1. Intervening sequences in an Archaea DNA polymerase gene
- Author
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Perler, Francine B., Comb, Donald G., Jack, William E., Moran, Laurie S., Qiang, Boqin, Kucera, Rebecca B., Benner, Jack, Slatko, Barton E., Nwankwo, Donald O., Hempstead, S. Kay, Carlow, Clotilde K.S., and Jannasch, Holger
- Subjects
DNA polymerases -- Genetic aspects ,Archaeabacteria -- Genetic aspects ,Introns -- Identification and classification ,RNA splicing -- Research ,Post-translational modification -- Research ,Science and technology - Abstract
The Vent DNA polymerase was isolated from the extreme thermophile, Thermococcus litoralis. The gene coding for this polymerase was isolated, cloned and characterized. Sequence analysis showed the presence of intervening sequences (IVSs) making up 55% of the polyerase gene. One of these IVSs codes for the intron I endonuclease. Further analyses of these IVSs showed that they are removed either by protein of RNA splicing events that require the action of the endonuclease. This is the first report of introns within an open reading frame in either the Archaea or eubacteria.
- Published
- 1992