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To investigate the effects of different moisture contents on the storability of lightly salted large yellow croaker at 4 ℃, changes in total volatile basic nitrogen (TVB-N) content, microbial community composition, taste and volatile organic compounds were analyzed during the storage period, and the correlation between microbial community composition and VOCs was examined. The results showed that the shelf-life of lightly salted large yellow croaker with moisture contents of 60%, 55%, and 50% was 27, 33, and 39 days, respectively. During storage, there were significant changes in the bacterial flora, with four specific spoilage organisms (SSOs) being dominant at the end of the shelf-life. In the samples with 60% moisture content, Hafnia alvei (61.22%) and Brochothrix thermosphacta (19.39%) were dominant. In the samples with 55% moisture content, H. alvei (31.13%), B. thermosphacta (29.25%), Carnobacterium divergens (17.92%) and Citrobacter freundii (16.98%) were dominant. C. freundii was not observed at the end of the shelf-life of lightly salted large yellow croaker with 50% moisture content, and H. alvei (43.96%) and B. thermosphacta (37.36%) were dominant, respectively.The electronic tongue results showed that the trend of response values was basically the same among the three groups of samples. A total of 29 VOCs were detected by gas chromatography-ion mobility spectrometry (GC-IMS), including seven alcohols, six esters, four ketones, four aldehydes, three amines, two heterocycles, one olefin, one ether and one terpene. The fingerprinting results showed no significant difference in the composition of VOCs among the groups. Pearson’s correlation analysis showed that H. alvei and B. thermosphacta were positively correlated with amines for all groups.

Colorectal cancer (CRC) ranks the 3rd in cancer types globally. Long noncoding RNAs (lncRNAs) are related to the initiation and progression of CRC. The current study plans to reveal the action of rhabdomyosarcoma 2-associated transcript (RMST) in CRC. The results show that RMST is downregulated in CRC specimens and cell lines relative to normal specimens and a fetal normal colon cell line (FHC), respectively. Elevation of RMST represses cell proliferation and colony formation and induces cell apoptosis in CRC cells. Bioinformatic analysis reveals a binding site in RMST for miR-27a-3p. The direct association between RMST and miR-27a-3p is confirmed by dual luciferase reporter assay, RNA pull-down assay, and real time-quantitative polymerase chain reaction (RT-qPCR). miR-27a-3p is upregulated in CRC tumor specimens relative to normal specimens, and there is a negative correlation between RMST and miR-27a-3p in CRC tumor specimens. In addition, the effects of RMST overexpression are weakened by the elevation of miR-27a-3p. RMST and retinoid X receptor (RXRα) share the same complementary site with miR-27a-3p. The direct association between RXRα and miR-27a-3p is confirmed by RNA pull-down assay, RT-qPCR and western blot analysis. Overexpression of RMST induces RXRα expression and inactivates the Wnt signaling pathway by decreasing β-catenin levels in CRC cells. Collectively, our findings reveal a pivotal role of RMST in regulating miR-27a-3p/RXRα axis and counteracting Wnt signaling pathway during the progression of CRC.

The effect of ferrovanadium inoculation on the microstructure and properties of high speed steel (HSS) used for rolls was studied. The results showed that the as-cast eutectic carbide network tends to be broken after ferrovanadium inoculation, and the carbides are changed to rod-like or nodular shape. After heat treatment, the carbides in the inoculated HSS are spheroidized and distributed more uniformly in the matrix. The impact toughness of high speed steel with ferrovanadium inoculation is obviously improved. The action mechanism of ferrovanadium inoculation on the microstructure of the alloy is also discussed.