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IntroductionAcinetobacter baumannii (AB) is rising as a human pathogen of critical priority worldwide as it is the leading cause of opportunistic infections in healthcare settings and carbapenem-resistant AB is listed as a “super bacterium” or “priority pathogen for drug resistance” by the World Health Organization.MethodsClinical isolates of A. baumannii were collected and tested for antimicrobial susceptibility. Among them, carbapenem-resistant and carbapenem-sensitive A. baumannii were subjected to prokaryotic transcriptome sequencing. The change of sRNA and mRNA expression was analyzed by bioinformatics and validated by quantitative reverse transcription-PCR.ResultsA total of 687 clinical isolates were collected, of which 336 strains of A. baumannii were resistant to carbapenem. Five hundred and six differentially expressed genes and nineteen differentially expressed sRNA candidates were discovered through transcriptomic profile analysis between carbapenem-resistant isolates and carbapenem-sensitive isolates. Possible binding sites were predicted through software for sRNA21 and adeK, sRNA27 and pgaC, sRNA29 and adeB, sRNA36 and katG, indicating a possible targeting relationship. A negative correlation was shown between sRNA21 and adeK (r = -0.581, P = 0.007), sRNA27 and pgaC (r = -0.612, P = 0.004), sRNA29 and adeB (r = -0.516, P = 0.020).DiscussionThis study preliminarily screened differentially expressed mRNA and sRNA in carbapenem-resistant A. baumannii, and explored possible targeting relationships, which will help further reveal the resistance mechanism and provide a theoretical basis for the development of drugs targeting sRNA for the prevention and treatment of carbapenem-resistant A. baumannii infection.

Although straw returning to the field (SRTTF) is conducive to promoting sustainable agricultural production and protecting the environment, straw resources are still wasted due to the lack of suitable straw-returning technology in southern China. Based on the statistical yearbook and a large number of studies, different methods were used to calculate the total straw resources and SRTTF potential, and differences in these methods were compared. The results indicate that the total amount of straw resources in southern China in 2021 was 3.35×108 t. The nutrient content of K2O in the straw accounted for the highest proportion of total nutrient resources (63.66%), followed by N (26.88%) and P2O5 (9.46%). In theory, total SRTTF could replace almost all K2O and part of N and P2O5, indicating that the nutrient substitution potential of SRTTF was high. It is suggested that the SRTTF method be adopted in the middle and lower reaches of the Yangtze River, which mainly uses direct returning (DR) supplemented by indirect returning (IDR). In southeast China, straw returning is carried out by the combination of IDR and IR. In southwest China, straw returning is mainly carried out by IR and supplemented by MDR. This study will provide theoretical support for the government to formulate straw-returning policy.

BackgroundCurrent studies indicate a contradictory relationship between decreased mortality risks of spontaneous intracerebral hemorrhage (sICH) and elevated low-density lipoprotein cholesterol (LDL-C) levels. Thus, this meta-analysis was designed to examine the involvement of high LDL-C levels in a lower mortality risk of sICH patients.MethodsPubMed, Cochrane, and Embase databases were searched up to the date of August 3rd, 2022. Pooled odds ratio (OR) with a 95% confidence interval (CI) was estimated for the higher vs. lower serum LDL-C level groups. Subgroup and sensitivity analyses were also carried out. Egger's test was applied to detect any potential publication bias.ResultsOf 629 citations reviewed, 8 eligible cohort studies involving 83,013 patients were enrolled in this meta-analysis. Compared with lower serum LDL-C levels containing patients, higher serum LDL-C patients exhibited significantly decreased risks of 3-month mortality (OR: 0.51; 95%CI: 0.33–0.78; I2 = 47.8%); however, the LDL-C level change wasn't significantly associated with in-hospital mortality risks (OR: 0.92; 95%CI: 0.63–1.33; I2 = 91.4%) among sICH subjects. All studies included were classified as high-quality investigations.ConclusionsThis meta-analysis suggests a higher LDL-C level may decrease the mortality risk in sICH patients. LDL-C level increase is inversely associated with the 3-month mortality risks in these patients but not significantly correlated with the in-hospital mortality risks. Further well-designed prospective studies with extended follow-up periods are needed to confirm these findings and explore underlying cross-talks.Systematic review registrationhttps://www.crd.york.ac.uk/prospero/display_record.php?ID=CRD42022318318, identifier: PROSPERO 2022 CRD42022318318.

Abies fanjingshanensis trees are the only remaining Abies species in a type of subtropical forest of southwest China and are in imminent danger. Previous studies suggested that the massive death of Abies was caused by the unbalanced chemometrics and nutrients in the soil. To the best of our knowledge, for the first time, we evaluated the microbial carbon use efficiency (CUE) in the rhizospheric topsoil and subsoil of A. fanjingshanensis, at high elevation, middle elevation, and low elevation as well as investigated their physicochemical indices, soil enzyme activities, bacteria, fungi, and microbial biomass. The results showed that the physicochemical parameters (TP, SOC, AK, AP, MC, TN, NO3-N, NH4-N and cation exchange capacity) of the topsoil were higher than those of the subsoil. Acidobacteria, Proteobacteria, Planctomycetes, and Actinobacteria were the dominant phyla in the two soil layers. Candidatus_Koribacter was the main indicator species in the rhizospheric topsoil and subsoil. The positive correlation in the bacterial co-occurrence networks implied that cooperation was dominant between the bacteria in four soil types, and the same phenomenon was found in the co-occurrence networks of fungi. A structural equation model confirmed that pH was the most important factor affecting microbial CUE in the topsoil and subsoil. We inferred that the microorganisms in the acidic soil environment were forced to consume more energy to maintain cellular pH, while less energy was used for growth. The increased solubility of some toxic metals in the acidic soil affected the microbes, resulting in a lower microbial CUE in the A. fanjingshanensis rhizospheric soil. Our results highlight that pH values in soil mainly affected microbial CUE, and a lower microbial CUE may be another important factor in the death of large numbers of A. fanjingshanensis. Several measures must be carried out to improve the microbial CUE in the rhizospheric soil of A. fanjingshanensis by the department of forest management, such as adding the appropriate biochar and nitrogenous fertilizer.

Abstract Background Aedes (Stegomyia) albopictus (Skuse, 1894) is the main vector of dengue virus in China. The resistance to insecticides is a huge obstacle for the control of this species, and determining its resistance status and mechanisms in China is essential for the implementation of vector management strategies. Methods We have investigated the larval and adult resistance status of Ae. albopictus to deltamethrin in eight field populations in China. Mutations at the voltage-gated sodium channel gene, related to the knockdown resistance (kdr) effect, were detected by sequencing of PCR products. The eight field populations were examined for pyrethroid resistance using the World Health Organization standard bioassays, and the association between the mutations and phenotypic resistance was tested. Results The eight field populations of larvae of Ae. albopictus in China exhibited high resistance to deltamethrin; the RR50 values ranged from 12 (ZJ) to 44 (GZ). Adult bioassay revealed that Ae. albopictus populations were resistant to deltamethrin (mortality rate

This study investigated the relationship between the characteristics of quality components and trace elements of Niaowang tea from Guizhou Province in mountainous plateau areas. The contents of catechin monomers and eight other trace elements were measured using high-performance liquid chromatography (HPLC) and inductively coupled plasma mass spectrometry (ICP-MS), respectively. The results showed that the tender summer leaves of Niaowang tea in Guizhou Province had the highest content of catechins at 3558.15~2226.52 μg·g−1. The content of ester catechins was the highest in summer, amounting to 69.75~72.42% of the total catechins. The content of non-ester catechins was the highest in autumn, reaching 52.54~62.28% of the total catechins; among ester catechins, the mass fraction of epigallocatechin gallate (EGCG) showed a pattern of mature summer leaves > tender summer leaves > mature autumn leaves > tender autumn leaves, and the mass fractions of gallocatechin gallate (GCG) and epicatechin gallate (ECG) were larger in autumn than in summer; gallocatechin (GC) had no significant correlation with different trace elements, and Mn had no significant correlations with different catechin monomers. EGCG was significantly negatively correlated with As, Se, Hg, Pb, Ni, and Zn. Additionally, gallic acid (GA) was significantly negatively correlated with As, Hg and Ni. Other catechin monomers were largely significantly positively correlated with trace elements. The biochemical indicators of the phenotype of Niaowang tea show that the summer and autumn buds are suited for making high-quality green tea.

The Wanshan mercury (Hg) mine in Guizhou Province is one of the main Hg-producing mines in China, resulting in serious Hg pollution in soil and wastewater. Therefore, the present study is mainly aimed to investigate the current degree of heavy metal pollution and compared the microbial diversity in the Wanshan Hg mine and its surrounding environment. The results showed the distribution of the pollution load index values was low in the west and high in the east. The northwestern (Aozhai River), northern (Meizi Stream), and southwestern parts of the study area and the area surrounding Erkeng did not reach moderate pollution. Mercury accounted for the majority of the potential ecological risk index values, reaching 67.62%, while the proportions of Cd and As were 15.75% and 10.75%, respectively. Mercury was found mainly in a residual state, which had an average proportion of 71.09%. In the three regions, Proteobacteria and Actinobacteria had the highest relative abundances. According to linear discriminant analysis effect size, the indicator species in the Hg mining area, woodland and cultivated land was f__67–14 (belonging to a family of Solirubrobacterales), Reyranellales and Reyranellaceae, Intrasporangiaceae, respectively. In summary, this study for the very first time estimated that the higher Hg, Cd and As pollution existed in Wanshan Hg mine since their concentration in the all soil samples totally exceeded the standard value (GB15618-2018), while Cd and As pollution in soil was commonly ignored by the previous study. The cultivated land had higher community richness than the mercury mining district and woodland. Our results suggested that the relevant local departments need to take more active measures to solve the problem of high levels of Hg, Cd, and As in the local soil, and prevent their adverse effects on humans.

Davidia involucrata Baill (in Nyssaceae family) is a relic species of tertiary paleo tropical relic in China. It belongs to class I of the national key protected endangered plant. Recently, there are signs of large-scale death. This study investigated the population of Davidia involucrata Baill in the Mount Fanjing Nature Reserve and used field sampling and laboratory analysis to systematically study the stoichiometric characteristics of C, N and P in the plants and the soil and their driving factors. The results indicate that the average contents of organic carbon, total nitrogen, total phosphorus, hydrolysable nitrogen and available phosphorous in soils from Davidia involucrata Baill lands were 24.06 g kg−1, 2.22 g kg−1, 4.94 g kg−1, 168.84 mg kg−1 and 58.30 mg kg−1, respectively. The total nitrogen, sodium and zinc contents and the physical and chemical soil characteristics were positively correlated with the C, N and P contents in the leaves of Davidia involucrata Baill. The available phosphorous, magnesium, total phosphorous and available potassium in the soil were positively correlated with the C and N contents in the stems of Davidia involucrata Baill. The growth of Davidia involucrata Baill is substantially limited by organic C. We hope this study could provide some theoretical basis for scientific and effective protection of Davidia involucrata in Fanjing Mountain.

To study the spatial distribution characteristics of soil organic carbon (SOC) coupled with rocky desertification, 1212 soil samples from 152 soil profiles were sampled from different karst landforms, including karst low hills/virgin forest (KLH) in Libo County, a karst peak-cluster depression (KPCD) in Xingyi County, a karst canyon (KC) in Guanling County, a karst plateau basin (KPB) in Puding County and a karst trough valley (KTV) in Yinjiang County. The spatial distribution characteristics of the responses of SOC, SOC density (SOCD), rocky desertification and soil bulk density (SBD) to different influencing factors were analyzed. The relationships among SOC, SOCD, rocky desertification and SBD were analyzed using Pearson correlation analysis. The SOC storage capacity was characterized by using SOCD, and then the SOC storage capacity in different evolution stages of karst landforms was assessed. The SOC contents of KLH, KPCD, KC, KPB and KTV ranged from 6.16 to 38.20 g·kg−1, 7.42 to 27.08 g·kg−1, 6.28 to 35.17 g·kg−1, 4.62 to 23.79 g·kg−1 and 5.24 to 37.85 g·kg−1, respectively, and their average SOCD values (0–100 cm) were 7.37, 10.79, 7.06, 8.51 and 7.84 kg·m−2, respectively. The karst landforms as ordered by SOC storage capacity were KPCD > KPB > KLH > KTV > KC. The SOC content was negatively correlated with the SBD; light rocky desertification may lead to SOC accumulation. The rocky desertification degree and SBD were closely associated with slope position and gradient. Rocky desertification first increased, then decreased from mountain foot to summit, and increased with increasing slope gradient. However, the SBD decreased from mountain foot to summit and with increasing slope gradient. The SOC contents on the northern aspect of the mountains were generally higher than the other aspects. In summary, rock outcrops controlled the SOC contents in the studied regions. The slope position, gradient and aspect influenced the composition and distribution of vegetation, which influenced the evolution of rocky desertification. Therefore, these factors indirectly affected the SOC content. Additionally, the SOCD decreased with increasing rocky desertification. During the different evolution stages of karst landforms, the SOC storage capacity first decreases, then increases.

Abstract Background Akkermansia muciniphila is one of the most dominant bacteria that resides on the mucus layer of intestinal tract and plays key role in human health, however, little is known about its genomic content. Results Herein, we for the first time characterized the genomic architecture of A. muciniphila based on whole-genome sequencing, assembling, and annotating of 39 isolates derived from human and mouse feces. We revealed a flexible open pangenome of A. muciniphila currently consisting of 5644 unique proteins. Phylogenetic analysis identified three species-level A. muciniphila phylogroups exhibiting distinct metabolic and functional features. Based on the comprehensive genome catalogue, we reconstructed 106 newly A. muciniphila metagenome assembled genomes (MAGs) from available metagenomic datasets of human, mouse and pig gut microbiomes, revealing a transcontinental distribution of A. muciniphila phylogroups across mammalian gut microbiotas. Accurate quantitative analysis of A. muciniphila phylogroups in human subjects further demonstrated its strong correlation with body mass index and anti-diabetic drug usage. Furthermore, we found that, during their mammalian gut evolution history, A. muciniphila acquired extra genes, especially antibiotic resistance genes, from symbiotic microbes via recent lateral gene transfer. Conclusions The genome repertoire of A. muciniphila provided insights into population structure, evolutionary and functional specificity of this significant bacterium.