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Purposes To evaluate and compare the effects of occlusal hypofunction on root surface areas (RSAs) of permanent teeth in patients with Class III and Class I skeletal relationships.Materials and Methods Thirty-two CBCT images of all permanent maxillary and mandibular teeth, except the third molars, from 16 patients (mean age 23.71 ± 4.04 years) exhibiting Class III skeletal relationships and 16 patients (mean age 24.53 ± 4.91 years) exhibiting Class I skeletal relationships were imported into the Mimics software version 15.01. The outer boundaries of the teeth in two-dimensional images were manually identified on each CBCT slice along the coronal, sagittal, and axial planes to construct three-dimensional models. The tooth models were transferred into 3-matic version 7.01 to specify cementoenamel junctions and calculate RSAs. Intra-examiner reliability was assessed using the intra-class correlation coefficient. The RSAs of each tooth type from the two groups were illustrated and compared using independent t-tests (p

In an effort to mitigate the environmental impact of chemical fertilizers, plant growth-promoting rhizobacteria (PGPR) have emerged as a more sustainable alternative. Streptomyces saraceticus 31 (‘SS31’), a new strain of biocontrol bacteria, was inoculated into rhizosphere soils of ‘Benifuji’ grape to evaluate its impact on grape roots and berries. The results indicated significant improvements in soil fertility, with higher levels of organic matter, phosphorus, potassium, and nitrate nitrogen compared with those of the controls. Moreover, ‘SS31’ application elicited a notable reduction in soil pH levels, along with a substantial augmentation in the enzymatic activities of both phosphatase and invertase. The grapes treated with ‘SS31’ exhibited a notable increase in the number, length, surface area, and volume of fine roots in both 0- to 10-cm and 10- to 20-cm soil profiles. The application of ‘SS31’ resulted in the observation of greater diameter, lower density, and larger lumen area, along with increased specific hydraulic conductivity in the vessels of roots with 1- to 2-mm diameters. Despite a slight reduction in berry weight compared with that of the controls, ‘Benifuji’ grape berries displayed higher total soluble solids and lower total titratable acidity after ‘SS31’ application. Furthermore, ‘SS31’ treatment elevated the levels of volatile compounds in berries, especially fatty acid-derived compounds. A network analysis revealed a robust positive correlation between the observed improvements in grape berry quality and the morphology as well as the hydraulic conductivity of the grape fine roots. In conclusion, these findings suggest that ‘SS31’ has the potential to enhance grape root function by expanding the root absorption area and facilitating water transportation. This, in turn, may improve the flavor and aroma of ‘Benifuji’ grape berries.

The growth of root system directly affects the absorption and utilization of soil water and nitrogen, and understanding the responses of root characteristics to water and nitrogen regimes is thus crucial for optimizing water and nitrogen management. The root characteristics of each soil layer, i.e., root length, root surface area, and root volume, as well as fruit yield and water use efficiency of greenhouse tomato under drip fertigation in response to different irrigation levels and nitrogen rates were explored in northwest China. There were four irrigation levels, i.e., 50% ETC (W1), 75% ETC (W2), 100% ETC (W3), and 125% ETC (W4), where ETC is the crop evapotranspiration, and four nitrogen rates, i.e., 0 kg ha−1 (N1), 150 kg ha−1 (N2), 250 kg ha−1 (N3), and 350 kg ha−1 (N4). The results showed that reasonable irrigation and nitrogen regimes (W3N3) significantly increased fruit yield by 31.64% and root length, root surface area, and root volume by 45.03%, 61.24%, and 148.21% compare to W3N1, respectively. The promoting effect of increasing irrigation level on root characteristics increased with soil depth and had the greatest increases in root volume by 27.07%, 123.43%, and 211.47% for the 0–10, 10–20, and 20–30 cm soil layers, respectively. In addition, reducing irrigation level significantly increased the percentages of roots in the top soil by 29.71%, 26.77%, and 18.53% for root length, root surface area, and root volume, respectively. The reasonable nitrogen rate (N3) significantly increased fruit yield by 41.11%, water use efficiency by 34.42%, and root length, root surface area, and root volume by 40.42%, 41.44%, and 112.76%, respectively. The over-application of nitrogen (N4) reduced root characteristics of all soil layers, fruit yield, and water use efficiency. The promoting effect of increasing nitrogen rate on root length of each soil layer decreased with soil depth, by 71.01%, 48.96%, and 15.71% for 0–10, 10–20, and 20–30 cm soil layers, respectively. Irrigation level was the main factor dominating the root growth of each soil layer. The correlation analysis showed that fruit yield had significantly positive correlations with root characteristics in all soil layers, while water use efficiency had significantly positive correlations with the percentages of root length and root surface area in the 0–10 cm soil layer. In conclusion, rational water and nitrogen regimes achieved better fruit yield by promoting root growth of greenhouse tomato, and the water use efficiency of greenhouse tomato was improved by increasing the root percentage in the topsoil layer to alleviate the adverse effects under water stress conditions. This study reveals how irrigation volume and nitrogen application can enhance tomato yield and water use efficiency by regulating root characteristics and vertical root distribution, providing support for understanding the response of root systems to changes in soil water and nitrogen conditions.

Abstract Objective Accurate quantification of the root surface area (RSA) plays a decisive role in the advancement of periodontal, orthodontic, and restorative treatment modalities. In this study, we aimed to develop a dynamic threshold-based computer-aided system for segmentation and calculation of the RSA of isolated teeth on cone-beam computed tomography (CBCT) and to assess the accuracy of the measured data. Method We selected 24 teeth to be extracted, including single-rooted and multi-rooted teeth, from 22 patients who required tooth extraction. In the experimental group, we scanned 24 isolated teeth using CBCT with a voxel size of 0.3 mm. We designed a computer-aided system based on a personalized dynamic threshold algorithm to automatically segment the roots of 24 isolated teeth in CBCT images and calculate the RSA. In the control group, we employed digital intraoral scanner devices to perform optical scanning on 24 isolated teeth and subsequently manually segmented the roots using 3-matic software to calculate the RSA. We used the paired t-test (P 0.05). As per the Bland-Altman plot, the results were evenly distributed within the region of ± 1.96 standard deviations of the mean, with no increasing or decreasing trends and good consistency. Conclusion In this study, we designed a computer-aided root segmentation system based on a personalized dynamic threshold algorithm to automatically segment the roots of isolated teeth in CBCT images with a voxel size of 0.3 mm. We found that the RSA calculated using this approach was highly accurate, and a voxel of 0.3 mm in size could accurately display the surface area data in CBCT images. Overall, our findings in this study provide a foundation for future work on accurate automatic segmentation of tooth roots in full-mouth CBCT images and the computation of RSA.

A hydroponic trial was conducted to study the effect of chloride salinity in simulated effluent on Cd accumulation by tobacco. Leaf surface area (LSA) and root surface area (RSA) measurements were incorporated as possible determinants of Cd uptake rate by plants. Results showed that individual plant differences in Cd content were normalized when including RSA to express Cd uptake rates by plants but not including LSA. A biotic ligand model (BLM) fitted to predict Cd uptake, estimated active and almost linear uptake of the free Cd2+ ion by tobacco plants, while virtually no changes in the chloride complex (CdCl+) uptake were predicted, presumably due to a rapid saturation of the hypothetical root sorption sites at the concentrations used in this trial. Nicotiana tabacum var. K326 is evidenced to be a species potentially suitable for biological wastewater treatment using rhizofiltration at concentrations commonly found in salt-affected wastewater, with high Cd accumulation (185 to 280 mg/kgd.m.) regardless of water salinity and tolerance up to 80 mmol/L NaCl.

Silicon (Si) is absorbed and accumulated by some plant species; it has been shown to improve plant growth and performance. The beneficial role of Si in plants is based on the fundamental assumptions, and the biological function of Si is still being researched due to its complex nature, distinctiveness, and interaction. The present study included two distinct experiment sets: a screening test and an advanced test. In the initial examination, we used 21 soybean (Glycine max L.) cultivars. Following the evaluation, we chose four cultivars to investigate further. In particular, the positive response cultivars, Taeseon and Geomjeongsaeol, showed a 14% increase in net photosynthesis (PN), and a 19–26% increase in transpiration in Si-treated plants when compared to the control plants. Si-treated Taeseon, Geomjeongsaeol, and Somyongkong, Mallikong cultivars showed significant differences in root morphological traits (RMTs) and root system architecture (RSA) when compared to the control plants. Taeseon and Geomjeongsaeol showed a 26 and 46% increase in total root length (TRL) after Si application, respectively, compared to the control, whereas Mallikong and Somyongkong showed 26 and 20% decrease in TRL after Si treatment, respectively, compared to the control. The Si application enhanced the overall RMTs and RSA traits in Taeseon and Geomjeongsaeol; however, the other two cultivars, Somyongkong and Mallikong, showed a decrease in such RMTs and RATs. Furthermore, to understand the underlying molecular mechanism and the response of various cultivars, we measured the Si content and analyzed the gene expression of genes involved in auxin transport and root formation and development. We showed that the Si content significantly increased in the Si-treated Somyongkong (28%) and Taeseon (30%) compared to the control cultivars. Overall, our results suggested that Si affects root development as well as the genes involved in the auxin synthesis, transport pathway, and modulates root growth leading to cultivar-dependent variation in soybeans.

A hydroponic system was considered to be an appropriate method for root investigations, particularly if the method can be established as a screening technique to classify differences in root traits among sugarcane lines. The aim of the research was to classify root and shoot characters of sugarcane lines grown under hydroponic conditions and to investigate their correlation with the field measurements. Hydroponic and field tests used a randomized complete block design (RCBD). Eight elite sugarcane lines were assigned as treatments. Shoot and root traits were measured at 3 months after transplanting in the hydroponic experiment, and field trial measured root length density (RLD) at 4 months after planting. Root and shoot traits under the hydroponic system showed significant differences among sugarcane lines. KK3 had a high root surface area, root volume, root length and root dry weight and TBy27-1385 demonstrated a high stem height and stem dry mass. Moreover, root dry weight, root volume and root surface area positively correlated with shoot dry mass. Positive correlation was exhibited between root traits such as dry weight of root and root length derived from hydroponic studies and RLD derived from field conditions.

Temporal and spatial variations in ozone levels and temporal changes in solar radiation greatly influence ultraviolet radiation incidence to crops throughout their growth, yet the interactive effects of CO2 and UV-B radiation on Basil production under sunlight environmental conditions has not been studied. Basil ‘Genovese’ plants grown under sunlit plant growth chambers were subjected to a combination of supplemental UV-B (0 and 10 kJ m−2d−1) and ambient (420 ppm) and elevated (720 ppm) CO2 treatments for 38 days after 14 days of germination. UV-B radiation treatments caused a decrease in basil stem branching, fresh mass, and stem dry mass under both CO2 treatments when harvested after 17 and 38 days of treatment. There was also an increase in basil leaf surface wax under UV-B (10 kJ m−2d−1) treatment compared to controls (0 kJ m−2d−1). Elevated CO2 treatments caused a decrease in morphological features, including specific leaf area and fresh mass. Interactive effects between UV-B and CO2 treatments existed for some morphological features, including plant height, root surface area, and average root diameter. Understanding the impacts that CO2 and UV-B radiation treatments have on basilcan improve existing varieties for increased tolerance while simultaneously improving yield, plant morphology, and physiology.

Roots are the most important plant organ for absorbing essential elements, such as water and nutrients for living. To develop new climate-resilient soybean cultivars, it is essential to know the variation in root morphological traits (RMT) among diverse soybean for selecting superior root attribute genotypes. However, information on root morphological characteristics is poorly understood due to difficulty in root data collection and visualization. Thus, to overcome this problem in root research, we used a 2-dimensional (2D) root image in identifying RMT among diverse soybeans in this research. We assessed RMT in the vegetative growth stage (V2) of 372 soybean cultivars propagated in polyvinyl chloride pipes. The phenotypic investigation revealed significant variability among the 372 soybean cultivars for RMT. In particular, RMT such as the average diameter (AD), surface area (SA), link average length (LAL), and link average diameter (LAD) showed significant variability. On the contrary RMT, as with total length (TL) and link average branching angle (LABA), did not show differences. Furthermore, in the distribution analysis, normal distribution was observed for all RMT; at the same time, difference was observed in the distribution curve depending on individual RMT. Thus, based on overall RMT analysis values, the top 5% and bottom 5% ranked genotypes were selected. Furthermore, genotypes that showed most consistent for overall RMT have ranked accordingly. This ultimately helps to identify four genotypes (IT 16538, IT 199127, IT 165432, IT 165282) ranked in the highest 5%, whereas nine genotypes (IT 23305, IT 208266, IT 165208, IT 156289, IT 165405, IT 165019, IT 165839, IT 203565, IT 181034) ranked in the lowest 5% for RMT. Moreover, principal component analysis clustered cultivar 2, cultivar 160, and cultivar 274 into one group with high RMT values, and cultivar 335, cultivar 40, and cultivar 249 with low RMT values. The RMT correlation results revealed significantly positive TL and AD correlations with SA (r = 0.96) and LAD (r = 0.85), respectively. However, negative correlations (r = −0.43) were observed between TL and AD. Similarly, AD showed a negative correlation (r = −0.22) with SA. Thus, this result suggests that TL is a more vital factor than AD for determining SA compositions.

The aim of this study was to investigate the effects of exogenously applied auxin over the rootstock cuttings on root morphology parameters and stand establishment rate of salt-stressed cutting grafted cucumber seedlings. For that purpose, before grafting, the cut ends of the rootstocks were soaked for few seconds into auxin solution (indole-3-acetic acid (IAA) or indole-3- butyric acid (IBA))at different concentrations and afterwards were grafted by the root pruned splice grafting (RPSG) method. Ten days after grafting, the grafted seedlings were transplanted into individual pots where two different levels of salt-stress (0 and 50 mM) were established. Root morphology parameters, as well as dry matter of the root system and the whole plants were successively analysed to randomly selected plants. Exogenous auxins improved root morphology parameters and restored root growth under salinity conditions. The best results were obtained through the application of IBA, which promoted a better rootstock - scion relationship, presumably due to faster development of phloem and xylem tubes, and the promotion of a vigorous root system which increases plant's absorbing capabilities for water and nutrients under adverse soil conditions.

Karst regions are characterized by heterogeneous soil habitats, with shallow wide soil (SW) on hilly slopes and deep narrow soil (DN) in rocky trenches. To make full use of limited water and nutrients, plants have therefore developed a number of root extension strategies. This study investigated the effect of soil moisture on horizontal root extension in SW and vertical root extension in DN by assessing root growth responses, biomass allocation, and root distribution. A full two-way factorial blocked design of soil dimensions by water availability was followed. The perennial grass Lolium perenne L. was grown in SW and DN under high (W100%), moderate (W50%), and low (W30%) water availability, respectively. The main results were as follows: (1) The total biomass of L. perenne was not influenced either by soil habitat or by water application. Root length, root surface area, root biomass and root to shoot ratio all decreased with decreasing water application in SW, but not in DN soil. (2) With decreasing water application, the cumulative percentage of root length, root surface area and root biomass in 4 rings from the center out to 12 cm of SW soil showed a trend of W50% > W30% > W100% in SW, however, the cumulative percentage of root biomass in 4 layers from the surface to a depth of 36 cm was not significantly different between different water treatments in DN. (3) Under all three water treatments, specific root length showed an increase but root length density showed a decreasing trend from the center outward in SW soil or from the surface to bottom in DN soil. Overall, these results suggest that in SW habitat, soil moisture determines horizontal expansion of the roots in L. perenne, although the overall expansion ability was limited in severe drought. However, due to the relatively strong water retention ability, soil moisture changes were less obvious in DN, resulting in no significant vertical extension of the root system. The root response of L. perenne helps our understanding of how herbaceous plants can adjust their belowground morphology to support their growth in harsh karst soil environments.

The area of the root surface of human teeth has been investigated extensively in the dental literature. All previous attempts mainly rely on the use of physical methods to calculate surface area on extracted teeth or use virtual 3D Models for the same. The aim is to develop an algorithm using MATLAB software that estimates the dimensions of 3-D image produced with the help of CBCT so that the same can be utilized to calculate the root surface area of teeth among Indian population. Present research utilizes CBCT images of samples of extracted teeth mounted on a customized jpg. A descriptive chart for statistical analysis has been prepared to obtain average root surface area of each tooth type. The currently developed algorithm has been successfully applied to the CBCT images of complete sample of teeth to obtain their root surface area. The algorithm developed to calculate root surface area of the teeth holds wide spread application in the field of dentistry pursuing its high expediency in even various specializations of dentistry including orthodontics, prosthodontics, periodontology and implantalogy. It is concluded that it has now become a reality to accurately determine the surface area of the root of human teeth without extracting them using the CBCT radiographs of the patients.

Rainfall is the main resource of soil moisture in the semiarid areas, and the altered rainfall pattern would greatly affect plant growth and development. Root morphological traits are critical for plant adaptation to changeable soil moisture. This study aimed to clarify how root morphological traits of Bothriochloa ischaemum (a C4 herbaceous species) and Lespedeza davurica (a C3 leguminous species) in response to variable soil moisture in their mixtures. The two species were co-cultivated in pots at seven mixture ratios under three soil water regimes [80% (HW), 60% (MW), and 40% (LW) of soil moisture field capacity (FC)]. At the jointing, flowering, and filling stages of B. ischaemum, the LW and MW treatments were rewatered to MW or HW, respectively. At the end of growth season, root morphological traits of two species were evaluated. Results showed that the root morphological response of B. ischaemum was more sensitive than that of L. davurica under rewatering. The total root length (TRL) and root surface area (RSA) of both species increased as their mixture ratio decreased, which suggested that mixed plantation of the two species would be beneficial for their own root growth. Among all treatments, the increase of root biomass (RB), TRL, and RSA reached the highest levels when soil water content increased from 40 to 80% FC at the jointing stage. Our results implied that species-specific response in root morphological traits to alternated rainfall pattern would greatly affect community structure, and large rainfall occurring at early growth stages would greatly increase their root growth in the semiarid environments.

Adenophora triphylla var. japonica Hara is a highly valued medicinal plant that is used to treat or prevent bronchitis, cough, cancer, and obesity. However, there has been no study on the production of Adenophora triphylla var. japonica Hara seedlings in a closed-type plant production system (CPPS). This study was conducted to examine the growth characteristics of Adenophora triphylla var. japonica Hara seedlings as affected by different growing media. The seeds were sown on a 128-cell plug tray filled with urethane sponges (US), LC grow foam (LC), rockwool (RW), or terra-plugs (TP). The seedlings were cultured for a duration of 54 days under temperature 25 ± 1°C, a photoperiod of 12/12 h (light/dark), and light intensity of 180 µmol·m−2·s−1 photosynthetic photon flux density provided by RB LEDs (red:blue = 8:2) in a closed-type plant production system (CPPS). The germination rate of Adenophora triphylla var. japonica Hara was significantly highest in the TP. Also, seedling shoot growth indicators of plant height, leaf length, leaf width, number of leaves, fresh weight (FW), and dry weight (DW) of the shoot, and leaf area were markedly the greatest in the TP and the lowest in the US. The SPAD (soil-plant analysis development) value was higher in the TP and US than in the LC or RW. In addition, the seedling root growth characteristics of total root length, root surface area, root volume, and number of root tips were significantly greatest in the TP. Moreover, the maximum root diameter, FW and DW of roots were the greatest in the TP. In conclusion, the results suggest that TP are viable for the growth development of Adenophora triphylla var. japonica Hara seedlings.

Chilling is a major constraint in rice production in cool climates. In rice (Oryza sativa L.) plants, both the air temperature and the water (soil) temperature affect various growth processes independently, and low root zone temperature (thus, root temperature) can inhibit rice growth and yield. In this study, we investigated the effect of low root temperature on rice growth in relation to dry matter production and root water uptake. Plants were grown in hydroponic solutions at two temperatures, one equivalent to air temperature and the other 14ºC for 15 d starting 11 d after germination. Low temperature of the solution (low root temperature) inhibited dry matter production of rice plants by decreasing leaf area rather than photosynthetic rate. The response of leaf area was affected by changes in plant water status, that is relative water content (RWC) of stem was decreased by low root temperature resulting in reduced leaf area. The decrease in RWC caused by low root temperature was related to that in root hydraulic conductance (Kr). The responses of transpiration (E) and Kr to the low root temperature depended more on root surface area than on changes in hydraulic conductance per unit root surface area (Lpr). These results suggest that dry matter production under the low root temperature condition is controlled mainly by quantitative growth parameters such as leaf area and root surface area.

Conical crown-retained dentures (CCRD) show a higher survival rate and greater patient satisfaction than transitional removable partial dentures during long-term follow-up. However, unsustainable denture retention force on supporting abutments after initial delivery and loss retention are frequently seen in long-term follow-up of clinical cases. The main causes are insufficient information concerning denture retention designs and the retention-tolerance of the supporting abutments. Monitoring by dental technicians of the quality of dental prostheses is critical. This case report describes an optimal method for CCRD construction that determines and distributes an optimal denture retention force on the supporting abutments to allow the patient to easily remove the denture while ensuring that the CCRD remains in place during physiologic activities. Oral rehabilitation with CCRD should consider the condition of the abutment periodontal support, the interarch occlusal relationship, supplemental fatigue of the terminal abutment, and patient's estimated bite force. The effects of friction on the abutment's inner crown were based on an optimal a angle. The dental laboratory used these measurements to fabricate a CCRD using a Koni-Meter to adjust the retention of the inner crown. This method protects the abutments and reduces the wear between the inner and outer crowns. The CCRD achieved good esthetic results and physiologic functions. Periodic long-term follow-up of the patient and CCRD after initial placement is recommended.

In the field, plants show better root growth in drying soil than in wet soil. However, the root growth enhancement has not been demonstrated clearly in the laboratory. In this study, the root growth response of wheat seedlings to moderate soil water deficits was characterized quantitatively in an environment-controlled chamber. Germinated seeds of wheat were grown for 15 days in the soil with a water potential ranging from field capacity (FC) to approximately –0.08 MPa. Theleaf area decreased with reduction in soil water potential. By contrast, the root surface area increased upon reduction ofthe soil water potential to –0.04 MPa while it decreased significantly in soil with a water potential of –0.08 MPa. The increase in surface area was obvious in the roots with a diameter of 0.2 to 0.4 mm and larger than 0.7 mm. Root weight increased with the reduction of soil water potential to –0.04 MPa. While specific root length decreased significantly with the reduction of water potential to –0.06 MPa, the specific root surface area did not. Assimilatestransported from shoot might be used in roots to increae the surface area mainly by increasing the diameter rather than the length in response to a moderate soil water deficit in wheat seedlings. This might result from the drought tolerance mechanism of osmotic adjustment in roots.

Zinc (Zn) deficiency is a major constraint to rice production and Zn is also often deficient in humans with rice-based diets. Efforts to breed more Zn-efficient rice are constrained by poor understanding of the mechanisms of tolerance to deficiency. Here we assess the contributions of root growth and root Zn uptake efficiency, and we seek to explain the results in terms of specific mechanisms. We made a field experiment in a highly Zn-deficient rice soil in the Philippines with deficiency-tolerant and -sensitive genotypes, and measured growth, Zn uptake and root development. We also measured the effect of planting density. Tolerant genotypes produced more crown roots per plant and had greater uptake rates per unit root surface area; the latter was at least as important as root number to overall tolerance. Tolerant and sensitive genotypes took up more Zn per plant at greater planting densities. The greater uptake per unit root surface area, and the planting density effect can only be explained by root-induced changes in the rhizosphere, either solubilizing Zn or neutralizing a toxin that impedes Zn uptake (possibly HCO3- or Fe2+), or both. Traits for these and crown root number are potential breeding targets.