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Pulsed-field gel electrophoresis (PFGE) remains the tool for transmission and outbreak tracing. However, inter-laboratory comparison of PFGE could not be made due to the lack of a standard for interpretation and congruence profiles assignment. This study aims to overcome the shortcoming of PFGE profiling, as well as human performance in data analysis by developing a web-based tool for monitoring multidrug-resistant organisms (MDRO) and tracking the transmission based on PFGE. A web-based MDRO monitoring and transmission tracking system was developed based on three main subsystems. To build the database, banding patterns of PFGE were converted into string data using the box-based fragmentation method. The data could be visualized based on different categories and the transmission tracing could be achieved by the coexistence of the similar profile. This system could be used for monitoring the genotypic and phenotypic trends of MDRO and to trace potential outbreaks in hospitals.

Transfer RNA (tRNA), a key component of the translation machinery, plays critical roles in stress conditions and various diseases. While knowledge regarding the importance of tRNA function is increasing, its biological roles are still not well understood. There is currently no comprehensive database or web server providing the expression landscape of tRNAs across a variety of human tissues and diseases. Here, we constructed a user-friendly and interactive database, DBtRend, which provides a profile of mature tRNA expression across various biological conditions by reanalyzing the small RNA or microRNA sequencing data from the Cancer Genome Atlas (TCGA) and NCBI’s Gene Expression Omnibus (GEO) in humans. Users can explore not only the expression values of mature individual tRNAs in the human genome, but also those of isodecoders and isoacceptors based on our specific pipelines. DBtRend provides the expressed patterns of tRNAs, the differentially expressed tRNAs in different biological conditions, and the information of samples or patients, tissue types, and molecular subtype of cancers. The database is expected to help researchers interested in functional discoveries of tRNAs.

Efficient use of natural resources especially in Natura 2000 sites is an essential component of Europe 2020 strategy. The use of web database is absolutely necessary for a good resource management and it will provide a good communication channel for the main stakeholders: protected area manager, scientists, resources evaluators and local community. Access to information from database is allowed according with the user competence. General information on natural resources uses in Danube Delta Biosphere Reserve (D.D.B.R.) will be freely available. Different degree of information, especially regarding editing data will be applied for the main actors involved in use of natural resources. Evaluators that are mainly scientists with good biodiversity background, protected area staff that applies the regulation regarding natural resources in relation with ecological conditions, private companies or persons interested in harvesting natural resources. The user interface is realized by using OpenSource products. The web interface for tabular data was build using ExtJs Javascrip library. The web map user interface was build using Openlayers, GeoExt, and Ext. For database SQL server we chose PostgresSQL and GeoServer for maps server