1. Esculentoside A exerts anti-oxidative stress and anti-apoptotic effects in rat experimental membranous nephropathy by regulating MAPK pathway.
- Author
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Wu, Jin, Lei, Genping, Wang, Ting, Dong, Sheng, and Zhan, Xiaolin
- Abstract
Background: Membranous nephropathy is characterized by deposition of immune complex. The initial inflammatory responses induce cell apoptosis and oxidative injury, thus contributing to the pathogenesis of chronic kidney disease. Esculentoside A exerts anti-inflammatory, anti-apoptotic and anti-oxidant abilities, and protects against acute kidney injury. Objective: The renoprotective effect of Esculentoside A on a rat model with membranous nephropathy was investigated in this study. Results: Injection of cationic bovine serum albumin promoted the level of 24 h proteinuria, and induced histopathological damage, including glomerular atrophy and thick glomerular basement membrane in the kidney tissues. Intraperitoneal injection with Esculentoside A reduced the level of 24 h proteinuria, and attenuated the pathological damages. Esculentoside A attenuated cationic bovine serum albumin-induced reduction of Bcl-2 protein expression, enhancements in the protein expressions of Bax and cleaved caspase-3. Besides, the enhanced levels of MDA (malondialdehyde) and NO (nitric oxide), and reduced levels of SOD (superoxide dismutase) and GSH (glutathione), in rats with membranous nephropathy were restored by Esculentoside A injection. Moreover, Esculentoside A counteracted with the promotive effects of cationic bovine serum albumin on the protein expressions of phosphorylated JNK (p-JNK), p-ERK1/2 and p-p38. Conclusion: The renoprotective effects of Esculentoside A on a rat model with membranous nephropathy was mediated by its anti-oxidant and anti-apoptotic effects through inactivation of MAPKs pathway. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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