1. Expression of Low Endotoxin 3- O-Sulfotransferase in Bacillus subtilis and Bacillus megaterium.
- Author
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Wang, Wenya, Englaender, Jacob, Xu, Peng, Mehta, Krunal, Suwan, Jiraporn, Dordick, Jonathan, Zhang, Fuming, Yuan, Qipeng, Linhardt, Robert, and Koffas, Mattheos
- Abstract
A key enzyme for the biosynthesis and bioengineering of heparin, 3- O-sulfotransferase-1 (3-OST-1), was expressed and purified in Gram-positive Bacillus subtilis and Bacillus megaterium. Western blotting, protein sequence analysis, and enzyme activity measurement confirmed the expression. The enzymatic activity of 3-OST-1 expressed in Bacillus species were found to be similar to those found when expressed in Escherichia coli. The endotoxin level in 3-OST-1 from B. subtilis and B. megaterium were 10-10-fold lower than that of the E. coli-expressed 3-OST-1, which makes the Bacillus expression system of particular interest for the generation of pharmaceutical grade raw heparin from nonanimal sources. [ABSTRACT FROM AUTHOR]
- Published
- 2013
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