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Encapsulation plays a vital role in the food industry, known for its multifunction constituents’ preservation, covering undesirable food components (taste, color, flavor), nutritional and functional components incorporation, and under controlled conditions (time and rate) release of encapsulation ingredients. Milk proteins are highly demanding encapsulating material and are investigated at large scale to design encapsulating devices. Polyphenols, flavorings, fatty acids, minerals, and hydrophobic vitamins are within encapsulating bioactives. Milk protein is widely used in the microencapsulation (ME) of probiotics and in comparison to other biomaterials offers more benefits. Milk protein includes whey protein and casein and several techniques are developed to make use of them in the ME of multiple probiotic strains (Bifidobacterium, Lactobacillus). This review will cover all the possible aspects of dairy proteins and their properties that enabled them to be used as encapsulating material. This review will also discuss the range of hydrophobic and hydrophilic components delivered from milk protein and their utilization in the formation of encapsulating agents. Overall, this study aimed to explore the emerging role of encapsulation in the food industry, characterize milk proteins as effective encapsulation agents, investigate their synergism with nondairy encapsulating materials, and evaluate various encapsulation techniques.

The purpose of this research is to evaluate the risk of metals (lead, chromium, mercury, arsenic and nickel) on the health of children and adults through the consumption of Nile Tilapia cultured in the Biofloc breeding system. The number of 30 pieces of Tilapia grown in the Biofloc system was caught and analyzed by an atomic absorption device equipped with a graphite furnace system. The average concentration of metals, the daily intake (DI) of metals through the frequency of consumption of Tilapia for different groups (adults and children with an average age of 10 years), the risk index(HQ), the number of allowed servings of Tilapia per month(CRmm) was calculated for people in different groups. The highest and lowest daily absorption rates in the adult group are related to arsenic (2.08×10-3 µg/kg/d) and mercury (2.49×10-6 µg/kg/d) in the children group, respectively in the case of lead (4.93×10-5 µg/kg/d) and mercury (3.28×10-9 µg/kg/d). The hazard index (HQ) for both study groups was lower than one in all elements. The HI rate was 0.83 for adults and 0.02 for children, which was lower than one. For adults, the HI index was close to 1, which according to the HQ values of each element, the greatest increase in the HI value can be due to the higher HQ value for arsenic.

In this study, tempeh was developed using a probiotic culture of Lactobacillus rhamnosus adjunct culture. Purposely, soybean seeds were soaked overnight at room temperature and then boiled at 100°C for 10 to 15 min. The hot beans were then spread out in a thin layer and allowed to cool to room temperature. The Lactobacillus rhamnosus culture was added to the soybeans, which were then tightly packed in perforated plastic bags and incubated at 30°C for 24 h to produce fresh tempeh. The tempeh was then analyzed for its physical, chemical, microbial, and sensory characteristics, and the data obtained was statistically analyzed. The results of the physical and chemical analysis showed that the probiotic tempeh had a slight increase in pH, ash, and protein content, while there was a significant decrease in acidity, moisture, and fat content during storage. The microbial analysis revealed that both the total plate count and probiotic count increased over time, with treatment T4, which contained a high amount of culture, exhibiting the maximum plate count and probiotic count. The sensory evaluation showed that both the treatment and storage duration significantly impacted the aroma, color, taste, and overall acceptability of the tempeh, with all sensory attributes decreasing over time. The sensory panelists rated treatment T4 the highest for overall acceptance. In conclusion, the study suggests that probiotic soybean tempeh could be a beneficial carrier for producing a product with good physicochemical properties, acceptability, high probiotic viability, and storage durability.

This comprehensive review paper delves into the multifaceted aspects of nuclear safeguards, emphasizing their indispensable role in upholding global security and preventing the illicit use of nuclear materials. Offering a fresh perspective on the nuclear safeguard system, the paper conducts a thorough examination of nuclear material accounting, underscoring the pivotal role of the International Atomic Energy Agency (IAEA) in implementing and verifying these safeguards. It provides valuable insights into the safeguarding technologies, including the utilization of sophisticated Non-Destructive Assay (NDA) equipment such as neutron counting and Gamma-ray spectroscopy, which are essential for accurately characterizing nuclear materials, elucidating the crucial role of these activities in ensuring the integrity and effectiveness of nuclear safeguards. Furthermore, the study sheds light on the complexities inherent in the nuclear fuel cycle, elucidating various stages ranging from uranium mining to waste disposal. Additionally, it discusses different fuel cycle options and their implications for non-proliferation efforts. This discussion sheds light on how safeguards are integrated into each stage of the fuel cycle to prevent the proliferation of nuclear materials. The paper delves into the Additional Protocol, emphasizing its significance in enhancing the effectiveness of nuclear safeguards by providing the IAEA with expanded inspection access and information regarding nuclear activities. This comprehensive analysis not only enhances understanding of the current safeguarding landscape but also underscores the significance of ongoing research and development in this vital realm of nuclear safety and security.

After the Fukushima-Daiichi event, there has been notable progress in developing accident-tolerant fuel (ATF) cladding designs to improve light-water reactor (LWR) safety features. In this regard, the Zirconium alloy (Zircaloy) has significant advantages in enhancing the neutron economy, and its unique beneficial characteristics establish it as a reliable and promising material for ATF claddings in the present era. Depositing protective coatings of different interlayer diffusion metals over Zircaloy-based nuclear fuel claddings is becoming increasingly popular as a near-term solution. This strategy can enhance the ability of cladding to withstand accident scenarios, reduce the oxidation rate at high temperatures, and provide additional benefits during both usual and accidental conditions. Current research efforts have strategically prioritized the development of ATF cladding concepts to improve nuclear fuel safety in normal, transient, and potential accidental situations. Herein, we present a comprehensive review of the current research and development events related to the design, manufacturing techniques, and various performance characteristics of chromium (Cr) coated Zircaloy-cladding ATF materials. These materials can improve both the reactor's economics and protection level. Our discussion focuses on these methods for enhancing safety performance in LWRs. In addition, the report provides a prospective viewpoint on future research accomplishments related to these materials.

Objective Diffuse large B-cell lymphoma (DLBCL) is an aggressive type of lymphoma. Circulating cell-free DNA (cfDNA) is the promising liquid biopsy in malignancy.Method Assessment of cfDNA concentration and DNA integrity index (DII) in 30 newly diagnosed DLBCL patients and correlating them with the international prognostic index.Results Circulating cfDNA concentration was significantly higher in newly diagnosed DLBCL patients than controls, while DII was significantly lower. There was a significant correlation between cfDNA concentration and adverse prognostic factors.Conclusion cfDNA concentration is potentially strong diagnostic and prognostic marker of DLBCL, however DII is only useful as a diagnostic biomarker.

Objective To determine the effects of Dextrose 5% for maintenance and creep fluids on cumulative fluid balance (CFB) for septic shock concerning adult people.Design An open-label randomized clinical trial with a single center and parallel groups.Setting ICUs of a tertiary care academic medical center.Patients Adult individuals who have developed septic shock as described by the Sepsis 3 criteria.Interventions Dextrose 5% (n = 50) versus Saline 0.9% (n = 50) for maintenance and creep fluid for 72 hours.Main variables of interest The primary outcome measured was the CFB at 72 hours. Secondary outcomes assessed included mortality within 28 days, the number of days patients were free from (coma and delirium, ICU, mechanical ventilation, vasopressors), the occurrence of acute kidney injury, and disturbances in electrolyte levels.Results The CFB was significantly lower in Dextrose group vs Saline group (4.46 versus 6.75 L, median difference; −2.06 L; 95% CI, −2.63 to −1.35; p = P

Background AML is a complex and heterogeneous disease. The KRAS gene is one of the important genes in the pathogenesis of acute myeloid leukemia (AML). Mutant RAS can promote oncogenesis via different mechanisms including oncogenic transcription, cell cycle progression, cellular survival, growth, metabolism, and cell migration. Therefore, it is important to identify the genomic landscape of AML. The aim of the study is to identify KRAS variants in AML and their association with clinic pathological criteria and possible effects on prognosis using NGS.Method Hotspot mutations in the KRAS gene were studied using Ion S5 next-generation sequencing system. Bone marrow samples of newly diagnosed AML patients were collected to identify hotspot mutations in the KRAS gene. DNA amplicons were subjected to sequencing and were analyzed using ion torrent software. Patients were classified according to the FAB classification system. Patients are also classified according to the cytogenetic groups and the ELN risk stratification system.Results KRAS mutations were detected in exon 2, 3, whereas no mutations in KRAS exon 1. Interestingly, Novel mutations were detected in KRAS in AML Egyptian patients. Also, there was no statistically significant association of RAS mutations with different clinical and prognostic parameters. However, KRAS mutant patients tended to have increased PB WBC counts, percentage of PB, and bone marrow blasts.Conclusion NGS is considered a useful tool to identify KRAS variants that could be useful for risk stratification and tailored therapy in AML patients

This review article studies the complex field of noncoding RNAs (ncRNAs) in cancer biology, focusing on their potential use as biomarkers and therapeutic targets. NcRNAs include circular RNAs (circRNAs), long noncoding RNAs (lncRNAs), and microRNAs (miRNAs). We discuss how ncRNAs affect gene expression in cancerous cells, the spread of cancer, and metastasis. The article illustrates the complex pathways through which ncRNAs can affect oncogenesis, tumor suppression, and therapeutic resistance. It also assesses the potential clinical uses of non-coding RNAs (ncRNAs), including their utility in cancer diagnosis, and prognosis. Besides, their potential for personalized cancer therapy is documented. Finally, it is concluded that understanding the role of ncRNA is of clinical importance regarding carcinogenesis, cancer diagnosis, and personalized therapy.

Aim of the Work Aim of the work is to evaluate the effect of intracavernosal injection of platelet-rich plasma (PRP) for the treatment of arteriogenic erectile dysfunction (ED).Methods This study was conducted up on 25 men complaining of mild-to-moderate ED, who were in a stable relationship for more than 1 year.Results After 6 months of intracavernosal PRP injection, there was a statistically significant improvement of the International Index of Erectile Function-Erectile Function domain score, which then declines after 1 year of the treatment. No adverse effects were observed during the study.Conclusions PRP intracavernosal injections may be a promising option for the management of arteriogenic ED. But further studies are needed to confirm these findings.

Background: The global promotion of herbal products is not at par due to a lack of quality control studies. Marham-i-Raal, a pharmacopoeial ointment, treats various types of ulcers and wounds. The present study evaluates the quality control, antimicrobial, and antioxidant properties of Marham-i-Raal. Methods: The quality control parameters, including loss of weight on drying, pH, total ash value, acid-insoluble ash value, heavy metal analysis, microbial load, and high-performance thin-layer chromatography of Marham-i-Raal, were carried out. Moreover, the antimicrobial and antioxidant activities of Marham-i-Raal were also investigated. Results: The quality control findings of Marham-i-Raal, including weight loss on drying, pH, total ash value, acid-insoluble ash value, heavy metal analysis, and microbial load, were found within the normal limits. The antimicrobial study of Marham-i-Raal has shown promising effects against gram-positive bacteria and Candida albicans. The antioxidant effects of the test drug were also found to be significant at higher concentrations against 2,2-diphenyl-1-picrylhydrazyl and 2,2’-azino-bis-(3-ethylbenzothiazoline-6-sulfonic) acid methods. Conclusion: The standards developed for the quality control of Marham-i-Raal may be referred to in the future for further studies. This important Unani ointment may be clinically used to treat non-healing ulcers and wounds.

Background Obesity causes changes in the production of adipose tissue secreted factors, which are responsible for metabolism modulation. One of these factors is Apelin, a small peptide that interacts with a specific cell-surface receptor to modulate various cells signaling processes. Apelin is regarded as a new player because of its powerful role in energy metabolism and improvement of insulin sensitivity.Aim of the Work This study investigates the plasma apelin 36 role and to what extent it can impact the insulin resistance in obese Egyptians diagnosed with type 2 diabetes (T2DM).Subjects and Methods The study involved 90 participants, with 30 assigned to each of the three study involved categories: obese individuals with diabetes, obese individuals without diabetes, and healthy controls (n = 30 for each group). Measurements of skin fold thickness, body mass index (BMI), waist/hip ratio, and body fat % were obtained along with a comprehensive clinical examination and history. The following were investigated: plasma insulin, Hb A1c, lipid profile, plasma apelin (ELISA), plasma leptin (ELISA), and plasma adiponectin (ELISA).Results The median value of serum leptin in obese subjects (groups I and II) was higher significantly than healthy control group (group III) (p =

The notion of teacher commitment has grabbed much attention in the mainstream, and L2 teacher education as teaching has become more complex by recent sociocultural changes in educational practices. Consequently, several instruments have been developed to operationalize the construct. The instruments developed so far have addressed the construct generically. To be sure, this line of inquiry is still untouched in the ELT profession. The present exploratory mixed methods study attempted to develop a questionnaire to measure English language teachers’ commitment. In doing so, an initial 61-item questionnaire was developed conducting a comprehensive literature review and using interviews with domain experts and English language teachers. Then the trial instrument was administered to a sample of 352 teachers for Exploratory Factor Analysis (EFA), reducing the instrument to 32 items. The subsequent Confirmatory Factor Analysis (CFA) obtained from another sample of 577 individuals substantiated a seven-factor model as a robust and valid tool for measuring English language teacher commitment. The seven-factor model of teacher commitment included cognitive language teaching ability, language classroom environment, job satisfaction, opportunities for professional development, language teaching planning and support, language teaching self-image and beliefs, and language teaching exhaustion. ELTCQ can be used for measuring English teachers’ commitment. Researchers, administrators, and teacher trainers can use the questionnaire to improve the quality of in-service courses and examine teacher qualities for future professional predictions.

Neurological and cardiovascular illnesses, cancers, metabolic disorders, and microbial infections result in deaths of millions of people globally. Recent researches have intensely been directed at evaluating the therapeutic potential of herbal plants as alternative medicines. One such species is chamomile (Matricaria chamomilla), from the Asteraceae family. It elicits protective properties against inflammation and oxidative stress, bacterial and fungal infections, cancers, ulcers, neuropathologies, etc. The present review focuses on various medical/ethnomedicinal uses of chamomile. Moreover, we emphasize on the therapeutic actions of constituents from chamomile, along with their underlying mechanism(s). Literature was systematically searched on electronic databases using keywords; “Matricaria chamomilla/chamomile” in combination with “medicinal,” and “therapeutic.” Our analyses of the retrieved articles indicate tremendous therapeutic potential of chamomile, and establish it as an alternative multimodal herbal medicine. The individual active constituents (e.g., geraniol, caffeic acid, chlorogenic acid, umbelliferone, rutin) were also found to elicit significant beneficial properties in various disorders. Collectively, chamomile and its bioactive seem to hold great promises as miraculous herbal drugs with multiple medical uses. In conclusion, based upon its beneficial antibacterial, antioxidant and anti-inflammatory actions, Matricaria chamomilla is strongly implicated in protection against skin photo-damage, aging, neuropathologies and cancers.

Transportation and logistics play a crucial role in the overall supply chain of a country by ensuring the timely delivery of goods for production and consumption. The current study investigates the relationship between economic growth and logistics performance across countries. The study’s significant contribution is using logistics performance data from a panel of 107 countries covering the period from 2007 to 2019 and applying panel data-based time-series econometric models to investigate the link between economic growth and logistics performance. The study confirms a long-term relationship between economic growth and logistics performance and shows that short-term disequilibrium is adjusted back to steady-state conditions. The findings suggest that policymakers should focus on developing all aspects of logistics performance rather than physical infrastructure.

Abstract Purpose Antihyperglycemic drug artemisinin (Art) has recently gained attention as a potential anticancer treatment. In this study, the poly(lactic-co-glycolic acid) (PLGA) polymer was used to create Art-containing nanofibers (NFs) using the electrospinning technique. Methods The morphological characteristics, rate of degradation, and drug release profile of the NFs were described. In addition, we examined both the cytotoxic effects and internalization of reactive oxygen species (ROS), as well as the expression levels of apoptotic genes following the treatment of SK-BR-3 breast cancer cells with Art and Art-loaded PLGA nanofibers. Results The bead-free, smooth surface, and randomly aligned electrospun NFs released the medication quickly at first and then steadily for more than 2 weeks. They also showed a rather steady rate of deterioration over the course of 24 days. After 48h, SK-BR-3 cells exposed to ART-loaded NFs shown a substantial cytotoxicity compared to free Art. Additionally, Art-loaded NFs effectively increased intracellular ROS levels, inducing death in cancer cells. Gene expression studies further demonstrated the ability of the produced Art-loaded NFs to significantly modify Bax and Bcl-2 levels as well as activate caspases-3 and P53 compared to free Art. Conclusion Overall, the results demonstrated the Art-loaded PLGA nanofibers anticancer effectiveness, indicating that it may be employed as an implantable drug delivery system to decrease breast cancer recurrence following surgical resection.

Abstract Background Choline was frequently reported to have some beneficial effects on memory function. However, the association of dietary choline with different types of amnesia is not well understood. The objective of this study was to examine the association between dietary intake of choline and transient global amnesia (TGA). Methods This case–control study was carried out on 258 patients with TGA and 520 participants without amnesia. Data on dietary choline intake was collected using a validated food frequency questionnaire (FFQ). All participants were examined for amnesia by a neurologist according to the Kaplan and Hodges criteria. Results There was an inverse association between TGA and dietary choline intake after adjustment for age and gender (OR: 0.98, CI 95% 0.96–0.98, P = 0.03). The association remained significant after additional adjusting for physical activity, body mass index (BMI), occupation, marital status, smoking, and drinking alcohol (OR: 0.98, CI 95% 0.96–0.99, P = 0.04) and after further adjustment for calorie and food groups intake (OR: 0.98, CI 95% 0.96–0.99, P = 0.03). Conclusion The results of this study indicated that choline may have beneficial effects against TGA. Further longitudinal studies are warranted. Graphical Abstract

Abstract This study assessed sustainable solutions for organic waste management, focusing on biochar derived from kitchen waste. The characteristics and phytotoxicity effects of biochar produced from four different types of kitchen waste were investigated in view of potential agricultural applications. Analysis of the chemical and physical properties of the different biochar samples by X-ray fluorescence and Fourier transform infrared spectroscopy revealed a nutrient-rich composition with carbon, calcium, and potassium contents that ranged from 35 to 48%, from 1.6 to 24%, and from 1.5 to 28.5%, respectively. In phytotoxicity tests, the highest germination rate (45%) was observed with Coffee residue biochar obtained at 300 °C (application rate of 1%) and the longest shoot length (25 cm) with orange peel biochar obtained at 300 °C (application rate of 1%). Germination rate and shoot length were not significantly different between biochar-exposed soils and control soils (without biochar), indicating no toxic effect due to biochar addition. Washing biochar improved germination rates significantly (control: 98%; potato peel biochar: 92%; banana peel biochar: 83%). The longest shoot length (8.3 cm) was obtained with the washed potato peel biochar (pyrolysis temperature = 400 °C) extract. These findings suggest that biochar can be safely used as a soil amendment without harming the environment or hindering plant growth. The Tukey honestly significant difference (HSD) test results further emphasized the influence of different factors, such as pyrolysis temperature and feedstock type, in biochar applications.

Abstract The increase in wind power‐based microgrids emphasizes the importance of addressing stability challenges during low‐voltage ride‐through (LVRT) events in weak AC grid‐connected doubly fed induction generator systems. Compliance with grid standards, notably LVRT capabilities, is critical as wind power plants integrate increasingly into power systems, raising concerns about generation loss and post‐fault oscillations in microgrids. Previously, researchers have utilized techniques like fuzzy logic, ant colony, and genetic algorithms for static synchronous compensator (STATCOM) tuning to enhance microgrid stability during fault scenarios. This study uses the grasshopper optimization algorithm (GOA), particle swarm optimization (PSO), and a novel hybrid GOA‐PSO. On the main grid, the power system is subject to both symmetrical and asymmetrical faults. The proposed novel technique aims to improve LVRT, minimize generation loss during faults, and reduce after‐fault oscillations by optimizing reactive power flow between the point of common coupling and the microgrid while adhering to the LVRT grid code. MATLAB/Simulink is utilized to evaluate the LVRT performance of a 16 MW DFIG‐based microgrid operating in grid‐connected mode. The performance of the GOA‐PSO‐tuned STATCOM is evaluated by comparing it with conventional, PSO, and GOA‐tuned STATCOM in three fault scenarios. The comparison shows that GOA‐PSO‐tuned STATCOM improves grid stability and reliability.

Abstract Background Genomic epidemiology has helped reconstruct the global and regional movement of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, there is still a lack of understanding of SARS-CoV-2 spread in some of the world’s least developed countries (LDCs). Methods To begin to address this disparity, we studied the transmission dynamics of the virus in Bangladesh during the country’s first COVID-19 wave by analysing case reports and whole-genome sequences from all eight divisions of the country. Results We detected > 50 virus introductions to the country during the period, including during a period of national lockdown. Additionally, through discrete phylogeographic analyses, we identified that geographical distance and population -density and/or -size influenced virus spatial dispersal in Bangladesh. Conclusions Overall, this study expands our knowledge of SARS-CoV-2 genomic epidemiology in Bangladesh, shedding light on crucial transmission characteristics within the country, while also acknowledging resemblances and differences to patterns observed in other nations.

Abstract Background Spinal manipulative therapy (SMT) is a guideline-recommended care for musculoskeletal pain taught in various undergraduate programs. Visual feedback through force-sensing tables can improve modulation of SMT force–time characteristics and, potentially, students’ confidence, both factors important for clinical competence and patient outcomes. However, it is unclear if a link exists between students’ confidence and ability in SMT force–time modulation. We aim to investigate this relationship and whether it was moderated by experience. Methods This cross-sectional study recruited first- to third-year Canadian Memorial Chiropractic College students. Participants provided information about their confidence in performing SMT using different impulse forces of 200N, 400N, and 800N with a pre-established pre-load and a time-to-peak force

Abstract The study of genotype × environment interaction is crucial for plant breeders to introduce new cultivar(s) with improved and stable yield performance. The productivity of chickpea crops is very low in Pakistan. It requires the selection of genotypes with optimal productivity for diverse environmental conditions. Fourteen different chickpea genotypes were assessed using the linear mixed model to evaluate genotypes across four diverse chickpea growing regions, including Attock (Punjab), Bhakkar (Punjab), Karak (Khyber Pakhtunkhwa), and Larkana during the year 2017–19. The environmental effect was pronounced and contributed significantly to variation (25.8%) in grain yield. Analysing genotype × environmental interactions at multiple locations facilitates ranking superior genotypes that excel in specific or diverse environments. Notably, the genotypes, viz., Fakhr-e-Thal and Bhakkar-2011, demonstrated superior performance in terms of overall grain yield. Utilising a multitrait stability index, Bittal-2016 and Thal-2006 exhibited the most stable genotypes across various environments and were observed suitable for diverse growing regions. While, for specific environmental conditions, genotypes, i.e., KK-1, Noor-2013 and Fakhr-e-thal, exhibited higher yields and stability. This showed their adaptability to the particular environment. The present study revealed that Larkana as the mega environment was conducive to higher yield, while Attock, Bhakkar, and Karak sites were less favorable for KK-3, DG-89, and Dasht. The BLUP outperformed the AMMI in our analysis of evaluating multi-location trials. The integration of WAASBY and MTSI indices proved useful in differentiating between high yielding genotypes and those with stable performance. These tools are essential for breeders aiming to select genotypes that will perform consistently across diverse environmental conditions.

Abstract Objective The use of clear aligners (CAs) for orthodontic treatment has substantially increased in recent decades. However, limited empirical evidence exists regarding their potential effects on the temporomandibular joint (TMJ). The aim of this study was to assess the effect of maxillary molar distalization on the positional and dimensional TMJ parameters between CAs and conventional fixed orthodontic appliances (FAs) in skeletal class II malocclusion. Methods This is a retrospective cohort study examined 70 adult patients with skeletal class II malocclusion with similar baseline characteristics based on American Board of Orthodontics (ABO) discrepancy index scores. The patients were treated by maxillary distalization movement by CAs or FAs, of which there were two equal groups of 35 patients were included in each group. CBCTs on TMJs were performed before and after treatment through Anatomage Invivo Dental 6.0. The measured parameters included inclination, position, condyle and articular fossa dimensions, and the volumetric joint spaces. Results In both groups, linear measurements of the maxillary molar position revealed significant improvement after treatment (P

Background: With the exponential development of mobile data traffic and the prevalence of cloud services, optimising cloud networking has become critical for ensuring continuous connectivity. Long-Term Evolution (LTE) network integration with cloud services poses unique hurdles owing to LTE's unpredictable capacity and latency concerns. Recent advances in artificial intelligence (AI) provide promising answers to these issues, allowing for more effective cloud networking via LTE. Objective: The article intends to investigate AI-driven optimisation strategies for improving cloud network performance over LTE networks, emphasising providing continuous connectivity and lowering latency and bandwidth fluctuation. Methods: To assess and anticipate network conditions, we employ a multimethod research strategy that includes machine learning algorithms and deep learning models, allowing us to adjust cloud networking settings dynamically. Simulation tools mimic LTE network conditions and evaluate the effectiveness of AI-powered optimisation methods in various scenarios. Results: Initial results show that AI-driven optimizations significantly outperform LTE in cloud networking, with up to a 40% decrease in latency and a 25% gain in bandwidth efficiency. Deep learning algorithms for predictive analysis and real-time modifications have successfully managed network unpredictability. Conclusion: AI-driven optimizations are a realistic approach for bridging the LTE gap in cloud networking, providing considerable increases in connection, bandwidth, and latency. The article highlights AI's potential to change LTE-enabled cloud services, opening the path for more resilient and efficient cloud networking solutions.

By 2030, it is projected that over 60% of the global population will use AI-powered applications, signifying a widespread integration of AI into our daily lives. While this advancement in technology offers substantial prospects, it also triggers growing concerns over its impact on personal welfare. The increasing prevalence of AI technology used by malevolent cyberattackers poses a significant threat to individual security. The article explores the convergence of AI technology and personal safety, analyzing the risks associated with AI-driven cyber-crimes and proposing potential solutions. This report examines several artificial intelligence (AI) technologies and evaluates their vulnerabilities, highlighting statistics such as the 90% prevalence of human error in cyberattacks in 2022. Through a comprehensive analysis of existing defensive tactics, the research introduces a multidimensional structure for improving individual safety. This paradigm integrates legislative frameworks, technological solutions, and instructional activities, fostering a complete approach that addresses the problem from several angles. The proposed framework places user empowerment as the top priority via the implementation of cybersecurity education. Equipping individuals with the requisite knowledge and skills to safeguard themselves on the internet is crucial in diminishing their vulnerability to AI-fueled attacks. Practical recommendations are offered to individuals, policymakers, and technology companies to mitigate the security risks associated with artificial intelligence proactively. This article contributes to the significant discourse on ensuring the safeguarding of personal privacy and security in the age of artificial intelligence. By advocating for a holistic approach that includes legal, technical, and educational elements, it establishes the groundwork for a future that places human welfare as a top priority while still advancing technology.

Abstract In plants, the BAX inhibitor-1 (BI-1) gene plays a crucial part in controlling cell death under stress conditions. This mechanism of Programmed Cell Death (PCD) is genetically regulated and is crucial for the elimination of unwanted or damaged cells in a controlled manner, which is essential for normal development and tissue maintenance. A study on cucumber identified and characterized five BI-1 genes: CsBI1, CsBI2, CsBI3, CsBI4, and CsBI5. These genes share conserved domains, indicating common evolutionary history and function. Physicochemical analysis revealed their molecular weights and isoelectric points, while subcellular localization showed their presence in different cellular compartments. The phylogenetic analysis highlighted evolutionary relationships with related crops. Chromosomal distribution and synteny analysis suggested segmental or tandem duplications within the gene family. Protein-protein interaction analysis revealed extensive interactions with other cucumber proteins. Cis-regulatory elements in the promoter regions provided insights into potential functions and transcriptional regulation. miRNAs showed diverse regulatory mechanisms, including mRNA cleavage and translational inhibition. The CsBI3, CsBI4 and CsBI5 genes exhibit elevated expression levels during cold stress, suggesting their vital involvement in cucumber plant defense mechanisms. The application of chitosan oligosaccharides externally confirms their distinct expression patterns. The qRT-PCR confirms the upregulation of CsBI genes in ToLCNDV-infected plants, indicating their potential to mitigate biotic and abiotic stresses. The comprehensive genome-wide exploration provides opportunities for the development of cold-tolerant and virus-resistant cucumber variants by traditional breeding or gene.

Abstract Background Less is known whether the amino acid composition of dietary protein sources effects on long-term health outcomes. We aimed to evaluate the association between dietary amino acid composition and all-cause and cause-specific mortality. Methods This study used data from the Golestan Cohort Study, which was performed in the Golestan Province of Iran from January 2004 to June 2008. Mortality, which was the primary outcome, was ascertained through September 2022. The Cox proportional hazards regression models were used to determine the adjusted hazard ratios (HR) and 95% confidence intervals (CI) for mortality according to the quintiles of amino acid consumption, taking the third quintile as the reference. Results A total of 47,337 participants (27,293 [57.7%] women) with a mean (standard deviation) age of 51.9 (8.9) years were included. During a median follow-up of 15 years, 9,231 deaths were documented. Regarding essential amino acid intakes, the HRs of all-cause mortality were 1.16 (95% CI, 1.07–1.26) in the first quintile, compared with the reference group (P for non-linear trend

Abstract The rapid growth of industrialisation has led to the discharge of harmful effluents into water bodies, severely disrupting the balance of ecosystems. The detection and removal of dyes from wastewater remains a significant challenge. In this study, we report the synthesis of zinc oxide (ZnO) and its doped derivatives through a facile chemical method, followed by comprehensive characterisation and analysis to assess their potential in various applications. The structural properties of the synthesised materials were confirmed by X-ray diffraction (XRD), which verified their crystalline nature and phase purity. Morphological analysis using field emission scanning electron microscopy (FE-SEM) coupled with energy dispersive X-ray analysis (EDAX) revealed well-defined nanostructures and uniform elemental distribution. The adsorption ability of the synthesized adsorbents was investigated through electrochemical methods, cyclic voltammetry and Tafel plots, which demonstrated enhanced conductivity and charge transfer characteristics. Additionally, theoretical studies through molecular modelling and molecular dynamic simulations were employed to elucidate the interactions between Congo red molecules and the surface of the synthesized compounds. Adsorption experiments were conducted to evaluate the efficacy of ZnO and its doped variants as adsorbents. To investigate any structural or functional changes after dye adsorption FTIR and XRD were compared, provided a deeper insight into the adsorption mechanism. This multi-faceted approach highlights the potential of ZnO-based materials for effective wastewater treatment and other environmental applications.

Abstract A compact simple structure bandpass filter based on terminated three coupled line structures with high selectivity is introduced through odd and even-mode analysis. The proposed filter consists of three coupled lines loaded by open and short circuit stubs. The parallel-coupled lines structure is used to obtain high selectivity with transmission zeros near the passband. Meanwhile, simultaneously tuning on bandwidth and center frequency are introduced by coupled line. One type of bandpass filter based on terminated coupled line structure is theoretically analyzed, simulated, and measured. The stepped impedance structures at the input and output ports have been used to improve the attenuation level at the stopband region and create the multiple attenuation poles. The center frequency of 1.3 GHz, the fractional bandwidth (FBW) of about 65%, the low insertion loss of 0.25 dB, and the high return loss of 30 dB are obtained. Also, a flat group delay lower than 1.4 ns is achieved. Experimental and simulated results are presented, which are in good agreement.

Abstract Spices played crucial and variable roles in traditions, culture, history, religious ceremonials and festivals along with fetching food flavor and microbial protection globally due to presence of structurally unique and multi-natured chemotypes. Their existence in dishes portrayed key roles in improving shelf life by regulating spoilage of cuisine with different synergistic mechanism. Histo-anatomically (A) sowa exhibited distinguished cellular attributes which created remarkable differences with T. ammi. HPTLC chemometrics of both fruits revealed several peaks for active metabolomics with unique isocratic combination of menstruum. GC-MS study of hydro-distillate exhibited presence of monoterpenic cyclic and aromatic hydrocarbons, alcoholic and ketonic groups along with phenolic derivative that covers majorly 90% of total metabolites. Combined essential oils (EOs 1 + 2) of both fruits showed excellent antimicrobial activity against various clinical pathogenic strains such as K. pneumoniae at 10 µL/mL, S. aureus at 2.5 µL/mL, E. coli and E. faecalis at 1.25 µL/mL, and MRSA and Bcereus at 0.625 µL/mL and (C) albicans at 0.312 µL/mL as the MIC. The antioxidant study of (EOs 1 + 2) with maximum inhibition percentage to DPPH assay was 84.02 ± 1.05 at 100 µg/mL, and minimal inhibition was 72.31 ± 0.63 at 5 µg/mL with IC50 value 4.69 ± 0.22 µg/mL, while ABTS assay extreme was 79.15 ± 2.14 µg/mL and minimal was 67 ± 1.34 with the IC50 value of 18.37 ± 0.15 µg/mL, in superoxide assay uppermost inhibition was 81.03 ± 0.27 µg/mL and lowest was at 65.16 ± 3.15 with the IC50 value 16.46 ± 0.54, and H2O2 radical scavenging activity, predominant value was 78.01 ± 0.47 and least was 64.1 ± 2.01 with IC50 15.58 ± 0.34. These comparative key diagnostic features and synergistic effect of multicomponent natural antimicrobials will provide profound intellect of ancient utility and further scientists to explore their multiple mechanistic modality and application in food and beverages industry.

Abstract Morphine, as one of the most important narcotic drugs, significantly affects the nervous system and increases euphoria, which raises the likelihood of its misuse. Therefore, its measurement is of great importance. In this work, a new electrochemical sensor based on a nanocomposite of CuS/g-C3N5/AgNPs was developed for modifying Screen printed carbon electrodes (SPCEs) and used for the measurement of morphine through cyclic voltammetry and differential pulse voltammetry. Various analytical methods initially characterized the nanocomposite. The prepared sensor, which also has an extensive surface area, achieved a detection limit of 0.01 µM for morphine in a concentration range of 0.05–100 µM at pH 7. Besides its excellent capability in measuring morphine in real samples, the sensor exhibits good stability, reproducibility, and repeatability. The presence of CuS, due to its excellent high surface area alongside silver nanoparticles, leads to an increase in the conductivity of the g-C3N5 modified electrode, resulting in an increased oxidative current of morphine at the surface of the prepared sensor. Therefore, measuring low concentrations of morphine with this sensor was made possible. Additionally, measuring morphine without interference from various species is a strong point of the electrochemical sensor for morphine detection, and combined with the simplicity and ease of the method, it allows for morphine measurements to be conducted in the shortest possible time.

Abstract Background Periodontal diseases, including gingivitis and periodontitis, are among the most prevalent oral health issues globally. They compromise the supportive structures of teeth and are influenced by both local and systemic factors. This study aimed to systematically assess the impact of systemic conditions on periodontal health in Malaysian population, addressing the gap in understanding these factors as potential risk factors. Methods A retrospective, non-interventional study was conducted using medical records from Hospital Universiti Sains Malaysia, Kelantan, from September 2019 to December 2022. The study included patients with periodontitis and systemic conditions. Data were analyzed using descriptive statistics, chi-squared tests, Fisher’s exact tests, Mann-Whitney U tests, Kruskal-Wallis tests, Spearman’s correlation, and logistic regression. Results Out of 600 records, 274 patients were included. The cohort was 51.8% male, with a median age of 51 years. Malays accounted for 92.3% of the sample. Hypertension and diabetes mellitus were the most common comorbid conditions. Severe periodontitis was significantly associated with age (45–64 years, p = 0.018) and Malay ethnicity (p = 0.011). Logistic regression revealed that age and ethnicity were significant predictors of periodontitis severity, with Malays being 12.5 times more likely to develop severe periodontitis. Conclusion Systemic conditions significantly influence periodontitis development and progression. Age and ethnicity are crucial predictors of periodontitis severity in the Malaysian population. Comprehensive risk assessment tools are necessary to incorporate a broader spectrum of risk factors for better management and prevention.

Background: The increasing prevalence of preterm birth in many societies has raised concerns. This study was conducted to determine the prevalence and risk factors associated with preterm birth in hospitals in Neyshabur, Eastern Iran. Methods: This cross-sectional study was conducted on 273 neonates born before 37 weeks of gestational age between 2017 and 2019 in the educational hospitals of Neyshabur. The infants were included in the research through convenience sampling. The research tool consisted of items addressing issues related to both mothers and newborns. Data analysis was performed using SPSS version 22, employing descriptive and analytical statistics, including chi-square and Fisher's exact tests. Results: The rate of preterm birth in Neyshabur over the three-year period was 2.85%. Bicornuate uterus (1.83%) and cervical insufficiency (1.46%) were the most observed uterine factors, while umbilical cord prolapse (1.83%) and placental adhesion (1.46%) were the most prevalent placental factors. Moreover, breech presentation (10.98%) was the most common co-occurring fetal factor in preterm birth. A significant difference was found between the length of pregnancy and the type of delivery (vaginal or cesarean section) (P=0.003). Conclusion: Due to the high prevalence of preterm birth in Neyshabur, it is recommended that mothers undergo periodic evaluations before and during pregnancy to identify and manage any accompanying problems or underlying conditions promptly.

Background: During times of crisis, the family caregivers of older adults need heightened attention to their own health. This study aimed to examine the impact of religious spiritual care training on the ability and spiritual health of family caregivers of older adults in Bushehr, Iran, during the COVID-19 pandemic. Materials and Methods: A field trial was conducted on 80 family caregivers of older adults referring to comprehensive health centers and the Comprehensive Rehabilitation Center in Bushehr by random sampling in 2022. Pouladi’s ability questionnaire and Polotzin's and Ellison's Spiritual Health Questionnaire were administered. The experimental group received spiritual religious training. Data were analyzed in SPSS version 19. Results: The mean change in the total ability scores was -0.87 ± 8.44 in the experimental group, while the control group exhibited a mean change of 0.27 ± 0.87. The mean change in the total spiritual health scores was 5.05 ± 11.12 in the experimental and -0.47 ± 0.96 in the control groups. Religious spiritual care training was effective in improving the physical-psychological ability of the research population (p=0.045), but it was not effective on the other areas of ability and overall ability (p>0.05). Religious spiritual intervention was effective in improving the existential dimension of spiritual health (p=0.001) and overall spiritual health (p=0.002), but it was not effective on the religious dimension of spiritual health (p>0.05) Conclusion: The effect of spiritual religious care training on physical-psychological ability and the existential dimension of spiritual health in the caregivers indicates the usefulness of the religious spiritual care model for strengthening the caregivers' physical, psychological, and care behavior. Further studies are recommended to explore the factors contributing to the other domains of ability and the religious dimension of spiritual health and to enhance caregivers' empowerment and spiritual health.

Background and purpose: Renal injury is a serious disorder that can be caused by some diseases or agents. Rosmarinic acid (RA) is a natural and safe compound with powerful antioxidant and anti-inflammatory properties. In this study, the ameliorative effects of RA were assayed in folic acid (FA)-induced renal injury by involving the SIRT1/NOX1 pathway. Experimental approach: Thirty-six male C57/BL6 mice were divided into 6 groups (n = 6) including control, vehicle, FA, RA, FA + RA 50, and FA + RA 100. After 10 days, blood urea nitrogen (BUN), creatinine, and oxidative stress were measured. The expression of SIRT1 and NOX1 proteins was evaluated by western blot. Also, histopathological alterations were assayed by H&E and PAS staining methods. Findings/Results: BUN and creatinine were significantly higher in the FA group compared to the control group; however, their levels decreased after RA treatment in both doses. A significant decrease was observed in swelling, necrosis, and desquamation of tubular epithelial cells in the FA + RA 50 and FA + RA 100 groups compared to the FA group. RA in the animals receiving FA increased SIRT1 expression and the levels of GSH and SOD compared to the FA group. RA in the animals receiving FA showed a significant decrease in NOX1 expression and MDA level compared to the FA group. Conclusion and implications: The findings declared that the administration of RA has positive effects against renal damage induced by FA. The effect might result from involvement in the SIRT1/NOX1 pathway and thereby attenuation of oxidative stress.

This article investigates the geometric and topologic of warped product submanifolds in Riemannian warped product Qεm×R{{\mathbb{Q}}}_{\varepsilon }^{m}\times {\mathbb{R}}. In this respect, we obtain the first Chen inequality that involves extrinsic invariants like the length of the warping functions and the mean curvature. This inequality involves two intrinsic invariants (sectional curvature and δ\delta -invariant). In addition, an integral bound is provided for the Bochner operator formula of compact warped product submanifolds in terms of the Ricci curvature gradient. We aim to apply this theory to many structures and obtain Dirichlet eigenvalues for problem applications. Some new results regarding the vanishing mean curvature are presented as a partial solution, and this can be considered for the well-known problem given by Chern.

Abstract Metabolic syndrome (MetS), comprising obesity, insulin resistance, hypertension, and dyslipidemia, increases the risk of type II diabetes mellitus and cardiovascular disease. This study aimed to identify the prevalence and determinants of specific clusters of the MetS components and tobacco consumption among older adults in Iran. The current study was conducted in the second stage of the Bushehr Elderly Health (BEH) program in southern Iran—a population-based cohort including 2424 subjects aged ≥ 60 years. Latent class analysis (LCA) was used to identify MetS and tobacco consumption patterns. Multinomial logistic regression was conducted to investigate factors associated with each MetS class, including sociodemographic and behavioral variables. Out of 2424 individuals, the overall percentage of people with one or more components of MetS or current tobacco use was 57.8% and 20.8%, respectively. The mean (SD) age of all participants was 69.3(6.4) years. LCA ascertained the presence of four latent classes: class 1 (“low risk”; with a prevalence of 35.3%), class 2 (“MetS with medication-controlled diabetes”; 11.1%), class 3 (“high risk of MetS and associated medication use”; 27.1%), and class 4 (“central obesity and treated hypertension”; 26.4%). Compared to participants with a body mass index (BMI)

Abstract The urgent need for sustainable agriculture has intensified the search for environmentally friendly alternatives to chemical herbicides. This study investigates the herbicidal potential of siderophores produced by Amycolatopsis lurida strain 407, focusing on its effects on the growth of ryegrass and redroot weeds. Strain 407 exhibited two distinct colony morphologies—red and white—when cultured under varying environmental conditions. The cell-free culture filtrate (CFCF) from both colony types significantly inhibited the growth of ryegrass and redroot. The concentration of siderophore produced in the iron-deficient medium was measured to be 613.4 ppm for 407 red and 388.5 ppm for 407 white, which indicates significant iron chelating activity. This study also showed a direct relationship between the presence of siderophore in plant culture medium and reduced growth. Also, analysis of fractions of the aqueous phase resulting from column chromatography revealed that all fractions from the 407 red reduced ryegrass shoot length by up to 45% and root length by 83–86%, while redroot seedling length decreased by up to 36%. Fractions from 407 white completely inhibited germination or reduced ryegrass root length by up to 94% and redroot seedling length by 52%. Fractions F4 W to F7 W and F2 R to F8 R, which showed iron chelating activity were most effective in reducing plant growth, suggesting that there are metabolites, alone or in company with siderophores, synergistically do herbicidal activity. The innovative application of siderophores as bioherbicide presents a promising environmentally friendly alternative to chemical herbicides.

Abstract Synthetic dyes, such as Alizarin Red S, contribute significantly to environmental pollution. This study investigates the biosorption potential of Alhagi maurorum biosorbent for the removal of Alizarin Red S (ARS) from aqueous solutions. Fourier transform infrared spectroscopy (FTIR) was used to analyze the biosorbent’s adsorption sites. Various parameters were optimized to maximize dye adsorption. An optimal removal efficiency of 82.26% was attained by employing 0.9 g of biosorbent with a 25 ppm dye concentration at pH 6 and 60 °C over 30 min. The data were modeled using various isothermal and kinetic models to understand the adsorption behavior. Thermodynamic parameters indicated that the adsorption process was spontaneous and endothermic. The pseudo-second-order kinetic model best described the data, indicating chemisorption as the rate-limiting step. The data matched best to the Langmuir model, indicating that the adsorption occurs as a monolayer on uniform surfaces with a finite number of binding sites. The model showed a strong correlation (R² = 0.991) and a maximum adsorption capacity (qmax) of 8.203 mg/g. Principal component analysis (PCA) identified temperature as the dominant factor, with the primary component, PC1 capturing 100% of its effect. The mechanisms involved in ARS biosorption on A. maurorum include electrostatic interactions, hydrogen bonding, hydrophobic interactions, dipole-dipole interactions, and π-π stacking. Alhagi maurorum showed promising potential for biosorbing toxic dyes from contaminated water, suggesting further investigation for practical applications.

Abstract Introduction Stroke is one of the most devastating complications of sickle cell disease (SCD). Transcranial Doppler Imaging (TCDI) is the least invasive screening method to predict patients at risk for developing stroke in the disease. After a 10-year follow-up, we longitudinally assessed the TCDI in children with SCD without neurological symptoms. Methods 25 out of 43 pediatric patients with SCD studied 10-year previously were recruited. The remaining 18 patient were not available for follow-up, but their initial data are presented for comparison. TCDI scanning was carried out using a phased-array transducer of 1–3 MHz through the trans-temporal window. Peak systolic velocity (PSV), end-diastolic velocity (EDV), time-averaged mean of the maximum velocity (TAMMV), resistive index (RI), and pulsatility index (PI) were obtained in the anterior and posterior Circle of Willis vessels. Results The highest initial and follow-up TAMMV (mean ± SD) were: 77.3 ± 20.9 and 71.6 ± 9.9 in the t-ICA, 94.3 ± 25.8 and 82 ± 18.2 in the MCA, 76.6 ± 25.6 and 70.6 ± 10.7 in the ACA, and 59.1 ± 15.8 and 63.9 ± 8.5 in the PCA, respectively. There was no statistically significant difference between initial and follow-up SCD data for all vascular parameters in all vessels on each side (P > 0.05) except for RI and PI (P

Papaya (Carica papaya) fruit has a short shelf life and suffers significant postharvest losses due to climacteric, instant ripening and perishable nature. Pre-storage hot water treatment (HWT) has significant potential to delay decay incidence in horticultural commodities. The present study was designed to evaluate effectiveness of HWT on ‘Red lady’ papaya fruit for minimizing the fruit decay and maintaining the quality during storage. Physiologically mature unripe ‘Red lady’ papaya fruit harvested from a commercial papaya orchard. Selected fruits were treated at three different hot water temperatures (50 °C, 52 °C and 54 °C) along with a control (room temp) for 10 minutes thereafter stored at 12 °C and 85–90% RH for four weeks. Different sensory evaluation, fruit quality scales and enzymatic activities were observed at weekly intervals. Fruit stored after 54 °C HWT showed lower weight loss (4.63%), decay incidence (5.1%) and higher fruit firmness (12.92 N) relative to control. Fruit treated at 52 °C HWT improved the fruit acidity, but total soluble solids decreased than control. The results revealed that ascorbic acid (33.33 mg/100 g), total phenolic contents (35 mg/g FW) total antioxidants (47.50% DPPH inhibition), and antioxidative enzyme activities were improved by 52 °C HWT. It is concluded that pre storage papaya fruit treatment (HWT @ 52 °C) preserves the quality of fruit throughout storage on the other hand at 54 °C HWT helps in decreased fruit decaying. It can be summarized that papaya fruit can be treated with hot water in the range of 52–54 °C to support the fruit shelf life and suppress fruit decay along with maintaining fruit quality.Practical applications: Papaya (Carica papaya L.) is a climacteric fruit which is highly susceptible to fungal attacks after harvest. Due to its perishable nature, it becomes decayed and huge amount of highly nutritious and medicinally important fruit is wasted. Recently people are more concerned about food safety which limits the use of fungicides for controlling the fruit decay in papaya. However, heat treatment has tremendous potential to control microbial decay, and keep food commodities safe from hazardous chemicals. In this study, the hot water treatment has successfully suppressed the fruit decay and maintained fruit quality of papaya in the course of entire period of cold storage. These findings indicated that use of hot water treatment is a practical approach for increasing the shelf life of papaya fruit.

Abstract Background Cancer is the leading cause of disease-related mortality in children. Causes of leukemia, the most common form, are largely unknown. Growing evidence points to an origin in-utero, when global redistribution of DNA methylation occurs driving tissue differentiation. Methods Epigenome-wide DNA methylation was profiled in surrogate (blood) and target (bone marrow) tissues at birth, diagnosis, remission and relapse of pediatric pre-B acute lymphoblastic leukemia (pre-B ALL) patients. Double-blinded analyses was performed between prospective cohorts extending from birth to diagnosis and retrospective studies backtracking from clinical disease to birth. Validation was carried out using independent technologies and populations. Results The imprinted and immuno-modulating VTRNA2-1 was hypermethylated (FDR

Abstract Tamarix tetragyna is a plant grows in Mediterranean area and some Arab countries. It possesses numerous medicinal values. Purpose of our study is to explore biological activity of tamarix tetragyna extracts of both leaves and stem with investigating their phytochemical composition. The investigated extracts’ phyto-constituent composition was determined using gas chromatographic-mass spectrometric method. In addition, in vitro cytoxicity activity versus cancer cell lines such MCF-7, HepG-2, HCT-116, and A-549 was examined by MTT assay method, together with exploring its apoptosis effect by flow cytometry and western blot analysis techniques. Moreover, some phytochemical compounds were identified, and in-silico evaluated against anticancer molecular targets. Plant extracts showed good cytotoxic activity against both A-549 and HCT-116 cancer cell lines. With an IC50 value of 23.90 µg/ml that led to apoptosis and G2/M-phase arrest in A-549 cells, cytotoxicity data demonstrate leaves’ extract effectiveness against these cells. Upon GC-MS analysis, it revealed presence of some bioactive components such as Stigmast-5-en-3-ol and 2-methoxy-4-vinyl phenol, which are known for their cytotoxic activity. Our findings suggest that methanolic extracts of Tamarix tetragyna parts may have potential therapeutic uses as anticancer against A-549 cells, which opens up further avenues for investigation into its industrial applications.

Abstract Influenza poses a significant threat to global health, with the ability to cause severe epidemics and pandemics. The polymerase basic protein 2 (PB2) of the influenza virus plays a crucial role in the viral replication process, making the CAP-binding domain of PB2 an attractive target for antiviral drug development. This study aimed to identify and evaluate potential inhibitors of the influenza polymerase PB2 CAP-binding domain using computational drug discovery methods. We employed a comprehensive computational approach involving virtual screening, molecular docking, and 500 ns molecular dynamics (MD) simulations. Compounds were selected from the Diverse lib database and assessed for their binding affinity and stability in interaction with the PB2 CAP-binding domain. The study utilized the generalized amber force field (GAFF) for MD simulations to further evaluate the dynamic behaviour and stability of the interactions. Among the screened compounds, compounds 1, 3, and 4 showed promising binding affinities. Compound 4 demonstrated the highest binding stability and the most favourable free energy profile, indicating strong and consistent interaction with the target domain. Compound 3 displayed moderate stability with dynamic conformational changes, while Compound 1 maintained robust interactions throughout the simulations. Comparative analyses of these compounds against a control compound highlighted their potential efficacy. Compound 4 emerged as the most promising inhibitor, with substantial stability and strong binding affinity to the PB2 CAP-binding domain. These findings suggest that compound 4, along with compounds 1 and 3, holds the potential for further development into effective antiviral agents against influenza. Future studies should focus on experimental validation of these compounds and exploration of resistance mechanisms to enhance their therapeutic utility.

Abstract Background Type 2 diabetes mellitus (T2DM) was one of the most prevalent comorbidities among patients with coronavirus disease 2019 (COVID-19). Interactions between different metabolic parameters contribute to the susceptibility to the virus; thereby, this study aimed to rank the importance of clinical and laboratory variables as risk factors for COVID-19 or as protective factors against it by applying machine learning methods. Method This study is a retrospective cohort conducted at a single center, focusing on a population with T2DM. The patients attended the Yazd Diabetes Research Center in Yazd, Iran, from February 20, 2020, to October 21, 2020. Clinical and laboratory data were collected within three months before the onset of the COVID-19 pandemic in Iran. 59 patients were infected with COVID-19, while 59 were not. The dataset was split into 70% training and 30% test sets. Principal Component Analysis (PCA) was applied to the data. The most important components were selected using a ‘sequential feature selector’ and scored by a Linear Discriminant Analysis model. PCA loadings were then multiplied by the PCs’ scores to determine the importance of the original variables in contracting COVID-19. Results HDL-C, followed by eGFR, showed a strong negative correlation with the risk of contracting the virus. Higher levels of HDL-C and eGFR offer protection against COVID-19 in the T2DM population. But, the ratio of BUN to creatinine did not show any correlation. Conversely, the AIP, TyG index and TG showed the most positive correlation with susceptibility to COVID-19 in such a way that higher levels of these factors increase the risk of contracting the virus. The positive correlation of diastolic BP, TyG-BMI index, MAP, BMI, weight, TC, FPG, HbA1C, Cr, systolic BP, BUN, and LDL-C with the risk of COVID-19 decreased, respectively. Conclusion The atherogenic index of plasma, triglyceride glucose index, and triglyceride levels are the most significant risk factors for COVID-19 contracting in individuals with T2DM. Meanwhile, high-density lipoprotein cholesterol is the most protective factor.

Abstract Background Military personnel often face unique challenges in maintaining optimal oral health. This study investigated the oral health status, caries experience, and associated factors among a sample of Iranian soldiers, employing a structural equation modeling (SEM) approach to explore the complex interplay of socioeconomic and behavioral determinants. Methods A cross-sectional study was conducted among 658 male soldiers aged 18–30 years from three military barracks in Fars province, Iran. Data were collected through a structured instrument and clinical oral examinations. The study employs the DMFT index, which measures caries experience based on decayed, missing, and filled teeth, along with the Simplified Oral Hygiene Index (OHI-S) to assess overall oral health status. Structural equation modeling was employed to analyze the complex relationships between socioeconomic factors, oral health behaviors, and oral health outcomes. Results The mean DMFT score was 3.57 ± 5.91, and the mean OHIS score was 0.56 ± 1.42. SEM analysis revealed that socioeconomic status (SES) indirectly influenced DMFT and oral hygiene scores, mediated by drug use, oral hygiene practices, dietary sugar consumption, and dental visit frequency. Lower toothbrushing frequency was significantly associated with higher DMFT (Estimate = -0.064, p

The Consumer-Oriented Trusted Service Manager (CO-TSM) model has been recognised as a significant advancement in managing applications on Near Field Communication (NFC)-enabled mobile devices and multi-application smart cards. Traditional Trusted Service Manager (TSM) models, while useful, often result in market fragmentation and limit widespread adoption due to their centralised control mechanisms. The CO-TSM model addresses these issues by decentralising management and offering greater flexibility and scalability, making it more adaptable to the evolving needs of embedded systems, particularly in the context of the Internet of Things (IoT) and Radio Frequency Identification (RFID) technologies. This paper provides a comprehensive analysis of the CO-TSM model, highlighting its application in various technological domains such as smart cards, HCE-based NFC mobile phones, TEE-enabled smart home IoT devices, and RFID-based smart supply chains. By evaluating the CO-TSM model’s architecture, implementation challenges, and practical deployment scenarios, this paper demonstrates how CO-TSM can overcome the limitations of traditional TSM approaches. The case studies presented offer practical insights into the model’s adaptability and effectiveness in real-world scenarios. Through this examination, the paper aims to underscore the CO-TSM model’s role in enhancing scalability, flexibility, and user autonomy in secure embedded device management, while also identifying areas for future research and development.

Abstract Background Cerebrovascular accidents (CVAs), or strokes, are major global health concerns associated with oxidative stress, inflammation, and gastrointestinal complications. This study aimed to explore the impact of postbiotic supplementation in CVA patients, specifically in terms of oxidative stress, inflammation, and clinical outcomes, as an alternative to probiotics with potential advantages. Method A prospective, single-center, randomized, controlled trial was conducted with 120 CVA patients in Iran. These patients were admitted to the ICU to assess the severity of their strokes. Patients were randomly assigned to receive either postbiotic supplementation (n = 60) or a placebo (n = 60). Various biomarkers related to oxidative stress, inflammation, and clinical outcomes were assessed. Data on demographic characteristics, nosocomial infections, and laboratory measurements were collected. Gut microbiota analysis was also performed on fecal samples. Results After the 7-day intervention, postbiotic supplementation resulted in significant improvements in inflammatory markers, oxidative stress, and a reduced incidence of pneumonia compared with those in the control group, with the postbiotic group demonstrating notable decreases in the serum IL-1β levels (-1.79; 95% CI: = -2.9 to -0.64, p = 0.002 ), MDA levels (-30.5; 95% CI: -54.8 to -6.1, p = 0.015), Hs-CRP levels (-0.67; 95% CI:-1.1 to -0.26 mg/dl, p = 0.001) and TAC levels (62.5; 95%CI: 34.1 to 90.9, p

Abstract The widespread use of pesticides poses significant threats to both environmental and human health, primarily due to their potential toxic effects. The study investigated the cardiovascular toxicity of selected pesticides, focusing on their interactions with Toll-like receptor 4 (TLR4), an important part of the innate immune system. Using computational tools such as molecular docking, molecular dynamics (MD) simulations, principal component analysis (PCA), density functional theory (DFT) calculations, and ADME analysis, this study identified C160 as having the lowest binding affinity (-8.2 kcal/mol), followed by C107 and C165 (-8.0 kcal/mol). RMSD, RMSF, Rg, and hydrogen bond metrics indicated the formation of stable complexes between specific pesticides and TLR4. PCA revealed significant structural changes upon ligand binding, affecting stability and flexibility, while DFT calculations provided information about the stability, reactivity, and polarity of the compounds. ADME studies highlighted the solubility, permeability, and metabolic stability of C107, C160, and C165, suggesting their potential for bioavailability and impact on cardiovascular toxicity. C107 and C165 exhibit higher bioactivity scores, indicating favourable absorption, metabolism, and distribution properties. C165 also violated rule where molecular weight is greater than 500 g/mol. Further, DFT and NCI analysis of post MD conformations confirmed the binding of ligands at the binding pocket. The analysis shed light on the molecular mechanisms of pesticide-induced cardiovascular toxicity, aiding in the development of strategies to mitigate their harmful effects on human health.