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Purpose To evaluate the safety and efficacy of long vascular sheaths for transfemoral neuroendovascular procedures in children. Materials and Methods A retrospective evaluation of transfemoral neuroendovascular procedures in children 15 kg subcohorts. There were no aorto-femoro-iliac or limb-ischemic complications. Conclusion Long vascular sheaths without short femoral sheaths can be safely used for pediatric neuroendovascular procedures as they effectively increase inner diameter access without increasing the outer sheath diameter. This property increases the range of devices used and intracranial techniques that can be safely performed without arterial compromise, thus increasing the repertoire of the neurointerventionist.

Sonic Hedgehog medulloblastoma (Shh-MB) comprises four subtypes each with distinct clinical traits. Here the authors characterize the genome, transcriptome, and methylome of Shh-MB subtypes, revealing a complex fusion landscape and the molecular convergence of MYCN and cAMP signaling pathways.

Tumor-initiating cells are a subpopulation in aggressive cancers that exhibit traits shared with stem cells, including the ability to self-renew and differentiate, commonly referred to as stemness. In addition, such cells are resistant to chemo- and radiation therapy posing a therapeutic challenge. To uncover stemness-associated functions in glioma-initiating cells (GICs), transcriptome profiles were compared to neural stem cells (NSCs) and gene ontology analysis identified an enrichment of Ca2+ signaling genes in NSCs and the more stem-like (NSC-proximal) GICs. Functional analysis in a set of different GIC lines regarding sensitivity to disturbed homeostasis using A23187 and Thapsigargin, revealed that NSC-proximal GICs were more sensitive, corroborating the transcriptome data. Furthermore, Ca2+ drug sensitivity was reduced in GICs after differentiation, with most potent effect in the NSC-proximal GIC, supporting a stemness-associated Ca2+ sensitivity. NSCs and the NSC-proximal GIC line expressed a larger number of ion channels permeable to potassium, sodium and Ca2+. Conversely, a higher number of and higher expression levels of Ca2+ binding genes that may buffer Ca2+, were expressed in NSC-distal GICs. In particular, expression of the AMPA glutamate receptor subunit GRIA1, was found to associate with Ca2+ sensitive NSC-proximal GICs, and decreased as GICs differentiated along with reduced Ca2+ drug sensitivity. The correlation between high expression of Ca2+ channels (such as GRIA1) and sensitivity to Ca2+ drugs was confirmed in an additional nine novel GIC lines. Calcium drug sensitivity also correlated with expression of the NSC markers nestin (NES) and FABP7 (BLBP, brain lipid-binding protein) in this extended analysis. In summary, NSC-associated NES+/FABP7+/GRIA1+ GICs were selectively sensitive to disturbances in Ca2+ homeostasis, providing a potential target mechanism for eradication of an immature population of malignant cells.

Cell surface proteins have a wide range of biological functions, and are often used as lineage-specific markers. Antibodies that recognize cell surface antigens are widely used as research tools, diagnostic markers, and even therapeutic agents. The ability to obtain broad cell surface protein profiles would thus be of great value in a wide range of fields. There are however currently few available methods for high-throughput analysis of large numbers of cell surface proteins. We describe here a high-throughput flow cytometry (HT-FC) platform for rapid analysis of 363 cell surface antigens. Here we demonstrate that HT-FC provides reproducible results, and use the platform to identify cell surface antigens that are influenced by common cell preparation methods. We show that multiple populations within complex samples such as primary tumors can be simultaneously analyzed by co-staining of cells with lineage-specific antibodies, allowing unprecedented depth of analysis of heterogeneous cell populations. Furthermore, standard informatics methods can be used to visualize, cluster and downsample HT-FC data to reveal novel signatures and biomarkers. We show that the cell surface profile provides sufficient molecular information to classify samples from different cancers and tissue types into biologically relevant clusters using unsupervised hierarchical clustering. Finally, we describe the identification of a candidate lineage marker and its subsequent validation. In summary, HT-FC combines the advantages of a high-throughput screen with a detection method that is sensitive, quantitative, highly reproducible, and allows in-depth analysis of heterogeneous samples. The use of commercially available antibodies means that high quality reagents are immediately available for follow-up studies. HT-FC has a wide range of applications, including biomarker discovery, molecular classification of cancers, or identification of novel lineage specific or stem cell markers.

BACKGROUND:Rett Syndrome (RTT) is an Autism Spectrum Disorder and the leading cause of mental retardation in females. RTT is caused by mutations in the Methyl CpG-Binding Protein-2 (MECP2) gene and has no treatment. Our objective is to develop viral vectors for MECP2 gene transfer into Neural Stem Cells (NSC) and neurons suitable for gene therapy of Rett Syndrome. METHODOLOGY/PRINCIPAL FINDINGS:We generated self-inactivating (SIN) retroviral vectors with the ubiquitous EF1alpha promoter avoiding known silencer elements to escape stem-cell-specific viral silencing. High efficiency NSC infection resulted in long-term EGFP expression in transduced NSC and after differentiation into neurons. Infection with Myc-tagged MECP2-isoform-specific (E1 and E2) vectors directed MeCP2 to heterochromatin of transduced NSC and neurons. In contrast, vectors with an internal mouse Mecp2 promoter (MeP) directed restricted expression only in neurons and glia and not NSC, recapitulating the endogenous expression pattern required to avoid detrimental consequences of MECP2 ectopic expression. In differentiated NSC from adult heterozygous Mecp2(tm1.1Bird)+/- female mice, 48% of neurons expressed endogenous MeCP2 due to random inactivation of the X-linked Mecp2 gene. Retroviral MECP2 transduction with EF1alpha and MeP vectors rescued expression in 95-100% of neurons resulting in increased dendrite branching function in vitro. Insulated MECP2 isoform-specific lentiviral vectors show long-term expression in NSC and their differentiated neuronal progeny, and directly infect dissociated murine cortical neurons with high efficiency. CONCLUSIONS/SIGNIFICANCE:MeP vectors recapitulate the endogenous expression pattern of MeCP2 in neurons and glia. They have utility to study MeCP2 isoform-specific functions in vitro, and are effective gene therapy vectors for rescuing dendritic maturation of neurons in an ex vivo model of RTT.

Abstract How and why climate change opinions vary within countries at a small geographic scale is rarely investigated. Previous research has focused on public opinions at the individual or national level, leaving local differences within countries and their underlying factors largely unexplored. The lack of research at subnational levels is problematic, as adaptation and mitigation policies depend on collective support and action involving multiple stakeholders at the local scale. It is thus crucial to identify geographic differences in climate change opinions and to unravel their determinants at a fine-grained local scale. We examine public CCOs across 4,667 municipalities in Germany by relying on a representative survey of households. Here we show substantial and systematic differences in public climate change opinions across locations that manifest between urban vs. rural and prospering vs. declining areas. Besides these geographic features, more complex historical and cultural differences between places play an important role.

Abstract Narratives and stories are important communication tools and as such essential subjects of social geography. This paper analyses the retelling of Greta Thunberg’s sailing trip across the Atlantic to the Climate Action Summit in New York in 2019 in leading German newspapers and magazines and how her intentions are transformed through this reporting into different narratives. The research mainly focuses on examining the influence of space and place, as geographical research has revealed that spatial determinants are crucial in risk communication and knowledge generation on climate change but have yet to be studied considering stories. The paper, therefore, extends the story-based approach from communication sciences by geographical research on the role of space and place in action-based stories. Therefore, the Narrative Policy Framework (NPF) is used to decode the spatial environment in narratives as an active element that shapes the narrative, and the way characters can (inter)act within these settings. The paper further develops the NPF framework through a geographical lens by focusing particularly on the selection options of spaces for social interactions and affective bonds. Thus, it becomes evident how spatial contexts and environments shape the interactions between individuals and crucially influence the types of narratives that emerge.

Buchrezension.

Within the context of climate change in sub-Saharan Africa, trust in institutions is an important prerequisite to implement climate change adaptation and mitigation policies. There is a lack of systematic investigation of the relationship between climate change awareness, conflicts, and trust in institutions. We address this pressing research gap based on a regression analysis with trust in institutions as dependent variables, and climate change awareness and violence as independent variables drawing on Afrobarometer individual data and aggregated data on armed conflicts. Our main findings indicate that trust in institutions in sub-Saharan Africa is influenced by both the individual climate change awareness and the violence context. First, we find a negative relationship between those who are aware of climate change and trust in institutions. Second, we observe a socio-economic divide: young, urban and educated parts of the population as well as those who feel their ethnic group is treated unfairly do not trust institutions. Third, we see a regional divide: those far from political centres are not aware of climate change, and those close to the political centres do not trust institutions.

Climate change perceptions interact with how climate change is portrayed in the news, which is now increasingly accessed via social media platforms. While their effects on climate change awareness have been documented, it is less clear to what extent news consumed via social media platforms influences perceived climate change efficacy, which refers to the belief that one is able to make a difference in the fight against climate change. Our paper investigates the relationship between internet use, news received via social media, and perceived climate change efficacy in Europe, by using multilevel regression that shows the effects on individual, national and regional level. We find that there are modest differences between perceived climate change efficacy within our European sample and that on aggregated, national level Facebook negatively correlates with perceived climate change efficacy. Furthermore, regions with high participation in social media, show lower perceived climate change efficacy. Our multi-level research design thus puts new insights into the spatial manifestation of climate change opinions in the context of a digital geography interested in exploring differences in the effects of digital media uses.

The transition from industrial to post-industrial society has changed the conditions for labour protest in Europe fundamentally. In this paper recent forms of labour protest are explored in two European capital cities, Berlin and Budapest. In the context of growing job insecurity, flexibilisation and fragmentation of labour markets, the motivations and class positions of protest participants are scrutinised, along with the diversified geographies of protest events. Building on empirical results by means of a survey based on structured mini-interviews, the paper argues, first, that a fragmentation of labour protests on May 1st is observable. This fragmentation is driven by an overall change of Labour Day celebrations from trade union oriented demonstrations towards segmented party zones of protest in both cities. Second, neither traditional forms of labour protest is nor newly created, more festive forms of labour celebrations attract a significant proportion of people suffering from precarity or unemployment. Thus, most marginalised people in the service-dominated economy do not have a voice in labour protests today.

There is a need for semi-autonomous systems capable of performing both automated tasks and supervised maneuvers. When dealing with multiple robots or robots with high complexity (such as humanoids), we face the issue of effectively coordinating operators across robots. We build on our previous work to present a methodology for designing trajectories and policies for robots such that a few operators can supervise multiple robots. Specifically, we: (1) Analyze the complexity of the problem, (2) Design a procedure for generating policies allowing operators to oversee many robots, (3) Present a method for designing policies and robot trajectories to allow operators to oversee multiple robots, and (4) Include both simulation and hardware experiments demonstrating our methodologies.

The utilization of urban planning practices for political purposes has become an issue of planning theory in the last decades. Until the 1970s town planning was considered to be a neutral and rational instrument for the purpose of spatial regulation and administration as well as the general improvement of living conditions. Only recently with the rise of communicative planning theory this perspective has been complemented by critical perceptions of the political and ideological role of planning practices within society, their context-dependence, legitimization, normative purposes and also its interlinkages to individual aims as well as political and economic power. Jerusalem, the (partly occupied) capital of Israel, is a prototypic example of the utilization of urban planning for (even geo) political ends. This article is based on qualitative fieldwork and aims to scrutinize the scalar political consequences of various urban planning practices in Jerusalem. We undertake an analysis of the reciprocal relations of local, national, and geostrategic interests as well as the resulting actions by the relevant stakeholders. Theoretically informed by postcolonial geographies and performative approaches the paper concludes, that urban planning in Jerusalem is a highly political instrument that is strongly involved in the performative implementations of particular geographic imaginations of Jerusalem as a united capital of the state of Israel and historic as well as religious symbol of the Jewish nation. Hence urban planning in Jerusalem is an active element in the enforcement of hegemonic political interests which reach beyond the local context of Eastern Jerusalem.

the paper discusses the two theoretical concepts super-gentrification and metropolitan habitus established by British gentrification research. Both concepts are more or less unknown in German urban geography. Super-gentrification is looking at the gentrification of already gentrified urban neighbourhoods. Metropolitan habitus explains the affinity of specific social groups for very specific neighbourhoods in big cities. the paper starts with criticising both concepts for their missing explanation of influences of the qualities of urban space on habitus and super-gentrification. Secondly,the paper introduces the concept of residential capital, be un- derstood as inclusion of spatial qualities in social practices. in doing so, the theoretical gap of metropolitan habitus and super-gentrification can be bridged. In conclusion, the paper identifies some research questions for German urban geo- graphy that might be answered with the help of metropoli- tan habitus, super-gentrification and embedded residential capital.

The paper presents the thesis that the administration should be the target for innovation research. In the context of the promotion of innovations the administration deals with a threefold paradox. Innovations are non-intendable, the administration forms a non-innovative environment and the administration is dependent on majority decisions. Using the Free Hanseatic City of Bremen as a case study the paper scrutinizes the internal handling of these obstacles of the administration. As a main empirical finding it shows up that presentation and delegation are the main methods the administration uses to encounter its own endogenous resistances in the context of the promotion of innovation.

Because of their constitution, the usage of performative techniques in qualitative social research must deal with a paradox. Acting as performance takes place in the present and it takes place just once. One result of this is that every representation of a performance be it as text, discussion or film refers to the past. Performative social research solves this paradox by conceptualising performance as a kind of liminal phase of a ritual. Our thesis is that by simple outsourcing the problem of present in the theory of ritual, performative techniques commit the logical mistake of genetic fallacy, i.e., the mistake of forgetting that the primary value or meaning of an event has no necessary connections with its genesis in history. Therefore, a new methodology for qualitative social research after the performative turn requires a theoretical position which does not fall back to a position of causality as the temporal consequence of a cause and effect, as maintained by ritual theory. In this essay we suggest a "non-representational theory" for this venture, and point out how a methodology for qualitative research could be constituted "after" the performative turn. URN: urn:nbn:de:0114-fqs0802558