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Objective. To determine spectrum and prevalence of genetic determinants of macrolide and fluoroquinolone resistance in Mycoplasma genitalium from patient in dermatovenerology clinics of two Russian megalopolises: Moscow and St. Petersburg in 2021–2024. Materials and Methods. A total of 530 M. genitalium DNA-positive specimens (urethral and cervical swabs) obtained from men and women from the STD clinic in St. Petersburg and Сlinical facilities of the Moscow Scientific and Practical Center for Dermatovenerology and Cosmetology of the Moscow Department of Health Care were analyzed. Screening in local laboratories was performed using commercially available kits for isolation and detection of M. genitalium DNA. Primary screening for resistance-associated mutations was carried out using the previously developed protocol of PCR-RT (allows detecting any nucleotide substitutions in the 23S rRNA gene and parC gene) [1]. The results were confirmed using Sanger sequencing [2]. The study results are presented as an open project on the AMRcloud platform (https://amrcloud.net/ru/project/demares/). Results. 185 and 345 M. genitalium-positive samples from St. Petersburg and Moscow, respectively, showed the presence of a specific signal in both the 23S rRNA gene and parC gene in primary screening. The macrolide-resistance mutations was in 29% (54/185) of samples from St. Petersburg and in 28% (97/345) from Moscow, with a predominance of the A2059G 23S rRNA genotype of M. genitalium in 57% (31/54) and 68% (66/97), respectively. The second largest proportion were samples in which the nucleotide substitution A→G at position 2058 was detected: 23/54 (42%) from St. Petersburg and 30/97 (30%) from Moscow. Mutations to fluoroquinolones were detected in the parC gene in 47/185 (25%) and 73/345 (21%) samples from St. Petersburg and Moscow, respectively. In Moscow, the amino acid substitution S80I prevails 39% (29/73), the second most abundant D84N occured in 35% (26/73), in St. Petersburg, the similar ratio of S80I was 44% (21/47), D84N was 29% (14/47). In Moscow, seven samples carried the third most common mutation S80N, which is 9%, in St. Petersburg this figure, was twice as high – 19% (9/47). The share of other mutations in the two cities was not more than 1% (D84G, D84Y and S81P). In St. Petersburg, simultaneously with mutations in 23S rRNA, the presence of amino acid substitutions in the ParC QRDR was also confirmed, which represented a case of combined resistance to both groups antibiotics; this level was 1.5 times higher than in Moscow (40% (39/97)) and amounted to 61% (33/54). The most common combination of mutations was A2059G (23S rRNA) + S80I (parC), which made up to 33% (18/54) in St. Petersburg and 25.7% (25/97) in Moscow. The second most abundant genotype – A2058G (23S rRNA) + D84N (parC) accounted for 14% (8/54) and 10% (10/97), respectively. Conclusions. The high level of resistance to macrolides and fluoroquinolones in M. genitalium in two major Russian cities is a significant problem and need for comprehensive resistance testing before prescribing therapy and surveillance programs. The introduction of adapted diagnostic and therapeutic algorithms will reduce the duration of drug administration, increase the effectiveness of therapy and reduce the burden on the health care system. The use of long-term monitoring will improve the policy of antibiotic prescription and reduce the growth of antimicrobial resistance and its spread in Russia.

Objective. To study epidemiology of resistance to macrolides and fluoroquinolones in Mycoplasmagenitalium isolated from pregnant women in Russia and Belarus. Materials and Methods. A total of 107 M. genitalium DNA samples isolated from pregnant women in Russia (n = 58) and Belarus (n = 49) was included in the study. In order to detect markers of macrolide or fluoroquinolone resistance, polymerase chain reaction in real time with the effect of quenching fluorescence of the probe by a primer was used. Confirmation of mutation type was performed using Sanger sequencing. Results. The prevalence of M. genitalium resistance mutations to macrolides in Russia (Tula, Smolensk) and Belarus (Vitebsk, Brest, Minsk) was 3.4% (2/58) and 6.1% (3/49), respectively. Markers of M. genitalium resistance to fluoroquinolones were detected in 5.2% (3/58) of samples from Russia and 8.2% (4/49) of samples from Belarus. The most common mutations associated with macrolide resistance were A2059G (n = 4), fluoroquinolone resistance – D84N (n = 3) and S80I (n = 3) (numbering according to E. coli). Conclusions. The prevalence of M. genitalium resistance markers isolated in pregnant women highlights a need for regular macrolide and fluoroquinolone resistance surveillance.

Objective. To study the prevalence and mechanisms of antibiotic resistance, including carbapenemase production, in clinical isolates of Klebsiella pneumoniae and Escherichia coli isolated in different regions of Russia as part of the sentinel multicenter surveillance study in 2020–2021, and to explore the population structure of K. pneumoniae and the impact of “high-risk clones” on antibiotic resistance. Materials and Methods. Consecutive, non-duplicate isolates of K. pneumoniae (n = 2503) and E. coli (n = 2055) isolated from various specimens (blood, cerebrospinal fluid, respiratory samples, urine, wound secretions, etc.) of hospitalized patients with clinical signs of infection in 55 hospitals of 29 cities of Russia were studied. Species identification of isolates was performed by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF MS). Antibiotic susceptibilities were determined by serial broth microdilution or, in the case of fosfomycin, by agar dilution method, and results were interpreted according to EUCAST v13 MIC breakpoints. Carbapenemase production was determined phenotypically by carbapenem inactivation method (CIM), the presence of genes of the most common serine carbapenemases (KPC, OXA-48) and metallo-β-lactamases (VIM, IMP, NDM) was determined by real-time PCR. K. pneumoniae clinical isolates were genotyped and assigned to the known clonal complexes (CC) and sequence types (ST) using SNP typing and multilocus sequencing typing (MLST) methods. K- and O-serotypes, acquired resistance and virulence genes, and plasmids carrying these genes were characterized using whole-genome sequencing of selected isolates (n = 215). Results. The resistance rates of nosocomial/community-acquired isolates of K. pneumoniae were as follows: amoxicillin-clavulanate – 88.63/57.99%, piperacillin-tazobactam – 82.92/45.49%, cefotaxime – 87.74/56.97%, ceftazidime – 84.76/53.07%, cefepime – 81.43/49.18%, aztreonam – 1.63/53.28%, ceftazidime-avibactam – 30, 88/9.22%, ceftolozan-tazobactam – 70.06/31.35%, ertapenem – 72.10/28.69%, meropenem – 49.60/15.16%, imipenem – 44.54/13.73%, gentamicin – 60.82/30.33%, amikacin – 42.06/17.21%, ciprofloxacin – 85.10/49.39%; trimethoprimsulfamethoxazole – 74.38/48.16%, colistin – 5.96/2.25%. The resistance of nosocomial/outpatient isolates of E. coli were: ampicillin – 84.93/67.67%, amoxicillin-clavulanate – 57.37/39.73%, piperacillin-tazobactam – 19.48/8.70%, cefotaxime – 63.83/34.19%, ceftazidime – 45.32/20.34%, cefepime – 35.95/16.61%, aztreonam – 51.78/26.11%, ceftazidime-avibactam – 5.71/0.80%, ceftolozane-tazobactam – 11, 95/2.22%, ertapenem – 8.18/1.42%, meropenem – 5.17/0.53%, imipenem – 4.95/0.36%, gentamicin – 24.54/13.68%, amikacin – 5.49/1.42%, ciprofloxacin – 54, 14/32.50%, trimethoprim-sulfamethoxazole – 52.21/38.54%, fosfomycin – 2.48/1.43%, colistin – 1.60/1.07%, tigecycline – 6.35/3.11%. The frequency of carbapenemase production among K. pneumoniae nosocomial isolates was 65.32% (OXA-48 – 40.75%, NDM – 30.28%, KPC – 8.74%, OXA-48 + NDM – 10.62%, OXA-48 + KPC – 2.98%, NDM + KPC – 0.45%, OXA-48 + NDM + KPC – 0.20%). More than 70% of nosocomial isolates of K. pneumoniae belonged to only 7 major genetic lineages known as “high-risk international clones”: CC395 – 37.40%, CC23 – 9.59%, CC307 – 8.64%, CC147 – 7.61%, CC15 – 2.95%, CC258 – 2.92%, and CC11 – 2.41%. The population of community-acquired K. pneumoniae was characterized by significantly greater genetic diversity (Simpson diversity index: D = 0.919; 95% CI: 0.904 to 0.933) compared with the population of nosocomial strains (Simpson diversity index: D = 0.815; 95% CI: 0.802 to 0.827). Strains of the “hypervirulent” genetic lineage of K. pneumoniae CC23 were more common in community-acquired infections. Conclusions. The extremely high frequency of resistance to cephalosporins in K. pneumoniae (> 80%) and E. coli (> 60%), as well as the high frequency of combined resistance to aminoglycosides and fluoroquinolones precludes their empirical use for the treatment of serious nosocomial infections caused by these pathogens. K. pneumoniae shows a rapid increase in resistance to carbapenems, mainly due to the spread of carbapenemases of three major groups: OXA-48, NDM and KPC. The overall increase in the frequency of carbapenemase production is accompanied by the growing diversity of carbapenemases, the increasing prevalence of strains producing NDM and KPC enzymes and those co-producing multiple carbapenemases simultaneously. In community-acquired infections, the high prevalence of resistance to cephalosporins in E. coli (> 30%) and K. pneumoniae (> 50%) remains the most important problem.

The paper examines the historical Russian system of measures and its significance for the study of monuments of Russian wooden architecture. The basis of the research is the work of Russian scientists on metrology in construction, as well as research in the field of anthropometry. Continuing the idea of Leonardo da Vinci’s “Vitruvian Man,” this study formulates the hypothesis of a person’s “personal space” in Russian folk architecture. A conclusion is drawn about the modular structure of “spheres of comfort”, which are based on values associated with human growth. “Spheres of Comfort” are used to analyze the plan and facade structures of an archaic peasant building in the Sverdlovsk region. The arrangement of the spheres demonstrates a logical, “organic” composition of the modules. At the same time, numerical analysis of the plan and facade in vershok units is used. Analysis of numerical chains shows that the basis of a series of increasing quantities is the ratio of the diagonal of the square to its side (i.e. √2). The conclusion states the general and specific features of Russian house construction inherent in each specific building. The question is raised about the need for further statistical research and the compilation of regional tables based on anthropometry.

The Gulf of Ob is a unique ecosystem characterized by a complex interaction of river and sea factors. It is exposed to extremely strong seasonal pressure of highly variable temperature, insolation, biogenic load and many other factors, including anthropogenic and technogenic effects. Operational satellite data on the hydrological regime of the Gulf of Ob are crucial for the analysis of different environmental and climate processes as well as assessment of the intensity and scale of anthropogenic and technogenic impacts on the adjacent areas. This study presents an analysis of seasonal and interannual variations of brightness temperature in different parts of the Gulf of Ob in the freezing period based on SMOS (Soil Moisture and Ocean Salinity) data. It was found that in the southern part of the Gulf of Ob, the seasonal and interannual brightness temperature dynamics are similar to freshwater lakes. However, the closer to the Kara Sea the more these dynamics deteriorate and, finally, in the northern part of the Gulf, become similar to those of the central Kara Sea. During the freezing period, changes in the brightness temperature dynamics occur in different parts of the Gulf of Ob. This is explained by an increase in the salinity of water under ice. It was shown that during winter seasons, the zone of fresh and salt waters mixing (the transition zone) can shift far to the south of the Gulf of Ob. Winter shift of the transition zone was compared with climate changes in the region that determine the river runoff and the state of permafrost. The revealed patterns of brightness temperature seasonal variations in different parts of the Gulf of Ob and the associated ice cover phases can be used to assess the hydrological regime in large estuaries of the Arctic in winter, as well as climate changes in the adjacent areas on the basis of satellite microwave radiometry data.

This study aimed to assess the cropping capacity, quality indicators, and phenolic compounds in various tissues of the ‘Kefesiya’ grape variety, influenced by cultivation terroir and agricultural practices. Results indicate that bush training methods, trunk height, and green operations significantly affect cropping capacity, quality, and phenolic content. A multi-armed fan training on a medium trunk in the Sudak terroir yielded 1.8 times higher cropping capacity compared to a one-sided Guyot on a high trunk in Alminskiy. Combining topping with defoliation increased sugar content by 15% and reduced titratable acids by 24% in Sudak, while in Alminskiy, sugar content rose by 5% and acids decreased by 9%. However, this combination also led to a decrease in phenolic compounds across both terroirs. Topping and defoliation positively correlated with phenolic compounds, especially catechins. The study highlights that grape quality and phenolic accumulation are influenced not only by soil and climate but also by specific agricultural practices tailored to crown management.

The primary challenge in red wine production is optimizing conditions for the accumulation of phenolic compounds in grape berries, crucial for the wine’s aroma, color, and flavor. With climate change impacting these components, it’s vital to study the transformation of carbohydrate-acid and phenolic complexes as grapes reach ripeness. This study focuses on the ‘Bastardo Magarachskiy’ grape variety and the effects of foliar mineral fertilization (NTP-Sintez LLC, Belarus) during ripening. Our findings show that experimental processing schemes enhance sugar accumulation in the berries by 1-4 g/100 cm3. The total anthocyanin potential ranged from 1187 to 1684 mg/dm3, with maximum extraction efficiency (61-65%) occurring at sugar concentrations of 21-23 g/100 cm3. Higher sugar levels led to reduced extraction efficiency. The phenolic profile was dominated by flavan-3-ols and anthocyanins, comprising 82.7% to 96.3% of total phenolics. Throughout ripening, there was a significant increase in the mass concentration of primary phenolic compounds in the berries, highlighting the importance of monitoring these changes for optimal wine production.

See the retraction notice E3S Web of Conferences 549, 00001 (2024), https://doi.org/10.1051/e3sconf/202454900001

Objective. To determine spectrum and prevalence of genetic determinants of resistance to macrolides in Mycoplasma genitalium in a Russian patient population. Materials and Methods. A total of 873 M. genitalium-positive samples from five geographical regions of Russia were investigated over the period of 2009–2019 using the previously developed protocol of real-time polymerase chain reaction (allows detecting any nucleotide substitutions in the 23S rRNA gene of M. genitalium at positions 2058, 2059, and 2611). The results were confirmed using Sanger sequencing. Results. The most frequent mutations associated with resistance to macrolides in M. genitalium were the following: A2058G (60.5%) and A2059G (30.2%). The relatively rare mutations were A2058T (7%) and C2611T (2.3%). In the studied period, there was no trend to increase in frequency of mutations associated with resistance to macrolides. The study results are presented as an open project on the AMRcloud platform (https://amrcloud.net/ru/project/demares/). Conclusions. Our data emphasize a need to introduce regular screening of M. genitalium-positive samples for the presence of macrolide resistance-associated mutations into clinical practice.

The building traditions of China go back to the deep past. Wood as a building material helped to withstand many natural disasters, and also provided a high speed of construction. The valleys of the Huang He and Yangtze rivers, where Chinese civilization originated, are rich in vegetation. First of all, it is bamboo, which was widely used for the construction of ancient residential buildings. With the development of building technologies, wood, like bamboo, has become the most common material for building not only residential buildings, but also temples. The basis of Chinese structures is dominated by the frame system, since it is the most resistant to earthquakes and floods. Geographic location also played a role in shaping the structure of the residential building. All constructive solutions of Chinese architecture were preserved by builders for several thousand years. The question arises: are there any modern trends in traditional construction?

Objective. To assess the in vitro activity of ceftazidime-avibactam against clinical Enterobacterales and Pseudomonas aeruginosa isolates in various regions of Russia based on results of local susceptibility testing by disk diffusion method. Materials and Methods. Overall, 160 laboratories located in 61 Russian cities participated in this surveillance during 2018-2020. All consecutive clinical isolates of Enterobacterales and Pseudomonas aeruginosa in each participating laboratory were included in the study. Ceftazidime-avibactam susceptibility testing was done by disc-diffusion method in accordance with current EUCAST recommendations. Susceptibility data for carbapenems and III-IV generation cephalosporins, as well as results of carbapenemases detection, were also reported, if available. All the data were recorded in electronic case report form developed on the OpenClinica online platform (www.openclinica.com). Data analysis and reporting were done using AMRcloud online platform (https://amrcloud.net/). Results. In total, we received information on antimicrobial susceptibility of 22,121 isolates, including 17,456 (78.9%) Enterobacterales and 4,665 (21.1%) P. aeruginosa. Less than 9% of Enterobacterales isolates were resistant to ceftazidime-avibactam. At the same time rates of resistance to ceftazidime, cefotaxime, cefepime, ertapenem, imipenem, and meropenem were 54.1%, 58.9%, 59.4%, 41.4%, 23.9%, and 21.3%. Among Enterobacterales the highest level of resistance to ceftazidime-avibactam was detected in K. pneumoniae (16.5%), lowest – in E. coli (2.1%). Some increase of resistance to ceftazidimeavibactam was noted during the study – from 7.8% in 2018-2019 to 9.6% in 2020 (p = 0.0001). Rate of resistance to ceftazidime-avibactam in P. aeruginosa was 33.1%. At the same time rates of resistance to ceftazidime, cefepime, imipenem, and meropenem were 51.1%, 54.5%, 50%, and 47.3%. During the study there was statistically significant decrease in resistance to ceftazidime-avibactam in P. aeruginosa (p = 0.0001). Resistance rates for all beta-lactams for both Enterobacterales and P. aeruginosa were higher in nosocomial isolates than in community-acquired isolates. Conclusions. Ceftazidime-avibactam demonstrated significantly higher in vitro activity against Enterobacterales and P. aeruginosa Russian clinical isolates comparing with commonly used carbapenems and extended spectrum cephalosporins. Access for all study data available at the AMRcloud online platform (https://amrcloud.net/ru/project/cazavi-1-2/).

In November 2019 the joint group of researcers from the Department of State Protection of Cultural Heritage of the Republic of Bashkortostan and the Institution of History, Language and Literature of Ufa Federal Research Center of Russian Academy of Science has conducted archaeological exploration with the aim to find new monuments of the Middle Ages in the Kushnarenkovsky district of the Republic of Bashkortostan. As a result of this exploration Taganaevo 5 settlement was discovered. Collection of materials found in the course of the test pits drilling consist of animal bones, fragments of pottery and handbuilt ceramics, clay coatening, hand forged nails, a fragment of the iron knife and a lithic core. Taganaevo 5 presents itself a multilayer site. The upper strata of its’ cultural layer refer to the ethnographic time dating back to the 19th century, middle strata contain ceramics of Bahmutino and Kushnarenkovo cultures (V-VII ) and at the lower strata have ceramics of the Srubnaya archaeological culture related to the era of the late Bronze Age and the Eneolithic (Agidel culture). Exploraion works which are aimed at finding new archaeologial sites in the forest-steppe zone of the Cis-Urals as well as the cultural and chronological attribution of these sites contribute greatly to the accomulation of a source base for an archaeological map showing resettlement of the representatives of the Agidel ceramics culture and representatives of the Srubnaya, Kushnarenkovo and Bahmutino ceramics types. As well as the location and spread of the settlements (historical sites, villages and auls) during the Modern Age.

Ekaterina A Skomorokhova,1,2 Tatiana P Sankova,3 Iurii A Orlov,1 Andrew N Savelev,3 Daria N Magazenkova,3 Mikhail G Pliss,4,5 Alexey N Skvortsov,3 Ilya M Sosnin,1 Demid A Kirilenko,1,6 Ivan V Grishchuk,3 Elena I Sakhenberg,7 Elena V Polishchuk,1,8 Pavel N Brunkov,1,6 Alexey E Romanov,1,6 Ludmila V Puchkova,1– 3 Ekaterina Yu Ilyechova1,2 1International Research Center of Functional Materials and Devices of Optoelectronics, ITMO University, St. Petersburg, Russia; 2Department of Molecular Genetics, Research Institute of Experimental Medicine, St. Petersburg, Russia; 3Higher Engineering Physics School of the Institute of Physics, Nanotechnology and Telecommunications, Peter the Great St. Petersburg Polytechnic University, St. Petersburg, Russia; 4Department of Experimental Physiology and Pharmacology, Almazov National Medical Research Centre, St. Petersburg, Russia; 5Laboratory of Blood Circulation Biophysics, Pavlov First Saint Petersburg State Medical University, St. Petersburg, Russia; 6Center of Nanoheterostructures Physics, Ioffe Institute, Russian Academy of Sciences, St. Petersburg, Russia; 7Laboratory of Cell Protection Mechanisms, Institute of Cytology, Russian Academy of Sciences, St. Petersburg, Russia; 8Telethon Institute of Genetics and Medicine, Pozzuoli, Naples, ItalyCorrespondence: Ekaterina Yu Ilyechova Department of Molecular GeneticsResearch Institute of Experimental Medicine, Acad. Pavlov Street, 12, St. Petersburg 197376, RussiaTel +79217605274Fax +78122322307Email ilichevaey@itmo.ruPurpose: The ability of silver nanoparticles (AgNPs) of different sizes to influence copper metabolism in mice is assessed.Materials and Methods: AgNPs with diameters of 10, 20, and 75 nm were fabricated through a chemical reduction of silver nitrate and characterized by UV/Vis spectrometry, transmission and scanning electronic microscopy, and laser diffractometry. To test their bioactivity, Escherichia coli cells, cultured A549 cells, and C57Bl/6 mice were used. The antibacterial activity of AgNPs was determined by inhibition of colony-forming ability, and cytotoxicity was tested using the MTT test (viability, %). Ceruloplasmin (Cp, the major mammalian extracellular copper-containing protein) concentration and enzymatic activity were measured using gel-assay analyses and WB, respectively. In vitro binding of AgNPs with serum proteins was monitored with UV/Vis spectroscopy. Metal concentrations were measured using atomic absorption spectrometry.Results: The smallest AgNPs displayed the largest dose- and time-dependent antibacterial activity. All nanoparticles inhibited the metabolic activity of A549 cells in accordance with dose and time, but no correlation between cytotoxicity and nanoparticle size was found. Nanosilver was not uniformly distributed through the body of mice intraperitoneally treated with low AgNP concentrations. It was predominantly accumulated in liver. There, nanosilver was included in ceruloplasmin, and Ag-ceruloplasmin with low oxidase activity level was formed. Larger nanoparticles more effectively interfered with the copper metabolism of mice. Large AgNPs quickly induced a drop of blood serum oxidase activity to practically zero, but after cancellation of AgNP treatment, the activity was rapidly restored. A major fraction of the nanosilver was excreted in the bile with Cp. Nanosilver was bound by alpha-2-macroglobulin in vitro and in vivo, but silver did not substitute for the copper atoms of Cp in vitro.Conclusion: The data showed that even at low concentrations, AgNPs influence murine copper metabolism in size-dependent manner. This property negatively correlated with the antibacterial activity of AgNPs.Keywords: silver nanoparticles, copper status, ceruloplasmin, alpha-2-macroglobulin, bile, urine

Research background: Swedish economist S.B. Linder considered time as the rarest resource, for the preservation of which people are willing to pay more and more. The problem of monetized evaluation of the benefits of reducing travel time has been relevant for the transport economy since the 1960s. The article is devoted to the study of the specifics of the time resource in modern socioeconomic systems and the analysis of approaches for assessing the benefits of changing time costs. Purpose of the article: To make a retrospective analysis of the phenomenon of time in economics, to investigate the evolution of methodological approaches to the assessment of time resources, to uncover contradictions between the fundamental ideas about the distribution of time resources and methods for assessing the benefits of changes in time costs. Methods: The methods of systematic, retrospective and comparative analysis are used in the paper. Findings & Value added: The conclusions obtained in the course of the study allow us to expand and concretize the methodology of economic assessment of transport projects. There are identified and systematized factors that determine the need for differentiated accounting of changes in the value of reducing travel time for passengers, which ultimately determines the demand for transport services.

This article is devoted to the implementation of a steganographic software tool for hiding infor-mation in MPEG video files. The method of least significant bits was used to hide the data, and error-correcting coding with binary cyclic codes was used to increase the stability of the embedded data. In addition to hiding data, the AES-128 cipher algorithm is applied to the initial data in the algorithm. A software tool has been developed, designed according to the described scheme, which guarantees the im-possibility of detecting and reading the transmitted data by third parties, provided that they do not have access to private data.

Due to global warming, there is a problem to correlate technical and phenolic ripeness in modern viticulture. This study is focused on the use of foliar fertilizers in order to combine the time of beginning of phenolic and technical ripeness. The studies were carried out in 2022 in the conditions of mountain-valley coastal viticulture zone of the Republic of Crimea. Foliar top dressing was carried out with the preparation ‘Agrogemo’ (LLC Pontis, Russian Federation) on wine grape varieties ‘Bastardo Magarachskiy’ and ‘Malbec’. The Glories method was used to estimate the ripeness degree of grapes. The principle of it is to determine the total and extractable anthocyanin potential of grapes. Using of foliar fertilizers made it possible to increase the yield of ‘Bastardo Magarachskiy’ variety by 15.3%, and ‘Malbec’ variety by 18.1%. It was found that the experimental treatment scheme contributed to the later beginning of technical ripeness of grapes, i.e. a low percentage of non-extractable anthocyanins from grape skin compared to the control. Base wines obtained from grapes with the use of organic-mineral fertilizer were characterized by the intense color, balanced aroma and flavor.

Objective. In this study, we examined the prevalence of macrolide-resistant M. genitalium in two Russian cities, Smolensk and Tula, between 2013 and 2017. Materials and Methods. DNA’s samples were isolated from urethral and cervicovaginal swabs using primary screening and tested for macrolide resistance-associated mutations by real-time PCR. This technology makes it possible to identify any nucleotide substitutions in the 23S rRNA M. genitalium gene at positions 2058, 2059, 2611 M. genitalium by melting curve analysis after the amplification. Results. According to the study in two cities (Smolensk and Tula) macrolide resistance-associated mutations were found in 3, 65% of isolates (21/574). The A2058G transition 23S rRNA MGE was the most common mutation that is associated with macrolide resistance: 5/12 (41.6%) – Smolensk, 8/9 (88.8%) – Tula. Rare substitutions have been reported at position A2058T 23S rRNA MGE and at position C2611T23S rRNA MGE. All received data is published at the AntiMicrobial Resistance Map (AMRmap) (http://AMRmap.com). Conclusions. According to our study, the frequency of macrolide-resistance mutations in M. genitalium was not more than 4% in two cities of Central Russia during 2013–2017. Despite the relatively low rates of resistance of M. genitalium to macrolides in Smolensk and Tula, our data emphasize the need for epidemiological surveillance of resistance in this pathogen.

The elastic problem of a spherical core-shell particle with a truncated core is solved within the isotropic classical theory of elasticity. A transition to a Janus particle consisting of two unequal spherical segments with lattice mismatch is demonstrated. Elastic fields in the Janus particle are found and described in detail. The strain energy of the Janus particle in dependence on the interface coordinate is discussed.

This paper presents the study of oxide film heterojunction made of CuAlCrO2 and ZnO:Al (AZO) deposited on fused quartz by sol-gel spin coating. Transmission electronmicroscopy (TEM) analysis has showed that the solvent, which was used to prepare the AZO sol, affects the thickness and planarity of the applied layers and the grain size of the polycrystalline film. The formation of a fine-grained structure of AZO films (grain size is up to 12 nm) leads to the formation of a smoother p-CuAlCrO2 / n-AZO heterojunction. X-ray diffraction (XRD) analysis and TEM have revealed that the heterojunction was formed by AZO polycrystals, which orient axes [101] and [100] against the [101] and [006] directions in CuAlCrO2 polycrystals. The optical transmission of the heterojunction in the visible range reached 70 %. The current -voltage (I-V) characteristics of p-CuAlCrO2 / n-AZO corresponds to a diode one in the range from 4 up to 4 V. For the large values of the reverse bias, an increase in the leakage current is observed, which is associated with tunneling breakdown of heterojunction. The ideality factor of fabricated p-CuAlCrO2 / n-AZO heterojunction is more than 2, which indicates the mechanisms of conductivity by defect-levels. In additional, it has been observed the response of the current-voltage characteristics to the green illumination (532 nm) of the diode that is associated with indirect band-gap absorption in CuAlCrO2. The impact of the white LED illumination had no effect on the current-voltage characteristics of the diode.

Graphene crystals, containing arrays of disclination defects, are modeled and their energies are calculated using molecular dynamics (MD) simulation technique. Two cases are analyzed in details: (i) pseudo-graphenes, which contain the alternating sign disclination ensembles and (ii) graphene with periodic distribution of disclination quadrupoles. Electronic band structures of disclinated graphene crystals are calculated in the framework of density functional theory (DFT) approach. The evolution of the Dirac cone and magnitude of band gap in the band structure reveal a dependence on the density of disclination quadrupoles and alternating sign disclinations. The electronic properties of graphene with disclination ensembles are discussed.

The article is aimed at analyzing and estimating the achievements and threats to the development of vocational education in the context of decentralization of power, defining of directions of improvement of efficiency of administration of vocational education in Ukraine. The status of vocational education development is analyzed, achievements and threats to the development of vocational education are identified. The necessity of decentralization of education administration is substantiated and, on the basis of studying of foreign experience and system analysis of legislative initiatives of the profile ministry, a number of directions of improvement of administration efficiency of vocational education in Ukraine are proposed (introduction of a new model of financing; change of administration system of vocational education; activation of local self-government bodies on administration and financing of vocational education; development of dual form of education and others). Prospect for further research in this direction is to estimate the financial and non-financial benefits of reforming the vocational education for employees, employers, regions, and the State.

Results of the studies of the properties of single-crystalline bulk β-Ga2O3 grown from the melt are presented. High chemical purity and phase uniformity of the grown material are demonstrated. Raman spectroscopy studies confirmed low energies of optical phonons in β-Ga2O3, which makes it a promising material for laser optics applications.

The paper presents the investigation of lithium-potassium-alumoborate glasses (0- 25 mol.% Li2O) doped by copper ions. The effect of glass annealing on the Raman scattering patterns is discussed. It is shown that such an annealing results in drastic changes in the Raman scattering patterns for the glass with 10 mol.%of Li2O: the band at 481 cm-1 conventionally attributed to boron-oxygen bonds in metaborate rings disappears, while a set of narrow bands at 1236, 1500, and 1783 cm-1 is observed. We consider one of these bands, 1783 cm-1, as being the identifier of the formed Li(Al7B4O17) nanophase, XRD analysis supported the above suggestion. The effect of newly formed nanophase on the luminescence spectra is discussed.

The article presents the results of research on the peculiarities of proofing and baking bakery products using a steam-convection oven for continuous proofing and baking. The aim of the work was to develop recommendations for setting parameters for production of bakery products in the steam-convection oven at continuous proofing of dough pieces and bakery products. For control samples baking the author used a proofing box for final proofing and a convection oven. The analysis of the structural and mechanical properties of crumb was made using device «Strukturometer-1», and loss of weight after baking was measured using standard methods. Intensity and color uniformity of crust were determined applying an ingenious method using flatbed scanner. Automatic recorder monitored the temperature in the baking chamber and inside the baked dough pieces. To determine the changes in product volume and proportion of the formed crumb during baking process, an original optical method was used. As a result of the research the author determined that there is a dependence of the dynamics of baked dough pieces quality indicators on proofing and baking parameters. It is shown that the main differences are observed during the first baking period. The detected regularities are associated with smooth heating of the steam-convection oven baking chamber and high relative humidity of the medium at the beginning of baking process. When using a steam-convection oven, the crusts had uneven color which is obviously due to condensation of a large amount of moisture on the surface during the initial baking period and, consequently, different rates of heating of individual parts of the crust. By the moment when baking process was over control samples and developed samples had insufficient differences concerning structural, mechanical and physicochemical parameters. It was determined that in case of continuous proofing and baking in steam-convection oven it is necessary to extend the baking process and increase dough humidity compared to traditional baking method.

A screw dislocation perpendicular to free surfaces and an interface in a two-phase plate, and a screw dislocation piercing a two-phase hollow sphere are considered. The analytical solutions of the boundary-value problems have been found for the first time with the help of the virtual defects technique. Elastic fields of the screw dislocation in the plate are presented in the form of integrals with Bessel functions. Elastic fields of the screw dislocation in the hollow sphere have the form of series with Legendre polynomials. Stress distributions in both of the considered geometries are plotted. The influence of the geometric parameters of the considered solids and the ratio of the shear moduli on the stresses is analyzed. The interaction of a screw dislocation with a parallel edge dislocation is discussed.

The formation of copper pentagonal micropyramids (PMPs) with high (multiatomic) spiral growth steps, which are grown by electrocrystallization with mechanical activation of the cathode, is studied experimentally. A new spiral-layer growth mechanism for the formation of such PMP is proposed. It is shown that PMPs grow on flat pentagonal microcrystals (PMCs) formed initially and containing fivefold twins with one of the twin boundaries being inclined by the angle of 35°16’ to the {110}-type substrate crystallographic plane. Such crystal geometry causes an inclined growth step on the PMC surface. The preferential deposition of metal atoms on this step leads to the spiral-layer PMC growth and the formation of PMPs with a structure inherited from the PMCs.

This work presents characteristics of qualitative and quantitative composition of phenolic compounds and their changes during long-term storage of ‘Italia’ table grapevine cultivar. The studies were carried out using method of HPLC on a Shimadzu LC 20 Prominence chromatograph with diode-array detector of ultraviolet and visible range. Grapes were sampled fresh and after long-term storage (60 days). Three groups of phenolic compounds were identified: hydroxycinnamic acids, procyanidins, and flavonols. It is established that major components of phenolic complex of ‘Italia’ grapes are procyanidins, specifically d-catechin and epicatechin, amounting 33% and 34%, respectively, and also procyanidin B2 with high proportion of 19%. At the end of long-term storage, a decrease in the mass concentration of phenolic compounds was revealed, the content of hydroxycinnamic acids decreased by 62,3%, procyanidins by 50,1%, and flavonols by 51,0%. A decrease in the activity of polyphenoloxidase enzyme to 60th day of storage by 8,53%, relative to the control, was established. In the process of analysis of variance, statistically significant differences in the components of phenolic profile in grapes of ‘Italia’ cultivar between the beginning and end of storage were established using Student's t-test, which varied from 4,43*10-15 to 6,07*10-7, being so significantly less than 0,05.

We consider the dynamic properties of a system of three differential equations known as the oreganator model. This model depends on four external parameters and describes one of the periodic Belousov–Zhabotinsky reactions. We obtain broad conditions for the parameters that ensure the existence of nonstationary steady-state regimes in oregonator model. With classical values of the parameters, the localization of the limit (at a long time) dynamics in the phase space has been improved. In fact, using numerical analysis, we significantly narrow the bounded region of the phase space containing the trajectories of the system. An iterative procedure is proposed for the approximate localization of closed trajectories (cycles) of the system on algebraic surfaces in R3. A promising problem of theoretical substantiation of the numerical convergence of this procedure is posed.

In this work the effect of post-harvesting aerosol spraying with calcium-containing preparations Master Green Ca (1.75 g/l) and Brentax KCa (2.5 g/l) on changes in the indicators of commercial quality and natural weight loss of grape cv. Shokoladniy in the dynamics of long-term storage was studied. The studies were carried out in 2019-2020. Experimental samples were collected on the 30th and 60th day of storage. It has been established that aerosol spraying contributed to a decrease in the natural loss of bunch weight by 5.7- 6.5%. The amount of total sugars in experimental samples decreased to the 60th day of storage by 15.3% and 10.2% compared to the control. The preparations positively impacted organoleptic indicators during the storage: the tasting assessment of experimental batches of grapes to the end of storage exceeded the control by 6.7-10.3%.

An increase in ambient temperature affects the quality of wines, which is largely due to a change in the metabolism of phenolic substances in grapes. A possible solution to the problem is the use of autochthonous varieties adapted to unfavorable conditions. The influence of agroecological conditions of 4 vineyards on the phenolic complex of the Crimean grape variety ‘Kokur Belyi’ has been studied. Methods of geoinformation and mathematical modeling, HPLC were used. It is established: vineyards differ in heat resources in the row: Vilino < Privetnoe < Solnechnaya Dolina < Morskoe. An increase in the heat supply of territories caused the accumulation of phenolic acids, flavonols, (+)-D-catechine and procyanidins B4 in grape seeds and a decrease in the content of phenolic acids, (-)-epicatechine, procyanidins B3 in skin and pulp. The highest content of procyanidins in seeds and the lowest content of monomeric phenolic components in skin and pulp was determined in grapes from the village of Morskoye. Grapes from Vilino contain 3.7 times less procyanidins in the seeds, 6.5 and 3.6 times more monomeric components in the skin and pulp. Conclusion: the heat supply of vineyards contributes to both the biosynthesis of monomeric phenolic components and their polymerization during the ripening of grapes, leads to a significant differentiation of the phenolic complex of grapes ‘Kokur Belyi’.

Mycoplasma pneumoniae is a bacterial pathogen cause of upper and lower respiratory tract infections, transmitted by airborne droplets, causing outbreaks of pneumonia mainly in closed groups. According with recommendations, M. pneumoniae infections can usually be effectively treated with macrolides, which are generally considered the first-choice antibiotics in young adults. However, macrolide resistance has been observed in a number of countries. Macrolide resistance phenotypes are defined by specific point mutations in the V domain of the single-copy 23S rRNA gene of M. pneumoniae, mainly at positions 2063, 2064 and 2617 (numbering according to M. pneumoniae). Identification of appropriate single nucleotide substitutions allows effectively predicting the phenotype of resistance to macrolides, but the methods used for this purpose currently are laborious and costly. The present study is devoted to the development and validation of a new method for the determination of mutations associated with macrolide resistance in M. pneumoniae, as well as its use for the analysis of clinical specimens obtained from 31 patients with pneumonia treated in a military hospital. Two and one patients had M. pneumoniae isolates with a substitution at positions 2063 and 2064, respectively. In one case, a mixed population of wild-type and mutated M. pneumoniae isolate was observed. A rare mutation variant in the 23S rRNA gene of M. pneumoniae corresponding to the genotype C2617G was found in one patient. Our PCR-RT assay is able to discriminate between wild-type and resistant genotypes of M. pneumoniae directly from clinical specimens can be used to quickly identify type of mutations and predict possible resistance respiratory mycoplasmas to macrolide antibiotics. This assay will allow clinicians to shorten the time to the initiation of effective disease treatment.

The study included 418 of the 841 clinical isolates of S. aureus, which were regarded as nosocomial. These 418 clinical nosocomial S. aureus strains were isolated in 2013-2014 from patients hospitalized in 25 hospitals in 19 Russian cities. Methicillin-resistance rate was 24.9% (n=104). Antimicrobials with the highest activity with no resistance detected were vancomycin, linezolid, cefilavancin, telavancin, daptomycine, tigecycline and fusidic acid. Other highly in-vitro active antimicrobials were trimethoprim-sulfamethoxazole, mupirocin and ceftaroline (0.5%, 2.2% and 4.3% non-susceptible isolates respectively). MRSA isolates were substantially less susceptible compare with MSSA to gentamicin (1.3% vs. 83.7%), clindamycin (1.6% vs. 40.4%), rifampicin (2.2% vs. 27.9%), tetracycline (12.1% vs. 35.6%), chloramphenicol (9.9% vs. 71.2%), ceftaroline (0% vs. 17.3%), ciprofloxacin (6.1% vs. 89.4%), and erythromycin (18.5% vs. 58.7%).

We investigate electron spin resonance (ESR), absorption, and photoluminescence spectra of potassium-boron copper-containing glasses. In previous works it was found, that the considered glasses demonstrate a red shift of photoluminescence. In present work, we provide an evidence on the formation in glasses specific copper-containing structures that can be responsible for the red shift of photoluminescence “copper” band.

Iurii A Orlov,1,2 Tatiana P Sankova,1,2 Polina S Babich,3 Ilya M Sosnin,1,4 Ekaterina Yu Ilyechova,1,5 Demid A Kirilenko,1,6 Pavel N Brunkov,1,6 Gennadii L Ataev,3 Alexey E Romanov,1,4,6 Ludmila V Puchkova1,2,5 1International Research and Education Center “Functional materials and devices of optoelectronics and microelectronics”, ITMO University, 2Department of Biophysics, Peter the Great St Petersburg Polytechnic University, 3Department of Zoology, Herzen State Pedagogical University of Russia, St Petersburg, 4Nanocatalysts and Functional Materials Laboratory, Togliatti State University, Togliatti, 5Department of Molecular Genetics, Research Institute of Experimental Medicine, 6Ioffe Institute, St Petersburg, Russia Abstract: Silver nanoparticles (SNPs) are new functional materials that are widely used in biomedical and industrial technologies. Two main features that make SNPs valuable are their strong antibacterial effects and low toxicity to eukaryotes. In this study, SNPs were synthesized using a modified method of reducing the metal ions to their atomic state followed by crystallization. SNPs were characterized by UV/vis spectroscopy, X-ray diffractometry, atomic force microscopy, and transmission electron microscopy (TEM). The SNPs were spherically shaped with an average linear dimension of 20 nm. In aqueous solution, the SNPs were beige-yellow in color, and they formed a black color in bacteria-rich growth media. The toxicity and bioavailability of the SNPs were tested using Escherichia coli cells and C57Bl/6 mice. Although the SNPs displayed bactericidal activity, an E. coli cell strain transformed with an expression plasmid carrying a human CTR1 ectodomain with three motives that bind Cu(II), Cu(I), and Ag(I) demonstrated increased resistance to treatment with SNPs. TEM showed that the SNPs were absorbed by the E. coli cell, and flow cytometry showed that the SNPs induced apoptosis-like death. In mice treated with SNPs (daily intraperitoneal injection of 10 µg SNPs/g body weight over 4 days), the ceruloplasmin (Cp) oxidase activity in the blood serum decreased. However, level of Cp gene expression, the relative contents of the Cp protein in the Golgi complex and in the serum did not change. Treatment with SNPs did not influence the activity of superoxide dismutase 1 in the liver and had no apparent toxic effects in mice. These findings expand the scope of application for the use of new SNPs. The data are discussed in a paradigm, in which the effects of SNPs are caused by the interference of silver ions with copper metabolism. Keywords: silver nanoparticles, bioavailability, apoptosis-like death, mammalian copper metabolism, copper status

The article attempts to present a holistic view of the theory, practice and history of the development of public education in the modern Volga Federal District in the chronological framework of the second half of 19-20th centuries. In order to achieve the presented goal of the research, we used theoretical methods (analysis, synthesis, generalization, modeling, abstraction, classification, systematization, periodization) and historical and pedagogical methods of research, namely, comparison and contrast (comparativist), historical and structural and historical and typological. Within the geographical framework of the Volga Federal District as a complex sociocultural region with the cohabitation and interaction of different nationalities and confessions, the history of education in a holistic form has not been considered before. The authors proposed modern scientific and pedagogical methodological approaches to the analysis of the history of education of the region represented: pedagogical axiology, pedagogical regionalism, pedagogical comparativism, pedagogical synergetic, systemic-activity approaches. For the first time, the theory and practice of public education in the modern Volga Federal District are considered in the context of a unified historical and pedagogical process (without the traditional division into pre-revolutionary and Soviet stages). In addition to theoretical results of the historical and pedagogical research and practical: the formation of a bank of archival materials on the history of education in the republics and regions of the Volga Federal District; publication of the textbook “History of Public Education in the Volga Federal District”; publications in peer-reviewed journals devoted to the study of educational development in the Volga Federal District; organization and conduct of the Pedagogical Festival of Student Science of the Volga Federal District republics; as well as the preparation and production of a series of television programs about the history of the development of public education in the republics and regions of the Volga Federal District.

The study object - technological process of interference fits assembling is identified, the scheme with oil supply from the end face is adopted as the technological scheme. Technological pressing equipment is considered, its applicability for hydropress assembling is evaluated. The main aspects of hydropress joints assembling on a base of mechatronic press are established: increasing of assembling performance, its stability. Mathematical models and an algorithm that regulate the process of liquid friction and formation of an oil layer between the contacting surfaces of joint mating parts are developed. The basic requirements for the regulation channels of control system that ensure the quality of hydropress fits during automated assembling are established.

Research background: The problem of evaluating the results of innovative transport technologies is extremely important for their implementation and realization. The assessment of possible benefits of innovative transport projects is complicated by a number of circumstances. These include a wide range of arising effects in various forms and the long life cycle of the project, which increases the difficulty of reliably measuring the various costs and results. Thus, it can be argued that outdated approaches and methods for assessing the new transport products hinder the development of innovations. Purpose of the article: to identify directions for assessing the travel time value in the analysis of the transportation projects economic efficiency. Methods: The study was conducted by means of scientific methods such as the comparative analysis, system method, generalization and abstraction. Also, we use the results of expert assessments based on depth-in interviews. Findings & Value added: The article substantiates the necessity of taking into account the incremental benefits for passengers from travel time reduction as the most important aspect of transport projects implementation, ensuring the formation of the whole range of external social and economic effects; The multi-factor dependence of the travel time value for a passenger, which is characterised by its heterogeneity during a journey, has been substantiated. The necessity of assessing changes of travel time values in the general transport costs of passengers has been substantiated, taking into account the several factors that lead to different time values. A visual model of the socio-economic effects formation of the HSR project, caused by changes in the passengers’ total transport costs and increased transport accessibility of the region, is proposed.

The article reviews the history of establishing Moscow University and the first decade of its performance in the context of considering the European experience and the role of visiting foreign professors. Attention is drawn to the use of the experience of European universities in the projected management system of Moscow University and ensuring its autonomy by M.V. Lomonosov. The authors show the role of the I.I. Shuvalov, the favorite of Empress Elizabeth Petrovna, in implementation of the project of Moscow University, and analyze the developed hierarchy of the university’s governing bodies, the structure of administrative positions, and the system of privileges granted by the government. The dominant role of visiting professors in establishing and functioning of the university’s faculties is revealed in detail, as well as the conditions created for their work in Moscow, and the personal role of each of the invited professors in the educational process during the first years of Moscow University. The authors reveal the conflicts between Russian and foreign professors and show their inevitability at the stage of establishing Moscow University, drawing attention to the transition of the educational process to the Russian language as the teaching language during the first years of the reign of Catherine the Great, Empress of Russia.

This research unveils the nature of relations among such phenomena as "ethnos", "culture" and "language". Vested with the function of axiological retranslation, mottoes of the U.S. Armed Forces services and branches make essential part of the military lingo as a semi-autonomous existential form of the national language. Clichéd formulas of the professional sublanguage official register explicitly reflect collective mindset, dominant values, behavioral patterns, and conceptosphere of America’s society military cluster. The authors arrive at the conclusion that military mottoes are typical of vocativity, brevity, metaphoricity, widespread use of Latinisms, stereotypogenicity, and appeal for professional ethos.

One of the main factors increasing the productivity of vineyards and the quality of fresh grapes during storage is the use of vegetative treatments with bioregulators of various physiological effects. This work is devoted to the study and comparative assessment of various foliar treatments with the growth regulator "Albit", "Glycerol" and a complex of fertilizers LLC "Biochefarm Rus" on agro biological and commercial quality characteristics of the table grape variety ‘Moldova’ during the longterm storage. Foliar treatments were carried out in the main phases of the growing season: 'before flowering', 'after flowering', 'the beginning of growth', 'the beginning of ripening'. Sampling for analyzes was carried out for 30, 90 and 150 days of storage. It was found that foliar treatment leading to a significant increase in cropping capacity of grapes by 26.8% ("Biochefarm"), 36.5% ("Albit") and 20.0% ("Glycerol"). The increase in the average weight of the bunch in the experimental variants grew by 29.6-31.3%. The use of experimental schemes of foliar treatments made it possible to increase the keeping quality of table grapes: the weight loss of bunch decreased by 0.3-3.5%. The tasting assessment of the experimental batches of grapes at the end of storage exceeded the control by 5.6-6.9%.

In the transition to automated and automatic manufacturing an urgent problem is to increase the reliability of mobile robots (MR) and their drives, creation of devices to monitor the technical characteristics of MR, diagnose and predict the remaining resource. Inspite of the high relevance of the diagnosing MR drives problem, there are no generally accepted methodology for diagnosing MR drives, criteria for selecting methods, parameters and volumes of diagnostics at present. An unsolved problem, related to the diagnosis of MR drives and the prediction of their residual life remains, is the development of methods that allow to carry out of automatic complex multiparametric diagnostics and prediction of the residual life using artificial intelligence methods. Effective fault detection and diagnosis can improve the reliability of the MR drive and avoid costly maintenance. In this paper a fault detection scheme for synchronous motors with permanent magnets based on a fuzzy system is proposed. The sequence current components (positive and negative sequence currents) are used as fault indicators and are set as input to the fuzzy fault detector. The expediency of the proposed scheme for determining of various types of faults for a synchronous motor with permanent magnets under various operating conditions is simulated using the SimInTech software.

The article presents the results of studies on the use of calcium-based bioactivators in aerosol spraying of V. vinifera L cv. Red Globe in the postharvest period and the assessment of its impact on the quality of grapes during storage (30 and 90 days). In the international practice calcium-containing preparations are widely used to solve problems of quality preservation of the resulting crops. Level of calcium in fruits and berries affects the duration of storage by increasing the resistance to pathogens. Calcium-containing preparations are safe for human health and can become good alternative to synthetic fungicides and SO2 when storing and improving the quality of table grapes. In the work we used calcium-based bioactivators Brentax Ca in concentrations of 50 and 75g per 20l of water; Brentax KCa - in concentration of working mixture - 50g / 20l and Master Green Ca in concentration of 35g / 20l. The following parameters were evaluated: mass concentration of dry matter and titratable acids, weight loss of bunch and organoleptic parameters.High efficiency was performed by preparations Master Green Ca and Brentax Ca 50. Their use made it possible to reduce significantly the weight loss of bunch towards the end of storage by 45 and 34% compared to the control, to preserve organoleptic parameters (7.8 and 7.6 points), respectively, at the level of fresh grapes during storage owing to safekeeping of stem in a flexible condition, preservation of natural color, turgor of berries and balanced flavor. The data obtained will allow improve the system of long-term storage of grapes due to the use of calcium-based bioactivators Master Green Ca and Brentax Ca in concentration 50g / 20l of water.

The article draws attention to the sphere of historical and pedagogical knowledge. Being a product of the development of human society, it lies at the core of contemporary and future pedagogical culture. The mature conventional wisdom helps people to forge their own path in the modern world, to determine their self-identification and the prospects for their personal growth. The historical heritage of the first half of the 20th century, rich in invariant ideas, scientific and pedagogical experience, is viewed here by means of its inclusion in the construction of all spheres of life in modern Russia and Kazakhstan, including the development of pedagogy and psychology. The article reveals the stages of scientific activity of the outstanding teacher and psychologist A.P. Nechaev, characterizing the main milestones and dynamics of the formation of domestic experimental pedagogy at the beginning of the 20th century. It highlights the scholar’s ideas about syndromic psychology, holistic, mental and moral development of the personality, which are relevant even today. The research is based on conceptual ideas of dialogical pedagogy, problem-personalistic approach, retrospective, comparative historical and historical-phenomenological methods. Experimental pedagogy as a phenomenon of the early 20th century ceased to exist without quite exhausting its capabilities, but it laid the foundation for and determined the strategic development direction of child psychology, pedagogical psychology, genetic psychology, childhood ethnography, differential psychology and differential psychophysiology, and the experiment became a sturdy part of the scholarly apparatus of pedagogy and psychology. The potential of experimental pedagogy made it possible to pose and solve large-scale issues of creating a new school, scientific and pedagogical centers, finding solutions to a wide range of psychological and pedagogical problems, designing research schools that develop natural science, psychological and pedagogical problems of education and upbringing. The invariant ideas of this direction of moral, mental and intellectual development of students are also relevant at the beginning of the 21st century and can be used in the practice of contemporary school education.

Determination of frictional condition of the running surface and side surface of the top of rail (lubrication) that ensures the best interaction of the rolling stock wheels and the rail, reduces the force action and thus ensures the track stability and reduced side wear of rails in the curved tracks is relevant for all the rail net.The objective of research is to determine the influence of frictional condition of the track rail surfaces on the interaction forces in the “wheel/rail” contact with various motion parameters (speed, radius).The theoretical and experimental methods were used in the research. The theoretical methods include multioptional computer modelling of axial and lateral forces that appear in the curved tracks during the freight train movement in the software package “Universal Mechanism”. The modelling results were processed with the use of correlation and regression analysis. The experimental methods include full-scale measurements in the existing track and results processing.According to the research results, the theoretical algorithms for assessment of influence of the running surface lubrication on the forces. The option of frictional condition of the wheel and rail interaction surfaces has been established to ensure reduction in the operating expenses for surfacing and rail replacement, energy costs for haulage of freight train.

Telavancin is a first marketed representative of semisinthetic lipoglycopeptides — new generation of glycopeptide antimicrobials. Telavancin is registered for the treatment of nosocomial pneumonia and skin and soft tissue infections caused by Gram(+) microorganisms, including multiresistant strains. Another perspective area of telavancin use — bloodstream infections, such as bacteremia and endocarditis. Telavancin is in vitro active against broad range of Gram(+) aerobic and anaerobic bacteria, including strains resistant to other antimicrobials: MRSA, MRSE, VISA, daptomycin non-susceptible strains of S. aureus, penicillin-resistant S. pneumoniae, ampicillin-resistant enterococci and enterococci with VanB phenotype of resistance to vancomycin. Talavancin is rapidly bactericidal due to its dual mechanism of action; it is active against intracellular bacterial cells and biofilmforming cells. Article reviews data of current in vitro and in vivo studies of telavancin, as well as clinical trials results.

Digitalization and automation in all sectors of the economy have increased the efficiency and effectiveness of various systems and processes. Online technologies are also widely used in the higher education system. Online learning, e-learning, e-learning tools and digital assessments are not innovations in the full sense of the word, but the widespread use of these technologies took place in mid-2020, due to the COVID-19 pandemic. The using the online format of training by universities and schools in the spring of 2020 was mostly spontaneous and forced, but later, due to the complication of the sanitary and epidemiological situation in the world, as well as in order to improve electronic pedagogical technologies in the field of certification of university students, this technology became the subject of consideration by specialists from different industries and, above all, from the field of pedagogy. The authors made attempts to study the main problems of University students passing exams and tests in an online format in order to avoid them in the future, and also developed ways to improve this technology.

An ATOVS and MERRA data archive containing temperature values at isobaric levels from 1000 to 50 hPa was formed to study the atmospheric effects observed over the foci of two strong earthquakes with magnitudes M = 7,3. The first seismic event was recorded on August 19, 1992 in the northern Tien Shan and the second on November 12, 2017 in the border areas of the Zagros folded mountain belt. According to USGS intense aftershock activity in these regions began with these seismic events. Using correlation and spectral analysis we studied the vertical profiles of temperature and pressure in a disturbed atmosphere over seismically active regions. The modified anomaly index was calculated at isobaric levels from 500 to 50 hPa and on its basis the registered temperature anomalies were investigated.

We present a complete analytical solution for the plane elasticity problem of a concentrated force acting on a half-space weakened by a circular hole. The solution is based on the algorithm proposed by G.B. Jeffery in 1920 for elasticity problems, using bipolar coordinates to characterize the geometry. We found the biharmonic stress function describing stresses and strains in the half-space and the associated biharmonic function that allows determining the displacement field. Both functions are given in the form of Fourier series with quite compact coefficients. Particular attention is paid to the displacements at the plane boundary of the half-space. It is discussed how these displacements can be used in an inverse procedure for the identification of a circular hole diameter and position.

This paper deals with the study of capabilities for strengthening an aluminum alloy with hollow ceramic microspheres. The results of the microstructural analysis and the investigation of strength properties for the aluminum-silicon alloying system are described herein. For comparison Experiments have been carried out for comparison both with classical microspheres and with chromium- and chromium-carbide-coated microspheres to increase the melt adhesion to a microsphere