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Background: The lip print pattern is a unique among individuals and thus helps in the identification during the forensic investigation. Lip print analysis in the forensic scene may help in the personal identification. Thus, correlating the lip print with the finger print and gender will create a new room in forensic dentistry investigation. Aim: This study aims to identify the lip and finger print pattern and to correlate lip and finger print pattern for gender identification. Materials and Methods: A total of 100 students from dental institution were included in this study. Of which, 50 were male and 50 were female.. Their aged range between 18 and 28 years with a mean age of 21.3 years. The physiological rest position of the lip print was recorded by the application of red or dark pink colored lipstick over the lips and the imprint was transferred over a white chart sheet through a transparent tape. The imprint of the finger was taken directly on a white chart sheet. The lip and finger print pattern was examined with magnifying lens. The results were analyzed using SPSS software 19.0 (Armonk, NY, USA) and presented as a descriptive statistics. A correlation of lip print with finger print and gender variation was analyzed by Pearson correlation test. Results: The study identified higher prevalence of Type II (34%) lip print pattern in the overall study population. In males, Type I lip print (42%) and plain loop finger print pattern (44%) was frequent in the study. In males, the correlation of lip and finger print patter showed weaker correlation and statistically insignificant (P = 0.436). In females, Type II lip print (34%) and plain loop finger print pattern (42%) was frequent in the study. In females, the correlation of the lip and finger print pattern showed weaker correlation with approachable statistical significance (P = 0.083). The correlation of lip and finger print with gender showed weaker correlation and approachable significance (P = 0.07). The association of lip and finger print in the study showed statistically insignificant. However, the association of Type III lip print pattern with loop finger print pattern showed statistical significant association (P = 0.05) by Chi-square analysis. Conclusion: Lip print analysis is a challenging area in the personal identification during forensic dentistry examination. The study revealed the weaker correlation and approachable significance of lip and finger print pattern in gender identification. Future studies should be encouraged in the direction of software based identification for lip and finger print analysis in gender identification. Such studies may benefit this study pattern in more accurate way.

Background: Xylene is a flammable liquid with characteristic petroleum or aromatic odours, it is miscible with most of the organic solvents and paraffin wax. Xylene clears tissues rapidly and renders transparency, facilitating clearing endpoint determination, this made it to be used as a clearing agent in routine histopathological techniques. Even though it is a good clearing agent, it causes damage to the tissues by its hardening effect particularly those fixed in non-protein coagulant fixatives. Apart from these tissue effects, it has severe, long lasting ill effects on health of technicians and pathologists when exposed to longer duration. Hence in order to overcome these effects and replace xylene with a safe alternative agent, the present study was carried out to assess the clearing ability and biofriendly nature of four different natural oils i.e., Carrot oil, Olive oil, Pine oil and Rose oil in comparison with that of Xylene. According to Bernoulli’s principle of fluid dynamics, to decrease viscosity of these oils and increase penetration into tissues for rapid clearing hot-air oven technique was used. Aims: To assess:1) Clearing ability and bio-friendly nature of four different oils i.e., Carrot oil, Olive oil, Pine oil, Rose oil in comparison with that of xylene, 2) Application of Bernoulli’s principle of fluid dynamics in rapid clearing of tissues by using hot-air oven. Materials and Methods: Forty different formalin fixed tissue samples were taken. Each sample of tissue was cut into 5 bits (40x5=200 total bits) which were subjected for dehydration in differential alcohol gradients. Later, each bit is kept in 4 different oils such as Carrot oil, Olive oil, Pine oil, Rose oil and xylene and transferred into hot-air oven. Further routine steps of processing, sectioning and staining were done. Individual sections cleared in four different oils were assessed for cellular architecture, staining quality and a comparison was done between them. Results: Results showed that all the four oils had ability to clear the tissues similar to that of xylene. Pine oil was superior in its physical and clearing properties, which also maintained good cellular architecture and distinct staining quality, when compared with other oils and xylene. Conclusion: Carrot oil, Olive oil, Pine oil, Rose oil, are not only bio friendly and economical but can also be used as clearing agent instead of xylene. In this study stability and longetivity of haematoxyllin and eosin staining was evaluated for over a period of one year and no significant difference in staining quality were observed.