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Parkinson’s disease (PD) is the second leading neurodegenerative disease after Alzheimer’s disease. Mucuna pruriens (L.) DC. (MP) is a plant that contains Levodopa (L-DOPA) and has been known to improve the symptoms of PD. In this preliminary study, we investigated the anti-parkinsonian potential of MP to compare the effects of L-DOPA. We first developed an in vivo model of the PD in C57BL/6 male mice using rotenone. A total of twelve mice were used for this experiment. Nine mice were injected with rotenone (28 mg/kg) daily for 28 days. The mice experiments were performed to validate the effectiveness of MP to treat PD. Synthetic L-DOPA in a ratio of 1:20 with MP was used as MP contains 5% L-DOPA by weight in it. MP and L-DOPA were injected for 19 days on a daily basis. Cognitive function was evaluated using beam balance and olfactory tests. Serum analysis was performed using serum enzyme-linked immunosorbent assay (ELISA) analysis test. IL-12, IL-6, and TGF-β 1 were evaluated to validate the PD inducement and treatment. The levels of IL-12, IL-6, and TGF-β1 (p < 0.0001) in the PD mice group were significantly higher than those in the control group. The PD mice also showed higher latencies in beam balance and olfactory tests (p < 0.0001) compared to the control group. Both MP and L-DOPA-treated groups showed alleviation in latencies in beam balance and olfactory tests and decreased neuroinflammation in ELISA analysis (p < 0.001). The results treated by MP and L-DOPA showed insignificant differences in their values (p > 0.05). This proved that the MP and L-DOPA had similar effects in improving the symptoms of PD when used in the ratio of 1:20. Furthermore, both MP and L-DOPA reduced the level of IL-6 and TGF-β1 in this study. It may be inferred that a reduction in the level of IL-6 and TGF-β1 eventually leads to a reduction in the Th17 cells. The pathogenic Th17 is thought to be present in virtually all chronic inflammatory disorders. This can be an interesting area of research in further understanding the immunological effect of MP in ameliorating PD symptoms.

Abstract Nitrogen-tungsten co-doped titanium dioxide nanoparticles (W-N-doped TiO2 NPs) are employed for the photocatalytic degradation of environmental pollutants. However, the potential impact of these nanoparticles on the central nervous system remains a subject of concern. This study aimed to evaluate the effects of W-N-doped TiO2 NPs on neurophysiological and biochemical parameters of healthy rat brains, including behavioral monitoring, electroencephalogram analysis, and oxidative stress markers quantification. Intraperitoneal administration of W-N-doped TiO2 NPs to rats revealed abnormal brain electrical activity and an altered sense of balance in the treated rats. The ability of W-N-doped TiO2 NPs to cross the blood–brain barrier and accumulate in the brain leads to oxidative stress damage, supported by the elevated levels of reactive oxygen species (ROS), nitrite concentration, and malondialdehyde levels. Additionally, exposure to W-N-doped TiO2 NPs significantly reduced the antioxidant enzyme levels, such as catalase and superoxide dismutase, impacting a significant decrease in dopamine and acetylcholinesterase within the rat neural tissue. Furthermore, the inflammatory biomarker tumor necrosis factor-alpha and 8-hydroxy 2-deoxyguanosine significantly increased in response to W-N-doped TiO2 NPs. The findings revealed the adverse effects of W-N-doped TiO2 NPs on the electrical activity of rat brains and the altered concentration of various neuro-biomarkers, highlighting their potential neurotoxicity.

Background: The existing diagnostic methods for coronary artery disease (CAD), such as coronary angiography and fractional flow reserve (FFR), have limitations regarding their invasiveness, cost, and discomfort. We explored a novel diagnostic approach, coronary contrast intensity analysis (CCIA), and conducted a comparative analysis between it and FFR. Methods: We used an in vitro coronary-circulation-mimicking system with nine stenosis models representing various stenosis lengths (6, 18, and 30 mm) and degrees (30%, 50%, and 70%). The angiographic brightness values were analyzed for CCIA. The in vivo experiments included 15 patients with a normal sinus rhythm. Coronary angiography was performed, and arterial movement was tracked, enabling CCIA derivation. The CCIA values were compared with the FFR (n = 15) and instantaneous wave-free ratio (iFR; n = 11) measurements. Results: In vitro FFR showed a consistent trend related to the length and severity of stenosis. The CCIA was related to stenosis but had a weaker correlation with length, except for with 70% stenosis (6 mm: 0.82 ± 0.007, 0.68 ± 0.007, 0.61 ± 0.004; 18 mm: 0.78 ± 0.052, 0.69 ± 0.025, 0.44 ± 0.016; 30 mm: 0.80 ± 0.018, 0.64 ± 0.006, 0.40 ± 0.026 at 30%, 50%, and 70%, respectively). In vitro CCIA and FFR were significantly correlated (R = 0.9442, p < 0.01). The in vivo analysis revealed significant correlations between CCIA and FFR (R = 0.5775, p < 0.05) and the iFR (n = 11, R = 0.7578, p < 0.01). Conclusions: CCIA is a promising alternative for diagnosing stenosis in patients with CAD. The initial in vitro validation and in vivo confirmation in patients demonstrate the feasibility of applying CCIA during coronary angiography. Further clinical studies are warranted to fully evaluate the diagnostic accuracy and potential impact of CCIA on CAD management.

Neuromodulation is used to treat neurological disorders. Focused ultrasound can deliver acoustic energy to local regions of the brain, including deep brain structures. In addition, it is possible to induce the activation or inhibition of nerves through parameter adjustments of focused ultrasound. Laser-generated focused ultrasound (LGFUS) has demonstrated a potential use in precise therapeutic ultrasound applications owing to the ability to produce high-pressure, broadband frequency of shock waves with a tight focal spot, resulting in confined acoustic exposure of a small area. However, there have been few studies of neurostimulation using shock waves with pulse durations of several nanoseconds. The purpose of this study is to investigate the possibility of neurostimulation by shock waves generated from a focused Carbon Nanotube (fCNT) transducer. We measured electroencephalographic (EEG) signals in three rat brains before and after shock wave stimulation and compared them in the time and frequency domains. In the time domain, the number of peaks of EEG signals was measured significantly higher after shock wave stimulation than before stimulation in all three rats. The three rats showed differences in three frequency bands: theta(4-7 Hz), alpha(8-12), and 1–30 Hz, before and after shock wave stimulation (p < 0.001). These differences in EEG signals after shock wave stimulation of three rats were confirmed mainly because of shock waves. The stimulation of a rat brain was feasible using shock waves generated by the fCNT transducer. This study provides a basis for the applications of shock waves to brain stimulation for precise targeting.

Abstract Low-intensity ultrasound (LIUS) enhances the proliferation rate of various mammalian stem cells through mechanical stimulation. This study quantitively finds suitable LIUS stimulation parameters for increasing the proliferation rate of human adipose-derived mesenchymal stem cells (hAdMSCs) for mass production. Various stimulation conditions of LIUS were assessed based on the beam pattern of the ultrasonic transducer and the attenuation of the sound waves. Using optimal LIUS stimulation parameters for enhancing proliferation of hAdMSCs taken from bromodeoxyuridine (BrdU) incorporation assay, long-term culture of hAdMSCs was performed for 16 days. The resultant hAdMSCs were characterized for various biomarkers such as CD34−, CD45−, CD73+, CD95+, CD105+ and cytological staining and a cytokine array assay. LIUS stimulation parameters found for enhancing the hAdMSCs proliferation were the frequency of 5 MHz, an intensity of 300 mWcm−2, a duration of 10 min per day, and continuous waves with a 100% duty cycle. The LIUS stimulated hAdMSCs group showed a 3.25-fold increase in the cell number compared to the control group after 16 days of culture. By confirming the effects of quantitatively measured LIUS stimulation on the enhancement of hAdMSCs proliferation, this study may be a foundation for the applications of LIUS stimulation in the industrial-scale production of hAdMSCs.

The potential improvement in sonication efficiency achieved by tilting the focused ultrasound (FUS) transducer of the transcranial MR-guided FUS system is presented. A total of 56 cases of patient treatment data were used. The relative position of the clinical FUS transducer to the patient’s head was reconstructed, and region-specific skull density and porosity were calculated based on the patient’s CT volume image. The total transmission coefficient of acoustic waves emitted from each channel was calculated. Then, the total energy penetrating the human skull—which represents the sonication efficiency—was estimated. As a result, improved sonication efficiency was by titling the FUS transducer to a more appropriate angle achieved in all 56 treatment cases. This simulation result suggests the potential improvement in transcranial-focused ultrasound treatment by simply adjusting the transducer angle.

We are pleased to see that Marino et al. have written a Comment: “Choroidal Thickness Measurements in the Case of Diabetic Macular Edema” [...]

Abstract Previous studies on red blood cell (RBC) aggregation have elucidated the inverse relationship between shear rate and RBC aggregation under Poiseuille flow. However, the local parabolic rouleaux pattern in the arterial flow observed in ultrasonic imaging cannot be explained by shear rate alone. A quantitative approach is required to analyze the spatiotemporal variation in arterial pulsatile flow and the resulting RBC aggregation. In this work, a 2D RBC model was used to simulate RBC motion driven by interactional and hydrodynamic forces based on the depletion theory of the RBC mechanism. We focused on the interaction between the spatial distribution of shear rate and the dynamic motion of RBC aggregation under sinusoidal pulsatile flow. We introduced two components of shear rate, namely, the radial and axial shear rates, to understand the effect of sinusoidal pulsatile flow on RBC aggregation. The simulation results demonstrated that specific ranges of the axial shear rate and its ratio with radial shear rate strongly affected local RBC aggregation and parabolic rouleaux formation. These findings are important, as they indicate that the spatiotemporal variation in shear rate has a crucial role in the aggregate formation and local parabolic rouleaux under pulsatile flow.

Passive acoustic monitoring (PAM) is commonly utilized to monitor cetacean species’ distribution, abundance, and behavior. The demand for automated methods to detect and extract cetacean vocalizations from acoustic data has increased in the last few decades. Automatic whistle extraction of Indo-Pacific Bottlenose Dolphin (IPBD) and other whistle-producing delphinids habitating in the coastal areas represents a challenging problem due to the high ambient noise, including ship noise snapping shrimp at the same habitat. The acoustic signal containing snapping shrimp sound was usually excluded during the development of the detection method. A robust tool of bioacoustics for snapping shrimp sound is still lacking. This study trained a convolutional neural network (CNN) designed for semantic segmentation, initially developed for autonomous driving, to extract the whistle contour from spectrogram at a pixel level. A total of 1600 datasets was annotated for training and testing. As a result, the semantic segmentation classified the whistle with an overall mean Precision of 0.96, Accuracy of 0.89, and F-score of 0.86. In particular, the semantic segmentation extracted the whistle even if associated with a rich snapping shrimp sound, which the conventional method is incapable of. The advancement of metrics presented in this paper will enable long-term assessment of the IPBD population and individual or group tracking.

Abstract Background and objectives Fractional flow reserve (FFR) and instantaneous wave-free ratio (iFR) are the two most commonly used coronary indices of physiological stenosis severity based on pressure. To minimize the effect of wedge pressure (P wedge ), FFR is measured during hyperemia conditions, and iFR is calculated as the ratio of distal and aortic pressures (P d /P a ) in the wave-free period. The goal of this study was to predict P wedge using the backward wave (P back ) through wave separation analysis (WSA) and to reflect the effect of P wedge on FFR and iFR to identify the relationship between the two indices. Methods An in vitro circulation system was constructed to calculate P wedge . The measurements were performed in cases with stenosis percentages of 48, 71, and 88% and with hydrostatic pressures of 10 and 30 mmHg. Then, the correlation between P back by WSA and P wedge was calculated. In vivo coronary flow and pressure were simultaneously measured for 11 vessels in all patients. The FFR and iFR values were reconstructed as the ratios of forward wave at distal and proximal sites during hyperemia and at rest, respectively. Results Based on the in vitro results, the correlation between P back and P wedge was high (r = 0.990, p

In The above article [1], Fig. 5(b) is incorrectly presented as a duplicate of Fig. 5(a). The correct figure is presented here.

Abstract Background The phase correction on transcranial focused ultrasound is essential to regulate unwanted focal point shift caused by skull bone aberration. The aim of the current study was to design and investigate the feasibility of a ray-based phase correction toolkit for transcranial focused ultrasound. Results The peak pressure at focal area was improved by 140.5 ± 7.0% on target I and 134.8 ± 19.1% on target II using proposed phase correction toolkit, respectively. A total computation time of 402.1 ± 24.5 milliseconds was achieved for each sonication. Conclusion The designed ray-based phase correction software can be used as a lightweight toolkit to compensate aberrated phase within clinical environment.

Preservation of historical weapons requires continual and careful maintenance. Digital three-dimensional (3D) scanning can assist in preservation and analysis by generating a 3D computer model. New Zealand presents a special case for historical preservation, owing to the rapid import of European goods in a culture previously unexposed to metalworking. This, and the subsequent British colonization, led to upheaval and war. The most intense conflict between British and Maori forces was in the New Zealand Land Wars of the mid-19th century. The primary handheld firearms used in this period were black-powder muzzle-loaders, and the variety of armed factions involved in the war resulted in an eclectic range of weapons used. Two antique muzzle-loading pistols from this period were scanned and analyzed. Insights were gained into the history of double-barreled muzzle-loading pistols and transitional revolvers. The double-barreled pistol was determined to have been a flintlock pistol from a century prior to the Land Wars, later converted to percussion cap ignition. The transitional revolver was an intermediate step between the multi-barrel pepperbox pistol and the “true” revolver, but it remained in use throughout the Victorian era. Both types of firearms were effectively obsolete elsewhere in the world by the time of the Land Wars, but the conflict created a demand for a variety of weapons. While the pistols analyzed in this study are decommissioned and no longer in working order, the 3D models made from the samples afforded a unique glimpse into New Zealand’s history. The methodology detailed over the course of the study can be applied to other historical firearms in order to facilitate preservation, investigation, and experimentation. Highlights: • Preservation of historical machines requires continual maintenance, including replacement of worn or missing parts. • A combination of 3D scanning and digital models was used to analyze two antique pistols from New Zealand: a converted flintlock pistol and a transitional revolver. • The method of making and analyzing digital models detailed in this study offers a way to facilitate historical preservation, experimental archaeology, and functional analysis.

This observational study investigated the changes in choroidal thickness (ChT) in different patterns of diabetic macular edema (DME) based on image processing using enhanced-depth imaging spectral-domain optical coherence tomography (EDI-SD-OCT). Participants with ocular conditions affecting the fundus view, including retinal diseases, were excluded. After observing the patient’s medical record, multicolor fundus photos, thickness maps, and subtypes of DME were diagnosed according to the criteria reported by the Early Treatment Diabetic Retinopathy Study (ETDRS). Edema was classified as focal or diffuse and was subdivided into cystic macular edema (CME), CME with subretinal fluid (CME+), and spongy macular edema (SME). Image processing was performed on the B-scan images from SD-OCT to segment the choroid layer and obtain the choroid thickness. A total of 159 eyes of 81 patients (46 males and 35 females; 57.53 ± 9.78 years of age), and 57 eyes of 30 healthy individuals (age 57.34 ± 8.76 years) were enrolled in this study. Out of 159 eyes, 76 had focal macular edema (FME), 13 exhibited SME, and 51 presented CME. Among those with cystic macular edema, 19 eyes showed subretinal fluid (CME+). The average choroidal thickness in FME, diffuse SME, CME, and CME+ was 216.95 ± 52.94 µm, 243.00 ± 46.34 µm, 221.38 ± 60.78 µm, and 249.63 ± 53.90 µm, respectively. The average choroidal thickness in age-matched controls was 213.88 ± 45.60 µm. Choroidal thickness increases with the severity of edema; choroidal thickness was higher in diffuse macular edema than in FME. However, choroidal thickness increased in cystic macular edema with subretinal fluid (CME+).

Carbon nanotube–polymethyl siloxane (CNT-PDMS) composite transducers generate shock waves using optoacoustic technology. A thin layer of thermally conductive CNT and elastomeric polymer, PDMS, is applied on the concave surface of transparent polymethylmethacrylate (PMMA) to convert laser energy to acoustic energy using the thermoelastic effect of the composite transducer. The efficient conversion of laser energy requires an optimum utilization of the different properties of composite transducers. Among these properties, the diameter of composite transducers is a significant parameter. To practically verify and understand the effect of the diameter of composite transducers on the properties of shock waves, CNT-PDMS composite transducers with different diameters and focal lengths were constructed. Increases in the diameter of the composite transducer and input laser energy resulted in increased peak pressures of the shock waves. The maximum positive and negative pressures of the shock waves generated were 53 MPa and −25 MPa, respectively. This practically demonstrates that high peak amplitudes of shock waves can be achieved using larger transducers, which are suitable for practical applications in transcranial studies.

Numerous studies have evaluated the acoustic characteristics of soniferous snapping shrimp, but a few are based on long-term mooring measurements. In this study, underwater ambient noise signals were collected from a hydrophone moored 10 m from the sea bed in the coastal sea of western Jeju, South Korea, from mid-September 2019 for 90 days to analyze the variation in the sound of snapping shrimp. The kernel signal and a threshold value were utilized to identify the snapping shrimp, and the snap rate per minute was computed for quantitative analysis. The results show that the mean and standard deviation of the snap rate in the western sea of Jeju was 2132 ± 432 per minute during the whole measurement period. The surface water temperature and tidal level decreased by 7 °C from 25 °C and 50 cm from 190 cm, respectively, over 90 days. The snap rate decreased from September mainly due to the decrease in water temperature by 71 times per minute for every 1 °C decrease. It showed a circadian cycle, increasing by 17~24% at sunrise and sunset compared to the daytime minimum. The snap rate at night was the highest in late summer but the rate dropped like the one during the day in late fall. The snap rate at high tide was 13% higher on average than at low tide. The circadian and tidal changes of the snapping shrimp sound from long-term mooring measurements may be used as primary data for underwater ambient noise and the ecological behavior of snapping shrimp.

Drowsiness is a leading cause of traffic and industrial accidents, costing lives and productivity. Electroencephalography (EEG) signals can reflect awareness and attentiveness, and low-cost consumer EEG headsets are available on the market. The use of these devices as drowsiness detectors could increase the accessibility of safety and productivity-enhancing devices for small businesses and developing countries. We conducted a systemic review of currently available, low-cost, consumer EEG-based drowsiness detection systems. We sought to determine whether consumer EEG headsets could be reliably utilized as rudimentary drowsiness detection systems. We included documented cases describing successful drowsiness detection using consumer EEG-based devices, including the Neurosky MindWave, InteraXon Muse, Emotiv Epoc, Emotiv Insight, and OpenBCI. Of 46 relevant studies, ~27 reported an accuracy score. The lowest of these was the Neurosky Mindwave, with a minimum of 31%. The second lowest accuracy reported was 79.4% with an OpenBCI study. In many cases, algorithmic optimization remains necessary. Different methods for accuracy calculation, system calibration, and different definitions of drowsiness made direct comparisons problematic. However, even basic features, such as the power spectra of EEG bands, were able to consistently detect drowsiness. Each specific device has its own capabilities, tradeoffs, and limitations. Widely used spectral features can achieve successful drowsiness detection, even with low-cost consumer devices; however, reliability issues must still be addressed in an occupational context.

A non-invasive, brain-to-brain interface (BBI) requires precision neuromodulation and high temporal resolution as well as portability to increase accessibility. A BBI is a combination of the brain–computer interface (BCI) and the computer–brain interface (CBI). The optimization of BCI parameters has been extensively researched, but CBI has not. Parameters taken from the BCI and CBI literature were used to simulate a two-class medical monitoring BBI system under a wide range of conditions. BBI function was assessed using the information transfer rate (ITR), measured in bits per trial and bits per minute. The BBI ITR was a function of classifier accuracy, window update rate, system latency, stimulation failure rate (SFR), and timeout threshold. The BCI parameters, including window length, update rate, and classifier accuracy, were kept constant to investigate the effects of varying the CBI parameters, including system latency, SFR, and timeout threshold. Based on passively monitoring BCI parameters, a base ITR of 1 bit/trial was used. The optimal latency was found to be 100 ms or less, with a threshold no more than twice its value. With the optimal latency and timeout parameters, the system was able to maintain near-maximum efficiency, even with a 25% SFR. When the CBI and BCI parameters are compared, the CBI’s system latency and timeout threshold should be reflected in the BCI’s update rate. This would maximize the number of trials, even at a high SFR. These findings suggested that a higher number of trials per minute optimizes the ITR of a non-invasive BBI. The delays innate to each BCI protocol and CBI stimulation method must also be accounted for. The high latencies in each are the primary constraints of non-invasive BBI for the foreseeable future.

Acoustic waves are attenuated by fish schools as they propagate through the ocean. The attenuation by fish schools is not currently considered in fishery acoustics and sonar applications, especially at mid-frequency bands. In this study, fish school attenuation experiments were conducted with a number of individual fish in situ in a net cage at mid-frequency bands (3–7 kHz). The target fish species was the Japanese horse mackerel (Trachurus japonicus), which typically forms fish schools in the coastal ocean of northeastern Asia. The attenuated acoustic waves were measured for the cases of non-net, only net (0), 100, 200, 300, 400, and 500 individual horse mackerels in the net cage. Results showed that the acoustic signal attenuation increased with the number of horse mackerels. The mean and maximum attenuation coefficients were approximately 6.0–15.4 dB/m and 6.5–21.8 dB/m for all frequencies, respectively. The measured attenuation coefficients were compared with the ones from previous studies to propose new regression models with normalized extinction cross-sections of weight and length of fish. This study confirmed that the fish school attenuation could not be ignored and compensated at mid-frequencies in the ocean. These results would be useful for fishery acoustics, especially in the development of scientific echo-sounder, and naval applications of sonar operations and analysis.

The mechanical scanning of a single element transducer has been mostly utilized for high-frequency ultrasound imaging. However, it requires space for the mechanical motion of the transducer. In this paper, a rotational scanning ultrasound biomicroscopy (UBM) system equipped with a high-frequency angled needle transducer is designed and implemented in order to minimize the space required. It was applied to ex vivo ultrasound imaging of porcine posterior ocular tissues through a minimal incision hole of 1 mm in diameter. The retina and sclera for the one eye were visualized in the relative rotating angle range of 270° ~ 330° and at a distance range of 6 ~ 7 mm, whereas the tissues of the other eye were observed in relative angle range of 160° ~ 220° and at a distance range of 7.5 ~ 9 mm. The layer between retina and sclera seemed to be bent because the distance between the transducer tip and the layer was varied while the transducer was rotated. Certin features of the rotation system such as the optimal scanning angle, step angle and data length need to be improved for ensure higher accuracy and precision. Moreover, the focal length should be considered for the image quality. This implementation represents the first report of a rotational scanning UBM system.

Despite considerable research efforts on the relationship between arterial geometry and cardiovascular pathology, information is lacking on the pulsatile geometrical variation caused by arterial distensibility and cardiomotility because of the lack of suitable in vivo experimental models and the methodological difficulties in examining the arterial dynamics. We aimed to investigate the feasibility of using a chick embryo system as an experimental model for basic research on the pulsatile variation of arterial geometry. Optical microscope video images of various arterial shapes in chick chorioallantoic circulation were recorded from different locations and different embryo samples. The high optical transparency of the chorioallantoic membrane (CAM) allowed clear observation of tiny vessels and their movements. Systolic and diastolic changes in arterial geometry were visualized by detecting the wall boundaries from binary images. Several to hundreds of microns of wall displacement variations were recognized during a pulsatile cycle. The spatial maps of the wall motion harmonics and magnitude ratio of harmonic components were obtained by analyzing the temporal brightness variation at each pixel in sequential grayscale images using spectral analysis techniques. The local variations in the spectral characteristics of the arterial wall motion were reflected well in the analysis results. In addition, mapping the phase angle of the fundamental frequency identified the regional variations in the wall motion directivity and phase shift. Regional variations in wall motion phase angle and fundamental-to-second harmonic ratio were remarkable near the bifurcation area. In summary, wall motion in various arterial geometry including straight, curved and bifurcated shapes was well observed in the CAM artery model, and their local and cyclic variations could be characterized by Fourier and wavelet transforms of the acquired video images. The CAM artery model with the spectral analysis method is a useful in vivo experimental model for studying pulsatile variation in arterial geometry.