing suppressor p19. Accumulation of up to thirty-fold more rAAT was observed in leaves (24 mg per 100 g leaf tissue) when compared with the expression levels observed using the cauliflower mosaic virus (CaMV) 35S promoter. Significantly, 70% of the rAAT produced using the CMViva expression system was found to be biologically active, a 170-fold increase in functional protein compared with the CaMV 35S expression system. [ABSTRACT FROM AUTHOR]

Feeding by the three-cornered alfalfa hopper, Spissistilus festinus (Say) (Hemiptera: Membracidae) results in girdling of grapevine petioles and shoots. Its significance as an economic pest of grape has increased since it was shown to transmit Grapevine red blotch virus (GRBV) in a greenhouse study. However, the status of grapevines as a reproductive host for S. festinus remained undetermined. Adult S. festinus were caged onto three regions of the grapevines: apical shoot, green shoot, and dormant cane. Their ability to reproduce was determined by weekly destructive sampling for 7 wk. Successful oviposition and nymphal emergence were observed on apical and green shoots, but not on dormant canes. However, insect development beyond the second nymphal instar did not occur. Knowledge of S. festinus reproduction on grapevines will be an important consideration in designing management guidelines to minimize the spread of GRBV in vineyards. [ABSTRACT FROM AUTHOR]