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1. Activity-based protein profiling as a robust method for enzyme identification and screening in extremophilic Archaea

Author

Susanne Zweerink, Verena Kallnik, Sabrina Ninck, Sabrina Nickel, Julia Verheyen, Marcel Blum, Alexander Wagner, Ingo Feldmann, Albert Sickmann, Sonja-Verena Albers, Christopher Bräsen, Farnusch Kaschani, Bettina Siebers, and Markus Kaiser

Subjects
Science
Abstract

Activity-based protein profiling (ABPP) is a chemical proteomics method to profile activity states of enzymes under physiological conditions. Here the authors show that ABPP can be applied to archaeal serine hydrolases in the model organismSulfolobus acidocaldariusand can be used to identify novel putative serine hydrolases.

Published
2017
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