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A new phlebovirus variant was isolated from an acute febrile patient in Chanchamayo, Peru. Genome characterization and p-distance analyses based on complete open reading frames revealed that the virus is probably a natural reassortant of the Echarate virus (large and small segments) with a yet-unidentified phlebovirus (M segment).

Objective. To develop and validate a cell suspension method using Vero 76 cells for culturing Zika virus (ZIKV) based on infection of detached freshly seeded cells. Material and methods. Three different multiplicities of ZIKV infection were used to develop and compare this novel method to the standard confluent cell monolayer method. In addition, we preliminary validated the cell suspension method using well-characterized ZIKV positive and negative clinical samples. The standard confluent cell monolayer method was used as the reference method, and viral isolation was confirmed by a ZIKV-specific RT-PCR. We estimated the sensitivity, and its 95% confidence intervals, for the cell suspension method. Also, a technical comparison of the cell suspension method against the cell monolayer method was performed. Results. Our findings suggest that both the viral load and replication of ZIKV were comparable between both monolayer- and suspension-infection methods. Although both methods were suitable for culturing and isolating ZIKV, the cell suspension method was easier, cheaper, and quicker as well as a sensitive isolation technique. The cell suspension method was significantly more sensitive in detecting Zika in inconclusive cases by RT-PCR, with a fourfold increase compared to the confluent cell monolayer method. Conclusion. The cell suspension method has the potential to be an effective method for cultivating and isolating ZIKV and its application is potentially useful in both research and clinical settings.

Objetivo. Describir la experiencia inicial en ablación de arritmias cardiacas empleando mapeo 3D en el Instituto Nacional Cardiovascular INCOR (Lima-Perú). Materiales y métodos. Estudio descriptivo, retrospectivo, donde se recolectaron datos de historias clínicas de todos los pacientes en los cuales se realizó ablación empleando mapeo 3D, desde julio de 2017 a diciembre de 2019. Este procedimiento se realizó a pacientes sintomáticos y refractarios a terapia antiarrítmica. Resultados. Se recolectaron datos de 123 pacientes (mediana de edad: 46 años, 64,2% varones), con una mediana del tiempo de enfermedad de 6 años. Entre las arritmias tratadas 19% tuvieron fibrilación auricular; 17,5% taquicardia auricular; 17,5% arritmias ventriculares idiopáticas; 16,6% síndrome de Wolf Parkinson White/taquicardia por reentrada auriculoventricular; 11,1% arritmias ventriculares del sistema de conducción His-Purkinje; 9,5% taquicardia ventricular asociada con cicatriz; 6,4% flutter atrial y 2,4% taquicardia intranodal. La mediana del tiempo de fluoroscopía fue de 26 min. La ablación tuvo éxito agudo en el 95,9% de los casos; las complicaciones agudas se observaron en 4,8%, y la sobrevida libre de recurrencias durante el primer año de seguimiento fue de 74%. Conclusiones. Nuestra experiencia en ablación de arritmias cardiacas empleando mapeo 3D presentó una alta frecuencia de ablación exitosa aguda y baja frecuencia de complicaciones, así como una sobrevida libre de recurrencias del 74%, estimada al año de seguimiento.

Aim: The SAMe-TT2R2 score helps identify patients with atrial fibrillation (AF) likely to have poor anticoagulation control during anticoagulation with vitamin K antagonists (VKA) and those with scores >2 might be better managed with a target-specific oral anticoagulant (NOAC). We hypothesized that in clinical practice, VKAs may be prescribed less frequently to patients with AF and SAMe-TT2R2 scores >2 than to patients with lower scores. Methods and results: We analyzed the Phase III dataset of the Global Registry on Long-Term Oral Antithrombotic Treatment in Patients with Atrial Fibrillation (GLORIA-AF), a large, global, prospective global registry of patients with newly diagnosed AF and ≥1 stroke risk factor. We compared baseline clinical characteristics and antithrombotic prescriptions to determine the probability of the VKA prescription among anticoagulated patients with the baseline SAMe-TT2R2 score >2 and ≤ 2. Among 17,465 anticoagulated patients with AF, 4,828 (27.6%) patients were prescribed VKA and 12,637 (72.4%) patients an NOAC: 11,884 (68.0%) patients had SAMe-TT2R2 scores 0-2 and 5,581 (32.0%) patients had scores >2. The proportion of patients prescribed VKA was 28.0% among patients with SAMe-TT2R2 scores >2 and 27.5% in those with scores ≤2. Conclusions: The lack of a clear association between the SAMe-TT2R2 score and anticoagulant selection may be attributed to the relative efficacy and safety profiles between NOACs and VKAs as well as to the absence of trial evidence that an SAMe-TT2R2-guided strategy for the selection of the type of anticoagulation in NVAF patients has an impact on clinical outcomes of efficacy and safety. The latter hypothesis is currently being tested in a randomized controlled trial. Clinical trial registration: URL: https://www.clinicaltrials.gov//Unique identifier: NCT01937377, NCT01468701, and NCT01671007.

Esta es una investigación cualitativa de tipo exploratorio, que tiene como objetivo examinar las Representaciones sociales acerca de la adopción en parejas sin hijos y con posibilidades de adoptar, para lo cual se aplicará una entrevista semiestructurada a 50 parejas, además de una ficha de caracterización sociodemográfica elaborada para esta investigación. Se concluye que las parejas tienen una representación sesgada de la adopción, y prejuicios respecto a la edad, la salud, la historia de los niños y niñas, que determinan las resistencias y representaciones respecto a la adopción. Asimismo, las parejas anteponen el proyecto de vida personal, la estabilidad económica, dificultades en los procesos adoptivos, y supuestas preferencias por familias extranjeras, como criterios que dan forma a la resistencia a adoptar, aun cuando identifican que la adopción es un proceso necesario en la sociedad que debe ser fortalecido y asumido por más personas.

During April–June 2014 in a malaria-endemic rural community close to the city of Iquitos in Peru, we detected evidence of Guaroa virus (GROV) infection in 14 febrile persons, of whom 6 also had evidence of Plasmodium vivax malaria. Cases were discovered through a long-term febrile illness surveillance network at local participating health facilities. GROV cases were identified by using a combination of seroconversion and virus isolation, and malaria was diagnosed by thick smear and PCR. GROV mono-infections manifested as nonspecific febrile illness and were clinically indistinguishable from GROV and P. vivax co-infections. This cluster of cases highlights the potential for GROV transmission in the rural Peruvian Amazon, particularly in areas where malaria is endemic. Further study of similar areas of the Amazon may provide insights into the extent of GROV transmission in the Amazon basin.

We describe an Oropouche orthobunyavirus infection in a women 28 years of age in Colombia. We confirmed the diagnosis by viral isolation, quantitative reverse transcription PCR, and phylogenetic analysis of the small, medium, and large genomic segments. The virus is related to a strain isolated in Ecuador in 2016.

Introducción. El presente artículo resume la guía de práctica clínica (GPC) para el manejo de pacientes con fibrilación auricular (FA) en el Seguro Social del Perú (EsSalud). Objetivo. Proveer recomendaciones clínicas basadas en evidencia para el manejo de pacientes con FA en EsSalud. Métodos. Se conformó un grupo elaborador (GEG) que incluyó médicos especialistas y metodólogos. El GEG formuló 9 preguntas clínicas a ser respondidas por la presente GPC. Se realizó búsquedas sistemáticas de revisiones sistemáticas y ,cuando fue considerado pertinente, estudios primarios en Medline durante el 2017. Se seleccionó la evidencia para responder cada una de las preguntas clínicas planteadas. La certeza de la evidencia fue evaluada usando la metodología Grading of Recommendations Assessment, Development, and Evaluation (GRADE). En reuniones de trabajo periódicas, el GEG usó la metodología GRADE para revisar la evidencia y formular las recomendaciones, los puntos de buenas prácticas clínicas y los flujogramas de la GPC. Finalmente, la GPC fue aprobada con Resolución N° 91 – IETSI – ESSALUD – 2018. Resultados. La presente GPC abordó 9 preguntas clínicas, divididas en tres temas: prevención de eventos tromboembólicos, estrategias de control y manejo de FA en emergencias. En base a dichas preguntas se formularon 12 recomendaciones (6 recomendaciones fuertes y 6 recomendaciones condicionales), 29 puntos de buena práctica clínica, y 2 algoritmos. Conclusión. El presente artículo resume la metodología y las conclusiones basadas en evidencias de la GPC para manejo de pacientes con FA en EsSalud.

Zika virus (ZIKV) emerged and spread rapidly in South American countries during 2015. Efforts to diagnose ZIKV infection using serological tools were challenging in dengue-endemic areas because of antigenic similarities between both viruses. Here, we assessed the performance of an in-house developed IgM antibody capture enzyme-linked immunosorbent assay (MAC-ELISA) and the plaque reduction neutralization test (PRNT) to diagnose ZIKV infection. Acute and convalescent paired serum samples from 51 patients who presented with clinical symptoms suggestive of an arbovirus illness in dengue-endemic areas of Honduras, Venezuela, Colombia and Peru were used in the assessment. Samples were tested for ZIKV, dengue and chikungunya virus using a variety of laboratory techniques. The results for the ZIKV-RNA screening and seroconversion detected by the microneutralization test were used to construct a composite reference standard. The overall sensitivity and specificity for the MAC-ELISA were 93.5% and 100.0%, respectively. Contrastingly, the overall sensitivity and specificity for the PRNT were 96.8% and 95.0%, respectively. Restricting the analysis according to IgM or neutralizing antibodies against dengue, the performances of both serological assays were adequate. The findings of this study reveal that the MAC-ELISA and PRNT would provide initial reliable laboratory diagnostic assays for ZIKV infection in dengue-endemic areas.

Despite the lack of evidence for symptomatic human infection with Maguari virus (MAGV), its close relation to Cache Valley virus (CVV), which does infect humans, remains a concern. We sequenced the complete genome of a MAGV-like isolate (OBS6657) obtained from a febrile patient in Pucallpa, Ucayali, Peru, in 1998. To facilitate its classification, we generated additional full-length sequences for the MAGV prototype strain, 3 additional MAGV-like isolates, and the closely related CVV (7 strains), Tlacotalpan (1 strain), Playas (3 strains), and Fort Sherman (1 strain) viruses. The OBS6657 isolate is similar to the MAGV prototype, whereas 2 of the other MAGV-like isolates are located on a distinct branch and most likely warrant classification as a separate virus species and 1 is, in fact, a misclassified CVV strain. Our findings provide clear evidence that MAGV can cause human disease.

While studying respiratory infections in Peru, we identified Venezuelan equine encephalitis virus (VEEV) in a nasopharyngeal swab, indicating that this alphavirus can be present in human respiratory secretions. Because VEEV may be infectious when aerosolized, our finding is relevant for the management of VEEV-infected patients and for VEEV transmission studies.

ABSTRACT Mayaro virus (MAYV), Venezuelan equine encephalitis virus (VEEV), and chikungunya virus (CHIKV) are vector-borne alphaviruses that cocirculate in South America. Human infections by these viruses are frequently underdiagnosed or misdiagnosed, especially in areas with high dengue virus endemicity. Disease may progress to debilitating arthralgia (MAYV, CHIKV), encephalitis (VEEV), and death. Few standardized serological assays exist for specific human alphavirus infection detection, and antigen cross-reactivity can be problematic. Therefore, serological platforms that aid in the specific detection of multiple alphavirus infections will greatly expand disease surveillance for these emerging infections. In this study, serum samples from South American patients with PCR- and/or isolation-confirmed infections caused by MAYV, VEEV, and CHIKV were examined by using a protein microarray assembled with recombinant capsid, envelope protein 1 (E1), and E2 from nine New and Old World alphaviruses. Notably, specific antibody recognition of E1 was observed only with MAYV infections, whereas E2 was specifically targeted by antibodies from all of the alphavirus infections investigated, with evidence of cross-reactivity to E2 of o’nyong-nyong virus only in CHIKV-infected patient serum samples. Our findings suggest that alphavirus structural protein microarrays can distinguish infections caused by MAYV, VEEV, and CHIKV and that this multiplexed serological platform could be useful for high-throughput disease surveillance. IMPORTANCE Mayaro, chikungunya, and Venezuelan equine encephalitis viruses are closely related alphaviruses that are spread by mosquitos, causing diseases that produce similar influenza-like symptoms or more severe illnesses. Moreover, alphavirus infection symptoms can be similar to those of dengue or Zika disease, leading to underreporting of cases and potential misdiagnoses. New methods that can be used to detect antibody responses to multiple alphaviruses within the same assay would greatly aid disease surveillance efforts. However, possible antibody cross-reactivity between viruses can reduce the quality of laboratory results. Our results demonstrate that antibody responses to multiple alphaviruses can be specifically quantified within the same assay by using selected recombinant protein antigens and further show that Mayaro virus infections result in unique responses to viral envelope proteins.

While studying respiratory infections of unknown etiology we detected Saffold virus in an oropharyngeal swab collected from a two-year-old female suffering from diarrhea and respiratory illness. The full viral genome recovered by deep sequencing showed 98% identity to a previously described Saffold strain isolated in Japan. Phylogenetic analysis confirmed the Peruvian Saffold strain belongs to genotype 3 and is most closely related to strains that have circulated in Asia. This is the first documented case report of Saffold virus in Peru and the only complete genomic characterization of a Saffold-3 isolate from the Americas.

Our genetic analyses of uncharacterized bunyaviruses isolated in Peru identified a possible reassortant virus containing small and large gene segment sequences closely related to the Caraparu virus and a medium gene segment sequence potentially derived from an unidentified group C orthobunyavirus. Neutralization tests confirmed serologic distinction among the newly identified virus and the prototype and Caraparu strains. This virus, named Itaya, was isolated in 1999 and 2006 from febrile patients in the cities of Iquitos and Yurimaguas in Peru. The geographic distance between the 2 cases suggests that the Itaya virus could be widely distributed throughout the Amazon basin in northeastern Peru. Identification of a new Orthobunyavirus species that causes febrile disease in humans reinforces the need to expand viral disease surveillance in tropical regions of South America.

We conducted phylogeographic modeling to determine the introduction and spread of Guaroa virus in South America. The results suggest a recent introduction of this virus into regions of Peru and Bolivia over the past 60–70 years and emphasize the need for increased surveillance in surrounding areas.

During 2010–2013, we recruited 16 persons with confirmed Mayaro virus infection in the Peruvian Amazon to prospectively follow clinical symptoms and serologic response over a 12-month period. Mayaro virus infection caused long-term arthralgia in more than half, similar to reports of other arthritogenic alphaviruses.

To better describe the genetic diversity of hantaviruses associated with human illness in South America, we screened blood samples from febrile patients in Chapare Province in central Bolivia during 2008–2009 for recent hantavirus infection. Hantavirus RNA was detected in 3 patients, including 1 who died. Partial RNA sequences of small and medium segments from the 3 patients were most closely related to Andes virus lineages but distinct (1 hantaviruses; the highest prevalence was among agricultural workers. Because of the high level of human exposure to hantavirus strains and the severity of resulting disease, additional studies are warranted to determine the reservoirs, ecologic range, and public health effect of this novel strain of hantavirus.

Este articulo muestra la importancia que la tradición jurídica y la organización de un sistema judicial; reclutamiento, garantías de inmovilidad, presupuesto y prescripciones salariales, pueden tener en la mayor o menor independencia del judicial. Del mismo modo, presenta cómo los estudios que sobre independencia judicial han sido realizados en Colombia han privilegiado a la Corte Constitucional y en menor medida a la Corte Suprema de Justicia. Finalmente el estudio concluye que pese a toda una serie de garantías normativas que buscan preservar la independencia de los jueces en Colombia, ésta es muy variable, y depende en últimas de la voluntad del Ejecutivo.

In 2008, dengue virus serotype 4 (DENV-4) emerged in northeastern Peru, causing a large outbreak and displacing DENV-3, which had predominated for the previous 6 years. Phylogenetic analysis of 2008 and 2009 isolates support their inclusion into DENV-4 genotype II, forming a lineage distinct from strains that had previously circulated in the region.

After a human rabies outbreak in southeastern Peru, we collected bats to estimate the prevalence of rabies in various species. Among 165 bats from 6 genera and 10 species, 10.3% were antibody positive; antibody prevalence was similar in vampire and nonvampire bats. Thus, nonvampire bats may also be a source for human rabies in Peru.

En Colombia se asiste a la explosión del número de denuncias por faltas de todo tipo en contra de magistrados de la Corte Suprema de Justicia y del Consejo Superior de la Judicatura. También, se acrecienta la sensación de que los jueces cuentan con suficientes arreglos que garantizan su independencia, sin que a la par se asegure que estos respondan por sus acciones y omisiones. El presente documento busca mostrar cómo pese a la existencia de un cuerpo normativo que busca asegurar que los altos jueces rindan cuentas, en la práctica estos mecanismos se muestran poco eficaces. La fractura al sistema de rendición de cuentas puede rastrearse en la deficiente gestión del ente que por ley controla a los togados: la Comisión de Investigación y Acusación de la Cámara de Representantes.

Since Venezuelan equine encephalitis virus (VEEV) was isolated in Peru in 1942, >70 isolates have been obtained from mosquitoes, humans, and sylvatic mammals primarily in the Amazon region. To investigate genetic relationships among the Peru VEEV isolates and between the Peru isolates and other VEEV strains, a fragment of the PE2 gene was amplified and analyzed by single-stranded conformation polymorphism. Representatives of seven genotypes underwent sequencing and phylogenetic analysis. The results identified four VEE complex lineages that cocirculate in the Amazon region: subtypes ID (Panama and Colombia/Venezuela genotypes), IIIC, and a new, proposed subtype IIID, which was isolated from a febrile human, mosquitoes, and spiny rats. Both ID lineages and the IIID subtype are associated with febrile human illness. Most of the subtype ID isolates belonged to the Panama genotype, but the Colombia/Venezuela genotype, which is phylogenetically related to epizootic strains, also continues to circulate in the Amazon basin.

Mayaro virus (MAYV), an alphavirus similar to chikungunya virus (CHIKV), causes an acute debilitating disease which results in the development of long-term arthralgia in more than 50% of infected individuals. Currently, the immune response and its role in the development of MAYV-induced persistent arthralgia remain unknown. In this study, we evaluated the immune response of individuals with confirmed MAYV infection in a one-year longitudinal study carried out in Loreto, Peru. We report that MAYV infection elicits robust immune responses that result in the development of a strong neutralizing antibody response and the secretion of pro-inflammatory immune mediators. The composition of these inflammatory mediators, in some cases, differed to those previously observed for CHIKV. Key mediators such as IL-13, IL-7 and VEGF were strongly induced following MAYV infection and were significantly increased in subjects that eventually developed persistent arthralgia. Although a strong neutralizing antibody response was observed in all subjects, it was not sufficient to prevent the long-term outcomes of MAYV infection. This study provides initial immunologic insight that may eventually contribute to prognostic tools and therapeutic treatments against this emerging pathogen.

La primera generación formada con el plan curricular por competencias, implementado en el 2006 y la última generación del plan curricular tradicional, con 50 años de antigüedad, fueron admitidos simultáneamente en el Internado de pregrado en junio de 2010. Oportunidad para evaluar ambas generaciones, con el objetivo: Determinar cuál de los modelos: Competencias o Tradicional, favorecen más las competencias que integran el perfil de egreso; a través de un estudio comparativo donde se aplicó un Examen Clínico Objetivo estructurado (ECOE) a 56 Médicos Internos de Pregrado (MIP), 28 de cada generación para valorar la competencia clínica con pacientes estandarizados en 18 estaciones con lista de cotejo y rúbrica. También se administró un cuestionario a 266 MIP y 365 personas que acompañan su formación para conocer la relación entre la administración educativa del plan curricular por competencias y el desempeño profesional de los MIP. El procesamiento de la información se realizó con nivel de significancia de p

Oropouche (ORO) virus, a member of the Simbu serogroup, is one of the few human pathogens in the Orthobunyavirus genus in the family Bunyaviridae. Genetic analyses of ORO-like strains from Iquitos, Peru, identified a novel reassortant containing the S and L segments of ORO virus and the M segment of a novel Simbu serogroup virus. This new pathogen, which we named Iquitos (IQT) virus, was first isolated during 1999 from a febrile patient in Iquitos, an Amazonian city in Peru. Subsequently, the virus was identified as the cause of outbreaks of "Oropouche fever" during 2005 and 2006 in Iquitos. In addition to the identification of 17 isolates of IQT virus between 1999 and 2006, surveys for neutralizing antibody among Iquitos residents revealed prevalence rates of 14.9% for ORO virus and 15.4% for IQT virus. Limited studies indicate that prior infection with ORO virus does not seem to protect against disease caused with the IQT virus infection. Identification of a new Orthobunyavirus human pathogen in the Amazon region of Peru highlights the need for strengthening surveillance activities and laboratory capabilities, and investigating the emergence of new pathogens in tropical regions of South America.

BACKGROUND: Arthropod-borne viruses (arboviruses) are among the most common agents of human febrile illness worldwide and the most important emerging pathogens, causing multiple notable epidemics of human disease over recent decades. Despite the public health relevance, little is know about the geographic distribution, relative impact, and risk factors for arbovirus infection in many regions of the world. Our objectives were to describe the arboviruses associated with acute undifferentiated febrile illness in participating clinics in four countries in South America and to provide detailed epidemiological analysis of arbovirus infection in Iquitos, Peru, where more extensive monitoring was conducted. METHODOLOGY/FINDINGS: A clinic-based syndromic surveillance system was implemented in 13 locations in Ecuador, Peru, Bolivia, and Paraguay. Serum samples and demographic information were collected from febrile participants reporting to local health clinics or hospitals. Acute-phase sera were tested for viral infection by immunofluorescence assay or RT-PCR, while acute- and convalescent-phase sera were tested for pathogen-specific IgM by ELISA. Between May 2000 and December 2007, 20,880 participants were included in the study, with evidence for recent arbovirus infection detected for 6,793 (32.5%). Dengue viruses (Flavivirus) were the most common arbovirus infections, totaling 26.0% of febrile episodes, with DENV-3 as the most common serotype. Alphavirus (Venezuelan equine encephalitis virus [VEEV] and Mayaro virus [MAYV]) and Orthobunyavirus (Oropouche virus [OROV], Group C viruses, and Guaroa virus) infections were both observed in approximately 3% of febrile episodes. In Iquitos, risk factors for VEEV and MAYV infection included being male and reporting to a rural (vs urban) clinic. In contrast, OROV infection was similar between sexes and type of clinic. CONCLUSIONS/SIGNIFICANCE: Our data provide a better understanding of the geographic range of arboviruses in South America and highlight the diversity of pathogens in circulation. These arboviruses are currently significant causes of human illness in endemic regions but also have potential for further expansion. Our data provide a basis for analyzing changes in their ecology and epidemiology.

Relata-se o caso de uma paciente portadora de cardiomiopatia chagásica, internada no Hospital Beneficência Portuguesa, com insuficiência cardíaca congestiva classe funcional IV e arritmia ventricular complexa. Após avaliaçao, iniciou-se o protocolo para a realizaçao de transplante cardíaco. A paciente apresentou fibrilaçao ventricular, sendo recuperada por manobras de ressuscitaçao cárdio-pulmonar. Submetida a um estudo eletrofisiológico invasivo, desencadearam-se duas taquicardias ventriculares de origens distintas, mal toleradas hemodinamicamente, sendo indicado o implante de um cardioversor-desfibrilador. Reutilizou-se por 84 dias uma prótese com 3 meses de uso, explantada de outro paciente, até a realizaçao do transplante cardíaco, o que foi fundamental para garantir a sobrevida da paciente nesse período. Verificou-se que é possível reutilizar cardioversores-desfibriladores implantáveis uma vez que o alto custo da prótese, na época nao era coberto pelo SUS, o que impossibilitava o acesso da grande maioria dos pacientes a esse tipo de terapia. Justifica-se assim a adoçao dessa conduta, principalmente nos casos em que a perspectiva de uso do aparelho é por tempo limitado.

Relata-se o caso de uma paciente portadora de cardiomiopatia chagásica, internada no Hospital Beneficência Portuguesa, com insuficiência cardíaca congestiva classe funcional IV e arritmia ventricular complexa. Após avaliaçao, iniciou-se o protocolo para a realizaçao de transplante cardíaco. A paciente apresentou fibrilaçao ventricular, sendo recuperada por manobras de ressuscitaçao cárdio-pulmonar. Submetida a um estudo eletrofisiológico invasivo, desencadearam-se duas taquicardias ventriculares de origens distintas, mal toleradas hemodinamicamente, sendo indicado o implante de um cardioversor-desfibrilador. Reutilizou-se por 84 dias uma prótese com 3 meses de uso, explantada de outro paciente, até a realizaçao do transplante cardíaco, o que foi fundamental para garantir a sobrevida da paciente nesse período. Verificou-se que é possível reutilizar cardioversores-desfibriladores implantáveis uma vez que o alto custo da prótese, na época nao era coberto pelo SUS, o que impossibilitava o acesso da grande maioria dos pacientes a esse tipo de terapia. Justifica-se assim a adoçao dessa conduta, principalmente nos casos em que a perspectiva de uso do aparelho é por tempo limitado.

Relata-se o caso de uma paciente portadora de cardiomiopatia chagásica, internada no Hospital Beneficência Portuguesa, com insuficiência cardíaca congestiva classe funcional IV e arritmia ventricular complexa. Após avaliaçao, iniciou-se o protocolo para a realizaçao de transplante cardíaco. A paciente apresentou fibrilaçao ventricular, sendo recuperada por manobras de ressuscitaçao cárdio-pulmonar. Submetida a um estudo eletrofisiológico invasivo, desencadearam-se duas taquicardias ventriculares de origens distintas, mal toleradas hemodinamicamente, sendo indicado o implante de um cardioversor-desfibrilador. Reutilizou-se por 84 dias uma prótese com 3 meses de uso, explantada de outro paciente, até a realizaçao do transplante cardíaco, o que foi fundamental para garantir a sobrevida da paciente nesse período. Verificou-se que é possível reutilizar cardioversores-desfibriladores implantáveis uma vez que o alto custo da prótese, na época nao era coberto pelo SUS, o que impossibilitava o acesso da grande maioria dos pacientes a esse tipo de terapia. Justifica-se assim a adoçao dessa conduta, principalmente nos casos em que a perspectiva de uso do aparelho é por tempo limitado.

The management of the COVID-19 pandemic not only depends on the stringency measures established by governments but also and more importantly on the underlying capacity of territories in economic and health and sanitary infrastructure. This study aims to identify how the underlying conditions of countries influence on their level of COVID-19 lethality rate. To do so, a classification of countries is first conducted by the means of the k-means partitioning method, using COVID-19-related variables such as the lethality rate, the contagion growth rate and the number of days with respect to China. Based on the resulting groups of countries of the first stage, Tobit and Ordinary Least Squares regressions are estimated to determine the effect of the underlying characteristics of countries on their COVID-19 lethality rate. Risks factors which increase the lethality rate in countries are the contagion growth rate, the trade flow with China, the age composition of the population and, to a lesser extent, the population density. Factors that help to reduce the lethality rate are the government effectiveness, the health infrastructure (hospital beds) and, to a lesser extent, the economic growth rate.

This paper focuses on the relationship between imports, innovation and population agglomeration within regions in Colombia. The main aim is to identify the source of trade that shapes the relationship between innovation and agglomeration, by distinguishing imports ac-cording to levels of technological intensity. This study attempts to bridge two factors that have been studied separately: trade on the one hand; science, technology and innovation activity (STI), on the other. To control for unobserved heterogeneity, the Least Square Dummy Variable estimator with robust standard errors was employed. The results show that the technological intensity of imports has an impact on the agglomeration effect of STI activity. Moreover, the effect of STI activity on intra-regional agglomeration conditioned by imports varies according to regional characteristics

Radiosondes are the most widely used method for studies of vertical atmospheric behavior, but the high costs associated, and the logistic limitations have forced researchers to look for alternative methods for atmospheric profiling, such as lidar and satellite measurements, or modeling. However, the assessment of the accuracy of alternative methods is recommended, especially in complex terrain, such as the tropical Andes. In this research, the atmospheric profiling of satellite data from AIRS and MODIS products, simulations of the Weather Research and Forecasting model, WRF, and drone measurements are evaluated for a campaign of 10 radio soundings, between August 2021 and January 2022. Additionally, the capability to capture the planetary boundary layer height, hPBL, is studied. The measurements were conducted at Izobamba station near Quito, Ecuador. Temperature, T, Dew Point Temperature, TD, Mixing Ratio, Q, and Potential Temperature, PT, were evaluated from 0 to 300 m above ground level (magl.) for satellite, WRF, and drone data, and from 0 km to 15 km for satellite and WRF data. Additionally, the capability to capture the planetary boundary layer height, HPBL, was assessed. The results show that drone profiles best represented the magnitude of the analyzed variables showing mean RMSE of 0.79 for T, but the noise of the measurements caused a low correlation with radio sounding profiles, which was partially corrected with a quadratic fit on the profile. The WRF results achieved a positive representation in terms of correlation, but error metrics show that there are remarkable differences in magnitude in the first 300 magl., up to the tropopause height, which surpasses satellite representations for all variables. The MODIS profiles do not generally perform well due to their low vertical resolution and limitations with cloud coverage. However, AIRS data, despite its low resolution, show a better representation of vertical profiles than MODIS, for T and TD, surpassing WRF simulations in some dates. For the HPBL, the WRF results show that physical and atmospheric conditions limit its determination, and the methods and conditioning factors should be further analyzed.

This paper aims to determine the urban wage premium in Ecuador. It estimates two wage equations with the nominal and real wage, using instrumental variables to control endogeneity. Four indicators are applied to measure up the urban premium: area, classification by size of a city, cantonal population, and number of firms per capita. The latter is used for the first time and proves to be a better measure to reflect the urban premium. The results show that workers located in metropolis and big cities earn wages 14% and 4% higher than those workers located in small towns, respectively. The wage elasticity is 0.03% with respect to cantonal population, and 0.06% with respect to the number of firms.

We present findings of a project that investigated the potential of an online tandem program to enhance the foreign language learning of two groups of school-aged beginner learners, one learning English in Colombia and the other learning Spanish in New Zealand. We assessed the impact of the project on students’ learning with a free writing activity done as pretest and posttest and used a semi-structured interview to explore their attitudes towards language learning and their perceived development of their native language. Data analysis indicated statistically significant gains in foreign language writing and positive attitudinal changes toward foreign and native language learning.

Bacteria play a crucial role in the marine carbon cycle, contributing to the production and degradation of organic carbon. Here, we investigated organic carbon pools, aggregate formation, and bacterioplankton communities in three contrasting oceanographic settings in the Galapagos Archipelago. We studied a submarine CO2 vent at Roca Redonda (RoR), an upwelling site at Bolivar Channel (BoC) subjected to a weak El Niño event at the time of sampling in October 2014, as well as a site without volcanic or upwelling influence at Cowley Islet (CoI). We recorded physico-chemical parameters, and quantified particulate and dissolved organic carbon, transparent exopolymeric particles, and the potential of the water to form larger marine aggregates. Free-living and particle-attached bacterial communities were assessed via 16S rRNA gene sequencing. Both RoR and BoC exhibited temperatures elevated by 1–1.5 °C compared to CoI. RoR further experienced reduced pH between 6.8 and 7.4. We observed pronounced differences in organic carbon pools at each of the three sites, with highest dissolved organic carbon concentrations at BoC and RoR, and highest particulate organic carbon concentrations and aggregate formation at BoC. Bacterioplankton communities at BoC were dominated by opportunistic copiotrophic taxa, such as Alteromonas and Roseobacter, known to thrive in phytoplankton blooms, as opposed to oligotrophic taxa dominating at CoI, such as members of the SAR11 clade. Therefore, we propose that bacterial communities were mainly influenced by the availability of organic carbon at the investigated sites. Our study provides a comprehensive characterization of organic carbon pools and bacterioplankton communities, highlighting the high heterogeneity of various components of the marine carbon cycle around the Galapagos Archipelago.

El presente artículo pretende contribuir al análisis de un segmento de la élite judicial en Colombia. Con este objetivo, este artículo presenta el diseño institucional y las prácticas informales que acompañan los procesos de reclutamiento de los miembros de la Corte Suprema de Justicia de Colombia en un periodo histórico determinado. Además, muestra las principales características de las personas que han ocupado el cargo de Magistrado en el más Alto Tribunal de la justicia ordinaria. Finalmente, el artículo muestra una diversificación importante en el perfil socio-profesional de los miembros de la Corte Suprema de Justicia y la importancia creciente del capital social para ser elegido al cargo.

We present here the results for the kindergarten level of a qualitative research study on the impact of a curriculum connecting learningevents in Spanish and English in authentic communicative performances. The school in the study was a private institution in a mid-sizedColombian city. The curriculum is based on communicative and constructivist principles and presents the learning events as communicativeauthentic performances. We describe the performances that the kindergarten teachers developed based on the curriculum, how they achievedauthenticity in them, and their impact on the students’ attitude towards the English class and on their learning. We analyzed four teacherinterviews and four class observations done over a year, plus two more class recordings made independently by the teachers. They revealthat teachers used a variety of games, especially role-playing games developed from ideas from the children, reading of stories, and songsand that the students became enthusiastic and active and improved their oral skills considerably.

This paper presents the three main algorithms used for congestion control in communication networks: Leaky Bucket, Token Bucket and Virtual Scheduling. Its aim is to avoid traffic reaches unacceptable levels of congestion. It also offers some of the modifications made to these algorithms and their main applications.