1. Subclassification of B-acute lymphoblastic leukemia according to age, immunophenotype and microenvironment, predicts MRD risk in Mexican children from vulnerable regions
- Author
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Rubí Romo-Rodríguez, Gabriela Zamora-Herrera, Jebea A. López-Blanco, Lucero López-García, Arely Rosas-Cruz, Laura Alfaro-Hernández, César Omar Trejo-Pichardo, Dulce Rosario Alberto-Aguilar, Diana Casique-Aguirre, Armando Vilchis-Ordoñez, Juan Carlos Solis-Poblano, Lilia Adela García-Stivalet, Vanessa Terán-Cerqueda, Nuria Citlalli Luna-Silva, Miguel Ángel Garrido-Hernández, Lena Sarahí Cano-Cuapio, Karen Ayala-Contreras, Fabiola Domínguez, María de los Ángeles del Campo-Martínez, Gerardo Juárez-Avendaño, Juan Carlos Balandrán, Sonia Mayra Pérez-Tapia, Carlos Fernández-Giménez, Pedro A. Zárate-Rodríguez, Enrique López-Aguilar, Aurora Treviño-García, Célida Duque-Molina, Laura C. Bonifaz, Juan Carlos Núñez-Enríquez, Mariana Cárdenas-González, Elena R. Álvarez-Buylla, Dalia Ramírez-Ramírez, and Rosana Pelayo
- Subjects
tumor microenvironment ,risk stratification ,ProB-ALL ,measurable residual disease (MRD) ,Mexican children ,acute leukemia ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
IntroductionThe decisive key to disease-free survival in B-cell precursor acute lymphoblastic leukemia in children, is the combination of diagnostic timeliness and treatment efficacy, guided by accurate patient risk stratification. Implementation of standardized and high-precision diagnostic/prognostic systems is particularly important in the most marginalized geographic areas in Mexico, where high numbers of the pediatric population resides and the highest relapse and early death rates due to acute leukemias are recorded even in those cases diagnosed as standard risk.MethodsBy using a multidimensional and integrated analysis of the immunophenotype of leukemic cells, the immunological context and the tumor microenvironment, this study aim to capture the snapshot of acute leukemia at disease debut of a cohort of Mexican children from vulnerable regions in Puebla, Oaxaca and Tlaxcala and its potential use in risk stratification.Results and discussionOur findings highlight the existence of a distinct profile of ProB-ALL in children older than 10 years, which is associated with a six-fold increase in the risk of developing measurable residual disease (MRD). Along with the absence of CD34+ seminal cells for normal hematopoiesis, this ProB-ALL subtype exhibited several characteristics related to poor prognosis, including the high expression level of myeloid lineage markers such as MPO and CD33, as well as upregulation of CD19, CD34, CD24, CD20 and nuTdT. In contrast, it showed a trend towards decreased expression of CD9, CD81, CD123, CD13, CD15 and CD21. Of note, the mesenchymal stromal cell compartment constituting their leukemic niche in the bone marrow, displayed characteristics of potential suppressive microenvironment, such as the expression of Gal9 and IDO1, and the absence of the chemokine CXCL11. Accordingly, adaptive immunity components were poorly represented. Taken together, our results suggest, for the first time, that a biologically distinct subtype of ProB-ALL emerges in vulnerable adolescents, with a high risk of developing MRD. Rigorous research on potential enhancing factors, environmental or lifestyle, is crucial for its detection and prevention. The use of the reported profile for early risk stratification is suggested.
- Published
- 2024
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