1. Innovative electrochemical approach for an early detection of microRNAs
- Author
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Lusi, E.A., Passamano, M., Guarascio, P., Scarpa, A., and Schiavo, L.
- Subjects
Electrochemistry -- Research ,RNA -- Electric properties ,RNA -- Chemical properties ,Biological markers -- Research ,Serum -- Properties ,Serum -- Genetic aspects ,Blood -- Medical examination ,Blood -- Methods ,Chemistry - Abstract
The recent findings of circulating cell-free tissue specific microRNAs in the systemic circulation and the potential of their use as specific markers of disease highlight the need to make microRNAs testing a routine part of medical care. At the present time, microRNAs are detected by long and laborious techniques such as Northen blot, RT-PCR, and microarrays. The originality of our work consists in performing microRNAs detection through an electrochemical genosensor using a label-free method. We were able to directly detect microRNAs without the need of PCR and a labeling reaction. The test is simple, very fast and ultrasensitive, with a detection limit of 0.1 pmol. Particularly feasible for a routine microRNAs detection in serum and other biological samples, our technical approach would be of great scientific value and become a common method for simple miRNAs routine detection in both clinical and research settings.
- Published
- 2009