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The operationalisation of Responsible Research and Innovation is increasingly associated with ethical toolkits. However, scholars remain critical of those toolkits, often referring to them as theoretically problematic, toothless, or too instrumental. Moreover, toolkits imply ideological commitments that are not necessarily made explicit. In this scoping review, we analyse 127 tools designed for technology ethics as part of the RRI Project. We find that (1) these tools tend to frame responsibility as general training or aimed at the development phase of technologies, while monitoring is underrepresented. (2) These toolkits often lack substantive conceptualisations of ethics ignoring contested paradigms. (3) Emerging digital and biotechnologies are over-represented in relation to other socio-technical infrastructures, and (4) there is a risk of a PDF-ization of ethics, as most toolkits are materially constructed as reading material and checklists. We conclude by presenting prompt questions to reflectively reconsider how we design ethical toolkits for technology.

Several perennial snow and ice bodies have been recently studied and monitored in three mountain massifs across the Balkan Peninsula: Prokletije (Northern Albania), Durmitor (Montenegro) and Pirin (Bulgaria), the two glacierets in the Pirin Mountains until soon considered as southernmost of their kind in Europe. In September 2022 a relatively large snow field (about 1.5 ha) was discovered and described for a first time in the glacial cirque Gryka e Kazanit in the Nëmerçka Mountains of Southern Albania. The snow accumulation lies on 1550–1650 m a.s.l. below a vertical limestone cliff with a height of almost 1000 m. The site was visited again in November 2023, when the snow field diminished its size to less than 1 ha. At the same time, multi-annual firn layers were exposed under the last year snow, with depth at least several metres, and indications were observed of ice, buried in the debris cover below. Newest findings indicate that the studied snow-firn body is a glacieret similar to those found in the Pirin Mountains. On a longer-term, nine perennial snow/firm bodies on the Balkans resemble small glaciers rather than snow and ice patches at least under current climatic conditions. Situated on 40°08′ Northern latitude, the newly discovered Nemërçka Glacieret is nominated to be the southernmost glacier in Europe.

This research seeks to develop and test a rapid mapping approach using unmanned aerial vehicles (UAVs) and terrestrial laser scanning to provide precise, high-resolution spatial data for urban areas right after disasters. This mapping aims to support efforts to protect the population and infrastructure while analyzing the situation in affected areas. It focuses on flood-prone regions lacking modern hydrological data and where regular monitoring is absent. This study was conducted in resort villages and adjacent catchments in Bulgaria’s southern Black Sea coast with leading maritime tourism features, after a flash flood on 5 September 2023 caused human casualties and severe material damage. The resulting field data with a spatial resolution of 3 to 5 cm/px were used to trace the effects of the flood on topographic surface changes and structural disturbances. Flood simulation using UAV data and a digital elevation model was performed. The appropriateness of contemporary land use forms and infrastructure location in catchments is discussed. The role of spatial data in the analysis of genetic factors in risk assessment is commented on. The results confirm the applicability of rapid mapping in informing the activities of responders in a period of increased vulnerability following a flood. The results were used by Bulgaria’s Ministry of Environment and Water to analyze the situation shortly after the disaster.

Drought stress affects many aspects of plant biochemistry, with photosynthesis being one the most significantly impaired physiological processes. Melatonin is a natural antioxidant with growth-regulating properties in plants. Its diverse physiological functions have been extensively studied in recent decades. Changes in leaf gas exchange and chlorophyll a fluorescence parameters were investigated in young wheat plants (Triticum aestivum L.) cv. Fermer and cv. Gines which were characterized to differ in their responses to drought, with cv. Gines being more tolerant than cv. Fermer. The plants were subjected to drought for five days by withholding their water supply. Melatonin was applied as a root supplement to the irrigation water before or after the drought period. Analyses were performed before and at the end of the stress period, as well as during the recovery phase. Changes in leaf pigment content, photosynthetic rate, stomatal conductance, and transpiration, as well as some chlorophyll a fluorescence parameters, were recorded. Melatonin alone did not cause considerable changes in the measured traits. We found a significant decrease in leaf gas exchange parameters, Fv/Fm and Fv/F0 values, and leaf pigments due to drought, especially in cv. Fermer. The data show that the application of melatonin favorably influenced the efficiency of the photosynthetic apparatus under water deprivation and during plant recovery. The pre-treatment with melatonin maintained the photosynthesis-related parameters closer to the control levels during the stress period. Both melatonin treatments supported the recovery of photosynthesis when the water supply was restored and the post-drought treatment showed a similar but weaker effect than pre-drought treatment.

The urban heat island effect (UHI) is among the major challenges of urban climate, which is continuously intensifying its impact on urban life and functioning. Against the backdrop of increasingly prolonged heatwaves observed in recent years, practical questions about adaptation measures in cities are growing—questions that traditional meteorological monitoring can hardly answer adequately. On the other hand, UHI has long been the focus of research interest, but due to the technological complexity of providing accurate spatially referenced data at high spatial resolution and the requirement to survey at strictly defined parts of the day, information provision is becoming a major challenge. This is one of the main reasons why UHI research results are less often used directly in urban spatial planning. However, advances in geospatial technologies, including unmanned aerial systems (UASs), are providing more and more reliable tools that can be applied to achieve better and higher-quality information resources that adequately characterize the UHI phenomenon. This paper presents a developed and tested methodology for the rapid and efficient assessment and mapping of the effects of surface urban heat island (SUHI). It is entirely based on the integrated use of data from unmanned aerial systems (UAS)-based remote sensing methods, including thermal photogrammetry and GIS-based analysis methods. The study follows the understanding that correct SUHI research depends on a proper understanding of the urban geosystem, its spatial and structural heterogeneity, and its functional systems, which in turn can only be achieved by supporting the research process with accurate and reliable information resources. In this regard, the possibilities offered by the proposed methodological scheme for efficient geospatial registration of SUHI variations at the microscale, including the calculation of intra-urban SUHI intensity, are discussed in detail. The methodology builds on classical approaches for using local climate zoning (LCZ), adding capabilities for precise delineation of individual zone types and for geostatistical characterization of the urban surface heat island (SUHI). Finally, the proposed scheme is based on state-of-the-art technological tools that provide flexible and automated capabilities to investigate the phenomenon at microscales, including by enabling flexible observation of its dynamics in terms of heat wave manifestation and evolution. Results are presented from a series of sequential tests conducted on the largest residential area in Bulgaria’s capital city, Sofia, in terms of area and population, over a relatively long period from 2021 to 2024.

This study aims to scientifically justify the identification of suitable urban properties for urban green infrastructure (UGI) interventions to optimize its natural regulating functions for long-term pollution mitigation and secondary dust reduction. This study adheres to the perception that planning urban transformations to improve ambient air quality (AQ) requires a thorough understanding of urban structural heterogeneity and its interrelationship with the local microclimate. We apply an approach in which UGI and its potential multifunctionality are explored as a structural–functional element of urban local climatic zones. The same (100 × 100 m) spatial framework is used to develop place-based adapted solutions for intervention in UGI. A complex geospatial analysis of Burgas City, the second largest city (by area) in Bulgaria, was conducted by integrating 12 indicators to reveal the spatial disbalance of AQ regulation’ demand and UGI’s potential to supply ecosystem services. A total of 174 municipally owned properties have been identified, of which 79 are of priority importance, including for transport landscaping, inner-quarter spaces, and social infrastructure. Indicators of population density and location of social facilities were applied with the highest weight in the process of prioritizing sites. The study relies on public data and information from the integrated city platform of Burgas, in cooperation with the city’s government. The results have been discussed with stakeholders and implemented by the Municipality of Burgas in immediate greening measures in support of an ongoing program for Burgas Municipality AQ improvement.

The Nagoya Protocol is an international agreement adopted in 2010 (and entered into force in 2014) which governs access to genetic resources and the fair and equitable sharing of benefits from their utilisation. The agreement aims to prevent misappropriation of genetic resources and, through benefit sharing, create incentives for the conservation and sustainable use of biological diversity. While the equitable sharing of the benefits arising from the utilisation of genetic resources is a widely accepted concept, the way in which the provisions of the Nagoya Protocol are currently being implemented through national access and benefit-sharing legislation places significant logistical challenges on the control of transboundary livestock diseases such as foot-and-mouth disease (FMD). Delays to access FMD virus isolates from the field disrupt the production of new FMD vaccines and other tailored tools for research, surveillance and outbreak control. These concerns were raised within the FMD Reference Laboratory Network and were explored at a recent multistakeholder meeting hosted by the European Commission for the Control of FMD. The aim of this paper is to promote wider awareness of the Nagoya Protocol, and to highlight its impacts on the regular exchange and utilisation of biological materials collected from clinical cases which underpin FMD research activities, and work to develop new epidemiologically relevant vaccines and other diagnostic tools to control the disease.

AbstractAssessment and monitoring of health status in deteriorated forest stands in Gornata Koria and Chuprene Reserves in Western Balkan Range (Bulgaria) were conducted in 2017. An integrated approach (based on remote sensing technologies and terrestrial validation) was applied. A series of subsequent terrain observations were carried out in both protected areas mainly in dead or in poor health status stands. As a result, sixteen insect species were identified in Gornata Koria and nine pests in Chuprene Reserves. The predominant number of pests included bark beetles and weevils (Curculionidae), longhorn beetles (Cerambycidae), etc. Attacks caused by the European bark beetle (Ips typographus) formed the main disturbance in the Norway spruce forests in both reserves. Ten parasitic and ten saprophytic fungi were identified in the stands of the Norway spruce (Picea abies), silver fir (Abies alba) and European beech (Fagus sylvatica). Among the parasitic fungi, six species were identified as destructive (Armillaria sp., Heterobasidion annosum, Fomitopsis pinicola, Fomes fomentarius, Ramaria flava and Pholiota squarrosa).

The worldwide obesity and type 2 diabetes epidemics have led to an increase in nonalcoholic fatty liver disease (NAFLD). NAFLD covers a spectrum of hepatic pathologies ranging from simple steatosis to nonalcoholic steatohepatitis, characterized by fibrosis and hepatic inflammation. Nonalcoholic steatohepatitis predisposes to the onset of hepatocellular carcinoma (HCC). Here, we characterized the effect of a pharmacological activator of the intracellular energy sensor adenosine monophosphate–activated protein kinase (AMPK) on NAFLD progression in a mouse model. The compound stimulated fat oxidation by activating AMPK in both liver and skeletal muscle, as revealed by indirect calorimetry. This translated into an ameliorated hepatic steatosis and reduced fibrosis progression in mice fed a diet high in fat, cholesterol, and fructose for 20 weeks. Feeding mice this diet for 80 weeks caused the onset of HCC. The administration of the AMPK activator for 12 weeks significantly reduced tumor incidence and size. Conclusion: Pharmacological activation of AMPK reduces NAFLD progression to HCC in preclinical models.

This article presents the results of the thermal survey of the capital of Bulgaria (Sofia) carried out in August 2019, with the application of an unmanned aerial system (UAS). The study is based on the concept of local climate zones (LCZs), taking into account the influence of the features of land use/land cover and urban morphology on the urban climate. The basic spatial units used in the study are presented in the form of a regular grid consisting of 3299 cells with sides of 250 × 250 m. A total of 13 types of LCZs were identified, of which LCZs 6, 5, 8, 4, D, and A form the largest share. In the thermal imaging of the surface, a stratified sampling scheme was applied, which allowed us to select 74 cells, which are interpreted as representative of all cells belonging to the corresponding LCZ in the urban space. The performed statistical analysis of the thermal data allowed us to identify both the most thermally loaded zones (LCZs 9, 4, and 5) and the cells forming Urban Cool Islands (mainly in LCZs D and C). The average surface temperature in Sofia during the study period (in the time interval between 8:00 p.m. and 10:00 p.m.) was estimated at 20.9 °C, and between the different zones it varied in the range 17.2–25.1 °C. The highest maximum values of LST (27.9–30.6 °C) were registered in LCZ 4 and LCZ 5. The relation between the spatial structure of the urban thermal patterns and urban surface characteristics was also analyzed. Regression analysis confirmed the hypothesis that as the proportion of green areas increases, surface temperatures decrease, and, vice versa, as the proportion of built-up and impermeable areas increases, surface temperatures increase. A heat load map (via applying a z-transformation to standardize the temperature values), a map of the average surface temperature, and a map of the average intensity of the heat island on the surface were generated in the GIS environment. The results of the study adequately reflect the complex spatial model of the studied phenomenon, which gives grounds to conclude that the research approach used is applicable to similar studies in other cities.

Monitoring the structure parameters and damage to trees plays an important role in forest management. Remote-sensing data collected by an unmanned aerial vehicle (UAV) provides valuable resources to improve the efficiency of decision making. In this work, we propose an approach to enhance algorithms for species classification and assessment of the vital status of forest stands by using automated individual tree crowns delineation (ITCD). The approach can be potentially used for inventory and identifying the health status of trees in regional-scale forest areas. The proposed ITCD algorithm goes through three stages: preprocessing (contrast enhancement), crown segmentation based on wavelet transformation and morphological operations, and boundaries detection. The performance of the ITCD algorithm was demonstrated for different test plots containing homogeneous and complex structured forest stands. For typical scenes, the crown contouring accuracy is about 95%. The pixel-by-pixel classification is based on the ensemble supervised classification method error correcting output codes with the Gaussian kernel support vector machine chosen as a binary learner. We demonstrated that pixel-by-pixel species classification of multi-spectral images can be performed with a total error of about 1%, which is significantly less than by processing RGB images. The advantage of the proposed approach lies in the combined processing of multispectral and RGB photo images.

(1) Background: Persistent Helicobacter pylori infection is the most important risk factor for gastric cancer. The urokinase receptor (uPAR) is upregulated in lesions harboring cancer invasion and inflammation. Circumstantial evidence tends to correlate H. pylori colonization with increased uPAR expression in the human gastric epithelium, but a direct causative link has not yet been established in vivo; (2) Methods: In a mouse model of H. pylori-induced gastritis, we investigated the temporal emergence of uPAR protein expression in the gastric mucosa in response to H. pylori (SS1 strain) infection; (3) Results: We observed intense uPAR immunoreactivity in foveolar epithelial cells of the gastric corpus due to de novo synthesis, compared to non-infected animals. This uPAR induction represents a very early response, but it increases progressively over time as do infiltrating immune cells. Eradication of H. pylori infection by antimicrobial therapy causes a regression of uPAR expression to its physiological baseline levels. Suppression of the inflammatory response by prostaglandin E2 treatment attenuates uPAR expression. Notwithstanding this relationship, H. pylori does induce uPAR expression in vitro in co-cultures with gastric cancer cell lines; (4) Conclusions: We showed that persistent H. pylori colonization is a necessary event for the emergence of a relatively high uPAR protein expression in murine gastric epithelial cells.

Carcinoma-associated fibroblasts are key contributors of the tumor microenvironment that regulates carcinoma progression. They consist of a heterogeneous cell population with diverse origins, phenotypes, and functions. In the present report, we have explored the contribution of bone marrow (BM)-derived cells to generate different fibroblast subsets that putatively produce the matrix metalloproteinase 13 (MMP13) and affect cancer cell invasion. A murine model of skin carcinoma was applied to mice, irradiated, and engrafted with BM isolated from green fluorescent protein (GFP) transgenic mice. We provide evidence that one third of BM-derived GFP+ cells infiltrating the tumor expressed the chondroitin sulfate proteoglycan NG2 (pericytic marker) or α-smooth muscle actin (α-SMA, myofibroblast marker), whereas almost 90% of Thy1+ fibroblasts were originating from resident GFP-negative cells. MMP13producing cells were exclusively α-SMA+ cells and derived from GFP+ BM cells. To investigate their impact on tumor invasion, we isolated mesenchymal stem cells (MSCs) from the BM of wild-type and MMP13-deficient mice. Wild-type MSC promoted cancer cell invasion in a spheroid assay, whereas MSCs obtained from MMP13-deficient mice failed to. Our data support the concept of fibroblast subset specialization with BM-derived α-SMA+ cells being the main source of MMP13, a stromal mediator of cancer cell invasion.

In prostate cancers (PCa), the formation of malignant stroma may substantially influence tumor phenotype and aggressiveness. Thus, the impact of the orthotopic and subcutaneous implantations of hormone-sensitive (H), hormone-insensitive (HI), anaplastic (AT1) Dunning PCa in rats on growth, microcirculation, metabolism was investigated. For this purpose, dynamic contrast-enhanced magnetic resonance imaging and 1H magnetic resonance spectroscopy ([1H]MRS) were applied in combination with histology. Consistent observations revealed that orthotopic H tumors grew significantly slower compared to subcutaneous ones, whereas the growth of HI and AT1 tumors was comparable at both locations. Histologic analysis indicated that glandular differentiation and a close interaction of tumor cells and smooth muscle cells (SMC) were associated with slow tumor growth. Furthermore, there was a significantly lower SMC density in subcutaneous H tumors than in orthotopic H tumors. Perfusion was observed to be significantly lower in orthotopic H tumors than in subcutaneous H tumors. Regional blood volume and permeability-surface area product showed no significant differences between tumor models and their implantation sites. Differences in growth between subcutaneous and orthotopic H tumors can be attributed to tumor-stroma interaction and perfusion. Here, SMC, may stabilize glandular structures and contribute to the maintenance of differentiated phenotype.

The objective of the present study was to confirm the expression and localisation pattern of the urokinase-type plasminogen activator receptor (uPAR) focusing on its possible clinical relevance in patients with urothelial neoplasia of the bladder. uPAR is a central molecule in tissue remodelling during cancer invasion and metastasis and is an established prognostic marker in various cancer diseases other than bladder cancer. Formalin-fixed and paraffin-embedded tumour-tissue blocks from 186 patients treated with radical cystectomy were analysed. uPAR expression was scored as either negative or positive as well as by the actual score. Separate scores were obtained for cancer cells, macrophages and myofibroblasts at the invasive front and in tumour core. We were able to confirm, in an independent patient cohort, the tissue expression and localisation pattern of uPAR as investigated by Immunohistochemistry as well as a significant association between uPAR positivity and increasing tumour stage and tumour grade. This demonstrates the robustness of our previous and current findings. In addition the association between uPAR positive myofibroblasts and poor survival was reproduced. The highest hazard ratios for survival were seen for uPAR positive myofibroblasts both at the invasive front and in tumour core. Evaluating uPAR expression by the actual score showed a significant association between uPAR positive myofibroblasts in tumour core and an increased risk of cancer specific mortality. Our investigations have generated new and valuable biological information about the cell types being involved in tumour invasion and progression through the plasminogen activation system.