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Skin defects of the total nasal columella can significantly impact both nasal respiratory function and aesthetics. The reconstruction of total columella is a complex process and represents a significant challenge for plastic surgeons. Various factors can cause the loss of the columella. Numerous columella reconstruction procedures have been proposed, each with their own set of advantages and disadvantages. The main issues to address include the need for regional flaps from the forehead or nasofacial sulcus, a long pedicle to reach the columella, and the double angular folding that causes a risk of malnutrition or venous congestion. Additionally, using horizontal nasolabial flaps may lead to deformation of the upper lip.

This study aims to make a plant expression vector with an RBD-T4-Linker-C5a sequence that codes for the SARS-CoV-2 antigen and an A3Dsp signal peptide from the rice 3D amylase gene located before the RBD. Methods of molecular cloning were applied in this study. The plant expression vector pNHL22 harboring the RBD-T4-Linker-C5a sequence was successfully established and conjugated into Agrobacterium tumefaciens LBA4404 by triparental mating. Bacteria A. tumefaciens containing the RBD-T4-Linker-C5a sequence are now ready for genetic transformation into the Nicotiana benthamiana plant for future applications.

Background: Facial nerve palsy is one of the most common complications in parotid gland surgery. This report evaluates the effectiveness of applying Tumescent solution for preserving the facial nerve in parotidectomy. Material and methods: Prospective and descriptive clinical study on 34 patients undergoing parotidectomy with facial nerve preservation. Before skin incision, 5–10 min, we injected 100–200 ml of the Tumescent solution into the surgical area. We recorded the surgical method, tumor size, length of surgery, pathological results and facial nerve dysfunction. All patients were followed for a period ranging from 6 to 24 months. Results: There were 14 patients with malignant tumors and 20 patients with benign tumors. The length of surgery lasted from 90 to 180 min, with an average of 126.8 min. The number of patients having temporary facial paralysis was 22 cases (64.7%), recovery time ranged from 1 week to 6 months, and no permanent facial paralysis cases were recorded. The clinical occurrence of Frey's syndrome was five cases (14.7%). Conclusions: The application of Tumescent solution for preserving facial nerves in parotidectomy could minimize nerve injury and shorten the length of surgery.

Cryptotia is a rare congenital auricular deformity among Caucasians but more common in Asians. Various operative techniques have been described, such as skin graft, V-Y plasty, Z plasty, and islands skin flap. Among those techniques, V-Y plasty has many advantages and is indicated for severe cases. However, several problems remain when this method is adopted, such as conspicuous scars and lowered hairline. To overcome these problems, the authors have developed a new technique, multiple V-Y advancement modification, which stitches the skin flaps together to a tight, discrete central focal point. This technique has the advantage of hiding scars behind the earlobe, providing enough skin for the deformed areas, minimizing the condition of the hairline being lowered and auriculocephalic sulcus depth.

Coastal erosion and accretion along the Quang Nam coast in Vietnam have been increasing in recent years, causing negative impacts on the inhabitants and local ecology. The Cua Lo estuary in Nui Thanh district has a complex hydrodynamic regime owing to its connection with two estuaries and three different tributaries. Therefore, a detailed study of the mechanisms and processes of these phenomena is crucial to understand the potential impact of a proposed 50,000-ton cargo port. In this study, the Delft3D model is employed to evaluate the morpho-dynamic changes in the area of Cua Lo under monsoon wave climate, storm, and flood conditions both before and after port and navigation channel construction. Results indicate that in the absence of the port, tidal currents and waves during monsoon storms cause significant erosion on the south bank and accretion on the north bank. Furthermore, the GenCade model is utilized to predict the future shoreline changes after the construction of two jetties. The model reveals that after 50 years of operation, the shoreline modifications will extend 449 m towards the sea, in comparison to natural conditions. However, the design of the northern jetty will ensure safe and proper operation without impacting the navigation channel. This study offers valuable insights into the morphological changes in the Cua Lo area and their potential implications, which can aid in the development of sustainable coastal management strategies for the region.

Porcine epidemic diarrhea virus (PEDV), a member of the Coronaviridae family has become increasingly probelmatic in the pig farming industry. Currently, there are no effective, globally applicable vaccines against PEDV. Here, we tested a recombinant PEDV vaccine candidate based on the expression of the core neutralising epitope (COE) of PEDV conjugated to polymeric immunoglobulin G scaffold (PIGS) in glycoengineered Nicotiana benthamiana plants. The biological activity of COE-PIGS was demonstrated by binding to C1q component of the complement system, as well as the surface of antigen-presenting cells (APCs) in vitro. The recombinant COE-PIGS induced humoral and cellular immune responses specific for PEDV after both systemic and mucosal vaccination. Altogether, the data indicated that PEDV antigen fusion to poly-Fc could be a promising vaccine platform against respiratory PEDV infection.

The present work aims to establish an efficient protocol for in vitro regeneration of peanut (Arachis hypogaea) cultivar L14. The study showed that de-embryonated cotyledon was a suitable explant for shoot multiplication on MS medium containing 4 mg/L BAP. The highest number of shoots per explant obtained after 4 weeks of culture was up to 6.8 shoots. Shoots in vitro were able to produce a large number of approximately 11 roots on MS medium supplemented with 0.5 mg/L NAA. These results will be very useful in establishing an in vitro regeneration protocol for peanut cultivar L14 during gene transfer in the next studies to improve their disease resistance.

The present work aims to establish an efficient protocol for in vitro regeneration of peanut (Arachis hypogaea) cultivar L14. The study showed that de-embryonated cotyledon was a suitable explant for shoot multiplication on MS medium containing 4 mg/L BAP. The highest number of shoots per explant obtained after 4 weeks of culture was up to 6.8 shoots. Shoots in vitro were able to produce a large number of approximately 11 roots on MS medium supplemented with 0.5 mg/L NAA. These results will be very useful in establishing an in vitro regeneration protocol for peanut cultivar L14 during gene transfer in the next studies to improve their disease resistance.

Background: Heavy metal contamination and related risks for the environment and human health are matters of increasing concern. Methods: The levels of 4 heavy metals (Cr, Cd, Pb, and As) were evaluated in 2 water types (surface and well), 4 types of seafood (tiger shrimp, stuffed snail, snake-head fish, and catfish), and 27 types of vegetables (12 leafy vegetables, 4 pea plants, 4 tuber vegetables, and 7 herbs) that are commonly consumed in northern coastal communes located in Vietnam. Atomic absorption spectrometry was employed for quantification. Results: The mean concentrations of heavy metals detected in water, seafood, and vegetable samples exceeded the national permitted standards and World Health Organization (WHO) recommendation values by at least 2-fold, 2.5-fold, and 5-fold for surface water, vegetables, and well water, respectively. The concentrations of all 4 heavy metals detected in seafood samples were higher than the standards. The levels of heavy metals decreased with increasing distance between the sample collection point and the pollution source. Conclusions: This is the first report of heavy metal contamination of common sources of food and water in the northern coastal area of Vietnam. Significantly, the concentrations of heavy metals detected in study samples exceeded the regulatory limits. These results underscore the importance of continued monitoring and the development of intervention measures to ensure that the quality of food and water meets established standards and protects the health of the local population.

Tidal inlets along the central coast of Vietnam are located in a microtidal, wave-dominated coastal environment. In addition, the Vietnam coast is highly influenced by the seasonal monsoon regime, which is characterized by large northeast waves from October to March and calm southeast waves from April to September every year. Consequently, the tidal inlet entrance morphologies often suffer from a dynamic seasonal evolution due to distinct differences in the direction of wave-induced longshore sediment transport (LST) between the two monsoon seasons. The migration or closure of tidal inlets causes a lot of problems for socio-economic development in the region since these are the main reasons leading to an increase in the risk of coastal flooding and the obstruction of navigation. This paper presents a comprehensive study of the morphological evolutions of natural tidal inlets on the central coast of Vietnam using long-term remote sensing data sets and by the Delft3D numerical model. Surprisingly, the estimated LST rates from the former method are in an order of magnitude agreement with the results from the latter one for all of the areas in this study. Based on the conservation equation for sand and comprehensive data collection, a new simple empirical formula for predicting the sand spit elongation rate as a function of the sand spit width is developed. Although the breaching of sand spit might happen during an extreme flood event at some tidal inlets, the growth rate of the spit before and after the breaching is almost unchanged. These findings are very useful information for supporting the local coastal authorities to find better management solutions in terms of sustainable development.