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8 results on '"Bourboulia, Dimitra"'

1. Transformation of human mesenchymal stem cells increases their dependency on oxidative phosphorylation for energy production

Author

Funes, Juan M., Quintero, Marisol, Henderson, Stephen, Martinez, Dolores, Qureshi, Uzma, Westwood, Claire, Clements, Mark O., Bourboulia, Dimitra, Pedley, R. Barbara, Moncada, Salvador, and Boshoff, Chris

Subjects
Glycolysis -- Research, ATP synthesis -- Research, Oxidative phosphorylation -- Research, Stem cell research, Science and technology
Abstract

An increased dependency on glycolysis for ATP production is considered to be a hallmark of tumor cells. Whether this increase in glycolytic activity is due mainly to inherent metabolic alterations or to the hypoxic microenvironment remains controversial. Here we have transformed human adult mesenchymal stem cells (MSC) using genetic alterations as described for differentiated cells. Our data suggest that MSC require disruption of the same pathways as have been shown for differentiated cells to confer a fully transformed phenotype. Furthermore, we found that MSC are more glycolytic than primary human fibroblasts and, in contrast to differentiated cells, do not depend on increased aerobic glycolysis for ATP production during transformation. These data indicate that aerobic glycolysis (the Warburg effect) is not an intrinsic component of the transformation of adult stem cells, and that oncogenic adaptation to bioenergetic requirements, in some circumstances, may also rely on increases in oxidative phosphorylation. We did find, however, a reversible increase in the transcription of glycolytic enzymes in tumors generated by transformed MSC, indicating this is a secondary phenomenon resulting from adaptation of the tumor to its microenvironment. adult stem cells | glycolysis | Warburg effect

Published
2007

2. LIM domains-containing protein 1 (LIMD1), a tumor suppressor encoded at chromosome 3p21.3, binds pRB and represses E2F-driven transcription

Author

Sharp, Tyson V., Munoz, Fernando, Bourboulia, Dimitra, Presneau, Nadege, Darai, Eva, Wang, Hsei-Wei, Cannon, Mark, Butcher, David N., Nicholson, Andrew G., Klein, George, Imreh, Stephan, and Boshoff, Chris

Subjects
Lung cancer -- Research, Proteins -- Research, Science and technology
Abstract

LIM domains-containing protein 1 (LIMD1) is encoded at chromosome 3p21.3, a region commonly deleted in many solid malignancies. However, the function of LIMD1 is unknown. Here we show that LIMD1 specifically interacts with retinoblastoma protein (pRB), inhibits E2F-mediated transcription, and suppresses the expression of the majority of genes with E2F1-responsive elements. LIMD1 blocks tumor growth in vitro and in vivo and is down-regulated in the majority of human lung cancer samples tested. Our data indicate that LIMD1 is a tumor-suppressor gene, the protein product of which functionally interacts with pRB and the loss of which promotes lung carcinogenesis. lung cancer | retinoblastoma

Published
2004

3. Prospective study of the effects of antiretroviral therapy on Kaposi sarcoma-associated herpesvirus infection in patients with and without Kaposi sarcoma

Author

Gill, Jasjit, Bourboulia, Dimitra, Wilkinson, John, Hayes, Peter, Cope, Alethea, Marcelin, Anne-Genevieve, Calves, Vincent, Gotch, Frances, Boshoff, Christopher, and Gazzard, Brian

Subjects
Kaposi's sarcoma -- Care and treatment, Kaposi's sarcoma -- Genetic aspects, Kaposi's sarcoma -- Health aspects, Herpesvirus diseases -- Care and treatment, Herpesvirus diseases -- Causes of, Herpesvirus diseases -- Health aspects, Herpesvirus diseases -- Genetic aspects, Herpes, Epidemiology -- Research, Epidemiology -- Statistics, HIV patients -- Drug therapy, HIV patients -- Health aspects, HIV patients -- Care and treatment, HIV infection -- Prevention, HIV infection -- Research, HIV infection -- Health aspects, Antiviral agents -- Physiological aspects, AIDS (Disease) -- Research, Health
Abstract

Research has been conducted on Kaposi sarcoma-associated herpesvirus infection. The role of active antiretroviral therapy in circulating Kaposi sarcoma-associated herpesvirus load in HIV-infected people with or without Kaposi sarcoma has been investigated, and the results demonstrate the existence of relationship between HIV load response to active antiretroviral therapy and the clinical improvement of Kaposi sarcoma.

Published
2002

4. Antibodies against human herpesvirus 8 in black South African patients with cancer

Author

Sitas, Freddy, Carrara, Henri, Beral, Valerie, Newton, Rob, Reeves, Gillian, Bull, Diana, Jentsch, Ute, Pacella-Norman, Rosana, Bourboulia, Dimitra, Whitby, Denise, Boshoff, Chris, and Weiss, Robin

Subjects
Human herpesvirus 8 -- Statistics, Kaposi's sarcoma -- Physiological aspects
Abstract

Human herpesvirus 8 (HHV-8) is very strongly linked to Kaposi's sarcoma in South Africa. Among 3,344 black South Africans who had cancer, 51 had Kaposi's sarcoma, which is a type of skin tumor common in AIDS patients. Blood tests revealed that 83% of these 51 patients had antibodies against HHV-8 compared to 32% of the patients with other types of cancer. However, the presence of HHV-8 did not seem to be associated with HIV infection.

Published
1999

5. Seroprevalence of Human Herpesvirus 8 and Its Association With Kaposi Sarcoma in Brazil

Author

Zago, Adriana, Bourboulia, Dimitra, Viana, Maria Carmen, Collandre, Helene, Dietze, Reynaldo, Boshoff, Chris, and Keller, Regina

Subjects
Human herpesvirus 8 -- Health aspects, Kaposi's sarcoma -- Brazil, Health
Abstract

Background: Epidemiologic studies have indicated that human herpesvirus 8 is implicated in the development of Kaposi sarcoma in different geographic areas worldwide. Goal: To provide information on the prevalence of human herpesvirus 8 in Brazil and its association with Kaposi sarcoma. Study Design: An immunofluorescence assay was performed to test 1,044 serum samples from 747 blood donors, 73 patients presenting to casualty departments, and 224 patients attending sexually transmitted disease/AIDS clinics. The sexually transmitted disease group was composed of 88 patients with HIV (40 Kaposi sarcoma positive, 48 Kaposi sarcoma negative) and 136 patients without HIV. Results: Antibodies to human herpesvirus 8-latent nuclear antigens were found in 34 blood donors (4.6%), and in seven casualty patients (9.6%). The highest frequency of human herpesvirus 8 antibodies was found in the sexually transmitted disease group: 32 HIV-positive patients with Kaposi sarcoma (80%) and seven patients without Kaposi sarcoma (14.6%). Conclusion: The presence of human herpesvirus 8 in patients with HIV was strongly associated with Kaposi sarcoma (odds ratio, 23.4; 95% CI, 7.7-71.4), male gender, homosexual or bisexual orientation, and hepatitis B virus infection, but not with the other sexually transmitted diseases that were investigated.

Published
2000

6. Viral coinfections among African children infected with human immunodeficiency virus type 1. (HIV/AIDS: Brief Report)

Author

Chakraborty, Rana, Rees, Gareth, Bourboulia, Dimitra, Cross, Alexandra M., Dixon, Jedediah R., D'Agostina, Angelo, Musoke, Rachel, Boshoff, Chris, Rowland-Jones, Sarah L., and Klenerman, Paul

Subjects
HIV infection in children -- Complications, AIDS (Disease) in children, Cytomegaloviruses -- Research, Cytomegalovirus infections -- Physiological aspects, T cells -- Physiological aspects, Comorbidity -- Research, Hepatitis G -- Physiological aspects, Herpesviruses -- Physiological aspects, Kaposi's sarcoma -- Physiological aspects, Hepatitis viruses -- Physiological aspects, Health, Health care industry
Published
2003

7. Seroepidemiology and Molecular Epidemiology of Kaposi's Sarcoma-Associated Herpesvirus Among Jewish Population Groups in Israel

Author

Davidovici, Batya, Karakis, Isabella, Bourboulia, Dimitra, Ariad, Samuel, Zong, Jian-Chao, Benharroch, Daniel, Dupin, Nicolas, Weiss, Robin, Hayward, Gary, Sarov, Batia, and Boshoff, Chris

Subjects
Kaposi's sarcoma -- Demographic aspects, Prevalence studies (Epidemiology) -- Analysis, Human herpesvirus 8, Health
Abstract

Background: The incidence of classic Kaposi's sarcoma among Jews in Israel is among the highest in the developed world. Kaposi's sarcoma-associated herpesvirus (KSHV or human herpesvirus 8) is causally linked to Kaposi's sarcoma. Very little is known about the prevalence of KSHV in the Middle East or about the modes of transmission in Mediterranean countries. Methods: From 1992 through 1995, sera were obtained from 1648 adults who had tested positive for hepatitis B virus (HBV) surface antigen 20 years earlier at blood donations; sera were also obtained from 2403 of their family members. All sera were tested for anti-KSHV antibodies with the use of an indirect immunofluorescence assay. To analyze the effects of various factors on the risk of KSHV infection for both the HBV-positive cohort and their families, logistic regression for cluster data and generalized estimating equations were used. All statistical tests were two-sided. Results: Among family members, the seroprevalence of antibodies against KSHV was 9.9% (95% confidence interval [CI] = 8.7% to 11.1%); among the former blood donors who had tested positive for hepatitis B, it was 22% (95% CI - 19.9% to 24.1%). Overall, the best predictor of KSHV status was the place of birth. The most important risk factors found for both husband and wife to test KSHV positive were their own places of birth and their spouse's seropositivity. For a child to test positive, the most important risk factor was maternal seropositivity. Conclusions: The crude prevalence rate of KSHV among the Jewish population in Israel is 9.9%. Important routes of KSHV transmission in the families studied are spouse to spouse and mother to child. The presence of KSHV in Jews in Israel of all ethnic origins and their high incidence of reported Kaposi's sarcoma suggest that KSHV was introduced into the Jewish population prior to the major Diaspora. [J Natl Cancer Inst 2001;93: 194-202]

Published
2001

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