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1. The S-layer gene of Lactobacillus helveticus CNRZ 892: Cloning, sequence and heterologous expression

Author

Callegari, M. Luisa, Riboli, Barbara, Willem Sanders, J., Sandro Cocconcelli, P., Kok, Jan, Venema, Gerard, and Morelli, Lorenzo

Subjects
Lactobacillus -- Physiological aspects, Monomers -- Observations, Proteins -- Observations, Microbiological research -- Observations, Biological sciences
Abstract

Surface layers (S-layers), consisting of protein monomers arranged in crystalline arrays have been described on Lactobacillus cell walls. Freeze-drying is thought to induce the detachment of S-layer monomers from the Lactobacillus acidophilus cell wall, although structural damaged is not linked to loss of cell viability. The cloning and nucleotide sequence of the gene encoding the S-layer protein of L. helviticus CNRZ 892, is presented, along with its expression in Lactococcus lactis, the Gram-positive heterologous host.

Published
1998

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