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In this study, we achieved the biosynthesis of novel 7–8 nm iron-oxide nanoparticles in the presence of different concentrations (5 to 50% w/v) of commercial white quinoa extract. Initially, quinoa extract was prepared at various concentrations by a purification route. The biosynthesis optimization was systematically monitored by X-ray diffraction, and the Rietveld quantitative analysis showed the presence of goethite (5 to 10 wt.%) and maghemite phases. The first phase disappeared upon increasing the organic loading (40 and 50% w/v). The organic loading was corroborated by thermogravimetric measurements, and it increased with quinoa extract concentration. Its use reduces the amount of precipitation agent at high quinoa extract concentrations with the formation of magnetic nanoparticles with hard ferrimagnetic character (42 and 11 emu g−1). The enrichment of hydroxyl groups and the negative zeta potential above pH = 7 were corroborated by a reduction in the point of zero charge in all the samples. For alkaline values, the zeta potential values were above the stability range, indicating highly stable chemical species. The evidence of hydroxyl and amide functionalization was qualitatively observed using infrared analysis, which showed that the carboxyl (quercetin/kaempferol), amide I, and amide III chemical groups are retained after biosynthesis. The resultant biosynthesized samples can find applications in environmental remediation due to the affinity of the chemical agents present on the particle surfaces and easy-to-handle them magnetically.

Introduction: Fermented foods, probiotic, prebiotics, and symbiotic, are among the most important groups of functional food that have attracted the attention of researchers during the last years. Proteolytic activity of lactic acid bacteria can lead to the production of peptides in the fermented product. The produced peptides can exhibit different biological activities such as antioxidant, antihypertensive, etc. that are influenced by the type of protein source, type of bacteria, time and conditions of fermentation process. Fermentation of various cereals such as quinoa seeds with high sugar and protein content by lactic acid bacteria can lead to the production of antioxidant peptides and improving their nutritional properties. Materials and Methods: In this study, the role of Lactobacillus reuteri and Lactobacillus acidophilus and the combination of two bacteria on the progress of fermentation and antioxidant activity of quinoa extract was investigated. The fermentation process was started by separate and simultaneous inoculating of Lactobacillus reuteri and Lactobacillus acidophilus and continued for 72 hours at 37° C. Sampling was performed every 24 hours of fermentation and samples were kept at -20° C for further analysis. The parameters such as pH, acidity, amount of soluble protein, degree of hydrolysis, amount of phenolic compounds and DPPH and ABTS radical scavenging activity were determined. Results and Discussion: Lactobacillus acidophilus showed higher acidification capacity than Lactobacillus reuteri. The amount of acidity in the sample fermented by Lactobacillus acidophilus increased from 0.27 to 1.13 % after 72 hours, while this amount was measured as 0.80 % for sample fermented by Lactobacillus reuteri. The amount of soluble protein and the degree of hydrolysis increased in samples fermented by both species. However, the largest increase was related to the sample fermented by Lactobacillus reuteri, so that the amount of soluble protein increased from 0.72 to 0.88 mg / ml and the value of free amino groups increased from 20.28 to 58.14 µM leucin/ mg protein during 72 hours of fermentation. The DPPH and ABTS radical scavenging activity increased in all fermented samples. The highest antioxidant activity was observed in samples fermented by Lactobacillus reuteri, followed by a combination of two bacteria (50:50) and Lactobacillus acidophilus. The amounts of phenolic compounds increased in all fermented samples. However, the highest increase was related to the sample fermented by Lactobacillus reuteri, so that it increased from 0/73 to 16.21 mg Gallic acid / ml after 72 hours of fermentation. Therefore, the results showed that despite the higher acidifying power of Lactobacillus acidophilus in quinoa extract, but Lactobacillus reuteri exhibited higher proteolytic activity, more ability to produce antioxidant peptides and also release phenolic compounds during the fermentation process.Simultaneous use of the two bacteria did not intensify the proteolytic activity and antioxidant activity of peptides, and the greatest increase in acidity, proteolysis, and antioxidant activity occurred in the first 24 hours of fermentation. Fermented extracts showed higher ABTS radical inhibitory activity than DPPH radical inhibition, indicating the hydrophilic nature of most produced antioxidant compounds. The highest levels of antioxidant activity were observed in samples fermented by Lactobacillus reuteri, a combination of Lactobacillus reuteri and Lactobacillus acidophilus (50:50) and Lactobacillus acidophilus, respectively. The results showed that fermentation by Lactobacillus reutri has the greatest effect on the production of antioxidant peptides and the release of phenolic compounds. The results of this study confirm the effectiveness of fermentation methods on improving the healing properties of quinoa extract and Lactobacillus reuteri was a more effective bacterium in fermentation and production of antioxidant peptides compared to Lactobacillus acidophilus. Simultaneous use of two bacteria did not increase the intensity of fermentation and did not improve the antioxidant activity compared to single use of each bacteria. Finally, the results of this study showed that fermentation of quinoa extract improves its antioxidant properties and has the potential to be used as a fermented beverage.