1. Gene ontology analysis of the TNF signaling pathway in early orthodontic tooth movement of rats with periodontitis
- Author
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MA Yao, JIANG Zhaowei, JIN Yunyi, MIAO Qian, ZHANG Chunxiang, and ZHANG Linkun
- Subjects
orthodontic tooth movement ,periodontitis ,rats ,inflammatory response ,tumor necrosis factor ,nf-kb transcription factor ,signaling pathway ,gene ontology analysis ,Medicine - Abstract
Objective To investigate the expression and function of the TNF signaling pathway in the early stage of orthodontic tooth movement with periodontitis and to provide evidence to study the early inflammatory response in patients with periodontitis orthodontic treatment. Methods Sixteen SD rats were randomly divided into four groups: group A--12 h of orthodontic tooth movement of the bilateral maxillary first molars in rats with periodontitis; group B--periodontitis model of the bilateral maxillary first molars without orthodontic tooth movement; group C--12 h of orthodontic tooth movement of the same teeth in rats with healthy periodontium; group D--control group without operations. The bilateral maxillary first molars and surrounding periodontal tissue of each group were collected for gene chip detection. Pathway enrichment analysis, qRT-PCR and GO (gene ontology) analysis were performed to identify differential genes involved in the TNF signaling pathway. Results Gene chip results showed that the TNF signaling pathway was significantly upregulated in group A, group B and group C (P group B > group C (P
- Published
- 2019
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