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Objective To assess clinical efficacy of vedolizumab （VDZ） in the treatment of inflammatory bowel disease（IBD） and analyze whether VDZ combined with immunomodulator and/or corticosteroids can achieve better outcomes. Methods Twenty-nine patients with active IBD including 21 cases of ulcerative colitis （UC） and 8 cases of Crohn’s disease （CD） were recruited. They were assigned to receive VDZ （at a dose of 300 mg） intravenously at 0, 2, 6 weeks as induction therapy and every 8 or 4 weeks as maintenance therapy. The primary endpoint was clinical response at week 14/22, while the secondary endpoints were clinical remission and mucosal healing at week 54 （VDZ administration every 8 weeks） or week 30 （VDZ administration every 4 weeks）. Based on the concomitant medications, all patients were divided into the VDZ monotherapy and combination groups （VDZ combined with immunomodulator and/or corticosteroids）. The therapeutic effect was compared between two groups. Results Among UC patients,clinical response and endoscopic remission rates at week 14/22 were 81% （13/16） and 63% （10/16）, respectively. At week 54, clinical remission rate was 80% （4/5）and mucosal healing rate was 40% （2/5）. Among CD patients, clinical response rates at week 14/22 and week 30 were 75% （6/8） and 100% （1/1）, respectively. There was no significant difference in clinical response rate at week 14/22 between the VDZ monotherapy and combination groups. Conclusions VDZ is an effective biologic agent as induction and maintenance therapy for UC and CD. VDZ combined with immunomodulator and/or corticosteroids brings no additional benefit in the induction therapy. Therefore, VDZ monotherapy may be a better choice in clinical practice.

Objective To investigate the effect of infliximab （IFX） combination therapy on inflammatory biomarkers and intestinal mucosal healing in patients with extensive Crohn’s disease（CD）. Methods According to the evaluation criteria of endoscopic grading score and mucosal healing of Sun Yat-sen Memorial Hospital of Sun Yat-sen University, inflammatory biomarkers and mucosal healing data of 160 patients with extensive CD were retrospectively analyzed. All patients were assigned into the IFX combination therapy group （n = 98） and conventional drug therapy group （n = 62）. Results The C-reactive protein （CRP） level, erythrocyte sedimentation rate （ESR） and interleukin 6 （IL-6） level began to decline, but the serum TNF-α level increased during the second cycle of IFX treatment. In the IFX combination therapy group, the healing rate of colonic mucosa in patients treated for 12 times had statistical significance compared with that of those treated for less than 12 times （P < 0.05）. In the IFX combination therapy group, the endoscopic total effective rate was 80.3% （57/71）, significantly higher than 64.5% （40/62） in the conventional drug therapy group （P = 0.041）. Conclusions IFX combination regimen can significantly increase the serum TNF-α level, whereas it exerts no effect upon intestinal mucosal healing. IFX combination therapy can more effectively promote colonic mucosal healing than conventional therapy.

The present experiment was conducted to study the effects of different levels of oligosaccharide(FOS) and complex CPP preparation(CPP) in diets on growth performance and serum biochemical parameters for weaned piglets. 240 crossbred DLY( Duroc× Landrace× Yorkshire) 21-day-old weaning piglets, about 7.2 kg in initial body weight were selected and randomly divided into 6 groups, with 2 repeats for each group, 20 each repeat(half male and half female.). Group 1, as the control group, was fed base diet(BD), group 2 was fed BD adding 0.10% aureomycin, group 3, 4, 5, 6, respectively, was fed BD adding 0.05% CPP, 0.05% FOS, 0.20% FOS and 0.10% FOS + 0.05% of CPP. Feeding trial was conducted for 35 days. Results: (1) The combination of 0.10%FOS and 0.05%CPP(group 6) were added, the average daily gain of weaned pigs increased by 13.71 % (P < 0.05) and the feed conversion ratio was reduced by 7.41 % (P < 0.05) compared with control. The diarrhea rate of piglets decreased by 94.63 % (P < 0.01) compared with the control (p < 0.05), and decreased by 62.86 % (P 0.05).(3) There was no significant difference in serum biochemical indexes, such as serum total protein, albumin, urea nitrogen, cholesterol, triglycerides, alanine aminotransferase, aspartate aminotransferase and sugar by adding FOS and CPP individually or jointly in diet of weaned piglets (P > 0.05);there was no significant effect in serum immunoglobulin IgG and IgM (P> 0.05), but the serum IgE content was increased in different degrees. Conclusion: Adding FOS and CPP in diet for weaned piglets can improve the growth performance, reduce diarrhea rate of piglets, and promote digestion and absorption of feed nutrients, without significant difference between them and diet adding antibiotics. Among them, the effect of adding 0.10%FOS+0.05%CPP was the best.

The present experiment was conducted to study the effects of different levels of oligosaccharide(FOS) and complex CPP preparation(CPP) in diets on growth performance and serum biochemical parameters for weaned piglets. 240 crossbred DLY( Duroc× Landrace× Yorkshire) 21-day-old weaning piglets, about 7.2 kg in initial body weight were selected and randomly divided into 6 groups, with 2 repeats for each group, 20 each repeat(half male and half female.). Group 1, as the control group, fed base diet(BD) , group 2 fed BD adding 0.10% aureomycin, group 3, 4, 5, 6, respectively, fed BD adding 0.05% CPP, 0.05% FOS, 0.20% FOS and 0.10% FOS + 0.05% of CPP. Feeding trial was conducted for 35 days.Results: (1) Intestinal pH: Compared with the control group, the pH values of jejunum, ileum, cecum and colon in the 3, 4, 5 and 6 groups were significantly lower (P < 0.05), while there was no significant difference between the antibiotic group and the control group (P > 0.05).(2) Small intestinal villus morphology: Duodenal segment, villus length of 6 groups was significantly increased compared with the control group (P < 0.05), villus height to recess depth ratio (V/C) was significantly increased (P < 0.05). There was no significant difference in villus length between groups in jejunal segment (P > 0.05). Groups five V/C was significantly higher than that in the control group (P < 0.05). Compared with the control group and the antibiotic group, the ileal segment in the six groups significantly increased V/C (P < 0.05).(3) Intestinal flora Adding FOS, CPP and their combination could reduce the number of E. coli and Salmonella in different intestinal segments, and increase the number of Lactobacillus and Bifidobacteria. Among them, the effect of joint addition is the most obvious. Conclusion: Adding FOS and C PP to the diet of weaned piglets could effectively reduce the intestinal pH value of piglets, increase the length of intestinal villi and V/C, reduce the number of harmful bacteria and increase the number of beneficial bacteria. It is beneficial to maintain the balance of intestinal morphology and microflora and improve the intestinal health of piglets. Among them, the combination of 0.10% FOS and 0.05% CPP is the best, followed by 0.20% FOS.

24-week-old local yellow broiler breeder hens were selected to study the effects of dietary fructo-oligosaccharides(FOS)or antibiotics(bacitracin zinc)on laying performance, serum biochemical indices and dietary mutrient metabolizability. The results showed that: compared with the control group, dietary FOS supplemented with 0.2%, 0.4%, 0.6% could increase the laying rate, average egg weight, feed egg ratio, eggs percent of pass, fertility rate and hatchability of qualified egg, and reduce serum levels of total cholesterol and triglyceride, and improve the apparent metabolizability of dietary nutrients. The effects of diet supplemented with FOS was better than those of diet supplement with 0.05% antibiotics.It was suggested that the appropriate additive amount of FOS in diets for yellow broiler breeder hens should be 0.4%.