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R Olivera,1 LN Moro,2 R Jordan,1 N Pallarols,3 A Guglielminetti,3 C Luzzani,2 SG Miriuka,2 G Vichera1 1KHEIRON S.A Laboratory, Pilar, Buenos Aires, Argentina; 2LIAN-Unit Associated with CONICET, FLENI, Belen de Escobar, Buenos Aires, Argentina; 3Kawell Equine Hospital, Solís, Buenos Aires, Argentina Introduction: Cell plasticity is crucial in cloning to allow an efficient nuclear reprogramming and healthy offspring. Hence, cells with high plasticity, such as multipotent mesenchymal stem cells (MSCs), may be a promising alternative for horse cloning. In this study, we evaluated the use of bone marrow-MSCs (BM-MSCs) as nuclear donors in horse cloning, and we compared the in vitro and in vivo embryo development with respect to fibroblasts. Materials and methods: Zona-free nuclear transfer was performed using BM-MSCs (MSC group, n=3432) or adult fibroblasts (AF group, n=4527). Embryos produced by artificial insemination (AI) recovered by uterine flushing and transferred to recipient mares were used as controls (AI group). Results: Blastocyst development was higher in the MSC group than in the AF group (18.1% vs 10.9%, respectively; p