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Currently, spatiotemporal theranostic nanoprobes are in great demand, owing to their enhanced target therapy and precise dynamic tracing of in vivo drug fate. Herein, this study highlights the successful development of dynamic theranostic nanoprobes, which are facilely established via self‐assembly between glutathione (GSH)‐responsive dasatinib (DAS) dimers and indocyanine green (ICG). The DAS dimers endow the nanoprobes with aggregation‐induced emission (AIE) characteristic, whose emission wavelength successfully redshifts from 420 to 810 nm compared to DAS‐based nanoprobes, the same as that of ICG, thus improving the total fluorescence intensity. Moreover, the nanoprobes exhibit a dynamic fluorescence intensity conversion that first decreases and then increases at the tumor site via intracellular GSH‐triggered AIE quenching and fluorescence re‐enhancement of ICG, therefore achieving precise tumor diagnosis, prognosis evaluation, and spatiotemporal tracing of drug fate compared to other imaging strategies. Furthermore, the nanoprobes show long‐term circulation stability via suitable particle sizes and zeta potentials, improved tumor accumulation via extracellular protonation and active cellular uptake, efficient drug release via response to the intracellular milieu, and enhanced apoptosis via targeting to intracellular kinase, therefore achieving the significant tumor inhibition. Thus, the spatiotemporal theranostic nanoprobes can dynamically monitor the targeted tumor therapy, greatly advancing their application in clinics. [ABSTRACT FROM AUTHOR]

Aim: Ammonia‐oxidizing archaea (AOA) and bacteria (AOB) are the primary agents for nitrification, converting ammonia (NH4+) into nitrate (NO3−) and modulating plant nitrogen (N) utilization and terrestrial N retention. However, there is still lack of a unifying framework describing the patterns of global AOA and AOB distribution. In particular, biotic interactions are rarely integrated into any of the conceptual models. Location: World‐wide. Time period: 2005–2016. Major taxa studied: Ammonia‐oxidizing archaea and ammonia‐oxidizing bacteria. Methods: A meta‐analysis and synthesis were conducted to obtain a general picture of global AOA and AOB distribution and identify the primary driving factors. A microcosm experiment was then conducted to assess effects of relative carbon to nitrogen availability for heterotrophic microbes on AOA and AOB in two distinct soils. A mesocosm experiment was further carried out to characterize the effects of plant roots and their arbuscular mycorrhizal fungi (AMF) on AOA and AOB abundances using hyphae‐ or root‐ingrowth techniques. Results: Our meta‐analysis showed that soil carbon to nitrogen (C/N) ratios explained the most variance in AOA and AOB abundances, although soil pH had a significant effect. Experimental results demonstrated that high cellulose and mineral N inputs increased total microbial biomass and microbial activities, but inhibited AOA and AOB, suggesting microbial inhibition of AOA and AOB. Also, AMF and roots suppressed AOA and AOB, respectively. Main conclusions: Our study provides convincing evidence illustrating that relative carbon to nitrogen availability can predominantly affect the abundances of AOA and AOB. Our experimental results further validate that biotic competition among plants, heterotrophic microbes and ammonia oxidizers for substrate N is the predominant control upon AOA and AOB abundances. Together, these findings provide new insights into the role of abiotic and biotic factors in modulating terrestrial AOA and AOB abundances and their potential applications for management of nitrification in an increasing reactive N world. [ABSTRACT FROM AUTHOR]

The Loess Plateau soil in northwest China originated from wind sediments and is characterized by deep soil profiles and large organic carbon (C) content. Severe soil erosion constantly exposes deep soils to the surface, making the organic C vulnerable to microbial decomposition. Few, however, have so far examined how soil microbial activity and community composition in the deep loess soil respond to perturbations. We examined microbial responses in three layers of a clay‐loam loess (topsoil, 0–20 cm; midsoil, 40–60 cm; subsoil, 80–100 cm) to substrate additions (0.8 g glucose‐C kg−1 soil) under two temperature regimes (25 and 35°C). Soil C:N ratio was significantly larger in the subsoil (20.3) than topsoil (7.4). Glucose addition significantly increased CO2 efflux during a 30‐day incubation period and the relative magnitude of the increase was four times larger in the subsoil than topsoil. The temperature sensitivity (Q10) of soil CO2 efflux increased significantly with soil depth in the absence of glucose addition (i.e., ambient soil), but it decreased under glucose addition. Also, glucose addition significantly increased phenol oxidase and peroxidase activities in the subsoil, which might contribute to the stimulation of microbial CO2 efflux. Composition of the microbial community was more affected by temperature increase in the topsoil, but more responsive to labile C addition in the subsoil. Together, these results indicated that the composition of soil communities and microbial activities in the topsoil and deep soil responded differently to warming and labile C input. Our findings suggest that organic C in deep loess soils can be highly sensitive to environmental changes, emphasizing the need for more long‐term monitoring and quantitative assessment of organic C release from this important C pool. Highlights: Microbial responses to labile C and warming were examined along a Loess Plateau soil profile.Microbial respiration was more responsive to C addition and warming in deep soil than topsoil.Microbial composition and activity were sensitive to temperature in the topsoil but to labile C in the subsoil.Climate change may facilitate CO2 efflux from deep Loess Plateau soils. [ABSTRACT FROM AUTHOR]