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Alkaloids and lignans in Zanthoxylum armatum display important biological activities, but the quanitification method of alkaloids has not been reported. In this study, an effective extraction method was developed through an orthogonal design, and ten compounds in roots, stems, branches and leaves of Zanthoxylum armatum were simultaneously quantified by ultra-fast liquid chromatography-tandem quadrupole mass spectrometry (UFLC-MS/MS). The roots, stems, branches and leaves of Z. armatum were ultrasonically extracted with methanol (solvent-to-sample ratio 100 : 1, v/w) for 10 min. An UFLC-MS/MS method was developed with a gradient UFLC mobile phase and triple quadruple tandem mass spectrometry with electrospray ionization in the positive ion mode. The method was validated for linearity, precision, repeatability and accuracy. The limits of detection and quantification were within 0.01-7.5 and 0.04-30 ng/mL, respectively. The root samples collected from Tian'e County were abundant in N-methylanhydrotetrahydroberberrubine A, escholidine perchlorate and pinoresinol monomethyl ether, eudesminthe., Author(s): Yi-ran Wang [sup.1], Shi-ying Mai [sup.1], Yong-hui Li [sup.1], Hai-long Li [sup.1], Tao Guo [sup.3], An Jia [sup.2], Jun-qing Zhang [sup.1] [sup.2] Author Affiliations: (1) grid.443397.e, 0000 0004 0368 [...]