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1. Mutagenesis of Trp(sup)54 and Trp(sup)203 residues on Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase significantly affects catalytic activities of the enzyme

Author

Hsueh-Ling Cheng, Li-Chu Tsai, Su-Shiang Lin, Hanna S Yuan, Ning-Sun Yang, Shu-Hua Lee, and Lie-Fen Shyur

Subjects
Mutagenesis -- Analysis, Amino acids -- Structure-activity relationships, Enzyme kinetics -- Analysis, Catalysis -- Analysis, Biological sciences, Chemistry
Abstract

Results reveal that among the nine tryptophan residues investigated, residues Trp(sup)54 and Trp(sup)203 may play an important role in mediating the catalytic function of Fibrobacter succinogenes 1,3-1,4-beta-D-glucanase. Besides, Trp(sup)141 and Trp(sup)148 residues are involved in maintaining the structural integrity of the substrate binding cleft of the enzyme.

Published
2002

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