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Wheat, being a crucial global food crop, holds immense significance for food safety and agricultural economic stability, as the quality and condition of its grains are critical factors. Traditional methods of wheat grain detection are inefficient, and the advancements in deep learning offer a novel solution for fast and accurate grain recognition. This study proposes an improved deep learning model based on YOLOv8n, referred to as YOLO-SDL, aiming to achieve efficient wheat grain detection. A high-quality wheat grain dataset was first constructed, including images of perfect, germinated, diseased, and damaged grains. Multiple data augmentation techniques were employed to enhance the dataset's complexity and diversity. The YOLO-SDL model incorporates the ShuffleNetV2 architecture in its backbone and combines depthwise separable convolutions (DWConv) with the large separable kernel attention (LSKA) mechanism in its neck structure, significantly improving detection speed and accuracy while ensuring the model remains lightweight. The results indicate that YOLO-SDL achieves superior performance in wheat grain detection, balancing lightweight design and performance optimization. The model achieved a P of 0.942, R of 0.903, mAP50 of 0.965, and mAP50-95 of 0.859, with low computational complexity, making it suitable for resource-constrained environments. These findings demonstrate the efficiency of the ShuffleNetV2, DWConv, and LSKA structures. The proposed YOLO-SDL model provides a new technical solution for agricultural automation and serves as a reliable reference for detecting other crops. [ABSTRACT FROM AUTHOR]

The degree of proteolytic modification in wheat malts significantly affects their quality, determining their suitability for use in brewing. Nitrogen fertilization at doses of 60 and 80 kg N·ha−1 applied during 3-year field experiments had a positive effect on the content of protein compounds in the analyzed wheat malts. The total protein content of the malts obtained averaged 9.9–10.1% d. m., depending on the cultivar, and from 9.1 to 10.8% dry matter, depending on nitrogen fertilization. The soluble protein content and Kolbach index value were at similar levels (4.60% dry matter and 46.07%, respectively, on average) except for malt obtained from the Elixer cultivar, for which significantly lower values were obtained (by 7.39% and 6.66%, respectively). In contrast, the highest free amino nitrogen value was obtained for malts derived from the Elixer and Gimantis cultivars. The least variation in the quality of malts derived from grains obtained in the three growing seasons during the field experiments was in the cultivar Gimantis. In 2-year commodity field experiments (in commodity farms), under 60 kg N·ha−1 nitrogen fertilization, the cultivar Lawina was characterized by the best indices of the degree of proteolytic modification. [ABSTRACT FROM AUTHOR]

Currently, the EU is focusing on less intensive agrotechnology and sustainable development. It is important to minimize the occurrence of mycotoxins (including Fusariotixins) in food, and to monitor mycotoxin concentration in the food chain. Therefore, this study evaluated Fusarium mycotoxin contamination, specifically type A and B trichothecenes and ergosterol concentration, in wheat grain from a three-year field experiment (2019–2021) conducted at IUNG-PIB in Osiny (Poland), along with its byproducts (bran, flour, bread). Four wheat cultivars were grown under different farming systems: organic (ORG), integrated (INT), and conventional (CONV). Ergosterol was analyzed using HPLC with an absorbance detector while Type A and B trichothecenes were analyzed using gas chromatography and mass spectrometry. Results showed that the farming system significantly influenced type B trichothecenes concentration in grain, with the highest concentration established in ORG-grown wheat. However, the grain concentration from the INT farming system was comparable to that from CONV. Type A trichothecenes concentrations were low and not significantly affected by the farming system. Bran exhibited the highest ergosterol and mycotoxin concentration, while flour and bread exhibited the lowest. [ABSTRACT FROM AUTHOR]

Cardiovascular diseases pose a major threat to both life expectancy and quality of life worldwide, and a concerning level of disease burden has been attained, particularly in middle- and low-income nations. Several drugs presently in use lead to multiple adverse events. Thus, it is urgently needed to develop safe, affordable, and effective management of cardiovascular diseases. Emerging evidence reveals a positive association between polyphenol consumption and cardioprotection. Whole wheat grain and allied products are good sources of polyphenolic compounds bearing enormous cardioprotective potential. Polyphenolic extract of the entire wheat grain contains different phenolic compounds viz. ferulic acid, caffeic acid, chlorogenic acid, p-coumaric acid, sinapic acid, syringic acid, vanillic acid, apigenin, quercetin, luteolin, etc. which exert cardioprotection by reducing oxidative stress and interfering with different toxicological processes. The antioxidant capacity has been thought to exert the cardioprotective mechanism of wheat grain polyphenolics, which predominantly suppresses oxidative stress, inflammation and fibrosis by downregulating several pathogenic signaling events. However, the combined effect of polyphenolics appears to be more prominent than that of a single molecule, which might be attained due to the synergy resulting in multimodal cardioprotective benefits from multiple phenolics. The current article covers the bioaccessibility and possible effects of wheat-derived polyphenolics in protecting against several cardiovascular disorders. This review discusses the mechanistic pharmacology of individual wheat polyphenols on the cardiovascular system. It also highlights the comparative superiority of polyphenolic extracts over a single phenolic. [ABSTRACT FROM AUTHOR]

This study was designed to assess the impacts of a mixture of deoxynivalenol (DON) and ergot alkaloids (EAs) on growth performance, rumen function, blood parameters, and carcass traits of feedlot cattle. Forty steers (450 ± 6.0 kg) were stratified by weight and randomly allocated to 1 of 4 treatments; control-low (CON-L), control-high (CON-H) which contained low or high wheat screenings that lacked mycotoxins at the same level as the mycotoxin-low (MYC-L; 5.0 mg/kg DON, 2.1 mg/kg EA), and mycotoxin-high (MYC-H: 10 mg/kg DON, 4.2 mg/kg EA) diets that included wheat screening with mycotoxins. Steers were housed in individual pens for a 112-day finishing trial. Intake was 24.8% lower (P < 0.001) for MYC steers compared to CON steers. As a result, average daily gains of MYC steers were 42.1% lower (P < 0.001) than CON steers. Gain to feed ratio was also lower (P < 0.001) for MYC steers compared to CON steers. Platelets, alanine aminotransferase, globulins, and blood urea nitrogen were lower (P ≤ 0.008), and lymphocytes, glutathione peroxidase activity (GPx), and interleukin-10 (IL-10) were elevated (P ≤ 0.002) in MYC steers compared to CON steers. Hot carcass weights and backfat thickness were reduced (P < 0.001) in MYC steers, resulting in leaner (P < 0.001) carcasses and higher (P < 0.007) meat yield compared to CON steers. Results suggest that a mixture of DON and EAs negatively impacted health, performance, and carcass traits of feedlot steers, with the majority of this response likely attributable to EAs. However, more research is needed to distinguish the relative contribution of each mycotoxin to the specific responses observed. [ABSTRACT FROM AUTHOR]

The effect of high pressure (0.1–600 MPa) and pulsed electric field (E = 3 and 6 kV cm−1) pretreatments on the moisture absorption properties of non‐hulled wheat grains were investigated. Microwave sensor was used as a non‐invasive technique for determination of the moisture content. The results demonstrated that high pressure soaking (>100 MPa) of wheat grains significantly increased the water uptake. High pressure soaking (600 MPa) decreased the water uptake due to the starch gelatinization and as a result appearance of structural changes. Compared to control samples, PEF‐pretreated samples showed a sharp increase of water absorption at the initial process with a further reduction of equilibrium water content. Peleg's water absorption model was adapted to the measured data of non‐hulled wheat grains. Results showed a good prediction of Peleg's equation in all experiments, which gave the possibility for estimation of initial water absorption rate and equilibrium moisture content. [ABSTRACT FROM AUTHOR]

BACKGROUND: Wheat grain containers or silos can be perfect habitats for insects, which generate large economic losses to grain production. Natural alternatives to synthetic insecticides have grown in popularity because of health, economic and ecological issues. Diatomaceous earth is a natural compound that has an insecticide effect by enhancing an insect's dehydration with no toxicity on mammals including humans. The aim of this study is to confirm the effect of diatomaceous earth as an insecticide for the wheat grain pest, the red flour beetle Tribolium castaneum (Coleoptera: Tenebrionidae) and demonstrate its underlying mechanisms as an insecticide by open‐flow respirometry and scanning electron microscopy. RESULTS: Survival bioassays of T. castaneum revealed a dose‐dependent insecticide effect of diatomaceous earth. Gravimetric measurements showed that 2 days exposure to diatomaceous earth produces a significant increase of mass loss. Open‐flow respirometry measurements showed an increase of total water emission rate on insects due to an increase of both, respiratory and cuticular water loss. Our study revealed that diatomaceous earth produces an increase of insect's cuticle permeability, which is responsible for elevated cuticular water loss. Scanning electron microscopy images provided visual evidence of the lipid absorbent properties of diatomaceous earth particles, and showed a tendency for higher, although not significant, damaged area of the cuticle's surface from diatomaceous earth treated insects compared to control ones. CONCLUSION: With state‐of‐the art techniques like open‐flow respirometry and scanning electron microscopy, we demonstrated the underlying mechanism of diatomaceous earth as an insecticide and provided new cues for understanding the properties of the cuticle and its ecological importance. © 2024 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

Over the last decade, more and more attention has been paid to applications of non-thermal plasma in agriculture, where it is used to decontaminate various microorganisms and to improve the seed germination. In this study, we present the results of a newly developed point-to-ring NTP transient spark discharge apparatus (NTP), plasma activated water (PAW) and their combined treatment on Durum wheat and Common wheat grains under laboratory conditions. Transient spark discharge treatment was used as direct treatment while indirect treatment of wheat grains was performed by PAW produced in point-to-plane NTP transient spark apparatus. We found that the degree of grain surface decontamination was in order NTP > PAW > combined treatment. In the case of Durum wheat grain germination, all treatments increased germination with increasing exposure times, while in the case of Common wheat, PAW treatment and combined treatment did not significantly increase the grain germination. In conclusion, plasma treatment has enormous potential for use in agriculture and its possibilities need to be fully explored. [ABSTRACT FROM AUTHOR]

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Functional foods enriched with plant polyphenol anthocyanins attract particular attention due to their health-promoting properties, including antitumor activity. We evaluated the effects of a grain diet rich in anthocyanins in a mouse model of Lewis lung carcinoma. Mice of the C57BL/6 strain were fed with wheat of near-isogenic lines differing in the anthocyanin content for four months prior to tumor transplantation. Although a significant decrease in the size of the tumor and the number of metastases in the lungs was revealed in the groups with both types of grain diet, the highest percentage of animals without metastases and with attenuated cell proliferation in the primary tumor were observed in the mice with the anthocyanin-rich diet. Both grain diets reduced the body weight gain and spleen weight index. The antitumor effects of the grain diets were associated with the activation of different mechanisms: immune response of the allergic type with augmented interleukin(IL)-9 and eotaxin serum levels in mice fed with control grain vs. inhibition of the IL-6/LIF system accompanied by a decrease in the tumor-associated M2 macrophage marker arginase 1 gene mRNA levels and enhanced autophagy in the tumor evaluated by the mRNA levels of Beclin 1 gene. Thus, anthocyanin-rich wheat is suggested as a promising source of functional nutrition with confirmed in vivo antitumor activity. [ABSTRACT FROM AUTHOR]

The role of lncRNA and circRNA in wheat grain development is still unclear. The objectives of this study were to characterize the lncRNA and circRNA in the wheat grain development and to construct the interaction network among lncRNA, circRNA, and their target miRNA to propose a lncRNA–circRNA–miRNA module related to wheat grain development. Full transcriptome sequencing on two wheat varieties (Annong 0942 and Anke 2005) with significant differences in 1000-grain weight at 10 d (days after pollination), 20 d, and 30 d of grain development were conducted. We detected 650, 736, and 609 differentially expressed lncRNA genes, and 769, 1054, and 1062 differentially expressed circRNA genes in the grains of 10 days, 20 days and 30 days after pollination between Annong 0942 and Anke 2005, respectively. An analysis of the lncRNA–miRNA and circRNA–miRNA targeting networks reveals that circRNAs exhibit a more complex and extensive interaction network in the development of cereal grains and the formation of grain shape. Central to these interactions are tae-miR1177, tae-miR1128, and tae-miR1130b-3p. In contrast, lncRNA genes only form a singular network centered around tae-miR1133 and tae-miR5175-5p when comparing between varieties. Further analysis is conducted on the underlying genes of all target miRNAs, we identified TaNF-YB1 targeted by tae-miR1122a and TaTGW-7B targeted by miR1130a as two pivotal regulatory genes in the development of wheat grains. The quantitative real-time PCR (qRT-PCR) and dual-luciferase reporter assays confirmed the target regulatory relationships between miR1130a-TaTGW-7B and miR1122a-TaNF-YB1. We propose a network of circRNA and miRNA-mediated gene regulation in the development of wheat grains. [ABSTRACT FROM AUTHOR]

Fusarium graminearum (F. graminearum) is a severe pathogen threatening the safety of agriculture and food. This study aimed to explore the antifungal efficacies of several plant-derived natural compounds (vanillin and its derivatives) against the growth of F. graminearum and investigate the antifungal mechanism of 2-hydroxy-4-methoxybenzaldehyde (HMB), the strongest one. The minimum inhibitory concentration (MIC) of HMB in inhibiting mycelial growth was 200 µg/mL. HMB at MIC damaged cell membranes by increasing the permeability by about 6-fold (p < 0.05) as evidenced by propidium iodide (PI) staining. Meanwhile, the content of malondialdehyde (MDA) and glycerol was increased by 45.91 and 576.19% by HMB treatment at MIC, respectively, indicating that lipid oxidation and osmotic stress occurred in the cell membrane. Furthermore, HMB exerted a strong antitoxigenic role as the content of deoxynivalenol (DON) was remarkably reduced by 93.59% at MIC on 7th day. At last, the antifungal effect of HMB against F. graminearum was also confirmed on wheat grains. These results not only revealed the antifungal mechanism of HMB but also suggested that HMB could be applied as a promising antifungal agent in the preservation of agricultural products. [ABSTRACT FROM AUTHOR]

A new approach to determining the friabilin content of wheat grain was proposed. Electropherograms were taken, and the intensity of the friabilin bands was compared in the analyzed wheat cultivars and the cv. Chinese Spring. The friabilin content indicator was calculated in the grain of 17 common wheat cultivars, which differed mostly in their crude protein content and hardness index (HI). The basic properties of the kernels were measured in each wheat cultivar, and the correlations between the measured parameters and the friabilin content indicator were determined. In the analyzed wheat cultivars, the friabilin content indicator ranged from around 0.21 to around 0.77. This indicator was significantly correlated with the kernel length, thickness, mass, vitreousness, HI, and rupture force. The strongest correlation was observed between the friabilin content indicator and kernel length. An increase in the mean kernel length from around 5.4 mm to around 8.0 mm decreased the friabilin content indicator by approximately 51%. After the mean kernel length had been calculated in a given wheat cultivar, a certain value of the friabilin content indicator could be ascribed to this cultivar, and the energy consumption during grain grinding or milling could be partly predicted. In the group of analyzed wheat cultivars, the process of grain grinding would be the most energy-intensive in the cvs. Ceres, SMH200, and SMH214 and the least energy-intensive in the cvs. Chinese Spring, Julius, and Askalon. [ABSTRACT FROM AUTHOR]

The partial substitution of chemical fertilizers with organic manure is an important strategy for improving agricultural sustainability. However, its effect on heavy metal (HM) pollution and its potential risk to human health remain unclear. Therefore, a field study was conducted to evaluate the effects of partial organic fertilizer substitution (0, 10%, 20%, 30%, and 40%) on health risks posed by HMs, including Zn, Cu, Ni, Cd, Pb, As, and Cr. The results showed that organic fertilizer substitution significantly increased Cu, Cd, Pb, and As accumulations in the soil. The Zn, Cu, Pb, and As contents were significantly higher in grains grown under organic fertilizer substitution (40%) conditions. The HM contents in the soil and grains were below the safety threshold limits in all treatments. Furthermore, the health risk caused by the exposure to As, Cu, and Zn accounted for 86% of the hazard index (HI) value. The HI value was significantly greater at a substitution ratio of 40% than in the no-nitrogen fertilizer treatment. Ingestion of the wheat grains grown at substitution ratios ≥ 30%) increased the carcinogenic risk of As and the total carcinogenic risk. In conclusion, organic fertilizer substitution at high ratios significantly increased the non-carcinogenic and carcinogenic risks associated with ingesting wheat grain. The optimal organic fertilizer substitution ratio (20%) maintained crop yields and improved soil fertility without increasing the non-carcinogenic or carcinogenic risks to human health. These findings highlight the importance of understanding the impacts of optimal organic fertilizer management in wheat growing systems. [ABSTRACT FROM AUTHOR]

In order to extend grain's storage time and ensure its quality, it is necessary to sort and clean it. The aim of this study was to justify the rational shape of the sorting chamber and the optimal technological parameters for the sorting of wheat grains in airflow. This study used newly designed grain sorting chambers with constant, widening, and narrowing cross-sections for the airflow sorting of "Skagen" wheat grain. The aerodynamic properties of wheat grains were investigated when moisture was at 14 ± 2.0%. The grain flow rate in the chambers varied from 4 to 12 kg min−1 every 2 kg min−1. In addition, the airflow velocity varied from 8 to 12 m s−1 every 1 m s−1. The tilt angle of the constant cross-section camera was increased to 5°. Experimental studies have determined a terminal airflow velocity of 11.53 m s−1 for wheat grains. At the terminal airflow velocity, the grain flight coefficient was obtained to be about 0.074. These studies showed that the narrowing chamber is preferable for lower grain flow rates compared to the constant cross-section of the chamber. The widening chamber requires a lower airflow velocity to achieve the same performance and quality as the other chambers. [ABSTRACT FROM AUTHOR]

Agronomic biofortification of crops is a promising approach that can improve the nutritional value of staple foods by alleviating dietary micronutrient deficiencies. Iodine deficiency is prevalent in many countries, including Australia, but it is not clear what foliar application strategies will be effective for iodine fortification of grain. This study hypothesised that combining adjuvants with iodine in foliar sprays would improve iodine penetration in wheat, leading to more efficient biofortification of grains. The glasshouse experiment included a total of nine treatments, including three reference controls: 1) Water; 2) potassium iodate (KIO3) and 3) potassium chloride (KCl); and a series of six different non-ionic surfactant or oil-based adjuvants: 4) KIO3 + BS1000; 5) KIO3 + Pulse® Penetrant; 6) KIO3 + Uptake®; 7) KIO3 + Hot-Up®; 8) KIO3 + Hasten® and 9) KIO3 + Synerterol® Horti Oil. Wheat was treated at heading, and again during the early milk growth stage. Adding the organosilicon-based adjuvant (Pulse®) to the spray formulation resulted in a significant increase in grain loading of iodine to 1269 µg/kg compared to the non-adjuvant KIO3 control at 231µg/kg, and the water and KCl controls (both 51µg/kg). The second most effective adjuvant was Synerterol® Horti Oil, which increased grain iodine significantly to 450µg/kg. The Uptake®, BS1000, Hasten®, and Hot-Up® adjuvants did not affect grain iodine concentrations relative to the KIO3 control. Importantly, iodine application and the subsequent increase in grain iodine had no significant effects on biomass production and grain yield relative to the controls. These results indicate that adjuvants can play an important role in agronomic biofortification practices, and organosilicon-based products have a great potential to enhance foliar penetration resulting in a higher translocation rate of foliar-applied iodine to grains, which is required to increase the iodine density of staple grains effectively. [ABSTRACT FROM AUTHOR]

The article presents the data on the quantitative and qualitative composition of micromycetes of wheat grain grown in the Polissya region. During the research period, an average of 2.88-104 ± 3.62-103 colony forming units (CFU) per 1 g of grain was found in wheat grain samples collected in the Polissya region. Twenty species of microscopic fungi belonging to 9 genera were isolated from the wheat grain of the Polissya zone. Among them were the genera Alternaria (92.5 %), Mucor (92.5 %), Aspergillus (83.0 %), Penicillium (47.2 %), Fusarium (60.4 %), Phoma (15,.1 %), Mycelia (15.1 %), Trichotecium (1.9 %) and Monascus (1.9 %) of the samples. Aspergillus niger (17.0 %), Aspergillus candidus (9.4 %), and Aspergillus terreus (1.9 %) were detected less frequently among Aspergillus. Fusarium spp. (17.0 %), Fusarium oxysporum, Fusarium moniliforme, Fusarium semitectum in (5.7 %), and Fusarium culmorum (3.8 %) of the samples were identified among Fusarium. A smaller number of microscopic fungi species represents the endophytic mycobiota of wheat grain. Among the isolates of micromycetes isolated from the Polissya zone, pure cultures were obtained from F. sporotrichiella var. tricinctum isolate 1218/4, and F. sporotrichiella isolate 1218/5. These isolates were atoxic against the test culture Candida pseudotropicalis strain 44 PC, but F. sporotrichiella var. tricinctum 1218/4 produced a growth retardation zone with Rf 0.05 and produced an unidentified trichothecene mycotoxin (TTMT). Among the isolated fungi, Aspergillus flavus isolate 1219/3 and Aspergillus flavus isolate 1221/1 were the first to produce kojic and aspergillic acids and the second to synthesize penicillic and aspergillic acids. To reduce the negative effect of the mycotoxin deoxynivalenol on the body of chickens of the meat and egg breed Adler Silver, the birds were fed the sorbent "Mikosorb". It was found that when added to the diet, the sorbent "Mikosorb" in the amount of 2.0 % of the total feed weight reduces the negative effect of deoxynivalenol on the body of chickens of the experimental group. This was confirmed by a 12.0 % reduction in bird mortality. Feeding "Microsorb" in the amount of 2.0 % by weight of complete feed contributed to an increase in the average daily weight gain of poultry during the experiment by 5.43 % compared to the experimental group that consumed feed with the toxin deoxynivalenol (DON). During the experiment, the birds of the experimental group No. 2 consumed 28.91 kg of feed more than group No. 2. Feeding the sorbent "Mikosorb" in the amount of 2.0 % of the feed weight to chickens of the meat and egg breed Adler silver contributes to an increase in gross weight gain by 9.69 kg during the experiment. The use of "Mikosorb" in the technology of feeding chickens of experimental group No. 2 contributed to an increase in the profitability of poultry production by up to 12.0 % compared to poultry that consumed feed affected by DON toxin. After analyzing the scientific results from the literature and our research results, we concluded that the abovementioned studies on wheat grain should be carried out throughout Ukraine during harvesting and storage in warehouses or storages. [ABSTRACT FROM AUTHOR]

Purpose: This work was based on a 40-year fertilization experiment to reveal the effect of organic amendments on the accumulation of heavy metals in soils and wheat grains. The results indicated that long-term application of organic amendments changed the accumulation of heavy metals in grains mainly by affecting soil chemical properties and wheat yields rather than soil heavy metals. Organic amendments have been widely used in agricultural practices to improve soil quality and crop yield, but it may lead to heavy metals (HMs) accumulation in the soil when livestock manure is applied. Currently, reports on the effects of organic amendments on HMs enrichment in crop grains are conflicting. Materials and methods: Soils and wheat grains were collected from a 40-year fertilization experiment in the Huang-Huai-Hai Plain in China to determine the concentrations of Cu, Zn, Cd, and As under different fertilization regimes. Additionally, soil chemical properties, available HMs contents, and wheat yields were measured to identify the main factors controlling grain HMs accumulation. Results and discussion: The results showed that, compared to non-fertilized soil, the 40-year application of swine manure markedly increased soil Cu and Zn by 113.5% and 92.3%, respectively; and cattle manure increased soil Cu, Zn, and Cd by 19.2%, 16.7%, and 102.6%, respectively. Straw amendment led to lower Zn content in the soil. Conversely, the concentrations of Cu, Zn, and As in wheat grains were significantly reduced by the application of organic amendments, whereas higher grain Cd was observed in the straw amendment treatment. The partial least squares path model indicated that wheat yield was positively correlated with soil chemical properties, and thus had a strong negative effect on grain Cu, Zn, and As (direct path coefficient = − 0.8541), whereas soil HMs had relatively weak effects. Redundancy analysis further illustrated that alkali-hydrolyzed nitrogen was the primary influencing factor among soil chemical properties, which explained 44.2% of the variation in grain HMs and negatively correlated with the concentrations of Cu, Zn, and As in wheat grains. Soil pH was negatively correlated grain Cd concentration and explained 40.8% of the grain HMs variation. Conclusions: Long-term application of livestock manure reduces the accumulation of Cu, Zn, and As in wheat grains by promoting soil chemical properties and wheat yields, while straw amendment increases grain Cd mainly due to lower soil pH. Soil HMs had weak effect on grain HMs. [ABSTRACT FROM AUTHOR]

This article deals with the problem of loose materials, i.e., wheat grain, when transported by belt conveyors with cover belts. For the purpose of further research, experimental measurements of the deflections of a conveyor belt with loose material were carried out on a self-built laboratory test device. The mechanical contact between the wheat and the belt could be suitably approximated using an elastic foundation. The measured data were evaluated and used to obtain functional relationships for the compressibility moduli of the bilateral Winkler elastic foundation. The obtained relationships were further stochastically processed using the Monte Carlo method. [ABSTRACT FROM AUTHOR]

This study optimised the conditions for ultrasound-assisted extraction (UAE) of defatted wheat germ protein (WGP) and evaluated its effect on the functional properties. Single-factor and orthogonal experiment designs were combined to optimise the UAE extraction condition. The extraction of WGP reached the highest level, at 88.66%, with a solid : liquid ratio of 1 : 25 g·mL-1, pH value of 9.0, ultrasonic time of 10 min, and ultrasonic power at 400 W. Under these conditions, albumin, globulin, prolamin, and glutenin accounted for 32.26, 28.52, 5.42, and 22.40% of total protein, respectively. In addition, this study compared the functional properties of WGP extracted by UAE with the results based on a commercially available soy protein (SP) isolate (SPI). The UAE of WGP had better oil absorption, foaming, and emulsifying properties. Therefore, UAE is a promising technique for food protein extraction because it can change the protein efficiencies and properties of the extract. [ABSTRACT FROM AUTHOR]

A deficiency of zinc (Zn) is ubiquitous causing threat of Zn malnutrition worldwide, especially, due to reliance on Zn-poor cereal-based diets. As a principal staple grain, wheat (Triticum aestivum L.) is consumed by 40% of global population. The wide gap between the available Zn concentration in wheat grain (20–35 mg kg−1) and that required Zn for human health (45 mg kg−1), urges the need for biofortification. Agronomic biofortification is a feasible and economic intervention strategy for mitigation of Zn deficiency or malnutrition by increasing Zn concentration and bioavailability in edible parts of cereals with increased yield; though there are bottlenecks at the root-shoot barrier and in grain filling. This review explores the reasons to enhance grain Zn bioavailability, and the role of fertilizer management on agronomic biofortification of wheat with Zn. Foliar, or soil + foliar application of Zn salts can increase the Zn concentration ≤60 mg kg−1 in whole wheat grain. The Zn-nanocarrier (Zn-Chitosan Nanoparticles) has higher Zn-use efficiency, than zinc sulfate. Grain Zn accumulation is regulated by Zn remobilization from shoot and continuous uptake during the grain filling of wheat. Foliar Zn application early during grain filling improves Zn transport in the endosperm, which is the main consumable grain fraction. Relevantly, newer-released cultivars exhibit Zn-Fe antagonism, may challenge a combined loading of Zn and Fe in cereal grains, but biofortification does not intend a trade-off between two. Consequently, the efficacy of Zn-biofortification needs to be studied using distinct biomarkers. [ABSTRACT FROM AUTHOR]

Some wheat cultivars show a linear relationship between grain protein concentration (GPC) and baking volume, but others display a saturation curve. Such a saturation curve could be general, but in some cultivars it might only appear at GPC > 17%. However, such GPC is mostly not achieved in the field. Pot experiments with high nitrogen application reliably result in GPC > 17%. In a pot experiment with a high (N1) and an excessive N level (N2) and four cultivars (Akteur, Arnold, Discus and Hystar), the change in grain protein composition and the relationship between different protein fractions and baking volume at GPC > 17% was investigated. GPC ranged from 17 to 24% and mean nitrogen content per grain from 1.2 to 1.8 mg. The N2 treatment increased GPC and mean nitrogen content per grain in the Akteur and Discus cultivar, but not in Arnold and Hystar. N2 increased concentration of gliadin by 10 to 34% and glutenin macropolymer (GMP) in all cultivars by 12 to 73%. Glutenin concentration was increased by N2 in Akteur and Discus (19 to 36%), but was decreased by N2 in the Arnold and Hystar cultivar. Baking volume was moderately increased by N2 in all cultivars by 6 to 9% and correlated significantly with most glutenin fractions in the Akteur and Discus cultivar, with GMP in Arnold and with HMW-GS to LMW-GS ratio in Hystar. Thus, specific effects on grain protein by N2 were responsible for the increased baking volume in each cultivar. However, as gliadin and its sub-fractions hardly correlated with baking volume, a positive effect of increasing gliadin proteins on baking quality was not obvious. [ABSTRACT FROM AUTHOR]

Wheat plants are subject to the impact of a complex of pathogenic agents, which include over ten species of diverse etiological and pathological nature, as key objects of economic importance, that annually cause serious damage, such as: Ustilago tritici; Tilletia caries; T. foetida; Puccinia recondita; P. glumarum; P. graminis; Erysiphe graminis; Fusarium graminearum; Septoria tritici, S. graminum; Helminthosporium tritici-repentis. The fungicidal remedies Camporo 25 EC, Custodia 320 SC and Sizaro ES fungicides have been tested against the diseases detected in winter wheat plantations. The efficiency of these new remedies with anti-fungal action was proved depending on the doses applied and the severity of the disease, as compared with the standard control. Camporo 25 EC and Custodia 320 SC, Sizaro ES fungicides are recommended as efficient chemical products in the integrated protection system of winter wheat. The frequency and intensity of the attack of pathogens, in 2021-2022, ranged between 10 and 45%, depending on environmental factors, and the biological efficiency of the new remedies tested on experimental plots as fungicides was 90-93%, at the level of the standard variant, comparing the variants and doses applied. [ABSTRACT FROM AUTHOR]

Boring pests such as Sitophilus zeamais (S. zeamais) are major threats in grain storage. However, how these pests affect the proteins of stored grains remains largely unknown. Here we aimed to investigate the effect of S. zeamais infestation on wheat protein during postharvest storage. In this study, wheat grain infested by S. zeamais was sampled at egg (4 d), larval (20 d), pupal (35 d), and adult stages (45 d), respectively. The protein's physicochemical and structural properties and the edible quality of whole wheat noodle were analyzed. The results showed that S. zeamais infestation significantly decreased the quality of wheat protein by altering its constitution and structure properties. Especially, compared with the control, the content of wet and dry gluten, gluten index, sodium dodecyl sulfate sedimentation volume, sulfhydryl groups, and disulfide bonds in insect-infested wheat decreased by 19.40, 5.42, 18.40, 8.12, 29.13, and 14.30%, respectively, during the storage period of one life cycle of S. zeamais. Additionally, the proportions of wheat protein fractions (albumin [1.16-fold], globulin [0.96-fold], gliadin [1.16-fold], and glutenin [0.95-fold]) and secondary structures (α-helix [0.91-fold], β-fold [0.96-fold], β-turn [1.06-fold], and random coil [1.05-fold]) of protein changed significantly, and the gluten network structure was broken in S. zeamais-infested wheat. Furthermore, the color of whole wheat noodle became darker, cooking loss rate increased, and textural properties (hardness, adhesiveness, springiness, cohesiveness, chewiness, and resilience) decreased as well. The results in the present study provided new insights for analyzing the quality deterioration mechanism and further quality improvement of boring pests-infested wheat grain. [ABSTRACT FROM AUTHOR]

Sensitivity of reproductive stage to high temperature was investigated in twenty wheat genotypes (three tall traditional-C ones, seven elite-PBW ones, nine advanced-BWL ones and one landrace-Himachal Local 1) using various biochemical indices under high-temperature stress. Effect of high temperature on activities of antioxidant enzymes and contents of phenolic compounds in relation to transformation of free sugars to starch in developing grains of wheat genotypes was studied by raising the crop under timely sown (November) and late planting (December) conditions. During metabolomic profiling, it was confirmed that sugars, amino acids and proteins accumulated in different wheat genotypes under high temperature with decrease in starch content. Disruption of starch biosynthesis resulted in their favored utilization in nitrogen metabolism leading to higher amino acid and protein accumulation as depicted by the negative correlation between starch and proteins (p ≤ 0.05) under heat stress. Phenolic compounds, viz. total polyphenols, phenolic acids, flavonols, anthocyanins and proanthocyanidins, were increased under stress conditions. Increase in polyphenolic content could be associated with the activation of phenylalanine ammonia lyase enzyme involved in shikimic acid pathway in wheat grains. A significant correlation (p ≤ 0.01) of total polyphenols and proanthocyanidins was observed with the activity of catalase (r = 0.59) and polyphenol oxidase (r = 0.27), respectively. Advanced lines, namely BWL 1664, 3504 and 6250, performed better with less decrease in yield under heat stress which was attributed to low reduction in starch content. [ABSTRACT FROM AUTHOR]

The research was carried out in three vegetative seasons 2015/16, 2016/17, and 2018/19, on an experimental plot in Padinska Skela, Belgrade, Serbia. The supplement included four NPK treatments (kg ha−1) and foliar treatment with effective microorganisms (EM 7 l ha−1) T1-129: 60: 60; T2-129: 60: 60 + 1 EM; T3-106: 60: 60 + 2 EM ; T4-83: 60: 60 + 3 EM. Four wheat genotypes were sown (Ratarica, Pobeda, Nogal, and Apache). Thousand grain weight and grain yield were determined. The thousand grain weight (TGW) was in the variety Pobeda 43.73 g, and the highest grain yield was in the variety Ratarica 7.02 tha−1. Both varieties are medium late, bread varieties. The treatments have different effects on the studied variables. The TGW was the highest in the T2 treatment of 40.81 g and the grain yield in T3 of 6.62 t ha−1, which was at the level of statistical significance of p > 0.01. The crude protein content was highest in the treatment of T2 13.93% (p > 0.01). [ABSTRACT FROM AUTHOR]

The three-dimensional (3D) morphological information of wheat grains is an important parameter for discriminating seed health, wheat yield, and wheat quality. High-throughput acquisition of 3D indicators of wheat grains is of great importance for wheat cultivation management, genetic breeding, and economic value. Currently, the 3D morphology of wheat grains still relies on manual investigation, which is subjective, inefficient, and poorly reproducible. The existing 3D acquisition equipment is complicated to operate and expensive, which cannot meet the requirements of high-throughput phenotype acquisition. In this paper, an automatic, economical, and efficient method for the 3D morphometry of wheat grain is proposed. A line laser binocular camera was used to obtain high-quality point-cloud data. A wheat grain 3D model was constructed by point-cloud segmentation, finding, clustering, projection, and reconstruction. Based on this, 3D morphological indicators of wheat grains were calculated. The results show that the root mean square error (RMSE) and mean absolute percentage error (MAPE) of the length were 0.2256 mm and 2.60%, the width, 0.2154 mm and 5.83%, the thickness, 0.2119 mm and 5.81%, and the volume, 1.7740 mm3 and 4.31%. The scanning time was around 12 s and the data processing time was around 3.18 s under a scanning speed of 25 mm/s. This method can achieve the high-throughput acquisition of the 3D information of wheat grains, and it provides a reference for in-depth study of the 3D morphological indicators of wheat and other grains. [ABSTRACT FROM AUTHOR]

Excessive accumulation of heavy metal(loid)s in agricultural environment usually originates from anthropogenic activities. Both large diversities of emission sources and complexity of plant accumulation challenge the understanding of the site-specific effects of emission sources on heavy metal(loid)s in wheat grains. Herein, both soil samples and wheat grain samples (n = 80) were collected from the farmland of Jiyuan City, China. Soil and grain burdens of heavy metal(loid)s were determined by inductively coupled plasma mass spectrometry (ICP-MS) and/or X-ray fluorescence spectrometry (XRF). The quotients (Q) were developed to indicate relative impacts of industrial plants and traffic to soil sites. Principal component analysis-absolute principal component scores-multivariate linear regression (PCA-APCS-MLR) analysis was conducted to reveal the source contributions to heavy metal(loid)s in grains, considering Q values, soil, and wheat grain data. Results showed that contributions of main sources and factors drastically varied with soil sites, and usually overlapped to different extents. For grain Cd and grain Pb, natural soil silicate (0.066/0.104 mg/kg) and iron-bearing minerals (− 0.044/ − 0.174 mg/kg) contributed to high extents, while metal smelting activities (0.018/0.019 mg/kg) and agronomic activities (− 0.017/ − 0.019 mg/kg) unexpectedly posed low or moderate contributions. The pH-mediated availability of soil Cd (0.035 mg/kg) and the sand-dust weather (0.028 mg/kg) also made considerable contributions to grain Cd. For grain As, both natural soil iron-bearing (− 0.048 mg/kg) and silicate minerals (− 0.013 mg/kg) made negative contributions. The results benefit to the decision-making of pollution remediation of farmland soils in the regional scales. [ABSTRACT FROM AUTHOR]

The object of the study is the treatment of wheat grain with an electromagnetic field (EMF) of extremely low frequencies (ELF), the subjects of the study are the quality indicators of wheat seed grain of the Shestopalivka variety 2019 and 2020 crops grown in the Odesa region (Ukraine). Problematic issues in the treatment of wheat grain with ELF EMF are the rationale for the duration of treatment of grain and the frequencies of EMF that improves the quality of seeds. The studies used methods of laboratory determination of seed quality indicators, calculation of statistical characteristics of the length of sprouts, and graphical methods for interpreting the results of studies. The studies substantiated the modes of treatment wheat grain with ELF EMF, which improves the quality of seeds and reduces the energy intensity of treatment. The effect of the duration of grain treatment and the frequency of EMF on the germination and characteristics of the length of seed shoots was studied. It has been established that, compared with untreated grain, the treatment of grain with an EMF with a frequency of 30 Hz, a magnetic induction of 10 mT for 6 minutes increases grain germination by 2–3 %, gives longer and 1.44–1.53 times more uniformly sprouted sprouts. Treatment within 60 min. reduces up to 9 % the germination of grain, the size of the shoots, increases their unevenness in length. The effect of seed germination activation by EMF treatment increases after 19 days of storage. Treatment of wheat grain in 2019 with ELF EMF at a frequency of 15–17 Hz with a magnetic induction of 10 mT for 6 min. changes germination within ±3 % control. The germination of the treated grain of wheat in 2020 of the crop decreases relative to the control to 13 % (with the exception of the frequency of 16.5 Hz, at which it did not change). Thus, the treatment of wheat grain with EMF makes it possible to influence the quality of seed grain. The results obtained encourage further research with a wider range of regime parameters and areas of wheat cultivation. [ABSTRACT FROM AUTHOR]

Copyright of Experimental Technology & Management is the property of Experimental Technology & Management Editorial Office and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Key message: TaPTST1, a wheat homolog of AtPTST1 containing CBM can interact with GBSSI and regulate starch metabolism in wheat endosperm. In cereal endosperm, native starch comprising amylose and amylopectin is synthesized by the coordinated activities of several pathway enzymes. Amylose in starch influences its physio-chemical properties resulting in several human health benefits. The Granule-Bound Starch Synthase I (GBSSI) is the most abundant starch-associated protein. GBSSI lacks dedicated Carbohydrate-binding module (CBM). Previously, Protein Targeting To Starch 1 (PTST1) was identified as a crucial protein for the localization of GBSSI to the starch granules in Arabidopsis. The function of its homologous protein in the wheat endosperm is not known. In this study, TaPTST1, an AtPTST1 homolog, containing a CBM and a coiled-coil domain was identified in wheat. Protein-coding nucleotide sequence of TaPTST1 from Indian wheat variety 'C 306' was cloned and characterized. Homology modelling and molecular docking suggested the potential interaction of TaPTST1 with glucans and GBSSI. The TaPTST1 expression was higher in wheat grain than the other tissues, suggesting a grain-specific function. In vitro binding assays demonstrated different binding affinities of TaPTST1 for native starch, amylose, and amylopectin. Furthermore, the immunoaffinity pull-down assay revealed that TaPTST1 directly interacts with GBSSI, and the interaction is mediated by a coiled-coil domain. The direct protein–protein interaction was further confirmed by bimolecular fluorescence complementation assay (BiFC) in planta. Based on our findings we postulate a functional role for TaPTST1 in starch metabolism by targeting GBSSI to starch granules in wheat endosperm. [ABSTRACT FROM AUTHOR]

Post-anthesis phosphorus (P) uptake and the remobilization of the previously acquired P are the principal sources of grain P nutrition in wheat. However, how the acquired P reaches the grains and its partitioning at the whole plant level remain poorly understood. Here, the temporal dynamics of the newly acquired P in durum wheat organs and its allocation to grain were examined using pulse-chase 32 P-labeling experiments at 5 and 14 days after anthesis. Durum wheat plants were grown hydroponically under high and low P supplies. Each labeling experiment lasted for 24 h. Plants were harvested 24, 48, and 96 h after labeling. Low and high P treatments significantly affected the allocation of the newly acquired P at the whole plant level. Three days (96 h) after the first 32 P-labeling, 8% and 4% of the newly acquired P from exogenous solution were allocated to grains, 73% and 55% to the remainder aboveground organs, and 19% and 41% to the roots at low and high P supplies, respectively. Three days after the second labeling, the corresponding values were 48% and 20% in grains, 44% and 53% in the remainder aboveground organs, and 8% and 27% in roots at low and high P supplies, respectively. These results reveal that the dynamics of P allocation to grain was faster in plants grown under low P supply than under high supply. However, the obtained results also indicate that the origin of P accumulated in durum wheat grains was mainly from P remobilization with little contribution from post-anthesis P uptake. The present study emphasizes the role of vegetative organs as temporary storage of P taken up during the grain filling period before its final allocation to grains. [ABSTRACT FROM AUTHOR]

The wheat grain three-dimensional (3D) phenotypic characters are of great significance for final yield and variety breeding, and the ventral sulcus traits are the important factors to the wheat flour yield. The wheat grain trait measurements are necessary; however, the traditional measurement method is still manual, which is inefficient, subjective, and labor intensive; moreover, the ventral sulcus traits can only be obtained by destructive measurement. In this paper, an intelligent analysis method based on the structured light imaging has been proposed to extract the 3D wheat grain phenotypes and ventral sulcus traits. First, the 3D point cloud data of wheat grain were obtained by the structured light scanner, and then, the specified point cloud processing algorithms including single grain segmentation and ventral sulcus location have been designed; finally, 28 wheat grain 3D phenotypic characters and 4 ventral sulcus traits have been extracted. To evaluate the best experimental conditions, three-level orthogonal experiments, which include rotation angle, scanning angle, and stage color factors, were carried out on 125 grains of 5 wheat varieties, and the results demonstrated that optimum conditions of rotation angle, scanning angle, and stage color were 30°, 37°, black color individually. Additionally, the results also proved that the mean absolute percentage errors (MAPEs) of wheat grain length, width, thickness, and ventral sulcus depth were 1.83, 1.86, 2.19, and 4.81%. Moreover, the 500 wheat grains of five varieties were used to construct and validate the wheat grain weight model by 32 phenotypic traits, and the cross-validation results showed that the R 2 of the models ranged from 0.77 to 0.83. Finally, the wheat grain phenotype extraction and grain weight prediction were integrated into the specialized software. Therefore, this method was demonstrated to be an efficient and effective way for wheat breeding research. [ABSTRACT FROM AUTHOR]

Copyright of Mesopotamia Journal of Agriculture is the property of Republic of Iraq Ministry of Higher Education & Scientific Research (MOHESR) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Ozonation of wheat grain is a quick and easy process that could be realized during the hydrating. Ozonated water with 75, 90 and 105 g/m³ ozone concentrates and the control (distilled water not treated with ozone) was used as a treatment with zero and all samples were used to wet the wheat grains for 24 hours (to reach 15% moisture) and then milled by laboratories miller with the extraction percentage were 82%. The results of physical and chemical properties of wheat flour were The ozone is in direct contact with the grain and modifies immediately the properties of the wheat. Stability andresistance of dough were increased with ozonation in comparative with control sample. Absorbance increased with the increase in the ozone concentration, and the increase in the water absorbency with an increase in the ozone concentration was significantly distinct at the level of 0.05, and it color of the flour was lighter in compared to unprocessed wheat flour. Ozone has a clear effect on fresh wheat flour produced from wheat treated with ozonation and in all concentrations. It was noted that there were no insect eggs in the ozonetreated wheat flour as it is a good sterilization agent that leads to the destruction of eggs and killing insects and their larvae, number of molds for fresh wheat flour resulting from ozone-treated grains numbers decrease with the increase in ozone concentration this is because ozone is an effective sterilization agent. Results showed a gradual increase in specific volume of local bread. [ABSTRACT FROM AUTHOR]

By using Bishop's formula to assess calculated extract potential of wheat grains at the stage of wholesale purchase of the material, it could be possible to determine the quality from the viewpoint of the brewing industry. Based on this, buyers could rapidly assess the material before purchase and malt-houses could optimize the technological process. This study examined the feasibility of Bishop's formula (originally designed to identify calculated extract of malting barley) being applied to measure the calculated extract of wheat. For this purpose, ten winter cultivars of common hexaploid wheats, grown in 2019, were selected for the study. The variables applied included the cultivar and the plumpness of the wheat grains. The grains were sorted into size 2.5-2.8 mm and size > 2.8 mm for the study of nitrogen content and 1,000 grain weight (TGW). The wheat was malted and five-day laboratory malts were obtained. The Congress mashing protocol was carried out and the actual extract of the wheat malts was determined based on the extract produced in that specific process. The actual extract values were compared to the calculated extract according to Bishop (statistical analysis). Small statistical differences were observed between the calculated and the actual extract of the wheat malts, however more research is needed to determine if Bishop's formula is as useful for wheat as it is for barley malt. [ABSTRACT FROM AUTHOR]

Wheat is one of the most important crops in the world, mainly used for human consumption and animal feed. To overcome the increasing demand in wheat production, it is necessary to better understand the mechanisms involved in the growth of the wheat grain. X-ray computed tomography is an efficient method for the non-destructive investigation of the 3D architecture of biological specimens, which does not require staining, sectioning, or inclusion. In particular, phase-contrast tomography results in images with better contrast and an increased resolution compared to that obtained with laboratory tomography devices. The aim of this study was to investigate the potential of phase-contrast tomography for the study of the anatomy of the wheat grain at early stages of development. We provided 3D images of entire grains at various development stages. The image analysis allowed identifying a large number of tissues, and to visualize individual cells. Using a high-resolution setup, finer details were obtained, making it possible to identify additional tissues. Three-dimensional rendering of the grain also revealed the pattern resulting from the epidermis cells. X-ray phase-contrast tomography appears as a promising imaging method for the study of the 3D anatomy of plant organs and tissues. [ABSTRACT FROM AUTHOR]

Summary: Protein abundance in cereal grains is determined by the relative rates of protein synthesis and protein degradation during grain development but quantitation of these rates is lacking.Through combining in vivo stable isotope labelling and in‐depth quantitative proteomics, we have measured the turnover of 1400 different types of proteins during wheat grain development.We demonstrate that there is a spatiotemporal pattern to protein turnover rates which explain part of the variation in protein abundances that is not attributable to differences in wheat gene expression. We show that c. 20% of total grain adenosine triphosphate (ATP) production is used for grain proteome biogenesis and maintenance, and nearly half of this budget is invested exclusively in storage protein synthesis. We calculate that 25% of newly synthesized storage proteins are turned over during grain development rather than stored.This approach to measure protein turnover rates at proteome scale reveals how different functional categories of grain proteins accumulate, calculates the costs of protein turnover during wheat grain development and identifies the most and the least stable proteins in the developing wheat grain. [ABSTRACT FROM AUTHOR]

The paper presents the results of studies of how the electromagnetic field (EMF) of extremely low frequencies (ELF), magnetic induction of the EMF, and the duration of processing and storage affect the quality characteristics of wheat grain of the cultivar Shestopalivka harvested in the Odesa Region in 2018, 2019, and 2020. Treatment of grain with the EMF of the frequencies 20 and 30 Hz and with magnetic induction of 5 and 10 mT for 2–20 min reduces the germination capacity of wheat, as compared with the control, by 1–12%. As an exception, in several experiments, the germination capacity increased. The biggest increase (by 14%) was observed when the duration of treatment was 12 minutes, the frequency 30 Hz, and the magnetic induction 5 mT. Treatment of grain for 6 min with the EMF at the frequencies 10–28 Hz and with magnetic induction showed that at 10, 18–24 Hz, the germination capacity increased by 1–5%, but after 15 days, at the frequency 12–26 Hz, the germination power increased by 1–9%. A month later, with the frequency 18, 20, and 28 Hz, the germination power exceeded the control by only 2–7%, and after 10 months of storage, only the experiment with the frequency of treatment 24 Hz was at the control level. With all other frequencies, there was a significant decrease in grain germination. It has been established that treating grain 15 days prior to sowing at 14–22 Hz increases the germination capacity by 7–9%, and it reaches the values 92–94%. In the grain treated with the EMF at 10–30 Hz and stored for 14 months at the controlled air temperature 9–23°C and the relative humidity 33–82.5%, the protein content on a dry basis did not change compared with the control (16.5%). Storage of grain treated for 6 minutes with an EMF at 10–30 Hz, the magnetic induction being 10 mT, allows increasing the gluten content by 1.07–1.21 times, as compared with the control. The biggest increase in the amount of gluten is achieved at the frequency 16 Hz. Even under unfavourable conditions, at the temperature 23°C and relative air humidity 82.5%, the gluten quality goes up by 27.65 conditional units of the device VDK (1.49 times) and becomes as high as the gluten quality of grain that was preserved under far better conditions, at 9°C and the humidity 33 %. When grain is EMF-treated for 6 min at 10–30 Hz, with the magnetic induction being 10 mT, under different storage conditions, this most often affects the content of stearic and gondoic acids. The biggest changes in them were noted during storage at 9°C and the relative air humidity 82%: gondoic acid increased by 12.5–18.7%, and stearic acid decreased by 15.5–25.0%. Under any other conditions of EMF treatment and storage of wheat grain, changes in fatty acids are within the allowable error. [ABSTRACT FROM AUTHOR]

To enlarge the applications of whole wheat grain (WWG) and wheat bran (WB) as functional ingredients in foodstuffs that can promote human health, researchers have explored bioprocessing approaches to improve the bioaccessibility of phenolic compounds from these food matrices and, subsequently, their biological effects. The objective of this study was to compare the composition in nutrients, anti-nutrients, and bioactive compounds of WWG and WB, and their respective bioprocessed products: sprouted wheat (GERM) and WB hydrolysate (stabilized by spray-drying [SPD] and microencapsulated [MEC]). In addition, to evaluate the functional properties of these ingredients, the bioaccessibility of phenolic compounds and their potential antioxidant and anti-inflammatory activities were monitored in different digestion steps. GERM had increased amounts of insoluble dietary fiber, higher diversity of oligosaccharides, and higher concentration of monosaccharides, free phosphorous, and phenolic compounds than WWG. SPD had improved content of soluble dietary fiber, oligosaccharides, monosaccharides, free phosphorous, and phenolic compounds (vs. WB), whereas MEC was mainly composed of protein and had nearly 2-fold lower content of SPD components. All the ingredients showed lower amounts of phytic acid as compared with raw materials. In all samples, hydroxycinnamic acids were the most representative polyphenols followed by minor amounts of hydroxybenzoic acids and flavonoids. Gastrointestinal digestion of GERM, SPD, and MEC revealed high stability of total phenolic compounds in both gastric and intestinal phases. Hydroxycinnamic acids were the most bioaccessible compounds during digestion among the three bioprocessed wheat ingredients studied, although their bioaccessibility varied across ingredients. In this sense, the bioaccessibility of ferulic acid (FA) derivatives increased in GERM with progression of the digestion, while it was reduced in SPD and MEC up to the end of the intestinal phase. Microencapsulation of SPD with pea protein led to generally to lower bioaccessible amounts of phenolic acids. Comparison analysis of biological effects highlighted SPD for its most potent antioxidant effects in the gastrointestinal tract (3 out 4 antioxidant parameters with highest values), while no clear differences were observed with regard to in vitro anti-inflammatory activity. Overall, these results support the potential application of GERM, SPD, and MEC as functional and nutraceutical ingredients. [ABSTRACT FROM AUTHOR]

Wheat (Triticum aestivum L.) is a widely cultivated crop and one of the most commonly consumed food grains in the world. It possesses several nutritional elements. Increasing attention to wheat grain phenolics bioactivity is due to the increasing demand for foods with natural antioxidants. To provide a comprehensive understanding of phenolics in wheat grain, this review first summarizes the phenolics' form and distribution and the phenolic components identified in wheat grain. In particular, the biosynthesis path for phenolics is discussed, identifying some candidate genes involved in the biosynthesis of phenolic acids and flavonoids. After discussing the methods for determining antioxidant activity, the effect of genotypes, environmental conditions, and cultivation systems on grain phenolic component content are explored. Finally, the bioavailability of phenolics under different food processing method are reported and discussed. Future research is recommended to increase wheat grain phenolic content by genetic engineering, and to improve its bioavailability through proper food processing. © 2021 Society of Chemical Industry. [ABSTRACT FROM AUTHOR]

The results of studies of the influence of the electromagnetic field (EMF) and the duration of wheat grain storage of varieties Guzel, Arista, Obrana and Shestopalivka on their technological and seed quality properties are presented. Grain processing was carried out by EMF with frequencies of 10...30 Hz with a magnetic induction of 10 mT for 6 minutes. It is shown that the processing of wheat grain with EMF increased the content of raw gluten by 1.0...3.2% for the Gyuzel variety and by 1.0...2.6% for the Obrana variety. For variety Arista, the content of crude gluten increased by 1.0% only when treated with EMF at a frequency of 16 Hz, and at other frequencies it decreased within the error. For these varieties of wheat, EMF treatment with a frequency of 16 Hz increases the amount of crude gluten, depending on the variety, by 1.0...3.2%, which increases the class of grain. However, grain processing with a frequency of 16 Hz reduces the quality of gluten in the Guzel and Arista varieties, and it passes from the 1st to the 2nd quality group. When storing wheat grain treated with EMF for about 8 months, the sedimentation index, which comprehensively characterizes the strength of flour, increases by 3 ml (by 4.6%) compared to the control only at a frequency of 16 Hz at a storage temperature of 9 °C and a relative humidity of 82%, and after storage for almost 14 months, it grows by 2 ml (by 3.1%). An increase in the sedimentation index by 6...9 ml (by 10.7...16.1%) was noted when processing grain with EMF frequencies of 10...30 Hz during storage for about 14 months at a temperature of 9 °C and a relative humidity of 33%. Under other storage conditions and EMF processing frequencies, the sedimentation index may not change or decrease to 13 ml (up to 20.0%), which indicates a deterioration in the quality of gluten. Studies of the falling number (FN) of wheat samples of varieties Guzel, Arista, Obrana and Shestopalivka showed that their EMF treatment changes the FN indicator only within acceptable deviations. The exception was a grain sample of the Shestopalivka variety treated with EMF at a frequency of 30 Hz. After its storage for 12.8 months at a temperature of 23 °C and a relative humidity of 35%, the FN index decreased by 52 seconds compared to the control. In samples of wheat varieties Guzel and Arista with a low initial after EMF treatment, germination decreased by 1.06...1.71 times compared with the control. In the wheat variety Obrana with an initial germination of 80% EMF treatment increased the germination rates. The greatest effect was obtained by processing grain with an EMF frequency of 10 Hz, at which the germination increased by 14%, reaching 94%. An increase in the EMF frequency to 16, 24, and 30 Hz led to an increase in germination by 9, 8, and 4%, respectively.The vitreousness of the wheat of the Shestopalivka variety treated with EMF after its storage for 9…13 months in 58% of the samples decreased by 1…17% compared to the control, and only in 29% of the samples it increased by 1…8%. However, 67% of all vitreous changes are below the margin of error. A steady decrease in grain vitreousness by 2...12% at a frequency of 10...30 Hz was noted, which persisted for about 12.5 months at a relative humidity of 33% and a temperature in the range of 9...23 °C. Significant increase in vitreousness was observed only in grain treated at a frequency of 16 Hz, after storage for 9.2 months at a relative humidity of 35% and a temperature of 23 °C. [ABSTRACT FROM AUTHOR]

Wheat grain yield is anticipated to suffer from the increased temperatures expected under climate change. In particular, the effects of post-anthesis temperatures on grain growth and development must be better understood in order to improve crop models. Grain growth and development involve several processes, and we hypothesized that some of the most important processes, namely grain dry biomass and water accumulation, grain volume expansion, and endosperm cell proliferation, will have different thermal sensitivity. To assess this, we established temperature–response curves of these processes for steady post-anthesis temperatures between 15 °C and 36 °C. From anthesis to maturity, grain dry mass, water mass, volume, and endosperm cell number were monitored, whilst considering grain temperature. Different sensitivities to heat of these various processes were revealed. The rate of grain dry biomass accumulation increased linearly up to 25 °C, while the reciprocal of its duration increased linearly up to at least 32 °C. In contrast, the growth rates of traits contributing to grain expansion, such as increase in grain volume and cell numbers, had higher optimum temperatures, while the reciprocal of their durations were significantly lower. These temperature–response curves can contribute to improve current crop models, and allow targeting of specific mechanisms for genetic and genomic studies. [ABSTRACT FROM AUTHOR]

Background: Mineral elements are important for maintaining good human health besides heavy metals. Mining genes that control mineral elements are paramount for improving their accumulation in the wheat grain. Although previous studies have reported some loci for beneficial trace elements, they have mainly focused on Zn and Fe content. However, little information is available regarding the genetic loci differences in dissecting synchronous accumulation of multiple mineral elements in wheat grains, including beneficial and heavy elements. Therefore, a genome-wide association study (GWAS) was conducted on 205 wheat accessions with 24,355 single nucleotide polymorphisms (SNPs) to identify important loci and candidate genes for controlling Ca, Fe, Zn, Se, Cu, Mn, Cd, As, and Pb accumulation in wheat grains. Results: A total of 101 marker-trait associations (MTAs) (P < 10–5) loci affecting the content of nine mineral elements was identified on chromosomes 1B, 1D, 2A, 2B, 3A, 3B, 3D, 4A, 4B, 5A, 5B, 5D, 6B, 7A, 7B, and 7D. Among these, 17 major MTAs loci for the nine mineral elements were located, and four MTAs loci (P < 10–5) were found on chromosomes 1B, 6B, 7B, and 7D. Eight multi-effect MTAs loci were detected that are responsible for the control of more than one trait, mainly distributed on chromosomes 3B, 7B, and 5A. Furthermore, sixteen candidate genes controlling Ca, Fe, Zn, Se, Cd, and Pb were predicted, whose functions were primarily related to ion binding, including metals, Fe, Ca, Cu, Mg, and Zn, ATP binding, ATPase activity, DNA binding, RNA binding, and protein kinase activity. Conclusions: Our study indicated the existence of gene interactions among mineral elements based on multi-effect MTAs loci and candidate genes. Meanwhile this study provided new insights into the genetic control of mineral element concentrations, and the important loci and genes identified may contribute to the rapid development of beneficial mineral elements and a reduced content of harmful heavy metals in wheat grain. [ABSTRACT FROM AUTHOR]

This paper investigates the activity concentration of radionuclides (226Ra, 232Th, 40K, and 137Cs) in the wheat grain samples using a high-purity germanium detector. Thirty-six wheat grain samples were collected from different locations of Koya City, Iraqi Kurdistan region. Average activity concentrations of 226Ra, 232Th, and 40K in wheat grain are found to be 0.407 ± 0.097 Bq.kg-1, 0.36 ± 0.14 Bq.kg-1, and 109.25 ± 2.214 Bq.kg-1 for 226Ra, 232Th, and 40K, respectively. The measured activity concentrations for the radionuclides are compared with the reported data from other countries. In addition, the fallout radionuclide of 137Cs has no detection of in the wheat grain samples. The radium equivalent activity Raeq, internal and external hazard indices Hin and Hex, and annual gonadal dose equivalent are calculated for the measured samples. The total ingestion dose is 113.19 µSv.y-1, which is below the world average value of 290 µSv.y-1. [ABSTRACT FROM AUTHOR]

SUMMARY: We expressed a bacterial glucan synthase (AgrobacteriumGlgA) in the cytosol of developing endosperm cells in wheat grains, to discover whether it could generate a glucan from cytosolic ADP‐glucose. Transgenic lines had high glucan synthase activity during grain filling, but did not accumulate glucan. Instead, grains accumulated very high concentrations of maltose. They had large volumes during development due to high water content, and very shrivelled grains at maturity. Starch synthesis was severely reduced. We propose that cytosolic glucan synthesized by the glucan synthase was immediately hydrolysed to maltose by cytosolic β‐amylase(s). Maltose accumulation resulted in a high osmotic potential in developing grain, drawing in excess water that stretched the seed coat and pericarp. Loss of water during grain maturation then led to shrinkage when the grains matured. Maltose accumulation is likely to account for the reduced starch synthesis in transgenic grains, through signalling and toxic effects. Using bioinformatics, we identify an isoform of β‐amylase likely to be responsible for maltose accumulation. Removal of this isoform through identification of TILLING mutants or genome editing, combined with co‐expression of heterologous glucan synthase and a glucan branching enzyme, may in future enable elevated yields of carbohydrate through simultaneous accumulation of starch and cytosolic glucan. Significance Statement: Grains of transgenic wheat expressing a bacterial glucan synthase in the endosperm cytosol did not accumulate cytosolic glucan but instead accumulated very high levels of maltose, presumably due to hydrolysis of nascent glucan by an endogenous β‐amylase. [ABSTRACT FROM AUTHOR]

Recent weather fluctuations imposing heat stress at the time of wheat grain filling cause frequent losses in grain yield and quality. Field-based studies for understanding the effect of terminal heat stress on wheat are complicated by the effect of multiple confounding variables. In the present study, the effect of day and day–night combined heat stresses during the grain-filling stage was studied using gene expression and proteomics approaches. The gene expression analysis was performed by using real-time quantitative PCR (RT-qPCR). The expression of genes related to the starch biosynthetic pathway, starch transporters, transcription factors, and stress-responsive and storage proteins, at four different grain developmental stages, indicated the involvement of multiple pathways. Under the controlled conditions, their expression was observed until 28 days after anthesis (DAA). However, under the day stress and day–night stress, the expression of genes was initiated earlier and was observed until 14 DAA and 7 DAA, respectively. The protein profiles generated using two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and matrix-assisted laser desorption/ionization time-of-flight mass spectroscopy (MALDI-TOF MS/MS) showed a differential expression of the proteins belonging to multiple pathways that included the upregulation of proteins related to the translation, gliadins, and low-molecular-weight (LMW) glutenins and the downregulation of proteins related to the glycolysis, photosynthesis, defense, and high-molecular-weight (HMW) glutenins. Overall, the defense response to the day heat stress caused early gene expression and day–night heat stress caused suppression of gene expression by activating multiple pathways, which ultimately led to the reduction in grain-filling duration, grain weight, yield, and processing quality. [ABSTRACT FROM AUTHOR]

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Spring wheat is produced in Kazakhstan and Western Siberia on a large area exceeding 16 mln ha. The crop grows during the short season from May till September in the extensive production system. The objective of the study was the evaluation of wheat grain for the concentration of macroelements Calcium (Ca), Potassium (K), Magnesium (Mg), Phosphorus (P), and Sulfur (S); toxic heavy metals Arsenium (As), Cadmium (Cd), Chromium (Cr), Lithium (Li), and Lead (Pb), and microelements essential for both plants and humans Copper (Cu), Iron (Fe), Manganese (Mn), Selenium (Se), and Zink (Zn). Samples were collected in 2017 and 2018 from across Kazakhstan and Omsk region. The analysis was conducted at Ionomics Facility at the University of Nottingham. The results demonstrated the high food safety of local grain. Despite the presence of the industry in Aktobe, East Kazakhstan, and Omsk the concentration of toxic metals (As, Cd, Cr, Li, and Pb) were below the limit of quantification or present in minor quantities not likely to affect human health. There was great spatial and yearly variation in the concentrations of macro- and microelements likely to be due to the soil properties, cropping systems, moisture, and other factors. The concentration of essential microelements was similar to wheat grain produced in other countries with exception to Zn. The concentrations of this important element exceeded 50 µg/g in Omsk and East Kazakhstan, above the values targeted by the Harvest Plus biofortification program. Even with the losses of Zn during milling, this grain will be beneficial for human health. [ABSTRACT FROM AUTHOR]

Background: Wheat spike architecture is a key determinant of multiple grain yield components and detailed examination of spike morphometric traits is beneficial to explain wheat grain yield and the effects of differing agronomy and genetics. However, quantification of spike morphometric traits has been very limited because it relies on time-consuming manual measurements. Results: In this study, using X-ray Computed Tomography imaging, we proposed a method to efficiently detect the 3D architecture of wheat spikes and component spikelets by clustering grains based on their Euclidean distance and relative positions. Morphometric characteristics of wheat spikelets and grains, e.g., number, size and spatial distribution along the spike can be determined. Two commercial wheat cultivars, one old, Maris Widgeon, and one modern, Siskin, were studied as examples. The average grain volume of Maris Widgeon and Siskin did not differ, but Siskin had more grains per spike and therefore greater total grain volume per spike. The spike length and spikelet number were not statistically different between the two cultivars. However, Siskin had a higher spikelet density (number of spikelets per unit spike length), with more grains and greater grain volume per spikelet than Maris Widgeon. Spatial distribution analysis revealed the number of grains, the average grain volume and the total grain volume of individual spikelets varied along the spike. Siskin had more grains and greater grain volumes per spikelet from spikelet 6, but not spikelet 1–5, compared with Maris Widgeon. The distribution of average grain volume along the spike was similar for the two wheat cultivars. Conclusion: The proposed method can efficiently extract spike, spikelet and grain morphometric traits of different wheat cultivars, which can contribute to a more detailed understanding of the sink of wheat grain yield. [ABSTRACT FROM AUTHOR]