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The effect of weak intercalated layers on the seismic response of rock slopes is investigated. Dozens of rock slopes containing weak intercalated layers are modeled in FLAC. The geometric property of the weak intercalated layer has a significant effect on the seismic slope response. The responses are generally greater as the thickness of the weak intercalated layer increases. The slope case with a 10-m-thickness and 20°-dip-angle weak intercalated layer yields the strongest dynamic response. A predictive model for the amplification of peak ground acceleration is developed, which can be used to estimate the acceleration amplifications for weak-layer-containing rock slopes. [ABSTRACT FROM AUTHOR]

Calcium (Ca) and magnesium (Mg) in drinking water and the relevant health effects have been ignored for too long. This study aims to reveal the concentrations, spatial distributions, origins and contributions to the daily estimated average requirements of Ca and Mg in public drinking water. Using hydrochemical data of collected samples of public drinking water in 314 cities across China, the contributions of Ca and Mg intakes from public drinking water to their daily estimated average requirements (EARs) were assessed. And the significance of Ca/Mg ratio and total hardness (TH) was evaluated as well. The Ca and Mg concentrations of the samples were in the range of 2.5–155.1 mg/L and 0.2–81.9 mg/L, with an average of 40.4 mg/L and 12.4 mg/L, respectively. There exist obvious differences in Ca and Mg concentrations in different regions, under the impact of climate conditions and water–rock interactions. The intake of Ca via the consumption of public drinking water for adults may be twice as much as that for other age groups. In cities with high Ca levels in public drinking water, the Ca contributions to EAR could reach up to 51.59% for adults. By contrast, Mg in drinking water is an important and even the main pathway to ingest Mg for infants and children. Therefore, public drinking water is critical for Ca and Mg intake among urban residents of China. Besides, attention should be paid to the health effects of high Ca/Mg ratio and low TH in public drinking water, especially in southern China. This research is the first systematic and comprehensive national scale study of Ca and Mg in public drinking water and can provide an important reference to improve healthy public drinking water standards around the world. [ABSTRACT FROM AUTHOR]

Myocardial fibrosis is a critical factor in the development of heart failure with preserved ejection fraction (HFpEF). Linggui Qihua decoction (LGQHD) is an experienced formula, which has been proven to be effective on HFpEF in clinical and in experiments. Objective. This study aimed to observe the effect of LGQHD on HFpEF and its underlying mechanism. Methods. Spontaneously hypertensive rats (SHR) were induced with high-glucose and high-fat to establish HFpEF models and were treated with LGQHD for 8 weeks. The heart structure was detected by echocardiography, and the histopathological changes of the myocardium were observed by hematoxylin-eosin (HE) and Masson staining. Reverse transcription PCR (RT-PCR) and western blot were used to detect mRNA and protein expression of the target gene in rat myocardium. Results. In this study, LGQHD improved cardiac morphology and atrial fibrosis in HfpEF rats, decreased tissue inhibitor of metalloproteinase-1 (TIMP-1) mRNA expression, up-regulated matrix metalloproteinase-9 (MMP-9) mRNA expression, and inhibited the expression of angiotensin II (Ang II), angiotensin II type 1 receptor (AT1), transforming growth factor β1 (TGF-β1), Smad2/3 mRNA, and protein in myocardial tissue of HFpEF rats. Conclusion. LGQHD can suppress atrial fibrosis in HFpEF by modulating the TGF-β1/Smad2/3 pathway. [ABSTRACT FROM AUTHOR]

Two polysaccharides from Carthamus tinctorius L. (CTLP-1 and CTLP-2) were purified, and their structures were analyzed by physical and chemical testing. CTLP-1 had a mass of 5900 Da that was composed of arabinose, glucose, and galactose with a mass molar ratio of 6.7:4.2:1. The backbone of CTLP-1 was →1)-α-GalAp-(1→4)-α-Arap-(1→2)-α-Glup-(4→. CTLP-2 had a mass of 8200 Da that was composed of arabinose, glucose, and galactose with a mass molar ratio of 16.76:4.28:1. The backbone of CTLP-2 was →1)-α-Galp-(2,6 →1)-α-Arap-(4,6 →1)-α-Glup-(3→. Both of them exhibited a high reducing power, hydroxyl radical scavenging activity, DPPH radical scavenging activity and ABTS radical scavenging activity, moderate Fe2+ chelating activity and superoxide anion scavenging activity, implying that they might be potential antioxidants. [ABSTRACT FROM AUTHOR]

Water resource is in high demand within the Yangtze River Delta, given its developed economy. Long-term exploitation of this resource has posed risks of artificial pollution and seawater intrusion to the shallow groundwater. This study aims to reveal the hydrochemical characteristics and health risks of shallow groundwater in the coastal plain of the Yangtze River Delta, as well as to discuss the possible factors affecting groundwater quality. Standard methods for hydrochemical parameter measurements, water quality assessment, and health risk models were applied to fulfill the objectives of the study. The results showed that the shallow groundwater was slightly alkaline, and the average values of total dissolved solids (TDS) and total hardness (TH) were 930.74 mg/L and 436.20 mg/L, respectively. The main hydrochemical types of groundwater were HCO3–Ca·Mg and HCO3–Ca·Na, accounting for 44.3% and 47.5%, respectively. In addition, As concentration was generally high, with a mean value of 0.0115 mg/L. The principal factors affecting the groundwater components include water-rock interactions (especially silicate), cation exchange, seawater intrusion, and human activities. The data also showed that As is strongly influenced by the redox of Fe, Mn, and NO3−. The results of the groundwater quality evaluation indicated that the shallow groundwater in some regions was unsuitable for drinking and agricultural irrigation. Health risk assessment showed that 44.3% of the water samples had significant health risks, which was attributed to the high As concentration. Therefore, it is urgent to establish long-term As monitoring to maintain sustainable groundwater management and drinking water safety. The results of this study provide essential data for water resource management and human health security in the Yangtze River Delta. [ABSTRACT FROM AUTHOR]

Objective: Current pharmacological intervention for the cancer-related pain is still limited. The aim of this study was to explore whether repetitive transcranial magnetic stimulation (rTMS) could be an effective adjuvant therapy to reduce pain in patients with advanced non-small cell lung cancer (NSCLC). Methods: This was a randomized, sham–controlled study. A total of 41 advanced NSCLC patients with uncontrolled pain (score≥4 on pain intensity assessed with an 11-point numeric rating scale) were randomized to receive active (10 Hz, 2000 stimuli) (n = 20) or sham rTMS (n = 20) for 3 weeks. Pain was the primary outcome and was assessed with the Numeric Rating Scale (NRS). Secondary outcomes were oral morphine equivalent (OME) daily dose, quality of life (WHO Quality of Life-BREF), and psychological distress (the Hospital Depression and Anxiety Scale). All outcomes were measured at baseline, 3 days, 1 week, 2 weeks, and 3 weeks. Results: The pain intensity in both groups decreased gradually from day 3 and decreased to the lowest at the week 3, with a decrease rate of 41.09% in the rTMS group and 23.23% in the sham group. The NRS score of the rTMS group was significantly lower than that of the sham group on the week 2 (p < 0.001, Cohen's d =1.135) and week 3 (p =0.017, Cohen's d = -0.822). The OME daily dose, physiology and psychology domains of WHOQOL-BREF scores, as well as the HAM-A and HAM-D scores all were significantly improved at week 3 in rTMS group. Conclusion: Advanced NSCL patients with cancer pain treated with rTMS showed better greater pain relief, lower dosage of opioid, and better mood states and quality of life. rTMS is expected to be a new effective adjuvant therapy for cancer pain in advanced NSCLC patients. [ABSTRACT FROM AUTHOR]

Stabilizing piles are commonly used to effectively reinforce landslides and unstable slopes. However, the reinforcement mechanism of stabilizing piles, especially portal double-row (PDR) piles, remains poorly understood. The deformation characteristics and stabilizing mechanism of PDR piles were examined through physical model tests and numerical modeling by considering various embedded depths and interbedded weak and hard bedrock combinations. First, physical model tests were performed to study the effects of interbedded bedrock, orthogonal joints in the upper hard stratum, and embedded depths on the stabilizing mechanism. Second, six groups of numerical models based on the physical models were developed using the distinct element method. The results show that the bending moment and horizontal displacement of PDR piles increase in the presence of orthogonal joints and decrease with increasing embedded depth. The effects of crossbeam and hard bedrock percentage on the PDR pile deformation mechanism were then assessed. The results show that the crossbeam significantly affects the stabilizing PDR pile mechanism, while the effect of hard bedrock percentage is relevant to the embedded depth of PDR piles. The study provides some theoretical information for landslide prevention in areas with interbedded weak and hard bedrock. [ABSTRACT FROM AUTHOR]

During anther development, the transformation of the microspore into mature pollen occurs under the protection of first the tetrad wall and later the pollen wall. Mutations in genes involved in this wall transition often lead to microspore rupture and male sterility; some such mutants, such as the reversible male sterile (rvms) mutant, are thermo/photoperiod-sensitive genic male sterile (P/TGMS) lines. Previous studies have shown that slow development is a general mechanism of P/TGMS fertility restoration. In this study, we identified restorer of rvms-2 (res2), which is an allele of QUARTET 3 (QRT3) encoding a polygalacturonase that shows delayed degradation of the tetrad pectin wall. We found that MS188, a tapetum-specific transcription factor essential for pollen wall formation, can activate QRT3 expression for pectin wall degradation, indicating a non-cell-autonomous pathway involved in the regulation of the cell wall transition. Further assays showed that a delay in degradation of the tetrad pectin wall is responsible for the fertility restoration of rvms and other P/TGMS lines, whereas early expression of QRT3 eliminates low temperature restoration of rvms-2 fertility. Taken together, these results suggest a likely cellular mechanism of fertility restoration in P/TGMS lines, that is, slow development during the cell wall transition of P/TGMS microspores may reduce the requirement for their wall protection and thus support their development into functional pollens, leading to restored fertility. This work identified a restorer (res2) of the P/TGMS mutant rvms-2 ; res2 is an allele of QRT3 , which encodes a polygalacturonase that participates in tetrad pectin wall degradation. The authors showed that a delay in degradation of the tetrad pectin wall is responsible for fertility restoration of rvms and other P/TGMS lines, revealing a likely cellular mechanism of fertility restoration in P/TGMS lines. [ABSTRACT FROM AUTHOR]

Rainfall represents one of the most significant cause of landslide hazards. With runoff in the saturated zone of shallow soil considered, an improved Green–Ampt model was proposed to depict the rainfall infiltration process. Furthermore, a formula for calculating the depth of wetting front was derived, and the slope stability was evaluated accordingly. Finally, a formula for calculating the safety factor of multilayer slopes above different surfaces was obtained via the limit equilibrium method. An illustration is a multilayer colluvial slope in Guangdong Province of China, the results indicated that the downward speed of wetting front decreases gradually within the same layer with continuous rainfall but increases significantly when it extends to the interfaces between the soil layers. Safety factor of the slope decreases sharply at the initial stage of rainfall but then more slowly. Slope failure is more likely to occur along the first interface between the soil layers than along the surface of wetting front. The result from the proposed model is closer to the result of the physical model test than from the existed Green–Ampt model. The improved Green–Ampt model for multilayer slopes can accurately evaluate the infiltration process and related stability of multilayer slopes under rainfall. [ABSTRACT FROM AUTHOR]

Due to the fact that strontium (Sr) is not involved in the scope of supervision of drinking water in China, the Sr concentration in public drinking water and its related health risks have been neglected for a long time. In this research, public drinking water samples were collected from 314 cities across the country to reveal the concentration and spatial distribution of Sr in public drinking water. In addition, the Monte Carlo method (a statistical simulation method) was applied to evaluate the Sr intake from drinking water and human health risks among different age groups and different regions. As shown in the results, the Sr was in the concentration range of 0.005–3.11 mg/L with a mean value of 0.360 mg/L. There were significant differences in the Sr concentration in different regions; in general, it was high in the north and low in the south. The Sr intakes of infants, children, teens, and adults from drinking water were 0.273, 0.503, 0.633, and 0.784 mg/day, respectively. There was a significant positive correlation between Sr concentration in drinking water and bone mineral density (BMD) in the elderly. Especially, the correlation coefficients (r) between Sr concentration and the BMD of the elderly whose age fell in the range of 60–70 years were 0.692 (male) and 0.483 (female). In addition, the Sr concentration in drinking water was positively correlated with the incidence of children's rickets (r = 0.411), while the Ca/Br ratio was negatively correlated with the incidence of children's rickets (r = − 0.410). According to the health risk assessment, among people of different ages, infants' hazard index (HI) value was the highest. The mean value and 95th percentile value were 0.066 and 0.247. Non-carcinogenic risk of Sr through drinking water among different people in different regions was less than 1, which meant no significant damage to human health. This study is the first time to systematically investigate Sr in public drinking water across the whole country. More importantly, the conclusions can be applied to risk control and management of public drinking water. [ABSTRACT FROM AUTHOR]

PANX2 forms large-pore channels mediating ATP release in response to physiological and pathological stimuli. Although PANX2 shows involvements in glioma genesis, the underlying mechanism remains unclear. PANX2 mRNA expression was analyzed via Oncomine and was confirmed via Gene Expression Profiling Interactive Analysis (GEPIA). The influence of PANX2 on overall survival (OS) of glioma was evaluated using LinkedOmics and further assessed through Cox regression analysis. The correlated genes with PANX2 acquired from LinkedOmics were validated through GEPIA and cBioPortal. Protein-protein interaction (PPI) of these genes was then obtained using Search Tool for the Retrieval of Interacting Genes (STRING) and Cytoscape with MCODE plug-in. All the PANX2-related genes underwent Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. The correlation between PANX2 and cancer immune infiltrates was evaluated via Tumor Immune Estimation Resource (TIMER). A higher expression of PANX2 only revealed a better OS in brain low grade glioma (LGG). PANX2-related genes in LGG functionally enriched in neuroactive ligand-receptor interaction, synaptic vesicle cycle, and calcium signaling. The hub genes from highest module of PPI were mainly linked to chemical synaptic transmission, plasma membrane, neuropeptide, and the pathway of neuroactive ligand-receptor interaction. Besides, PANX2 expression was negatively associated with infiltrating levels of macrophage, dendritic cells, and CD4+ T cells. This study demonstrated that PANX2 likely participated in LGG pathogenesis by affecting multiple molecular pathways and immune-related processes. PANX2 was associated with LGG prognosis and might become a promising therapeutic target of LGG. [ABSTRACT FROM AUTHOR]

Purpose: This study aimed to explore the key molecular pathways involved in Duchenne muscular dystrophy (DMD) and Becker muscular dystrophy (BMD) and thereby identify hub genes to be potentially used as novel biomarkers using a bioinformatics approach. Methods: Raw GSE109178 data were collected from the Gene Expression Omnibus (GEO) database. Weighted gene co-expression network analysis (WGCNA) was conducted on the top 50% of altered genes. The key modules associated with the clinical features of DMD and BMD were identified. Gene Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses were performed using the DAVID website. A protein-protein interaction (PPI) network was constructed using the STRING website. MCODE, together with the Cytohubba plug-ins of Cytoscape, screened out the potential hub genes, which were subsequently verified via receiver operating characteristic (ROC) curves in other datasets. Results: Among the 11 modules obtained, the black module was predominantly associated with pathology and DMD, whereas the light-green module was primarily related to age and BMD. Functional enrichment assessments indicated that the genes in the black module were primarily clustered in "immune response" and "phagosome," whereas the ones in the light-green module were chiefly enriched in "protein polyubiquitination". Eleven essential genes were eventually identified, including VCAM1, TYROBP, CD44, ITGB2, CSF1R, LCP2, C3AR1, CCL2, and ITGAM for DMD, along with UBA5 and UBR2 for BMD. Conclusion: Overall, our findings may be useful for investigating the mechanisms underlying DMD and BMD. In addition, the hub genes discovered might serve as novel molecular markers correlated with dystrophinopathies. [ABSTRACT FROM AUTHOR]

Duchenne muscular dystrophy (DMD) is one of the most severe neuromuscular disorders. Long non-coding RNAs (lncRNAs) are a group of non-coding transcripts, which could regulate messenger RNA (mRNA) by binding the mutual miRNAs, thus acting as competing endogenous RNAs (ceRNAs). So far, the role of lncRNA in DMD pathogenesis remains unclear. In the current study, expression profile from a total of 33 DMD patients and 12 healthy people were downloaded from Gene Expression Omnibus (GEO) database (GSE38417 and GSE109178). Differentially expressed (DE) lncRNAs were discovered and targeted mRNAs were predicted. The ceRNA network of lncRNAs—miRNAs—mRNAs was then constructed. Genome Ontology (GO) enrichment and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses of the putative mRNAs in the ceRNA network were performed through Database for Annotation, Visualization and Integration Discovery (DAVID) website. Topological property of the network was analyzed using Cytoscape to disclose the hub lncRNAs. According to our assessments, 19 common DElncRNAs and 846 common DEmRNAs were identified in DMD compared to controls. The created ceRNA network contained 6 lncRNA nodes, 69 mRNA nodes, 27 miRNA nodes and 102 edges, while four hub lncRNAs (XIST, AL132709, LINC00310, ALDH1L1-AS2) were uncovered. In conclusion, our latest bioinformatic analysis demonstrated that lncRNA is likely involved in DMD. This work highlights the importance of lncRNA and provides new insights for exploring the molecular mechanism of DMD. The created ceRNA network contained 6 lncRNA nodes, 69 mRNA nodes, 27 miRNA nodes and 102 edges, while four hub lncRNAs (XIST, AL132709, LINC00310, ALDH1L1-AS2) were uncovered. Remarkably, KEGG analysis indicated that targeted mRNAs in the network were mainly enriched in "microRNAs in cancer" and "proteoglycans in cancer". Our study may offer novel perspectives on the pathogenesis of DMD from the point of lncRNAs. This work might be also conducive for exploring the molecular mechanism of increased incidence of tumorigenesis reported in DMD patients and experimental models. [ABSTRACT FROM AUTHOR]

Sporophytic pollen coat proteins (sPCPs) derived from the anther tapetum are deposited into pollen wall cavities and function in pollen–stigma interactions, pollen hydration, and environmental protection. In Arabidopsis, 13 highly abundant proteins have been identified in pollen coat, including seven major glycine-rich proteins GRP14, 16, 17, 18, 19, 20, and GRP–oleosin; two caleosin-related family proteins (AT1G23240 and AT1G23250); three lipase proteins EXL4, EXL5 and EXL6, and ATA27/BGLU20. Here, we show that GRP14, 17, 18, 19, and EXL4 and EXL6 fused with green fluorescent protein (GFP) are translated in the tapetum and then accumulate in the anther locule following tapetum degeneration. The expression of these sPCPs is dependent on two essential tapetum transcription factors, MALE STERILE188 (MS188) and MALE STERILITY 1 (MS1). The majority of sPCP genes are up-regulated within 30 h after MS1 induction and could be restored by MS1 expression driven by the MS188 promoter in ms188 , indicating that MS1 is sufficient to activate their expression; however, additional MS1 downstream factors appear to be required for high-level sPCP expression. Our ChIP, in vivo transactivation assay, and EMSA data indicate that MS188 directly activates MS1. Together, these results reveal a regulatory cascade whereby outer pollen wall formation is regulated by MS188 followed by synthesis of sPCPs controlled by MS1. [ABSTRACT FROM AUTHOR]

Pollen wall consists of several complex layers which form elaborate species-specific patterns. In Arabidopsis, the transcription factor ABORTED MICROSPORE (AMS) is a master regulator of exine formation, and another transcription factor, TRANSPOSABLE ELEMENT SILENCING VIA AT-HOOK (TEK), specifies formation of the nexine layer. However, knowledge regarding the temporal regulatory roles of TEK in pollen wall development is limited. Here, TEK-GFP driven by the AMS promoter was prematurely expressed in the tapetal nuclei, leading to complete male sterility in the pAMS:TEK-GFP (pat) transgenic lines with the wild-type background. Cytological observations in the pat anthers showed impaired callose synthesis and aberrant exine patterning. CALLOSE SYNTHASE5 (CalS5) is required for callose synthesis, and expression of CalS5 in pat plants was significantly reduced. We demonstrated that TEK negatively regulates CalS5 expression after the tetrad stage in wild-type anthers and further discovered that premature TEK-GFP in pat directly represses CalS5 expression through histone modification. Our findings show that TEK flexibly mediates its different functions via different temporal regulation, revealing that the temporal regulation of TEK is essential for exine patterning. Moreover, the result that the repression of CalS5 by TEK after the tetrad stage coincides with the timing of callose wall dissolution suggests that tapetum utilizes temporal regulation of genes to stop callose wall synthesis, which, together with the activation of callase activity, achieves microspore release and pollen wall patterning. Author summary: To develop into mature pollen grains, microspores require formation of the pollen wall. To date, pollen wall developmental events, including production and transportation of pollen wall components, synthesis and degradation of the callose wall, and deposition and demixing of primexine, have been studied in Arabidopsis, and a number of anther- or tapetum-specific genes involved in pollen wall formation have been uncovered. However, whether the specific expression patterns of these genes contribute to pollen wall development or patterning remains unclear. Here, we show that TEK, a transcription factor that specifies formation of nexine (the inner layer of the pollen wall exine), represses the expression of the callose synthase CalS5 after the tetrad stage, which accurately fits with the timing of callose wall dissolution causing microspore release. Moreover, we show that premature expression of TEK in the wild-type anthers disturbs callose wall synthesis and pollen wall patterning. This work reveals that a pollen wall regulator must be kept under a strict temporal control to perform its functions, and that these temporal controls are coordinated with other pollen wall developmental events to determine pollen wall formation and patterning. [ABSTRACT FROM AUTHOR]

Natural rock often suffers from cyclic wetting–drying involving different water types, and the resulting deterioration may differ from laboratory tests using distilled water or salt solutions. An inappropriate estimation of this deterioration effect may lead to fatal geological hazards and engineering failures. A multiscale study is conducted to investigate the physical and mechanical features of sandstone in Three Gorges Reservoir region (TGR sandstone) subjected to cyclic wetting–drying of Yangtze River water. During this study, three types of water, i.e., Yangtze River water, ionized water having similar ion compositions as the Yangtze River water, and distilled water, are used for comparison. The results show that the multiscale physical properties including mineral compositions (especially calcite and albite), micro-pore parameters, computed tomography values, and macro-mechanical parameters (i.e., Young's modulus, uniaxial compression strength and tensile strength) are remarkably altered during the cyclic wetting–drying process. Significant correlations are found between these numerous multiscale properties. The results indicate that changes of mineral compositions and microstructure are the primary reasons for the deterioration of sandstone strength. The deterioration effect of distilled water on TGR sandstone is the least, while the effect of ionized water is the greatest, and that of river water being intermediate. These differences are ascribed to different chemical interactions, together with possible microorganism effects for river water, as microorganisms in river water potentially weaken the deterioration of cyclic wetting–drying of river water. In situ water is recommended for studying how rock properties are affected by water–rock interactions in real settings. [ABSTRACT FROM AUTHOR]

Poly(lactic acid) (PLA) ultrafiltration membranes modified by montmorillonite (MMT) nanoparticles were fabricated via phase inversion method. The influences of MMT nanoparticles on the structure and performances of the membranes were investigated by water contact angle measurement, porosity and mean pore size measurement, scanning electron microscopy (SEM) analysis, atomic force microscopy (AFM) analysis, thermogravimetric analysis (TGA), filtration and fouling experiments. The results revealed that the hydrophilicity of the composite membrane surface was improved significantly, and the porosity and mean pore size increased after MMT nanoparticles were added. When the mass fraction of MMT nanoparticle was 0.2%, the surface of the composite membrane surface was smoother than that of pure membrane, the size of finger-like cavities increased, the thermal stability increased, the pure water flux reached the maximum value with 450 L/(m² ⋅ h), the flux recovery ratio reached up to 95.1 % and the antifouling property was the best. While excessive dosage of MMT nanoparticles will significantly increase the viscosity of casting solution, and make the aggregation of nanoparticles, resulting in the poor performance of the PLA membrane. [ABSTRACT FROM AUTHOR]

The sexine layer of pollen grain is mainly composed of sporopollenins. The sporophytic secretory tapetum is required for the biosynthesis of sporopollenin. Although several enzymes involved in sporopollenin biosynthesis have been reported, the regulatory mechanism of these enzymes in tapetal layer remains elusive. ABORTED MICROSPORES ( AMS) and MALE STERILE 188/ MYB103/ MYB80 ( MS188/ MYB103/ MYB80) are two tapetal cell-specific transcription factors required for pollen wall formation. AMS functions upstream of MS188. Here we report that AMS and MS188 target the CYP703A2 gene, which is involved in sporopollenin biosynthesis. We found that AMS and MS188 were localized in tapetum while CYP703A2 was localized in both tapetum and locule. Chromatin immunoprecipitation (Ch IP) showed that MS188 directly bound to the promoter of CYP703A2 and luciferase-inducible assay showed that MS188 activated the expression of CYP703A2. Yeast two-hybrid and electrophoretic mobility shift assays ( EMSAs) further demonstrated that MS188 complexed with AMS. The expression of CYP703A2 could be partially restored by the elevated levels of MS188 in the ams mutant. Therefore, our data reveal that MS188 coordinates with AMS to activate CYP703A2 in sporopollenin biosynthesis of plant tapetum. [ABSTRACT FROM AUTHOR]

This study aimed at determining the effects of purified chondroitin sulfate (ChS) from pig laryngeal cartilage on the lipid and antioxidant metabolisms of male Sprague-Dawley rats that are fed a high-fat diet. Thirty-two male rats were divided into four groups and fed for 5 weeks on a standard diet, a high-fat diet or a high-fat diet plus ChS. It was demonstrated that the high-fat diet provoked lipid peroxidation and induced a severe depletion of lipoprotein lipase, hepatic lipidase, glutathione, catalase and superoxide dismutase levels. ChS was effective in reducing triglycerides, total cholesterol, low-density-lipid cholesterol and malondialdehyde levels elevated by the high-fat diet. In addition, ChS might reduce the risk of atherosclerosis, as high-density-lipid cholesterol, lipoprotein lipase, hepatic lipidase and the ratio of high-density-lipoprotein cholesterol to total cholesterol were significantly higher than in the high-fat-diet rats. ChS restored the endogenous antioxidants glutathione, catalase and superoxide dismutase. These results showed that ChS was potent in lipid-lowering and altering the antioxidative enzyme; however, excess ChS would disturb lipid profiles that went beyond the normal limits. [ABSTRACT FROM AUTHOR]

This study compared the free radical-scavenging properties of chondroitin sulfate (ChS) from pig laryngeal cartilage and its reduced or sulfonated derivatives. The binding behavior between Cu 2+ and ChS and its derivatives, and the interaction between superoxide radical and ChS were studied by fluorescence quenching, equilibrium dialysis, infrared spectra and thermal analysis. Purified ChS inhibited the generation of hydroxyl radical and scavenged superoxide radical in a concentration-dependent manner. Reduced ChS did not scavenge hydroxyl radical and superoxide radical. Sulfonated ChS had no hydroxyl radical scavenging activity but scavenged superoxide radical as strongly as purified ChS. ChS showed strong binding activity with Cu 2+ in deionized water but not in 0.01-M HCl. Both reduced ChS and sulfonated ChS did not exhibit such chelating behavior. The structural basis of hydroxyl radical inhibiting of ChS was attributed to a complex of the Cu 2+ with the carboxyl group of glucuronic acid residue. The reaction of superoxide radical with the sulfate ester and the carboxyl group may be the basis of superoxide radical scavenging activity of ChS. [ABSTRACT FROM AUTHOR]

Plant growth and mineral element accumulation in Brassica juncea var. crispifolia (crisped-leaf mustard) under exposure to lanthanum (La) and cadmium (Cd) were studied by employing a hydroponic experiment with a complete two-factorial design. Four levels of La (0.05–5.0 mg L -1 ) and two levels of Cd (1.0 and 10.0 mg L -1 ) were used in this experiment. Lanthanum did not improve plant growth in this experiment. Addition of La (≥ 1.0 mg L -1 ) or Cd (≥ 10 mg L -1 ) to the solution inhibited root elongation. Lanthanum treatments reduced accumulations of iron (Fe), manganese (Mn), and zinc (Zn) in roots, and Mn in shoots. Lanthanum at ≥ 1.0 mg L -1 limited the Cd translocation from roots to shoots and thus decreased the accumulation of Cd in shoots. Cadmium had no influence on La accumulations in roots, but inhibited the accumulation of La in shoots. The study results suggest that applications of rare earth elements in vegetables would be potentially risky to human health. [ABSTRACT FROM AUTHOR]