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The applications of ozonized oils in human medicine, veterinary, and dentistry have been experiencing a significant increase in the last years. An important chemical property of ozonized oils is their oxidizing capacity, which is directly related to the therapeutical effects. This work aimed to study the light emission caused by the oxidation of luminol by ozonized sesame oil. Sesame oil was ozonized at different time intervals and characterized by peroxide index, Fourier-transform infrared spectroscopy, and thermal analysis. The light emission efficiency was correlated with the pH of the medium, the concentration of horseradish peroxidase (HRP), used as a catalyst, and solvents. A linear correlation was obtained between the concentration of ozonized oil and the integrated light emission. In a second approach, taurine chloramine (Tau-NHCl) was used instead of HRP. The light emission was dependent on both ozonized oil and Tau-NHCl, showing the involvement of intermediate oxidative species in the reaction. Considering the growing therapeutic applications of ozonized oils and Tau-NHCl, these findings instigate further biological investigations to characterize additive or synergic microbicidal properties. [ABSTRACT FROM AUTHOR]

Ozone therapy delivered by local injections, together with topically applied ozonated vegetable oil, to a woman with chronic, extremely painful, and swollen anal fissure provided rapid relief of symptoms. All symptoms were abated by 14 sessions. Full healing occurred, which to date has endured over 2 years. Ozone by injection may be a superior form of delivery of this healing gas than rectal insufflation for anal fissure. The treatment is inexpensive, relatively non-invasive, and can be performed without pain under local anesthesia. [ABSTRACT FROM AUTHOR]

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The aim of this study is the determination of antimicrobial activity at different concentrations of ozonized olive oil of Olea europea L. against eight bacteria (Staphylococcus aureus ATCC 6538, Bacillus subtilis ATCC 9372, Listeria monocytogenes ATCC 15313, Pseudomonas aeruginosa ATCC 27853, Proteus mirabilis ATCC 35659, Escherichia coli ATCC 25922, Klebsiella pneumonia ATCC 4352, Salmonella typhimurium ATCC 13311), two yeasts Candida albicans ATCC 25923 and Aureobasidium pullulans and five fungi Aspergillus niger ATCC 16404 Penicillium digitatum, Penicillium expansum, Aspergillus itaconicus, Fusarium solani. The antimicrobial activity of ozonized oil is measured by the agar diffusion method. The oil inhibits the growth of most strains, Proteus mirabilis ATCC 35659 as the most sensitive bacterium with the inhibitory zone of 18.33 mm±0.47. The results of the minimal inhibitory concentration (MIC) test showed that the MIC varies from 0.198 mg/mL to 63.5 mg/mL. The ozonized oil inhibits the growth of all tested fungal strains, the most sensitive fungi is Fusarium solani with the inhibitory zone of 36.66 mm±0.40 with minimal fungistatic concentration (MFCs) and minimal fungicidal concentration (MFCc) obtained between 0.38 mg/mL and 12.2 mg/mL. The CMF of the oil ozonized on C. albicans obtained is 0.38 mg/mL, 13 times less compared to that found in the study, this result indicates that antifungal activity on C. albicans of ozonized oil is very significant. We demonstrate that the ozonation of olive oil leads to the formation of the various toxic products (hyperoxides, ozonides, adehhydes, peroxides, diperoxides and polyperoxides) responsible for the antimicrobial effect, this effect may be due to toxicity rather than metabolic interruption. Ozone and its derivatives are proposed especially ozonide as powerful antimicrobial agents especially in this pandemic of COVID-19. [ABSTRACT FROM AUTHOR]

The use of ozonized vegetable oils as drugs or cosmetics requires having data on their stability over the time. In this study, ozonized sunflower oil was stored under different temperatures for up to 1 year. Peroxide index, acidity value, gas chromatography profile, infrared profile and antibacterial activity (againstStreptococcus uberis) of this ozonized oil were followed. The results highlight the fact that the better way to preserve the initial composition of ozonized oil is to keep it at low temperature. However, the antibacterial activity is not diminished by the changes occurring to the composition. [ABSTRACT FROM PUBLISHER]

Ozonized oils represent an interesting pharmaceutical approach to the management of a variety of dermatological pathologies. Ozone reacts with carbon-carbon double bonds of unsaturated fatty acids according to the mechanism described by Criegee, forming ozonides or 1,2,4 trioxolane rings and peroxides as the most important products, responsible for the antimicrobial activity and stimulating tissue repair and regeneration properties. The ozonized vegetable oils can be liquids or semisolids at room temperature and have stability periods that may be adequate for commercial distribuition. Ozonized sunflower oil (Oleozon®), a drug registered nationally and developed in the Ozone Research Center in Cuba has been tested and it was found to have valuable antimicrobial activity against bacteria, fungi and virus. FT-IR and NMR technics are used to confirm the structural changes undergone by oil during the ozonation. For determining the quality of ozonized oils, analytical methods such as peroxide, acidity and iodine values are usually carried out. Products are available for an alternative use of available resources, natural and renewable sources, simple technology, low cost and with extensive biological activity with reduced collateral effects. [ABSTRACT FROM AUTHOR]

Simple Summary: Cancer relapses after chemo-radiotherapies arise from cancer stem cells able to escape cell killing because of their high antioxidants level. The aim of this study was to test the efficacy of ozonized oils to decrease the rate of cancer relapses. In vitro, oils at high ozonide content penetrate inside cancer cells releasing oxygen and reactive oxygen species damaging the thin outer membrane of inactive mitochondria. This event triggers intracellular calcium release and activates apoptosis. In vivo, ozonized oil has been administered by the oral route effectively decreasing blood antioxidants in cancer patients. This approach results in significant increase of survival rate and decrease of relapses in 115 cancer patients (brain, lung, pancreas, colon, skin) undergoing standard radio-chemotherapy regimens during a 4-years follow up. Obtained results indicate that the administration of ozonized oil represents an integrated approach to decrease the risk of radio-chemoresistance and cancer relapses in cancer patients. Background: Cancer tissue is characterized by low oxygen availability triggering neo angiogenesis and metastatisation. Accordingly, oxidation is a possible strategy for counteracting cancer progression and relapses. Previous studies used ozone gas, administered by invasive methods, both in experimental animals and clinical studies, transiently decreasing cancer growth. This study evaluated the effect of ozonized oils (administered either topically or orally) on cancer, exploring triggered molecular mechanisms. Methods: In vitro, in lung and glioblastoma cancer cells, ozonized oils having a high ozonide content suppressed cancer cell viability by triggering mitochondrial damage, intracellular calcium release, and apoptosis. In vivo, a total of 115 cancer patients (age 58 ± 14 years; 44 males, 71 females) were treated with ozonized oil as complementary therapy in addition to standard chemo/radio therapeutic regimens for up to 4 years. Results: Cancer diagnoses were brain glioblastoma, pancreas adenocarcinoma, skin epithelioma, lung cancer (small and non-small cell lung cancer), colon adenocarcinoma, breast cancer, prostate adenocarcinoma. Survival rate was significantly improved in cancer patients receiving HOO as integrative therapy as compared with those receiving standard treatment only. Conclusions: These results indicate that ozonized oils at high ozonide may represent an innovation in complementary cancer therapy worthy of further clinical studies. [ABSTRACT FROM AUTHOR]