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Nano-fertilizers with higher efficacy compared to conventional fertilizers can provide advantage for plant cultivation in both productive and problematic soils. Therefore, this study aimed to determine the effect of nano-calcium (nano-Ca) on lettuce plants grown in saline-boron toxic soil. Nano-calcium fertilizer was prepared from eggshells. Functional and structural properties of nano-Ca was determined by scanning electron microscopy (SEM), x-ray diffraction (XRD) and fourier transform infrared spectroscopy (FTIR) before plant experiment. The treatments was; control, 40 mM NaCl and 20 mg kg−1 B (NaCl + B), and 40 mM NaCl and 20 mg kg−1 B with 4 mM Nano-Ca (NaCl + B + nano-Ca). The nano-Ca significantly increased the dry weight and calcium (Ca) concentration of lettuce plants under saline-B toxic conditions. Although there was a decrease in the concentrations of sodium (Na), chloride (Cl), and boron (B) with nano-Ca treatment, it was not statistically significant. Salinity and boron toxicity lead to increased lipid peroxidation. In the present study, the production of malondialdehyde (MDA) as a marker for lipid peroxidation, along with a significant decrease in hydrogen peroxide (H2O2) concentration, was observed with the application of nano-Ca. There was no significant alteration in superoxide dismutase activity (SOD) observed in lettuce grown under saline and boron toxic conditions. However, catalase activity (CAT) increased with nano-Ca application, while the activity of ascorbate peroxidase (APX) decreased. The study results suggest that nano-Ca serves a protective function for lettuce plants cultivated under saline and boron toxic conditions. [ABSTRACT FROM AUTHOR]

The study aimed to explore how phosphoric acid-modified rice husk biochar (PBC) affects the growth and concentrations of essential and non-essential elements in two crops: lettuce as the primary crop and arugula as the secondary crop. The treatments consisted of a no P fertilized control and 250 mg P kg−1 sourced either from PBC, triple super phosphate (TSP) or phosphoric acid (PA). When subjected to phosphorus (P) sources, both lettuce and arugula exhibited a noteworthy rise in their dry weights compared to the control plants. PBC treatments significantly increased P concentration in both plants. Although the PBC treatment decreased lettuce nitrogen (N) concentration, it had no impact on arugula N concentration. Phosphorus treatments resulted in a decrease in lettuce K concentration, whereas it increased in arugula. Calcium (Ca), magnesium (Mg) and sulfur (S) concentrations in both plants were not affected by P sources. The zinc (Zn) concentrations of the plants notably decreased with P treatments. Moreover, P treatments led to a reduction in manganese (Mn) concentration specifically in arugula. PBC significantly increased the silicon (Si) concentrations of lettuce plants. The application of PA significantly increased the vanadium (V) concentration in arugula. Phosphoric acid treatments resulted in an increase in the plant bromine (Br) concentrations. To conclude, PBC may serve as an alternative P source, potentially being as effective as or even more effective than other P sources in influencing plant nutrition, without causing an undesirable elevation of non-essential elements in plants. [ABSTRACT FROM AUTHOR]

TWO FIELD experiments were done in order to reduce the effect of water stress on head lettuce, therefore this study were conducted to impact the influence of vermicompost soil addition and foliar application of proline and melatonin on growth (fresh, dry weights, leaf area, relative water content, numbers of outer leaves, in addition chlorophyll a, b, and carotene contents), yield (head height, head diameter, head weight and total yield), and quality (total dissolved solids (TDS), total carbohydrates, crude protein, total sugar, crude fiber percentages, vitamin C, vitamin E, nitrate (NO3), nitrite (NO2), proline, super oxidase dismutase, peroxidase and catalyse) of head lettuce (lactuca sativa) cv Kharga under water stress (100, 75 and 50% of full irrigation). The results showed the combined treatment of vermicompost and foliar spray with proline under 75% of full irrigation provided the highest vegetative growth, photosynthetic pigments, head yield and quality relative to other treatments including plants grown full irrigation without vermicompost and foliar spray with tap water treatments. These results suggest that yield and quality of head lettuce could be improved under abiotic stress including water scarcity through application of proline, melatonin and vermicompost, additionally these treatments could save water quantities. [ABSTRACT FROM AUTHOR]

The nutritive mineral element concentrations of most African leafy vegetables (ALVs) are influenced by the plant’s growth stages. Momordica balsamina is an ALV in the Cucurbitaceae family, rich with nutraceutical and pharmaceutical properties, available in the plant at different growth stages. A greenhouse study was conducted to determine the responses of the nutritional value of M. balsamina leafy vegetable harvested at different growth stages. Six growth stages, namely, vegetative (reference), bud development, flower initiation, fruit set, fruit development and physiological maturity, served as treatments, with 10 replicates and arranged in RCBD. Treatments had highly significant (P ≤ 0.01) effect on the tested nutritional quality, namely, potassium (K), calcium (Ca), magnesium (Mg), zinc (Zn), iron (Fe) and phosphorus (P). Relative to the reference, K was the highest (1206 mg/L) at flower initiation stage, whereas the highest Ca (127.70 mg/L) and Zn (2.21 mg/L) concentrations occurred at the fruit development stage. At the physiological maturity stage, the highest Mg (25.19 mg/L), P (16.54 mg/L) and Fe (5.49 mg/L) concentrations, were recorded. Noticeably, during early growth stages, P, Fe and Zn were negatively reduced, when compared to K, Ca and Mg, which were increasing at every growth stages. In conclusion, the greatest accumulation of the tested mineral elements was mainly observed as from when the leafy vegetable started setting fruit until physiological maturity stage. [ABSTRACT FROM AUTHOR]

Vegetable cultivation using eco-enzyme application is one way to improve its production. The study aimed to evaluate the growth response of pak choi using eco-enzyme in several concentrations and its interval. The experiment used a randomized complete block design with two factors (concentrations and interval of eco-enzyme applications). The results showed that the application of eco-enzyme with a concentration of 30 mL L-1 indicated an optimal growth stimulator in pak choi, but it was not significantly different as compared to 50 mL L-1. The application of eco-enzyme twice a week was able to stimulate plant growth including plant height, number of leaves, leaf area, shoot fresh weight, and root fresh weight. Furthermore, there was a linear correlation between leaf area and plant height, leaf number, shoot fresh weight, and root fresh weight that ranged from weak to strong levels. It is recommended to apply eco-enzyme at a concentration of 30 mL L-1 twice a week for optimal pak choi production. [ABSTRACT FROM AUTHOR]

Spinach (Spinacia oleracea) vegetables are an important constituent of the diet of rural communities worldwide. It is a suitable substitute for several indigenous leafy vegetables, as it has more or less the same texture after cooking. Consequently, growers tend to grow spinach in any soil type or mixture available to them without modifying it, leading to reduced growth performance and yields. The use of animal manure is common in South Africa and helps to improve soil fertility when used in the correct ratio mixture. Therefore, the study’s objective was to investigate which of the growing media mixture (GMM) ratios of sand, Hutton soil, and goat manure would improve the growth and yield of S. oleracea under greenhouse conditions. The experiment was laid out in a randomized complete block design (RCBD), with 10 replicates (n=60). Six treatments comprised of different types of GMM namely, sand, Hutton soil, and goat manure at T1 (1:3:0 -control), T2 (1:2:1), T3 (1:1:1), T4 (1:1:2), T5 (2:1:2) and T6 (2:3:2), respectively. Growing media mixture had significant (P ≤ 0.05) effect on plant height (PH), number of leaves (NL), chlorophyll content (CC) and root length (RL) of S. oleracea, contributing 78, 62, 76 and 54% of the total treatment variation (TTV) in the respective variables. Relative to the control (T1), treatment T2, T3, T4, T5 and T6 increased PH by 22.75 cm, 24.03 cm, 27.92 cm, 22.84 cm and 24.35 cm, respectively. Similarly, the NL in all the treatments T2, T3, T4, T5 and T6 was increased by 10.90, 11.70, 11.90, 11.70 and 11.60, respectively, compared to T1. A related trend was observed in the CC, treatments T2, T3, T4, T5 and T6, increased the CC by 14.99, 15.08, 26.45, 21.38 and 15.83, respectively when compared to T1. The GMM treatment T4 obtained the highest 27.92, 11.9, 26.45 and 42.38 in PH, NL, CC and RL. In conclusion, GMM T4 had a positive effect on the growth and yield of S. oleraceae, and it is recommended for use to grow the vegetable crop. [ABSTRACT FROM AUTHOR]

Productive traits, total polyphenols (TPC), and antioxidant activity (DPPH) of arugula submitted to the combination (or not) of cow urine and doses of bokashi were evaluated in two experimental areas. Arugula was planted in cultivation bags with 55 dm3 of capacity in protected cultivation. The treatments were bokashi doses (0, 10, 20, and 30 g) and use (or not) of cow urine diluted 1% in water. The variables evaluated were fresh leaf biomass (FLB), dry leaf biomass (DLM), plant height (PH), chlorophyll index, TPC, and DPPH. In area 1, all agronomic variables were increased at 30 g and 20 g bokashi doses. FLB was increased by 87 and 76% with 30 g of bokashi. Cow urine only increased PH. In area 2, the use of bokashi + cow urine increased FLB, DLB, and PH with a positive quadratic response. At the maximum point, the FLB was increased by 159% with 28.92 g of bokashi. Bokashi increased FLB and DLB in the two areas in all evaluated doses. For TPC, with the use of cow urine, 10 g of bokashi increased TPC by 14%. Without the use of cow urine, increases of 17 and 33% with 10 and 30 g of bokashi were observed. The 30 g of bokashi is recommended because of increased productive traits and TPC. [ABSTRACT FROM AUTHOR]

Amaranth is an annual or short-perennial, dicotyledonous plant, globally cultivated for its grains and leaves. The plant is recognized as one of the earliest domesticated vegetables with exceptional nutraceutical and therapeutic properties. The present investigation aims to evaluate the genetic diversity and breeding potential of 22 vegetable amaranthus genotypes. The experiment was conducted in randomized block design with three replications during summer 2023, with observations recorded on 14 agro-morphological traits. The analysis of variance revealed significant differences for all the traits, depicting a wide range of variation for these traits. Further, the genotype VRAM-45 was found most promising for leaf yield, while VRAM-44 excelled for seed yield. A significant and positive correlation exists between leaf yield/plant, leaf length and leaf width, implying a potential link between leaf area and the efficiency of photosynthesis. Principal component analysis highlighted the significance of the first two components, explaining a substantial 53.71% of the total variation. Traits such as plant height at the vegetative stage, leaf length, and days to 50% flowering emerged as significant contributors to the variance. Clustering analysis categorized the 22 genotypes into four clusters. Cluster IV, though was found monogenotypic, but found superior for most of the economic traits, followed by cluster III. Thus, focusing on these clusters in breeding or selection programs could lead to more favorable outcomes in terms of economic productivity and other desirable characteristics. [ABSTRACT FROM AUTHOR]

Tomatoes are the major ingredient in food preparation, which leads to a huge food production rate. Most countries cultivate huge tomatoes at the same time that crop diseases affect the production rate due to many different types of diseases. The various types of diseases are bacterial spots, septoria leaf spot, left mold, late blight, early blight, arget and spot. Many research studies review these tomato leaf diseases with various statistics. The survey on disease will give a clear idea of reasons and prevention methods, also presenting how to reduce it in the early stages. In another study, tomato leaf images were taken to classify the diseased and non-diseased varieties. Few studies compare the standard model of disease prediction with the machine learning models. Therefore, this research study discusses tomato leaf disease detection and prevention methods used by various researchers in their studies and finally consolidate the observations. This study also deals with encouraging hygiene permanence in tomato leaf using machine learning algorithms. The convolutional neural network (CNN) was used to predict the early nature of the hygiene nature of leafy vegetable plants for the benefit of agriculture people and concluded with better future suggestions. [ABSTRACT FROM AUTHOR]

Ensuring the technical and technological possibility of regularly obtaining fresh, high-quality plant production in Antarctic stations is an urgent task of our time. This work is devoted to studying the growth and development of leaf vegetable crops and the main quality indicators of their edible parts when grown in the phytotechnical complex greenhouses at the "Vostok" Antarctic station and at the agrobiopolygon of the Agrophysical Research Institute (AFI). The plants, belonging to 13 varieties of 9 types of leaf vegetable crops (arugula, garden cress, cabbage, mustard, leaf radish, leaf lettuce, amaranth, dill, parsley leaf), were studied during five growing seasons at the "Vostok" station and at the AFI agrobiopolygon under controlled conditions (control). The experimental data obtained demonstrate the high productivity of the phytotechnical complex for most of the investigated crops per unit of useful area, with lower costs of electricity and water consumption per unit of production compared with a number of greenhouses at foreign Antarctic stations and greenhouse complexes with controlled conditions located on other continents. Lettuce crops were the most adapted to the growing conditions at the Antarctic station "Vostok". They did not differ in their evaluated characteristics from the control. All other investigated crops, while not differing in their development rate and quality, had statistically significant (16–61%) decreases in their yield per 1 m2 per year. This may demonstrate the difference in the "genotype–environment" interaction in plants grown at the Antarctic station and AFI agrobiopolygon, probably due to the different barometric pressure and partial pressure of oxygen at the two locations. The positive psychological effects of the greenhouses were identified along with nutritional and other qualities of the plants. [ABSTRACT FROM AUTHOR]

Copyright of Journal of Agricultural Faculty of Gaziosmanpasa University / Gazi Osman Pasa Üniversitesi Ziraat Fakültesi Dergisi is the property of Gaziosmanpasa University, Agricultural Faculty and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Green synthesis of NPs is preferred due to its eco-friendly procedures and non-toxic end products. However, unintentional release of NPs can lead to environmental pollution affecting living organisms including plants. NPs accumulation in soil can affect the agricultural sustainability and crop production. In this context, we report the morphological and biochemical response of spinach nanoprimed with MgO–NPs at concentrations, 10, 50, 100, and 150 µg/ml. Nanopriming reduced the spinach root length by 14–26%, as a result a reduction of 20–74% in the length of spinach shoots was observed. The decreased spinach shoot length inhibited the chlorophyll accumulation by 21–55%, thus reducing the accumulation of carbohydrates and yield by 46 and 49%, respectively. The reduced utilization of the total absorbed light further enhanced ROS generation and oxidative stress by 32%, thus significantly altering their antioxidant system. Additionally, a significant variation in the accumulation of flavonoid pathway downstream metabolites myricitin, rutin, kaempferol-3 glycoside, and quercitin was also revealed on MgO-NPs nanopriming. Additionally, NPs enhanced the protein levels of spinach probably as an osmoprotectant to regulate the oxidative stress. However, increased protein precipitable tannins and enhanced oxidative stress reduced the protein digestibility and solubility. Overall, MgO-NPs mediated oxidative stress negatively affected the growth, development, and yield of spinach in fields in a concentration dependent manner. [ABSTRACT FROM AUTHOR]

The main purpose of applying organic or inorganic amendments is to guarantee crop safe production in heavy metal contaminated soil. However, previous studies showed that the effects of organic or inorganic composite amendments on the cadmium (Cd) concentration of lettuce (Lactuca sativa var. ramosa Hort) were inconsistent. Accordingly, a sixty-day pot experiment was carried out to examine the impacts of the inorganic materials (lime, L and zeolite, Z), organic materials (biochar, B and compost, C), and their combination on the immobilization of Cd in soil and its uptake by lettuce. The objective was to identify the most suitable soil amendment combination that promotes safe lettuce production. The results revealed that the combined application of BC, LZC, and LBC significantly increased the plant height by 11.09–28.04% and fresh weight by 183.47–207.67%. This improvement can be attributed to enhanced soil quality, such as increased dissolved organic carbon (DOC) by 70.19–80.42%, soil respiration (SR) by 29.04–38.46%, and soil microbial carbon content (SMC) by 36.94–46.63%. Compared to inorganic fertilizers and their combination with organic amendments, organic amendments had a significant impact on reducing shoot Cd concentration by 33.93%-56.55%, while increasing the activity of catalase by 138.87–186.86%. And soil available Cd measured by diffusive gradients in thin-films (DGT-Cd) decreased 24.73–88.13% in all treatments. Correlation analysis showed that plant Cd concentration was significantly correlated with soil pH, SR, cation exchange capacity (CEC), DOC and SMC. These results demonstrated that organic amendments, especially the combination of biochar and compost, have greater potential than inorganic amendments and inorganic–organic combinations for realizing safe production of lettuce and improving soil quality in the Cd moderately contaminated acid farmland. [ABSTRACT FROM AUTHOR]

Growing population in Malaysia has resulted in increased production of local vegetables as well as pesticide usage. This constitutes a health risk to human health. In the present work, the level of ten pesticide residues namely chlorpyrifos, profenofos, aldrin, endrin, cypermethrin, lambda-cyhalothrin, carbendazim, propamocarb, imidacloprid, and thiamethoxam in ten types of vegetables collected from six local markets were measured using modified QuEChERS (quick, easy, cheap, effective, rugged, and safe) coupled with gas chromatography-tandem mass spectrometry (GC-MS/MS) and ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). Results showed that 13.3% samples contained pesticide residues above the maximum residue limit (MRL) prescribed by the Malaysian Food Regulations 1985, 55.0% of samples contained pesticide residues below the MRL, and no pesticide residues were detected in 31.7% of samples. Carbendazim and chlorpyrifos were among the highest pesticides detected in the samples. For the type of vegetables, kale and spinach contained high concentrations of pesticide residues above the MRL. In order to produce safe vegetables, the efficiency of different washing methods (tap water, 10% sodium bicarbonate solution, and 10% acetic acid solution) in reducing carbendazim and chlorpyrifos residues in a kale model system was evaluated. Results showed that the levels of carbendazim and chlorpyrifos reduction for all three methods were significantly different (p < 0.05) with 10% acetic acid solution being the most effective followed by 10% sodium bicarbonate solution, and tap water. Washing kale with 10% acetic acid reduced 76.0 and 41.2% of carbendazim and chlorpyrifos, respectively. Therefore, it is recommended for consumers to practice 10% sodium bicarbonate washing method by soaking vegetables with an acidic solution followed by rinsing with tap water to reduce pesticide residues, and minimise the exposure to hazardous pesticides. [ABSTRACT FROM AUTHOR]

1. Introduction: Basil (Ocimum basilicum L.), as an annual plant, belongs to the family Lamiaceae, native to Asia (Iran, India, Pakistan, Thailand, etc.) and grows in warm and semi-warm regions. Arsenic (As) is a highly dangerous heavy metal for plants and human. This quasi-metal is widely distributed in the earth's crust, and also is the twentieth most abundant element in the earth's crust. Selenium (Se) is a non-metallic chemical element and also has similarities to arsenic. Global interest in the biological effects of selenium on the environment and the food chain is growing because selenium is essential as a micronutrient for many organisms, including humans and other animals (although it is toxic in high concentrations). Given that arsenic is one of the most important factors limiting crop production in the agricultural sector, it seems necessary to conduct researches in this field to eliminate or reduce the toxic effects of these element on agricultural plants. 2. Materials and Methods: In this study, the effect of selenium on increasing the tolerance rate of basil plant against arsenic heavy metal stress was investigated as a factorial experiment based on completely randomized design under greenhouse condition. The first factor included arsenic-contaminated soil (4576 mg kg-1) and non-contaminated soil, and the second factor included different concentrations of selenium treatment (0, 5 and 10 mg L-1 selenium sodium). Foliar application of selenium was conducted at two stages. The first stage of foliar application was carried out at 4-leaf stage and the second stage of foliar application was done two weeks after the first stage. Leaf number, leaf area, stem height, fresh and dry weight of leaf and stem, chlorophyll and carotenoid content, free proline content, phenol, flavonoid and anthocyanin content, electrolyte leakage and antioxidant enzymes activity were evaluated at the end of the experiment. 3. Results and Discussion: The results showed that arsenic stress negatively affected the morphological traits such as leaf number and area, fresh and dry weight of leaf, fresh and dry weight of stem and plant height, but selenium treatment alleviated the toxicity of arsenic and increased the values of these traits, so that treated plants had higher values of morphological characteristics comparing to control plants under arsenic heavy metal stress. The highest values of morphological traits were observed in plants treated with the highest concentration of selenium (10 mg L-1). In terms of physiological traits, arsenic heavy metal stress reduced the values of some of these traits, so that the lowest amounts of chlorophyll a, chlorophyll b and total chlorophyll, anthocyanin, phenol, and flavonoids were recorded in plants grown in arsenic-contaminated soil without foliar application of selenium. On the other hand, the lowest activity rates of guaiacol peroxidase, catalase and ascorbate peroxidase were observed in plants grown in noncontaminated soil and without selenium foliar application. Also, the both arsenic stress and selenium treatment increased proline accumulation and soluble sugars content of treated plants comparing to control plants. Heavy metal stress increases the production and accumulation of reactive oxygen species (ROS) and damages the membrane structure resulting in more electrolyte leakage. Selenium application led to increasing plant resistance rate against arsenic stress, mainly by enhancing cells antioxidant capacity and osmotic potential. 4. Conclusion: Considering basil is one of the most important aromatic vegetables which has fresh consumption, crispness and wateriness of its leaves and young shoots is one of the most important commercially traits which directly affect crop marketability. Considering arsenic stress has a negative effect on leaf morphological traits (number, area, fresh weight and dry weight) and stem characteristics (fresh weight, dry weight and height), it is recommended to use selenium foliar application as an efficient technique to overcome adverse effects of arsenic stress on basil production in regions with contaminated soils. [ABSTRACT FROM AUTHOR]

Copyright of Argentinian Horticulture / Horticultura Argentina is the property of Revista Horticultura Argentina and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Red and blue light-emitting diodes (LEDs) affect the quality of sweet potato leaves and their nutritional profile. Vines cultivated under blue LEDs had higher soluble protein contents, total phenolic compounds, flavonoids, and total antioxidant activity. Conversely, chlorophyll, soluble sugar, protein, and vitamin C contents were higher in leaves grown under red LEDs. Red and blue light increased the accumulation of 77 and 18 metabolites, respectively. Alpha-linoleic and linolenic acid metabolism were the most significantly enriched pathways based on Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analyses. A total of 615 genes were differentially expressed between sweet potato leaves exposed to red and blue LEDs. Among these, 510 differentially expressed genes were upregulated in leaves grown under blue light compared with those grown under red light, while the remaining 105 genes were expressed at higher levels in the latter than in the former. Among the KEGG enrichment pathways, blue light significantly induced anthocyanin and carotenoid biosynthesis structural genes. This study provides a scientific reference basis for using light to alter metabolites to improve the quality of edible sweet potato leaves. [ABSTRACT FROM AUTHOR]

Intensive vegetable crop systems are rapidly developing, with consequences for greenhouse gas (GHGs) emissions, nitrogen leaching and soil carbon. We undertook a field trial to explore the effect of biochar application (0, 10, 20 and 40 t ha−1) on these factors in lettuce, water spinach and ice plant rotation. Our results show that the 20 and 40 t ha−1 soil treatments resulted in the SOC content being 26.3% and 29.8% higher than the control (0 t ha−1), respectively, with significant differences among all treatments (p <.05). Biochar application caused N2O emissions to decrease during the lettuce and water spinach seasons, by 1.5%–33.6% and 12.4%–40.5%, respectively, compared the control, with the 20 t ha−1 application rate resulting in the lowest N2O emissions. Biochar also decreased the dissolved nitrogen (DN) concentration in leachate by 9.8%–36.2%, following a 7.3%–19.9% reduction in dissolved nitrogen in the soil. Similarly, biochar decreased the nitrate (NO3−) concentrations in leachate by 3.9%–30.2%, following a 3.8%–16.7% reduction in the soil nitrate level. Overall, straw biochar applied at rate of 20 t ha−1 produced the lowest N2O emissions and N leaching, while, increasing soil carbon. [ABSTRACT FROM AUTHOR]

Red amaranth (Amaranthus cruentus L.) is a fast-growing and nutritious leafy vegetable. The seed population density needs to be known appropriately to obtain the optimal yield of marketable sizes plants. The residue of black soldier fly (BSF) larvae culture has been recognized as a potential source of nutrients for cultivating red spinach plants. This study was designed to search for the best combination of plant population density and BSF concentration to obtain the optimal marketable yield of the red amaranth plant. In addition, an accurate leaf area estimation of the red amaranth plant was also developed using leaf dimensions as a predictor with several regression models. Two experiments were conducted. Firstly, the BSF residue was applied at a high rate (up to 50%) using a floating culture system. Application of the residue at the rate of 30% and higher significantly inhibited the growth of the red amaranth. Secondly, the application rate was reduced to 10% and 20%, and the experiment was conducted using the conventional cultivation system. Results indicated that the optimum application rate of the BSF residue was 10% for enhancing growth. Application of seed density at 30 mg/dm² produced an optimum marketable yield of the red amaranth since a higher population density causes plant-plant competition. Thus, creating high size variability within the population or reducing the average size of the harvested plants. Lastly, the leaf area of red amaranth can be accurately estimated using the leaf length x width (LW) as a predictor using the zero-intercept linear regression model. [ABSTRACT FROM AUTHOR]

Quinoa is a multi-purpose vegetable, grain, and forage crop, due in part to the high nutritional value of its aerial parts. This work evaluates quinoa genotype characteristics as a starting point for a better understanding of multi-purpose cultivation. Ten cultivars of quinoa were studied on soddy-podzolic soils: Brightest Brilliant, Red Faro, and Cherry Vanilla from the US (USA 1–3); Titicaca (KY1) from Denmark; Regalo (KY2), a cultivar selected by the Baer Seed Research Center for southern Chile; as well as Q1–Q5, UAE cultivars of various ecological and geographical origins. Quinoa plants were divided into three parts (lower, middle, and upper). The Q3 and Q4 cultivars produced the maximum fresh weight (38.7 g and 35.4 g, respectively) and dry matter (5.6 g and 5.3 g, respectively). The leaf mass and stems comprised 25% and 75% of the lower parts, versus 50–60% and 40–50% of the middle parts, respectively. Stems made up about 15% of the upper parts. The KY1 and Q5 cultivars produced the highest results (4.08 and 4.23 g, respectively). Protein concentrations of the quinoa grains were relatively high, with up to 14.0% grain protein in the USA2 cultivars. Leucine and isoleucine were the most abundant amino acids in quinoa grains, ranging from 6.7 to 9.2 g/100 g of protein. In contrast, methionine was the least abundant amino acid with less than 1.5 g/100 g of protein. [ABSTRACT FROM AUTHOR]

Copyright of Iraqi Journal of Science is the property of Republic of Iraq Ministry of Higher Education & Scientific Research (MOHESR) and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Objective This study appraises the nutraceutical potential of uncooked and cooked Ceylon spinach, the wild leafy vegetable Talinum triangulare occurring in southwest India. Materials and Methods Proximal properties (moisture, crude protein, total lipids, crude fiber, ash, total carbohydrates, and calorific value), mineral contents, amino acid composition, in vitro protein digestibility (IVPD), protein digestibility–corrected amino acid score (PDCAAS), protein efficiency ratios (PERs), and fatty acid methyl esters (FAMEs) were evaluated by following standard protocols. Results Total lipids, crude fiber, total carbohydrates, and calorific value were higher in cooked than in uncooked samples. Among nine minerals assessed, sodium, magnesium, phosphorous, iron, manganese, and zinc were significantly higher in uncooked samples compared with cooked samples. Lysine, methionine, cystine, tyrosine, alanine, arginine, aspartic acid, glutamic acid, and serine were higher and histidine, isoleucine, leucine, phenylalanine, valine, glycine, and proline were lower in uncooked samples. Cooking improved IVPD, PDCAAS, and PER. Capric and linoleic acids were higher in uncooked samples, while palmitic, 1-pyrrolidinebutanoic, and ɑ-linolenic acid acids were higher in cooked samples. The total unsaturated fatty acids (TUFAs) were higher in cooked samples; however, the total saturated fatty acids (TSFAs) were lower in cooked samples. The ratio of TUFA to TSFA was higher in uncooked than in cooked samples. Conclusion The protein content of T. triangulare is comparable to those of legume seeds and consists of low fat, high fiber, and high carbohydrates along with increased calorific value. The Na/K (<1) and Ca/P (>1) ratios are favorable to combat blood pressure and prevention of calcium loss, respectively. The indispensable amino acids are comparable to or higher than soybean, wheat, and Food and Agriculture Organization of the United Nations and the World Health Organization (FAO-WHO) standards. Improved IVPD, PDCAAS, and PER in cooked samples of T. triangulare support its nutraceutical potential in human diet to tackle the protein-energy malnutrition and supplement of its flour strengthens the value of fortified foods. The nutraceutical potential has possible roles to combat lifestyle diseases such as obesity, diabetes, cancers, and cardiac ailments. [ABSTRACT FROM AUTHOR]

Copyright of Cahiers Agriculture is the property of EDP Sciences and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Agricultural Sciences, Belgrade is the property of University of Belgrade, Faculty of Agriculture and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

This study aimed to compare cormels and suckers to determine the better planting material (PM) and to evaluate the effects of morphology-altering treatments on both shoot growth and corm yield in taro plants. The PMs used included cormel (PMC), suckers with two leaves (PMS2) and suckers with four leaves (PMS4). Morphological alteration (MA) treatment included removal of all suckers (MAS), excising the mother plant (MAM), and non-treated control (NMA). Data were collected weekly for non-destructive and at 12, 20, and 28 weeks after planting (WAP) for destructive measurements. Results of this study indicated that the SPAD values were higher in taro plants grown using cormel and morphologically non-altered plants. Removal of all suckers caused the mother plant to increase the number of leaves. The dry weight of the leaf blades, petioles and fibrous roots of the mother plant and the number of suckers decreased after the corm enlargement process took place. Fresh and dry weights of the corm increased at 20 WAP and then slowed down at 28 WAP. The moisture content of corm was relatively constant at 75%. Cormel could form on the fibrous roots and on suckers, but the total wet and dry weights of the cormels were decreased if the suckers were periodically removed. The growth of suckers was very dominant compared to the NMA plant when the mother plant was excised. Meanwhile, if all the suckers were removed, the growth of the mother plant was relatively comparable to that of the NMA plants. [ABSTRACT FROM AUTHOR]

Ceiba pentandra (L.) Gaertn, is a leafy vegetable that is under-utilized due to lack of information about the nutritional potential and agronomic practices. Minerals, vitamins, and protein content of succulent leaves of C. pentandra plants grown under different fertilizer rates (Control (No fertilizer), 5 t ha-1 of poultry manure (PM) + 200 kg ha-1 of NPK, 10 t ha-1 of PM, 20 t ha-1 of PM, 450 kg ha-1 of NPK, and 20 t ha-1 of PM + 100 kg ha-1 of NPK) in the Department of Crop Science Garden, University of Nigeria, Nsukka were evaluated using standard analytical procedures in a replicated trial. Analysis of variance indicated a non-significant effect of fertilizer rates on vitamin contents assessed. Protein and the minerals evaluated showed non-significant response to fertilizer except zinc and calcium. Highest concentration of zinc (0.46 mg/100g) was attributed to 20 t ha-1 of PM which was statistically similar to 0.43 and 0.42 mg/100g obtained from leaves of plants grown without fertilizer and 5 t ha-1 of PM + 200 kg ha-1 of NPK, respectively. Soil amendment at 5 t ha-1 of PM + 200 kg ha-1 of NPK resulted in high accumulation of calcium (145.00 mg/100g). Conclusively, application of 20 t ha-1 of PM or 5 t ha-1 of PM + 200 kg ha-1 of NPK may be appropriate for growing C. pentandra. The study established that C. pentandra can be brought under regular cultivation culture without compromising the nutritional quality. [ABSTRACT FROM AUTHOR]

Leafy vegetables are used in various cuisines worldwide; however, as they cannot be peeled and their leaf surface area is large, the risk of retaining pesticide residues on these vegetables is relatively higher than on others. To our knowledge, this is the first comparative study to reveal the effect of removing pesticide residues from five artificially contaminated leafy vegetables (lettuce, perilla leaves, spinach, crown daisy, and ssamchoo (Brassica lee ssp. namai)) using different removal methods. The percent reduction range for each method was 43.7–77.0%, and the reduction range for the five leafy vegetables was 40.6–67.4%. Lettuce had the highest reduction (67.4 ± 7.3%), whereas ssamchoo had the lowest reduction (40.6 ± 12.9%). Spinach and crown daisy showed no significant difference in their reductions. Based on reduction by method, running water (77.0 ± 18.0%) and boiling (59.5 ± 31.2%) led to the highest reduction, whereas detergent (43.7 ± 14.5%) led to the lowest reduction. The reductions of chlorfenapyr, diniconazole, indoxacarb, fludioxonil, pyraclostrobin, and lufenuron in the leafy vegetables were lower with blanching and boiling than with other methods (p < 0.05). These results highlight the importance of thoroughly washing leafy vegetables to lower the intake of pesticide residues before cooking. [ABSTRACT FROM AUTHOR]

Lettuce is one of the most consumed leafy greens. Depending on the variety, it is ready for harvesting 40–80 days after sowing, and therefore several growth cycles can be concluded within a growing season. Due to its high market standards, lettuce may require intensive management. This study implemented a critical period of weed interference (CPWI) trial to understand at which moment of the cropping cycle weeds can be tolerated without impacting crop yield to decrease the time needed for weeding and assess the potential support weeds can give to biological pest control in lettuce. Treatments represented two gradients of weed intensity: (1) increasingly weed-free, and (2) increasingly weedy. Dose–response curves were produced to find the CPWI based on lettuce relative yield. RLQ analysis was used to explore the relationships between weeding regime and weed functional traits for biological pest control. Yield was above the 5% acceptable yield loss threshold in all plots kept weed-free for 20 days or more, indicating a necessary weed-free period of 20 days from transplanting. However, the support of beneficial insects was not guaranteed at the end of the necessary weed-free period. We suggest that it is possible to limit intense weed management to the beginning of the growing season, reducing the cost of plastic mulches and increasing on-farm biodiversity, but field margins could be better suited to deliver conservation biological control in short-term crops where this service is of primary interest. [ABSTRACT FROM AUTHOR]

The ability of natural zeolite amendment to reduce the uptake of potentially toxic elements (PTEs) by lettuce, spinach and parsley was evaluated using pot experiments. PTE concentrations in roots and shoots, as well as the pseudo total (PT), water soluble (WS) and bioavailable (BA) PTE fractions in the amended soils, were assessed. Although the PT PTE concentration was high, the WS fraction was very low (<0.4%), while the BA fraction varied widely (<5% for Cr, Mn and Co, <15% for Ni, Pb and Zn, >20% for Cd and Cu). PTE concentration decreased in both roots and shoots of all leafy vegetables grown on zeolite amended soils, especially at high amendment dose (10%). The uptake of PTEs mainly depended on plant species, PTE type and amendment dose. With the exception of Zn in spinach, the bioaccumulation factor for roots was higher than for shoots. Generally, lettuce displayed the highest PTE bioaccumulation capacity, followed by spinach and parsley. Except for Zn in spinach, the transfer factors were below 1 for all PTEs, all plant species and all amendment doses. Our results showed that the natural zeolites are promising candidates in the reclamation of contaminated soils due to their ability to immobilize PTEs. [ABSTRACT FROM AUTHOR]

Copyright of Shipin Kexue/ Food Science is the property of Food Science Editorial Department and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Copyright of Journal of Agro-Environment Science is the property of Journal of Agro-Environment Science Editorial Board and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Microwave technology has wide applications, including extraction of active compounds in biomass and compost for agricultural use. A study was carried out to determine the effects of microwave power level from 0 (control) to 1000 W on the properties and active microbial groups in vermicast, and how it may impact the photosynthesis, plant growth, and yield of kale (Brassica oleracea var. sabellica) 'Red Russian'. Heat accumulation in the vermicast increased rapidly to a peak of 86 °C at 400 W before declining to 68 °C at 1000 W. Vermicast water loss increased exponentially up to 800 W before declining. The C:N ratio of the vermicast was reduced at ≥600 W while the pH remained the same. In a 2D-principal component analysis biplot, vermicast treated at 600, 800 and 1000 W were associated with Gram-positive (G+), GGram-negative (G−), G + G− bacteria, protozoa, and fungi groups while the 0, 200, and 400 W treated vermicast were associated with eukaryotes. However, the trend for total microbial mass was 200 W = 400 W > 0 W > 600 W = 800 W = 1000 W. Kale leaf anthocyanin, chlorophylls, and carotenoids were significantly (p = 0.001) increased by the 400 W or 600 W treatment compared to the other treatments. Stomatal conductance, transpiration, and photosynthesis rates were increased by the 400 W followed by the 600 W. As a result, yield of kale grown in the 400 W microwaved vermicast was the highest. Future studies will explain the functions of specific microbial populations and elemental composition in microwaved vermicast. [ABSTRACT FROM AUTHOR]

Leafy sweet potato (Ipomoea batatas L.) is an excellent source of nutritious greens and natural antioxidants, but reports on antioxidants content and activity at buds, leaves, petioles, and stems are scarce. Therefore, the total phenolics content (TPC), total anthocyanins content (TAC), and antioxidant activity (assessed by DPPH and ABTS radical scavenging activities and ferric reducing antioxidant power (FRAP)) were investigated in four aerial parts of 11 leafy sweet potato varieties. The results showed that varieties with pure green aerial parts, independently of the part analyzed, had higher TPC, FRAP, and ABTS radical scavenging activities. The green-purple varieties had a significantly higher TAC, while variety GS-17-22 had the highest TAC in apical buds and leaves, and variety Ziyang in petioles and stems. Among all parts, apical buds presented the highest TPC and antioxidant capacity, followed by leaves, petioles, and stems, while the highest TAC level was detected in leaves. The TPC was positively correlated with ABTS radical scavenging activity and FRAP in all parts studied, whereas the TAC was negatively correlated with DPPH radical scavenging activity. Collectively, the apical buds and leaves of sweet potato had the higher levels of nutritional values. These results would provide reference values for further breeding of leafy sweet potatoes. [ABSTRACT FROM AUTHOR]

Copyright of BIOAGRO is the property of Revista BIOAGRO and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)

Fertilizer originated nitrate excess in vegetables has attracted numerous studies for its effects on food quality. However, the relationship between plant nitrate accumulation and fertilizer-derived nitrogen (FTN) in the soil in continuous research is rarely reported. This study examines the impact of conventional ammonium fertilizer application (50.4 kg/ ha) on the constant trend of soil nitrogen and plant nitrate uptake of 8 common leafy vegetables grown in Red Delta River, Vietnam. The vegetables reveal both FTN and plant nitrate took about 17 days to release from topsoil and plant. The trend of FTN is well fitted by a regression model (Decay model, R2=0.945, p<0.001), which shows the nitrogen loss rate of FTN range from 0.120-0.139 g N/day. Meanwhile, the trend of plant nitrate uptake fitted the quadratic equation (R2=0.889, p<0.01). Although the correlation between FTN and plant nitrate is weak, this study finds that autumn crops have a tighter relationship than summer crops (R=0.71 and R=0.46, respectively). It can be concluded that regression models could be suitable methods to observe the behavior of fertilizer nitrogen in soil and vegetable uptake. [ABSTRACT FROM AUTHOR]

Leafy vegetables cultivated in kitchen gardens and suburban areas often accumulate excessive amounts of heavy metals and pose a threat to human health. For this reason, plenty of studies have focused on low accumulation variety screening. However, identifying specific leafy vegetable varieties according to the foliar uptake of air pollution remains to be explored (despite foliar uptake being an important pathway for heavy-metal accumulation). Therefore, in this study, the lead (Pb) and cadmium (Cd) contents, leaf morphology, and particle matter contents were analyzed in a micro-area experiment using 20 common vegetables. The results show that the Pb content in leaves ranged from 0.70 to 3.86 mg kg−1, and the Cd content ranged from 0.21 to 0.99 mg kg−1. Atmospheric particles were clearly scattered on the leaf surface, and the particles were smaller than the stomata. Considering the Pb and Cd contents in the leaves and roots, stomata width-to-length ratio, leaf area size, enrichment factor, and translocation factor, Yidianhongxiancai, Qingxiancai, Baiyuanyexiancai, Nanjingjiangengbai and Sijixiaobaicai were recommended for planting in kitchen gardens and suburban areas as they have low accumulation characteristics. Identifying the influencing factors in the accumulation of heavy metals in vegetables through foliar uptake is important to help plant physiologists/environmentalists/policy makers to select suitable varieties for planting in air-polluted areas and thus reduce their threat to human health. [ABSTRACT FROM AUTHOR]

Cadmium (Cd) contamination in agricultural soil is a global issue of concern because of its potential transfer to the human body through food chain interactions. Phosphate fertilizers used in the agricultural soils contain a high concentration of Cd. Composts are good sources of phosphorus, thus mixing of compost with phosphate fertilizer could reduce the total phosphate fertilizer use and subsequent Cd enrichment in agricultural soils. However, the optimum mixing ratio of compost and phosphate fertilizer is of paramount importance to obtain the desired outcome. In this study, experimental pot trials were performed to assess plant growth and Cd accumulation in two leafy vegetables, red amaranth (Amaranthus cruentus) and water spinach (Ipomoea aquatica), grown in Cd-contaminated loamy sandy soil under varying rates of compost and phosphate fertilizer application, either alone or in mixture. The study revealed that application of compost in combination with phosphate fertilizer is more effective from both environmental and economic perspectives rather than the application of compost alone, wherein 10 t/ha compost in combination with 40 kg/ha phosphate fertilizer was found as the best treatment for reducing Cd phytoaccumulation in both vegetable species and increasing the plant growth. Moreover, this optimum mixing ratio of compost and phosphate fertilizer showed positive impacts on soil pH and EC. The results also observed that phytoaccumulation of Cd decreases with decreasing the rate of phosphate fertilizer application, either alone or in mixture. Moreover, further study is necessary to determine the impact of combined treatments on microorganisms influencing Cd dynamics in soils. [ABSTRACT FROM AUTHOR]

The suppression effect of white light-emitting diode (LED) on spindly growth observed under monochromatic light of the red LED in watercress (Nasturtium officinale R. Br.) was investigated for production in plant factories. Watercress was cultured in a growth chamber under total photosynthetic photon flux density (PPFD) of 100 μmol•m-2•s-1 using three differential treatments; the ratio of red LED to white LED based on PPFD was 100:0 (R100W0), 90:10 (R90W10), and 75:25 (R75W25). Watercress was harvested 33 days after sowing. The longest internode length was observed with R100W0. Watercress in R100W0 were observed with the lowest value for node number, maximum leaf length, shoot fresh weight, shoot dry weight, dry matter rate, and SPAD. R90W10 recorded higher values for maximum leaf length, shoot fresh weight, shoot dry weight, dry matter rate, and SPAD than R100W0. In watercress cultivation, replacing 10% of the red LED with a white LED proved effective in inducing morphological changes and enhancing dry matter production. [ABSTRACT FROM AUTHOR]

Green leaf vegetables are an important component of the human diet, providing fibre, minerals and vitamins and are low in calories. They are also a very good source of antioxidants. In the present study an attempt was made to investigate the nutritional benefit of the common leafy vegetables used in South Indian cuisine such as Boerrhavia diffusa and Cucurbita maxima. Proximate analysis, an index to the nutritive value of foods and food ingredients was performed by standard procedures. It involves the determination of percentage of moisture content, crude lipids, crude protein, crude fibers and total ash present in the dried powdered leaves. Dietary minerals, trace elements and heavy metals were estimated by ICP-MS. The results showed that the high level of proximate content in both leaves. Mineral analysis showed that the B.diffusa contains the high level of Ca (6438.71 ppm) and C. maxima demonstrated the high level of Na (26.61 ppm), K(6143.51ppm) and Mg(610,62ppm). The overall study demonstrated that the leaves of both B. diffusa and C. maxima are having high proximate content, vitamin C, permissible limits of dietary minerals and concluded that these leafy vegetables consumed are nutritive and safe. [ABSTRACT FROM AUTHOR]

Background: Water supply limits agricultural productivity of many crops including lettuce. Identifying cultivars within crop species that can maintain productivity with reduced water supply is a significant challenge, but central to developing resilient crops for future water-limited climates. We investigated traits known to be related to water-use efficiency (WUE) and yield in lettuce, a globally important leafy salad crop, in a recombinant inbred line (RIL) lettuce mapping population, produced from a cross between the cultivated Lactuca sativa L. cv. Salinas and its wild progenitor L. serriola L. Results: Wild and cultivated lettuce differed in their WUE and we observed transgressive segregation in yield and water-use traits in the RILs. Quantitative trait loci (QTL) analysis identified genomic regions controlling these traits under well-watered and droughted conditions. QTL were detected for carbon isotope discrimination, transpiration, stomatal conductance, leaf temperature and yield, controlling 4–23 % of the phenotypic variation. A QTL hotspot was identified on chromosome 8 that controlled carbon isotope discrimination, stomatal conductance and yield under drought. Several promising candidate genes in this region were associated with WUE, including aquaporins, late embryogenesis abundant proteins, an abscisic acid-responsive element binding protein and glutathione S-transferases involved in redox homeostasis following drought stress were also identified. Conclusions: For the first time, we have characterised the genetic basis of WUE of lettuce, a commercially important and water demanding crop. We have identified promising candidate genomic regions determining WUE and yield under well-watered and water-limiting conditions, providing important pre-breeding data for future lettuce selection and breeding where water productivity will be a key target. [ABSTRACT FROM AUTHOR]

An Investigation on "Evaluation of underutilized leafy vegetables for yield and nutritional value" was conducted at Horticultural College and Research Institute, Periyakulam, TNAU during 2018-2019 to determine the performance of underutilized leafy vegetables for growth, foliage yield and their nutritional content. Fifteen underutilized green vegetables used by the native people were collected and studied for their development, yield, and nutritional parameters. The results showed significant variations for different characters. The highest fresh foliage yield was registered in the Alternanthera bettzickiana. Ascorbic acid content was highest in Acalypha indica with lower fiber content. Carotenoid content and sodium content were recorded maximum in Hibiscus cannabinus. Total phenolic content was detected highest in G13 Sauropus androgynus. Potassium and copper content was showed a maximum in Cardiospermum halicacabu. Magnesium and manganese content was noticed highest in Talinum fruticosum. Zinc and calcium content was registered maximum in G15 Solanum trilobatum. Iron content was observed maximum in Alternanthera sessilis [ABSTRACT FROM AUTHOR]

Lutein is a carotenoid that is selectively taken up into the macula of the eye. Green leafy vegetables, such as spinach and kale, are known to be excellent sources of carotenoids. Although komatsuna (Brassica rapa var. perviridis) is a green leafy vegetable that is commonly consumed in Japan, little is known about its lutein content. Therefore, we investigated the lutein content of six komatsuna cultivars and the influence of hot air drying. The lutein content of the six komatsuna cultivars ranged from 3.7 to 5.4 mg/100 g FW, and it correlated with the SPAD values, which indicate chlorophyll content. Blanching of the komatsuna before hot air drying prevented the lutein content from decreasing. The lutein content of komatsuna powder that was produced in our laboratory ranged from 0.66 to 0.92 mg/g DW. When the komatsuna powder was stored at -30 ℃, the lutein content remained stable for 6 months. [ABSTRACT FROM AUTHOR]

Indigenous leafy vegetables such as the spider plant (Cleome gynandra L.) can play an important role in alleviating malnutrition and providing a source of income to smallholder growers. In South Africa, the spider plant is often harvested from the wild. There is lack of information regarding the perceptions of end-users about this indigenous leafy vegetable in many regions of South Africa. Therefore, this study was designed to evaluate the perceptions of local communities about the spider plant in Limpopo Province (South Africa). This information on the perception of spider plant by end-users will serve as a pre-requisite to establish a breeding programme, strategies to conserve the vegetable and to establish commercial production of this vegetable. Questionnaires were administered to households in each of the five districts in Limpopo Province targeting a wide range of end-users. Their consumption patterns, availability and level of knowledge of spider plant was assessed. Chi-square tests were used to assess the differences in perceptions and consumption patterns. The results showed that some of the communities relied on harvesting the vegetable spider plant from the homestead for consumption. There was no commercial seed production of this vegetable in these communities. The highest consumption (> 50%) of the vegetable was observed in Waterberg and Sekhukhune districts. The results also showed that the age of consumers influenced dietary preferences with the old people preferring this leafy vegetable more than the young people. The bitter taste of the vegetable appeared to deter some potential end-users. These results showed that there is potential to cultivate the spider plant as a crop and to improve the taste of the vegetable. In addition, good quality seed could improve the production of the vegetable. [ABSTRACT FROM AUTHOR]

Flowering Chinese cabbages are widely planted in the south of China and often exposed to acid rain. However, the effect of acid rain on the growth of flowering Chinese cabbage is unclear. In this study, we investigated the influence of simulated acid rain (SAR) on plant height, soil-plant analysis development (SPAD) value (an index for chlorophyll content), proline, malondialdehyde (MDA), antioxidant enzyme activities, nitrogen (N), phosphorus (P), or potassium (K) uptake and variation of soil nutrients. Our results showed that SAR at pH 5.5 did not damage plant development because growth characteristics, photosynthesis, and superoxide dismutase and peroxidase activities did not change obviously at this pH compared to those at pH 7.0. However, 2- to 7-time of SAR exposure at pH 4.5 and pH 3.5 led to the increases of antioxidant enzyme activities, MDA and proline contents, and the decreases of leaf SPAD value and root activity. Nutrient analysis indicated that spraying 4 to 7 times of SAR at pH 3.5 reduced the uptake of N, P and K of flowering Chinese cabbage significantly. In addition, treatment with SAR at pH 3.5 decreased the pH value of the surface soil and the contents of alkaline-hydrolytic N and readily available K but increased that of readily available P in the surface soil by 8.5% to 14.9%. Taken together, our results indicated that SAR at pH 3.5 influenced the antioxidant enzyme system and the contents of soil nutrients, caused metabolic disorders and ultimately restricted the development and growth of flowering Chinese cabbages. [ABSTRACT FROM AUTHOR]

Cleome gynandra, a leafy vegetable consumed among all age-groups, is often limited by its inherent bitter taste due to the chemical compound, proanthocyanidins (PAs), commonly known as condensed tannins (CT), located in the vegetative parts of the plant. Therefore, a study was conducted during 2017 at Green Biotechnologies Research Centre of Excellence (GBRCE), University of Limpopo, South Africa to determine the optimum harvesting time and PAs concentration level in C. gynandra leafy vegetable, unsuitable for consumption purposes. Pre-chilled C. gynandra seeds were directly sown in 20-cm-diameter plastic pots filled with 10,000 cm3 mixture of steam pasteurised loam soil and Hygromix- T growing media at 3:1 v/v. Pots were placed on greenhouse benches at an inter- and intra-row spacing of 20 cm and irrigated to field capacity with 250 ml of chlorine-free tapwater. Irrigation was then scheduled for every other day. Harvesting time viz., 4, 6, 8, 10 and 12 weeks represented treatments, while week 4 served as control. The study was arranged in a completely randomised design, in triplicates. Harvested material from each treatment was oven dried at 60°C for 24 hrs prior to PAs chemical analysis (Butanol-HCl method). The generated relationships were modelled through the regression curve estimates from the quadratic equation (Y = b2x2 + b1x + c), to obtain the optimum harvesting time and PAs concentration. Harvesting time had highly significant (P≤0.01) effect on PAs concentration of C. gynandra tender shoots and leaves. Treatments contributed 99% of the total treatment variation in PAs concentration of the harvested material. Optimum harvesting time was achieved at 9 weeks after planting, with optimum PAs concentration of 89%. Therefore, for consumption purposes, harvesting of C. gynandra leafy vegetable should be done between week 4 and week 9 after planting, when PAs concentration is not at peak. [ABSTRACT FROM AUTHOR]

Information is needed for comparative assessment and agronomic practices for phytoavoidation in multi-pollutant field. A field study was conducted to explore 97 Brassica pekinensis L. genotypes with permissible limit of contaminants growing in a severely Cd, moderately nitrate and slightly Pb multi-polluted field. Thirteen genotypes, i.e. KGZY, CXQW, CAIB, JINL, JQIN, JFEN, WMQF, XLSH, TAIK, BJXS, JUKA, XYJQ and GQBW, were identified with permissible limit for nitrate, Cd and Pb based on their resistance to heavy metal and nitrate accumulation in leaves when grown in co-contaminated soils. Furthermore, the correlation between essential and toxic elements concentrations in plant of B. pekinensis were inconsistent. Generally speaking, application of increasing Ca, K and S fertilizers in appropriate forms and dosages tended to increase the yield and quality of B. pekinensis cultivated in multi-pollutant field. [ABSTRACT FROM AUTHOR]

Contamination of edible produce leaves with human bacterial pathogens has been associated with serious disease outbreaks and has become a major public health concern affecting all aspects of the market, from farmers to consumers. While pathogen populations residing on the surface of ready-to-eat produce can be potentially removed through thorough washing, there is no disinfection technology available that effectively eliminates internal bacterial populations. By screening 303 multi-gene deletion (MGD) mutants of Salmonella enterica serovar Typhimurium (STm) 14028s, we were able to identify ten genomic regions that play a role in opening the stomatal pore of lettuce leaves. The major metabolic functions of the deleted regions are associated with sensing the environment, bacterium movement, transport through the bacterial membrane, and biosynthesis of surface appendages. Interestingly, at 21 days post inoculation, seven of these mutants showed increased population titers inside the leaf, two mutants showed similar titers as the wild type bacterium, whereas one mutant with a large deletion that includes the Salmonella pathogenicity island 2 (SPI-2) showed significantly impaired persistence in the leaf apoplast. These findings suggest that not all the genomic regions required for initiation of leaf colonization (i.e., epiphytic behavior and tissue penetration) are essential for continuing bacterial survival as an endophyte. We also observed that mutants lacking either SPI-1 (Mut3) or SPI-2 (Mut9) induce callose deposition levels comparable to those of the wild type STm 14028s; therefore, these islands do not seem to affect this lettuce defense mechanism. However, the growth of Mut9, but not Mut3, was significantly impaired in the leaf apoplastic wash fluid (AWF) suggesting that the STm persistence in the apoplast may be linked to nutrient acquisition capabilities or overall bacterial fitness in this niche, which are dependent on the gene(s) deleted in the Mut9 strain. The genetic basis of STm colonization of leaves investigated in this study provides a foundation from which to develop mitigation tactics to enhance food safety. [ABSTRACT FROM AUTHOR]

Amaranthaceae family has a valuable number of over 600 species, but in Romania is not so much cultivated. The plant has multiple uses: decorative, food, medicinal and energetic, but also is a weed in the wild flora. VRDS Buzau, through Breeding and Biodiversity Laboratory, has shown more interest for this plant, realizing until now a valuable germplasm collection. In this study were selected 11 genotypes of Amaranthus from the germplasm collection. The research aimed was to study the adaptability to the pedoclimatic conditions in south of Romania and analysing the agronomic traits. The agro-morphological characters were statistically analysed and significant differences were found in the diameter of the bush, the length of the panicle, as well as the number of main branches. The genotypes had different types of the inflorescences, thus 9.1% were semi-drooping, completely drooping 36.5% and straight inflorescences 54.4%.The colour of the inflorescence also had variations from light green to dark pink. In conclusion, V.R.D.S. has a large variety of genotypes in this species and the research will continue by enriching the germplasm collection in order to obtain new cultivars. [ABSTRACT FROM AUTHOR]

The use of organic or reduced form of nitrogen (N) can have various beneficial effects in terms of plant nutrient uptake, metabolism, and environmental issues. In this study, the influence of soil application of reduced N-forms (ammonium, glycine, and glutamine) compared to nitrate and a no fertilizer treatment was evaluated on growth characteristics of sweet basil (Occimum basilicum L.) under a moderate lime soil conditions. The basil growth traits including root and shoot biomass were increased under application of reduced N-forms mainly glycine and glutamine compared to no fertilizer treatment. Application of reduced forms of nitrogen (ammonium, glycine, and glutamine) increased the leaf concentrations of potassium (K), magnesium (Mg), calcium (Ca), iron (Fe) and zinc (Zn), whereas the leaf N concentration was increased by ammonium and nitrate fertilization compared to unfertilized control plants. The results indicate that soil application of reduced N-forms particularly glycine and glutamine is superior to nitrate application. [ABSTRACT FROM AUTHOR]

There is always a need for novel, high quality, functional and inexpensive foods among consumers in the global markets. Leafy vegetables can fulfill such needs. Leafy vegetables are now used worldwide as food for their nutritional and medicinal values. In the present work an ethnobotanical survey was carried out on the utilization of edible plants by local communities of the Terai-Dooars Region of West Bengal. The information has been documented by interviewing traditional farmers, herbalists, various older men and women following different ethnobotanical methods. A total of 103 plant species under 44 families with their short botanical description, use, range of demands and cultivation status have been documented. [ABSTRACT FROM AUTHOR]