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Epigallocatechingallate (EGCG) in green tea extract has activity as an anti-inflammatory agent. On the other hand the stability of EGCG is poor because of the oxidation. The aim of this study was to determine the influence of Vitamine C and Vitamine E in formulation of green tea extract cream to the stabiliy of EGCG. The green tea extract was obtained from the extraction process by infundation followed by fractination with ethyl acetate as the solvent. The three formulas were compiled in similar composition with the concentration of vitamine C 1% (FI), Vitamine E 1% (FII) and there was no Vitamine C and Vitamine E (FIII) as a control. The EGCG level was determinated by TLC-densitometry methode. The stability parameter was determinated by calculated of the Q10 of each formula. The result of this study showed that the parameter of t90 of EGCG with Vitamine C 1%, Vitamine E 1% and control addition were 0.0108 hours, 0.0087 hours, 0.0084 hours, respectively. Stability of EGCG in green tea leaf extract cream with addition of the vitamin C 1% was higher than it stability with the addition of vitamin E 1%. The concentration of Vitamin C 1% was the optimum concentration as antioxidant in formulation of green tea extract cream.

Background and Objective: Reduction in cerebral blood flow following cereblal ischemia cause the production of oxygen free radicals and finally leads to brain tissue destruction. Pyramidal cells of the CA1 region of hippocampus are highly sensitive to hypoxic condition. This study was done to determine the effect of human chorionic gonadotropin (hCG) and vitamine E on cellular density of CA1 hippocampal area, learning ability and memory, following ischemia - reperfusion injury in mice. Materials and Methods: This experimental study was done on 40 male mice in 5 groups as follow: sham control, ischemia, hCG treated, vitamine E treated and hCG + vitamine E treated groups. Single dose of vitamin E was injected intraperitonaly during the establishment of reperfusion and hCG was injected from 48h after ischemia for 5 days. Folowing the treatment period, mice brains were fixated by transcardial perfusion and stained by nissle method. The shuttle box was used to evaluate the learning memory. Results: Co-administartion of vitamine E and hCG, significantly increased the cell numbers in hippocampus compared to the ischemic group (P

The steadily improved care of health leads to an increased interest in healthy nourishment. Apart from other angles, the nutriments and their raw materials are evaluated in compliance with the content of nutritively valuable substances. Barley, malt, malt extract and beer are sources of a number of chemical compounds that without any doubt have positive action on human organism - mainly the substances with antioxidant effect and some forms of dietetic fibres. In its department, the Malting Institute in Brno, the VUPS Ltd. engages in determination of content of tocols and b-glucans in individual grains of barley in dependence on the variety and genetic characters of the materials.The steadily improved care of health leads to an increased interest in healthy nourishment. Apart from other angles, the nutriments and their raw materials are evaluated in compliance with the content of nutritively valuable substances. Barley, malt, malt extract and beer are sources of a number of chemical compounds that without any doubt have positive action on human organism - mainly the substances with antioxidant effect and some forms of dietetic fibres. In its department, the Malting Institute in Brno, the VUPS Ltd. engages in determination of content of tocols and b-glucans in individual grains of barley in dependence on the variety and genetic characters of the materials.