Search

Current practice of wastewater treatment does not recover the full potential of energy present in wastewater. The potential of using anammox bacteria for autotrophic nitrogen removal combined with a desire for energy optimization brings new attention to the A-stage technology for organic carbon harvesting from municipal wastewater. The goal of this research was to investigate operational conditions of four full-scale A-stage processes and gain insight in the optimal conditions to harvest the maximum amount of organics present in sewage as excess sludge from the A stage. Large differences in removal efficiencies and design aspects were found between the four operational A-stage processes in the Netherlands. Biochemical oxygen demand (BOD) removal efficiencies vary between 40% and 80%, indicating that a good removal efficiency is possible, but that local conditions or design can be very influential. An optimal solid retention time (SRT) for maximal sludge production of 0.3 days was found; a longer SRT resulted in more mineralization of the chemical oxygen demand (COD). SRT control might be an important design aspect for the optimization of A-stage process. A short contact time with a minimum of 15 min and sufficient aeration were found to be optimal for soluble COD removal. Iron addition aided the removal of colloidal/suspended COD by coagulation/flocculation. Sludge flocs formed in the A-stage process are weak and sensitive to anaerobic conditions as well as shear due to, for example, pumping. Besides a good design of the Astage itself, the further processing of the produced sludge also needs careful attention to optimize the sludge production and energy production. [ABSTRACT FROM AUTHOR]

Background: In this research, the feasibility of, and population dynamics in, one-step anaerobic sequencing batch reactor systems treating the fine sieved fraction (FSH) from raw municipal wastewater was studied under thermophilic (55 °C) and mesophilic (35 °C) conditions. FSH was sequestered from raw municipal wastewater, in the Netherlands, using a rotating belt filter (mesh size 350 micron). FSH is a heterogeneous substrate that mainly consists of fibres originating from toilet paper and thus contains a high cellulosic fraction (60-80 % of total solids content), regarded as an energy-rich material. Results: Results of the 656-day fed-batch operation clearly showed that thermophilic digestion was more stable, applying high organic loading rates (OLR) up to 22 kg COD/(m3 day). In contrast, the mesophilic digester already failed applying an OLR of 5.5 kg COD/(m3 day), indicated by a drop in pH and increase in volatile fatty acids (VFAs). The observed viscosity values of the mesophilic sludge were more than tenfold higher than the thermophilic sludge. 454-pyrosequencing of eight mesophilic and eight thermophilic biomass samples revealed that Bacteroides and aceticlastic methanogen Methanosaeta were the dominant genera in the mesophilic digester, whereas OP9 lineages, Clostridium and the hydrogenotrophic methanogen Methanothermobacter dominated the thermophilic one. Conclusions: Our study suggests that applying thermophilic conditions for FSH digestion would result in a higher biogas production rate and/or a smaller required reactor volume, comparing to mesophilic conditions. [ABSTRACT FROM AUTHOR]

Extremophilic organisms require specialized enzymes for their exotic metabolisms. Acid-loving thermophilic Archaea that live in the mudpots of volcanic solfataras obtain their energy from reduced sulphur compounds such as hydrogen sulphide (H2S) and carbon disulphide (CS2). The oxidation of these compounds into sulphuric acid creates the extremely acidic environment that characterizes solfataras. The hyperthermophilic Acidianus strain A1-3, which was isolated from the fumarolic, ancient sauna building at the Solfatara volcano (Naples, Italy), was shown to rapidly convert CS2 into H2S and carbon dioxide (CO2), but nothing has been known about the modes of action and the evolution of the enzyme(s) involved. Here we describe the structure, the proposed mechanism and evolution of a CS2 hydrolase from Acidianus A1-3. The enzyme monomer displays a typical ?-carbonic anhydrase fold and active site, yet CO2 is not one of its substrates. Owing to large carboxy- and amino-terminal arms, an unusual hexadecameric catenane oligomer has evolved. This structure results in the blocking of the entrance to the active site that is found in canonical ?-carbonic anhydrases and the formation of a single 15-Å-long, highly hydrophobic tunnel that functions as a specificity filter. The tunnel determines the enzyme's substrate specificity for CS2, which is hydrophobic. The transposon sequences that surround the gene encoding this CS2 hydrolase point to horizontal gene transfer as a mechanism for its acquisition during evolution. Our results show how the ancient ?-carbonic anhydrase, which is central to global carbon metabolism, was transformed by divergent evolution into a crucial enzyme in CS2 metabolism. [ABSTRACT FROM AUTHOR]

In biofilm environments, heavy metal and radionuclide pollutants are removed by a variety of mechanisms, including biosorption, precipitation as sulfides or phosphates and microbial reductive precipitation. Even if the elemental composition and localization of the precipitate trapped in the biofilm is well described thanks to spectroscopic and microscopic techniques, this review highlights that little is known about metal immobilisation mechanisms in microbial biofilms, i.e., mass transfer of metals, mechanisms involved in (bio)sorption and precipitation and the influence of physicochemical micro-environments within the biofilm matrix. The review shows the advantage of using a combination of different techniques to evaluate the fate of metals within microbial biofilms. By combining a variety of techniques (e.g., selective extraction, microscopy, spectroscopy and miniaturised sensors …), it is possible to gain high-resolution structural and chemical information of biofilms on a level of the individual cell. This approach will facilitate the characterization of the metal immobilisation sites and the metal sorption and (bio)crystallisation mechanisms in biofilms. The results provided by the combination of these techniques will allow to predict the amount of metal accumulation in biofilms as well as their chemical speciation. This review demonstrates that an interdisciplinary approach is required to study metal fate within the biofilm matrix. [ABSTRACT FROM AUTHOR]