1. Klebsiella pneumoniae Planktonic and Biofilm Reduction by Different Plant Extracts: In Vitro Study
- Author
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Lucas De Paula Ramos, Carlos Eduardo da Rocha Santos, Daphne Camargo Reis Mello, Lígia Nishiama Theodoro, Felipe Eduardo De Oliveira, Graziella N. Back Brito, Juliana Campos Junqueira, Antonio Olavo Cardoso Jorge, and Luciane Dias de Oliveira
- Subjects
Technology ,Medicine ,Science - Abstract
This study evaluated the action of Pfaffia paniculata K., Juglans regia L., and Rosmarius officinalis L. extracts against planktonic form and biofilm of Klebsiella pneumoniae (ATCC 4352). Minimum inhibitory concentration (MIC) and minimum microbicidal concentration (MMC) values were determined for each extract by microdilution broth method, according to Clinical and Laboratory Standards Institute. Next, antimicrobial activity of the extracts on biofilm was analyzed. For this, standardized suspension at 107 UFC/mL of K. pneumoniae was distributed into 96-well microplates (n=10) and after 48 h at 37°C and biofilm was subjected to treatment for 5 min with the extracts at a concentration of 200 mg/mL. ANOVA and Tukey tests (5%) were used to verify statistical significant reduction (p
- Published
- 2016
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