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The article explores the transnational dimension of adhocratic governance through the case study of the Turkmen community in Lebanon, a marginal and seemingly insignificant group, especially from a demographic point of view. It investigates how Turkey’s and Lebanon’s governance of Turkmen communities has evolved over the past few decades and what this reveals about how the Turkmen’s transnational identities operate in practice. Drawing on extensive fieldwork conducted by the authors in both Lebanon and Turkey, the paper contends that in recent years, Turkey has implemented ad hoc strategies towards Turkmen communities, influencing their transnational identification practices. While Lebanon has disengaged from the management of a population living in marginal yet strategically relevant areas, Turkey has framed its foreign policy in terms of cultural-religious kinship and pragmatism. Both political processes are continually characterised by competing bundles of identifications, which temporarily and incompletely acquire a privileged status in (especially foreign policy) decision-making. Keywords: Lebanon, Turkey, Transnational Identities, Adhocracy, Transnational Governance, Kinship

The spread of identity politicisation has revitalised the debate over the nature and transformations of the State and its role in the international arena. This shift has also underscored the importance of identity assumed in international relations (IR) as it offers an alternative to the traditional realist-rationalist vocabulary. Grounded on a counter-substantialist approach to identity the Special Issue moves the gaze from identity to identification processes. Identity as an essential object that explains actions fails to capture the constitutive relationship between actors’ identities and the social contexts in which they emerge. Consequently, the notion of individual or state identity as a foundation for actions is replaced by a process involving multiple acts of identification that occur in specific contexts and periods. How do identities, both as “identities in practice” and as given and abstract objects, influence states’ domestic and foreign governance? To answer this overarching question, the Special Issue draws on two interrelated notions of identity: as bundles of actions of identification that acquire temporarily privileged status in specific discourses and as a causal variable in IR that explains why states act in certain ways. This dual analytical lens allows to examine (1) the centrality of space and time in debates on how identity has contributed to redefining the role of the State domestically and its relationships in the international arena and (2) the extent and ways in which ad hoc measures at the micro-scale of identification processes influence the macro-level identity–legitimacy nexus. This framework aligns identity studies with the concept of “adhocracy”, defined in bureaucracy and policy studies as either an intentional institutional setup or an unintentional outcome of policy implementation. Keywords: State; Adhocratic Governance; Identity; Identification Processes; Space; Time

Cranial cruciate ligament (CrCL) disease is a common orthopedic disease in canine patients. Tibial osteotomy procedures for the treatment of cranial cruciate ligament disease in small breed dogs (6–12 months) post-operatively, 92.3% had no lameness, with the remaining 7.7% having Grade 1 lameness. A good to excellent clinical outcome was noted in all 26 stifles that underwent TTA with novel mini plates.

In previous works, we reported that Solid Oxide Fuel Cells (SOFCs), having a room-temperature sputtered and then annealed GDC thin film as the cathode/electrolyte barrier layer, had showed a huge increase of the output current (up to +78%) and a decrease of the ohmic resistance (up to -42%) as compared to fully screen-printed industrial SOFCs. We correlated the performance improvement to grain size in the GDC layer as a function of annealing temperature. However, no information on the density and activity of oxygen vacancies in the thin film to correlate with functionality could be extrapolated in these studies. Element and valence sensitive probes such as X-Ray Photoelectron Spectroscopy (XPS) and X-Ray Absorption Spectroscopy (XAS) enable atomic level characterization of nanostructured granular GDC layers deposited on polycrystalline anode/electrolyte bilayer substrates and the interplay between morphology and stoichiometry in determining the Ce3+/Ce4+ ratio, which in turns regulates their ionic and electronic conductivity. Here we show the results obtained on three room-temperature RF-sputtered GDC thin films, annealed with the same annealing ramp but at different plateaux temperatures, making use of XPS measurements to study the unreacted surface and the operando XAS to monitor the changes in Ce3+/Ce4+ ratio in different reactive atmospheres (i.e. neutral, oxidizing and reducing). The latter were carried out using the ambient pressure cell available at APE-HE beamline (Elettra synchrotron in Trieste, Italy). Our measurements allowed to determine the role of the annealing parameters in the number of available oxygen vacancies available in the oxygen reduction reaction (ORR), highlighting the different modifications induced in the investigated samples by the annealing process.

This article investigates how the fight against Islamophobia both clarifies and shapes the contours of Turkey's diaspora policy. It relies on the literature on political remittances and its value to diaspora studies by highlighting to what extent and how the commitment to tackling Islamophobia plays a role in Turkey's attempt to strengthen the link with 'its' diaspora. In this process, attention is devoted to how Turkish state institutions like the Presidency of Religious Affairs (Diyanet) have framed Islamophobia as a religion-based problem and an "anti-Islamic project". In so doing, they bolster a dichotomic narrative between secular Europe and Muslim migrants. The empirical discussion also reveals the international dimension of the fight against Islamophobia and examines the Turkish government's motivation to proclaim itself the defender of (Turkish) Muslim communities in Europe. Thus, the official narrative that overlooks any systemic discriminations Turkish minorities are experiencing in everyday life has promoted a tutelary representation that might reinforce a paternalistic view of the diasporas as victims who need saving.

IntroductionAccurate radiographic assessment of bone healing is vital in determining both clinical treatment and for assessing interventions aimed at the promotion of bone healing. Several scoring systems have been used to evaluate osteotomy changes following tibial plateau leveling osteotomy (TPLO). The goal of this study was to compare the ability of five radiographic scoring systems to identify changes in bone healing following TPLO over time (Aim I), and to evaluate the influence of limb positioning on TPLO osteotomy scoring (Aim II).Materials and methodsPhase I-A randomized, blinded, prospective study was conducted using similarly positioned postoperative TPLO radiographs from seven dogs taken immediately postoperatively, 6-weeks, and 8-weeks postoperatively. Ten reviewers assessed the radiographs, and five different scoring systems were tested for each set including three previously published ones, a Visual Analog Score (VAS), and a subjective 11-point scale. For each system, responses for 6-week postoperative were compared to 8-week postoperative. Scores were judged as correct (=showing an increase in score), incorrect (=decrease in score), or unchanged (=same score). Phase II-An international group of 39 reviewers was asked to score radiographs from three dogs, taken in different positions, using the VAS grading system. Scores were averaged and comparisons were made for each set.ResultsPhase I-The VAS system identified the greatest number of sets correctly (76%), with the least unchanged scores (15%), and 9% incorrect scores. Phase II-All three patients had an increase in the average difference between VAS-scores for differently positioned radiographs compared to similarly positioned radiographs. The magnitude of change between different positions far exceeded the magnitude of comparison of the similarly positioned radiographs from the 6- and 8-week time point.Discussion/ConclusionThe VAS system appears to be the most appropriate of the tested systems to identify small changes in bone healing. In addition, the positioning of postoperative TPLO radiographs makes a substantial difference in the healing score that is assigned. Care must be undertaken when performing postoperative radiographs in both the clinical and research setting to ensure accurate assessment of bone healing.

Since the Covid-19 emergency broke out, Turkish state institutions became crucial to govern diaspora communities facing lockdown measures and forced separation from the homeland. Being the first European country strongly affected by COVID-19 and where massive lockdown measures have been put in place, Italy is a relevant case to analyze. Retracing the scope and scale of the online activities organized during the COVID-19 pandemic, this paper investigates how the Turkish state supported Turkey-originated population living in Italy. The analysis draws on interviews with Diyanet religious officers sent from Ankara to serve the Diyanet’s branches (DITIB) in different Italian cities and with the religious attaché employed in the Turkish Consulate in Milan who supervises them. The interviews have been corroborated with the collection of a vast visual material including brochures, videos and posters published on YouTube channel and the Facebook official pages of the DITIB cultural centers in Italy. Our data show that, during COVID-19 pandemic, online seminars enabled to strengthen Turkey's connection with the diaspora using the emergency situation as a starting point for enhancing family programs and fostering a Muslim-Turkish belonging in any aspect of life.

Since the Covid-19 emergency broke out, Turkish state institutions became crucial to govern diaspora communities facing lockdown measures and forced separation from the homeland. Being the first European country strongly affected by COVID-19 and where massive lockdown measures have been put in place, Italy is a relevant case to analyze. Retracing the scope and scale of the online activities organized during the COVID-19 pandemic, this paper investigates how the Turkish state supported Turkey-originated population living in Italy. The analysis draws on interviews with Diyanet religious officers sent from Ankara to serve the Diyanet’s branches (DITIB) in different Italian cities and with the religious attaché employed in the Turkish Consulate in Milan who supervises them. The interviews have been corroborated with the collection of a vast visual material including brochures, videos and posters published on YouTube channel and the Facebook official pages of the DITIB cultural centers in Italy. Our data show that, during COVID-19 pandemic, online seminars enabled to strengthen Turkey's connection with the diaspora using the emergency situation as a starting point for enhancing family programs and fostering a Muslim-Turkish belonging in any aspect of life.

Since the Covid-19 emergency broke out, Turkish state institutions became crucial to govern diaspora communities facing lockdown measures and forced separation from the homeland. Being the first European country strongly affected by COVID-19 and where massive lockdown measures have been put in place, Italy is a relevant case to analyze. Retracing the scope and scale of the online activities organized during the COVID-19 pandemic, this paper investigates how the Turkish state supported Turkey-originated population living in Italy. The analysis draws on interviews with Diyanet religious officers sent from Ankara to serve the Diyanet’s branches (DITIB) in different Italian cities and with the religious attaché employed in the Turkish Consulate in Milan who supervises them. The interviews have been corroborated with the collection of a vast visual material including brochures, videos and posters published on YouTube channel and the Facebook official pages of the DITIB cultural centers in Italy. Our data show that, during COVID-19 pandemic, online seminars enabled to strengthen Turkey's connection with the diaspora using the emergency situation as a starting point for enhancing family programs and fostering a Muslim-Turkish belonging in any aspect of life.

We investigated the influence of a fuel change from pure hydrogen to a hydrogen–ammonia mixture at different percentages on the electrochemical behavior of 50 mm in diameter Solid Oxide Fuel Cells (SOFCs) with sputtered thin buffer layers of Gd-doped ceria, varying the working temperatures from 800 °C to 650 °C. The results show that the performances of the cells are not affected by the fuel change for high working temperatures (800 °C and 750 °C). As an example, a power density value of 802 mW∙cm−2 at 1 A∙cm−2 is found when directly feeding the cell with 8 NmL∙min−1cm−2 of ammonia and with an equivalent flowrate of 12 NmL∙min−1cm−2 of H2. These power density output values are higher than those obtained in industrial state-of-art (SoA) SOFCs with screen-printed buffer layers fed with equivalent hydrogen flowrates, thanks to the improved electrochemical performances obtained in the case of cells with sputtered thin buffer layers of Gd-doped ceria. At lower working temperatures (700 °C and 650 °C), slight changes in the electrochemical behavior of the cells are observed. Nevertheless, in this temperature range, we also obtain an output current density value of 0.54 A∙cm−2 in a pure ammonia flowrate of 12 NmL min−1cm−2 at 800 mV and 700 °C, equal to the value observed in SoA button cells with industrial screen-printed GDC barrier layer fueled with 16 NmL∙min−1cm−2 of H2. These results pave the way towards the use of innovative SOFC structures with sputtered thin buffer layers fueled by ammonia.

The purpose of this study was to report approaches to surgical and medical management of proximal tibial metaphyseal fractures (PTMF) and short-term case outcome. Medical records of immature dogs with PTMF were reviewed and data were collected including history, signalment and side affected. Data pertaining to surgical and medical management including radiographic evaluation and short-term complications were recorded. Forty-five dogs with a total of 47 PTMF identified and treated between 2007–2019 were included in this study. Six cases were managed with external coaptation alone. Forty-one cases were treated surgically with constructs including K-wires in different configurations, bone plate and screws, and external skeletal fixation. Of the cases managed conservatively, 4 developed complications, including bandage sores, diffuse osteopenia of the tarsus/metatarsus, and angular limb deformities. Surgical complications including pin migration necessitating removal, osteopenia, and screw placement in the proximal tibial growth plate or into the stifle joint were found in 16 cases. PTMF treated with surgery had a subjectively more predictable outcome compared to those treated with external coaptation alone. Conservative management may result in complications including development of excessive tibial plateau angle (TPA) as well as distal tibial valgus.

The purpose of this study was to report approaches to surgical and medical management of proximal tibial metaphyseal fractures (PTMF) and short-term case outcome. Medical records of immature dogs with PTMF were reviewed and data were collected including history, signalment and side affected. Data pertaining to surgical and medical management including radiographic evaluation and short-term complications were recorded. Forty-five dogs with a total of 47 PTMF identified and treated between 2007-2019 were included in this study. Six cases were managed with external coaptation alone. Forty-one cases were treated surgically with constructs including K-wires in different configurations, bone plate and screws, and external skeletal fixation. Of the cases managed conservatively, 4 developed complications, including bandage sores, diffuse osteopenia of the tarsus/metatarsus, and angular limb deformities. Surgical complications including pin migration necessitating removal, osteopenia, and screw placement in the proximal tibial growth plate or into the stifle joint were found in 16 cases. PTMF treated with surgery had a subjectively more predictable outcome compared to those treated with external coaptation alone. Conservative management may result in complications including development of excessive tibial plateau angle (TPA) as well as distal tibial valgus.

The article investigates the revitalization of the EU-Turkey migration diplomacy in the light of the 2016 EU-Turkey Statement. The Statement, which was aimed at stemming irregular migrations directed to the EU, contributed to strengthen the image of Turkey as a champion in hosting Syrian refugees and a trusted gatekeeper tasked with the control of the EU external borders. How has the management of a humanitarian crisis and massive migration flows affected post 2016 Turkey-EU migration diplomacy? The article contends that Turkey’s claim for moral superiority on the management of refugees despite the material weakness vis-à-vis the West has been employed to galvanize a coercive migration diplomacy and redefine Turkish approach to the EU migration regime. Keywords: Migration Diplomacy, Turkey, EU migration regime, Syrian refugees, transit migration

Transport properties of electron-doped cuprate Sr1−xLaxCuO2 thin films have been investigated as a function of doping. In particular, optimal- and over-doped samples were obtained by tuning the Sr:La stoichiometric ratio. Optimal-doped samples show a non-Fermi liquid behavior characterized by linear dependence of the resistivity from room temperature down to intermediate temperature (about 150–170 K). However, by approaching temperatures in the superconducting transition, a Fermi-liquid behavior-characterized by a T2-scaling law-was observed. Once established, the transition from a linear-T to a quadratic-T2 behavior was successfully traced back in over-doped samples, even occurring at lower temperatures. In addition, the over-doped samples show a crossover to a linear-T to a logarithmic dependence at high temperatures compatible with anti-ferromagnetic spin fluctuations dominating the normal state properties of electron-doped cuprates.

We grew Sr1-xLaxCuO2 thin films and SrCuO2/Sr0.9La0.1CuO2/SrCuO2 trilayers by reflection high-energy diffraction-calibrated layer-by-layer molecular beam epitaxy, to study their electrical transport properties as a function of the doping and thickness of the central Sr0.9La0.1CuO2 layer. For the trilayer samples, as already observed in underdoped SLCO films, the electrical resistivity versus temperature curves as a function of the central layer thickness show, for thicknesses thinner than 20 unit cells, sudden upturns in the low temperature range with the possibility for identifying, in the normal state, the T* and a T** temperatures, respectively, separating high-temperature linear behavior and low-temperature quadratic dependence. By plotting the T* and T** values as a function of TConset for both the thin films and the trilayers, the data fall on the same curves. This result suggests that, for the investigated trilayers, the superconducting critical temperature is the important parameter able to describe the normal state properties and that, in the limit of very thin central layers, such properties are mainly influenced by the modification of the energy band structure and not by interface-related disorder.

We investigate the influence of position, under large circular sputtering targets, on the final electrochemical performance of 35 mm diameter button solid oxide fuel cells with sputter-deposited Gadolinium doped Ceria barrier layers, positioned in order to almost cover the entirety of the area associated with a 120 × 80 mm2 industrial cell. We compare the results obtained via structural and morphological analysis to the Electrochemical Impedance Spectroscopy (EIS) measurements performed on the button cells, disentangling the role of different parameters. The Atomic Force Microscopy analysis makes it possible to observe a decrease in the roughness values from the peripheral to the central zones under the sputtering target, with peak-to-valley roughness values, respectively, decreasing from 380 nm to 300 nm, while Scanning Electron Microscopy and Energy Dispersive Spectroscopy show a dependence of the layer coverage from the position. The electrochemical performances of button cells with buffer layers of only 200 nm in thickness, and with negligible thickness gradients across them, show current density values of up to 478 mA/cm2 at 0.8 V and 650 °C, with an improvement of more than 67% with respect to button cells with standard (screen printed) buffer layers. These results point out the major influence exerted by parameters such as the thickness gradient and the coverage of the sputtered buffer layers in determining the final electrochemical performances.

Gene-expression programs modulated by transcription factors (TFs) mediate key developmental events. Here, we show that the synthetic transcriptional repressor (TR; ZF6-DB), designed to treat Rhodopsin-mediated autosomal dominant retinitis pigmentosa (RHO-adRP), does not perturb murine retinal development, while maintaining its ability to block Rho expression transcriptionally. To express ZF6-DB into the developing retina, we pursued two approaches, (i) the retinal delivery (somatic expression) of ZF6-DB by Adeno-associated virus (AAV) vector (AAV-ZF6-DB) gene transfer during retinogenesis and (ii) the generation of a transgenic mouse (germ-line transmission, TR-ZF6-DB). Somatic and transgenic expression of ZF6-DB during retinogenesis does not affect retinal function of wild-type mice. The P347S mouse model of RHO-adRP, subretinally injected with AAV-ZF6-DB, or crossed with TR-ZF6-DB or shows retinal morphological and functional recovery. We propose the use of developmental transitions as an effective mode to challenge the safety of retinal gene therapies operating at genome, transcriptional, and transcript levels.

Since the early 2000s, an increasing number of female religious officers have been employed by the Presidency of Religious Affairs (Diyanet) and regularly sent to European countries. Tasked with providing religious knowledge and moral support to women, their engagement epitomises Diyanet’s contribution to the AKP government’s wide-ranging international mission aimed at reaching Turkish citizens living abroad. To assess the significance of the activities organised for Turkish women migrated to Europe, the paper aims to answer the following questions: How does the ‘export’ of Diyanet female religious officers fit into Diyanet’s grip on international affairs? What is women’s contribution in this respect? And how do the activities of Diyanet women preachers in Europe reflect Turkey’s current diaspora policies? Drawing on ethnographic observation and interviews with the Diyanet female officers in Vienna and Stockholm mosques, this contribution concludes that Diyanet officers’ role and agency abroad is the result of a combination of two concomitant and interconnected factors The paper argues that female religious officers’ activities abroad should be included in a multifaceted reconfiguration of: i) the Diyanet’s long lasting international mission; ii) the role women play in the diffusion of religious knowledge and morality; iii) the boundaries between Turkey’s religious and diaspora policies.

Poor treatment outcomes are available for anorexia nervosa (AN) and treatment innovations are urgently needed. Recently, non-invasive neuromodulation tools have suggested to have potential for reducing an symptomatology targeting brain alterations. The objective of the study was to verify whether left anodal/right cathodal prefrontal cortex transcranial direct current stimulation (tDCS), may aid in altering/resetting inter-hemispheric balance in patients with AN, re-establishing control over eating behaviors. Twenty-three adolescents with an underwent a treatment as usual (AU), including nutritional, pharmacological, and psychoeducational treatment, plus 18 sessions of tDCS (TDCS+AU = n11; mean age = 13.9, SD = 1.8 years) or a family based therapy (FBT+AU = n12, mean age = 15.1, SD = 1.5 years). Psychopathological scales and the body mass index (BMI) were assessed before and after treatment. After 6 weeks of treatment, the BMI values increased only in the tDCS group, even at 1-month follow-up. Independently of the treatment, all participants improved in several psychopathological measures, included AN psychopathology and mood and anxiety symptoms. Our results demonstrated for the first time a specific effect of the left anodal/right cathodal tDCS treatment protocol on stable weight gain and a superiority compared to an active control treatment for adolescents with AN. Results were interpreted as a possible direct/indirect effect of tDCS in into some pathophysiological mechanisms of AN, involving the mesocortical dopaminergic pathways and the promotion of food intake. This pilot study opens new perspectives in the treatment of an in adolescence, supporting the targeted and beneficial effects of a brain-based treatment.

Head and neck manifestations of acquired immunodeficiency syndrome are among the most common complications of this disease. The sinonasal and oral manifestations are more common that the otologic and range from malignancies to infectious processes caused by both opportunistic and nonopportunistic organisms. We report the case of a nasopharyngeal mass of infectious etiology in a severely immunocompromised HIV infected patient. The patient was admitted with a presumptive diagnosis of infectious gastroenteritis and was found to have a nasopharyngeal mass. The mass was extending into the oropharynx and paravertebral soft tissues and was associated with extensive secretions causing near complete occlusion of the oropharynx. CT scan findings favored malignant verses infectious etiology. The surgical biopsy performed twice ruled out malignancy and the bacterial culture proved to be a pure growth of Pseudomonas aeruginosa. Pseudomonas can inhabit the nasopharynx and lower digestive tract, and is only occasionally associated with causing disease in non-susceptible patients but is a common infection in immunocompromised patients. To the best of our knowledge, and after considering the current literature, we believe this case is unique. We discuss this rare entity and its management. Clinicians should be aware of this potential life threatening condition in the HIV population and add P. aeruginosa infection to the differential diagnosis of an acute inflammatory nasopharyngeal mass.

The effectiveness of hyaluronic acid (HA), also called as hyaluronan, and its formulations on tissue regeneration and epidermal disease is well-documented. High-molecular-weight hyaluronan (HHA) is an efficient space filler that maintains hydration, serves as a substrate for proteoglycan assembly, and is involved in wound healing. Recently, an innovative hybrid cooperative complex (HCC) of high- and low-molecular-weight hyaluronan was developed that is effective in wound healing and bioremodeling. The HCC proposed here consisted of a new formulation and contained 1.6 ± 0.1 kDa HHA and 250 ± 7 kDa LHA (low molecular weight hyaluronic acid). We investigated the performance of this HCC in a novel in vitro HaCaT (immortalized human keratinocytes)/HDF (human dermal fibroblast) co-culture model to assess its ability to repair skin tissue lesions. Compared to linear HA samples, HCC reduced the biomarkers of inflammation (Transforming Growth Factor-β (TGF-β), Tumor Necrosis Factor receptor-α (TNF-α), interleukin-6 (IL-6), and interleukin-8 (IL-8)), and accelerated the healing process. These data were confirmed by the modulation of metalloproteases (MMPs) and elastin, and were compatible with a prospectively reduced risk of scar formation. We also examined the expression of defensin-2, an antimicrobial peptide, in the presence of hyaluronan, showing a higher expression in the HCC-treated samples and suggesting a potential increase in antibacterial and immunomodulatory functions. Based on these in vitro data, the presence of HCC in creams or dressings would be expected to enhance the resolution of inflammation and accelerate the skin wound healing process.

The use of a doped Ceria buffer layer and Physical Vapour Deposition (PVD) techniques for Solid Oxide Fuel Cells (SOFC) fabrication can limit the former, the formation of electrical insulating lanthanum, and strontium zirconates at the cathode/electrolyte interface, whereas the latter allows a better control of the materials interfaces. These effects allow for operation at intermediate temperature ranges. In this work, we study the structural and electrical properties of Gadolinium Doped Ceria (GDC) barrier layer deposited via the room temperature RF Sputtering technique on anode supported electrolytes and then annealed at high temperature. The crystal structure and the surface morphology of the GDC barrier layers have been analyzed and optimized varying the temperature ramp of the post-growth annealing procedure. The electrical behavior of the obtained samples has been investigated by Electrochemical Impedance Spectroscopy and compared to that of standard SOFC with screen-printed GDC barrier layers, the former showing a maximum high frequency and low frequency resistances reduction of about 50% and 46%, respectively, with respect to the latter at an operating temperature of 650 °C. The results clearly show an important improvement of SOFC performances when using sputter deposited GDC layers, linking the electrical properties to the structural and stoichiometric ones.

Apomyoglobin is an excellent example of a monomeric all α-helical globular protein whose folding pathway has been extensively studied and well characterized. Structural perturbation induced by denaturants or high temperature as well as amino acid substitution have been described to induce misfolding and, in some cases, aggregation. In this article, we review the molecular mechanism of the aggregation process through which a misfolded form of a mutated apomyoglobin aggregates at physiological pH and room temperature forming an amyloid fibril. The results are compared with data showing that either amyloid or aggregate formation occurs under particular denaturing conditions or upon cleavage of the residues corresponding to the C-terminal helix of apomyoglobin. The results are discussed in terms of the sequence regions that are more important than others in determining the amyloid aggregation process.

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The detection of a low serum phosphate level is not unusual in an Emergency Department, especially in clinical conditions linked to hyperventilation and subsequent respiratory alkalosis, asthma, sepsis, severe pain, anxiety. Symptoms of hypophosphatemia are typically not specific when the imbalance is not particularly severe, but if hyphophosphatemia does not resolve rhabdomyolisis, hemolysis, decreased tissue oxygenation and respiratory failure can be observed. Only recently some authors have pointed out that the level of serum phosphate in patient with anxiety and panic disorders can give information on the severity of the attacks as well on the clinical course of the disease. In a retrospective analysis on 599 case of hypophosphatemia observed in our ED, the percentage of case of panic disorders was particularly high among patients with lower phosphatemia. Therefore, we decided to examine this aspect closely, assessing if the determination of serum phosphate could be useful in the management of panic attacks at first approach in emergency room. Our observation are consistent with the statement that hypophosphatemia is one of the main clinical aspect of panic attack, and strongly support the hypothesis that hypophosphatemia correlates with the most severe symptoms of panic attack and should be itself considered as one of the most important aspect of this syndrome. Serum phosphate levels appear to mirror its clinical course, and can be used in the clinical setting of an Emergency Department, for the confirmation of a diagnosis of anxiety-panic disorder and as marker of the response to therapy

Due to their interesting ferroelectric, conductive and dielectric properties, in recent years, perovskite-structured materials have begun to attract increasing interest in the biosensing field. In this study, a strontium titanate perovskite layer (SrTiO3) has been synthesized on a platinum electrode and exploited for the development of an impedimetric label-free immunosensor for Escherichia coli O157:H7 detection. The electrochemical characterization of the perovskite-modified electrode during the construction of the immunosensor, as well as after the interaction with different E. coli O157:H7 concentrations, showed a reproducible decrease of the total capacitance of the system that was used for the analytical characterization of the immunosensor. Under optimized conditions, the capacitive immunosensor showed a linear relationship from to 1 to 7 log cfu/mL with a low detection limit of 1 log cfu/mL. Moreover, the atomic force microscopy (AFM) technique underlined the increase in roughness of the SrTiO3-modified electrode surface after antibody immobilization, as well as the effective presence of cells with the typical size of E. coli.

Neurodegenerative diseases are associated with misfolding and deposition of specific proteins, either intra or extracellularly in the nervous system. Advanced glycation end products (AGEs) originate from different molecular species that become glycated after exposure to sugars. Several proteins implicated in neurodegenerative diseases have been found to be glycated in vivo and the extent of glycation is related to the pathologies of the patients. Although it is now accepted that there is a direct correlation between AGEs formation and the development of neurodegenerative diseases, several questions still remain unanswered: whether glycation is the triggering event or just an additional factor acting on the aggregation pathway. To this concern, in the present study we have investigated the effect of glycation on the aggregation pathway of the amyloidogenic W7FW14F apomyoglobin. Although this protein has not been related to any amyloid disease, it represents a good model to resemble proteins that intrinsically evolve toward the formation of amyloid aggregates in physiological conditions. We show that D-ribose, but not D-glucose, rapidly induces the W7FW14F apomyoglobin to generate AGEs in a time-dependent manner and protein ribosylation is likely to involve lysine residues on the polypeptide chain. Ribosylation of the W7FW14F apomyoglobin strongly affects its aggregation kinetics producing amyloid fibrils within few days. Cytotoxicity of the glycated aggregates has also been tested using a cell viability assay. We propose that ribosylation in the W7FW14F apomyoglobin induces the formation of a cross-link that strongly reduces the flexibility of the H helix and/or induce a conformational change that favor fibril formation. These results open new perspectives for AGEs biological role as they can be considered not only a triggering factor in amyloidosis but also a player in later stages of the aggregation process.

Glycosaminoglycans (GAGs) are frequently associated with amyloid deposits in most amyloid diseases, and there is evidence to support their active role in amyloid fibril formation. The purpose of this study was to obtain structural insight into GAG-protein interactions and to better elucidate the molecular mechanism underlying the effect of GAGs on the amyloid aggregation process and on the related cytotoxicity. To this aim, using Fourier transform infrared and circular diochroism spectroscopy, electron microscopy and thioflavin fluorescence dye we examined the effect of heparin and other GAGs on the fibrillogenesis and cytotoxicity of aggregates formed by the amyloidogenic W7FW14 apomyoglobin mutant. Although this protein is unrelated to human disease, it is a suitable model for in vitro studies because it forms amyloid-like fibrils under physiological conditions of pH and temperature. Heparin strongly stimulated aggregation into amyloid fibrils, thereby abolishing the lag-phase normally detected following the kinetics of the process, and increasing the yield of fibrils. Moreover, the protein aggregates were harmless when assayed for cytotoxicity in vitro. Neutral or positive compounds did not affect the aggregation rate, and the early aggregates were highly cytotoxic. The surprising result that heparin induced amyloid fibril formation in wild-type apomyoglobin and in the partially folded intermediate state of the mutant, i.e., proteins that normally do not show any tendency to aggregate, suggested that the interaction of heparin with apomyoglobin is highly specific because of the presence, in protein turn regions, of consensus sequences consisting of alternating basic and non-basic residues that are capable of binding heparin molecules. Our data suggest that GAGs play a dual role in amyloidosis, namely, they promote beneficial fibril formation, but they also function as pathological chaperones by inducing amyloid aggregation.

Abstract The aim of this study was to investigate cumulus expansion, nuclear maturation and expression of connexin 43, cyclooxygenase-2 and FSH receptor transcripts in equine cumuli oophori during in vivo and in vitro maturation in the presence of equine FSH (eFSH) and precursors for hyaluronic acid synthesis. Equine cumulus-oocyte complexes (COC) were cultured in a control defined medium supplemented with eFSH (0 to 5 micrograms/ml), Fetal Calf Serum (FCS), precursors for hyaluronic acid synthesis or glutamine according to the experiments. After in vitro maturation, the cumulus expansion rate was increased with 1 microgram/ml eFSH, and was the highest with 20% FCS. It was not influenced by precursors for hyaluronic acid synthesis or glutamine. The expression of transcripts related to cumulus expansion was analyzed in equine cumulus cells before maturation, and after in vivo and in vitro maturation, by using reverse transcription-polymerase chain reaction (RT-PCR) with specific primers. Connexin 43, cyclooxygenase-2 (COX-2) and FSH receptor (FSHr) mRNA were detected in equine cumulus cells before and after maturation. Their level did not vary during in vivo or in vitro maturation and was influenced neither by FSH nor by precursors for hyaluronic acid synthesis. Results indicate that previously reported regulation of connexin 43 and COX-2 proteins during equine COC maturation may involve post-transcriptional mechanisms.