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The nucleotide sequence and location of the genes that mediate the synthesis of catechol from aniline was analyzed in the Pseudomonas putida UCC22 mt-2 chromosome. Introduction of the plasmid pTDN1-1016 into Pseudomonas putida UCC22 enabled the host to grow or metabolize methylated amines such as aniline and m-toluidine. Nucleotide sequence of the 9,233-bp HindIII (HC) fragment of pTDN1 indicated the presence of protein-encoding open reading frames which were designated as tnpA. Furthermore, the gene cluster in the HC fragment encoded large and small subunits of a terminal dioxygenase.

The 2,4-dichlorophenoxyacetic acid (2,4-D)/alpha-ketoglutarate dioxygenase (TfdA) of Alcaligenes eutrophus was analyzed by spectrophotometric assay with 4-nitrophenoxyacetic acid (4-NPAA). Spectrophometric analysis of the 2,4D-catalyzing enzymes of Alcaligenes eutrophus produced negative results for 4-NPAA degradation and exhibited no activity in the extracts. Furthermore, the TfdA isolates from Alcaligenes eutrophus did not exhibit color production in selected whole cells due to the inability of these cultures to transport 4-NPAA.