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Astaxanthin is an antioxidant that can be extracted from crustaceans such as shrimp, lobster, crawfish, and crabs. Fermented Acetes shrimp (called cincalok) is a traditional side dish from Indonesia rich in astaxanthin. This study extracted astaxanthin from cincalok using the glass beads vortex method with virgin coconut oil (VCO) as a green solvent. Several parameters that affect the amount of astaxanthin extracted, such as the ratio between sample and solvent, contact time, and particle size of glass beads, have been tested. The physicochemical characteristics of VCO before and after extraction were also analyzed. The UV-Vis spectrophotometer analysis results showed that the optimum ratio between sample and solvent was 1:10 g/mL, the optimum contact time was 15 minutes, and the optimum particle size of the glass beads was 60–80 mesh. Under these optimum conditions, the amount of astaxanthin extracted was 27.97 μg/g dry weight of cincalok or 2.79 μg/mL VCO. After extraction, the viscosity and density of VCO still meet the Indonesian National Standard with values of 17.98 mm2/s and 917.2 kg/m3, respectively. Before and after extraction, the GC-MS chromatogram shows the main component of fatty acids in VCO is lauric acid, with a percentage of 27.28 and 26.72%, respectively. VCO can also extract omega-3 fatty acids DHA and EPA from cincalok with the same rate of 0.04%.

Shrimp species have been reported to contain astaxanthin, which has high antioxidant activities. They also contain omega-3 in the form of docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), which can act as photoprotective agents that maintain healthy skin from reactive oxygen species (ROS) due to exposure to UV rays. In addition, fermentation has become an essential pre-treatment to extract the bioactive components in shrimp more easily. This study aims to extract oil from cincalok, a traditional Indonesian (especially in West Kalimantan) food made from Acetes shrimp fermented for 7–15 days. Cincalok oil was added to the lotion as a bioactive additive and sunscreen. Cincalok oil was extracted by the soxhletation method using n-hexane as solvent. The oil was then analyzed for its physicochemical properties, including density, viscosity, possible heavy metal contamination, and the profile of the fatty acids contained. The yield of cincalok oil extraction was 1.09 ± 0.05%, with the highest fatty acid content of 21.70% palmitic acid, 10.99% DHA, and 10.33% EPA. Cincalok oil also contains astaxanthin of 0.38 ± 0.02 mg/L oil. It has a viscosity of 69.71 ± 0.12 cP with a density of 0.93 ± 0.03 g/cm3. The analysis data of ICP-AES shows that there is no heavy metal contamination. The SPF value produced from cincalok oil lotion at 5 and 10% variations of cincalok oil was 15.17 ± 0.09 and 30.28 ± 0.49, respectively. The SPF value of lotion with the addition of cincalok oil was much greater than that of the base lotion, which was 2.16 ± 0.12.