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Author(s): Salim S. Abdool Karim 1,2,*, Segenet Kelemu 3, Cheryl Baxter 1 Introduction The Coronavirus Disease 2019 (COVID-19) pandemic has dramatically changed everyone's lives, highlighting the fragility of systems providing [...]

Two sets of independently regulated pectate lyase isozymes capable of macerating plant tissues were produced by Erwinia chrysanthemi EC16. Mutant CUCPB5012, which directs mutations in all the known enzymes in all clearing alpha-1,4 galacturonosyl linkages, is constructed to seek the unknown components of the pectolytic activity of E.chrysanthemi EC16. A novel set of pel isozymes in inoculated chrysanthemum cuttings and in minimal medium supplemented with crysanthemum cell walls is produced by the mutant which retains some ability to macerate chrysanthemum tissues.