1. Molecular detection of Norwalk-like calciviruses in sewage
- Author
-
Lodder, W.J., Vinje, J., Heide, R. van de, Husman, A.M. de Roda, Leenen, E.J.T.M., and Koopmans, M.P.G.
- Subjects
Molecular microbiology -- Research ,Viruses -- Genetic aspects ,Polymerase chain reaction -- Usage ,Sewage -- Physiological aspects ,Drinking water -- Health aspects ,Reverse transcriptase -- Usage ,Enteritis -- Causes of ,Cladistic analysis -- Usage ,Rotavirus infections -- Research ,Swimming -- Health aspects ,Viral diarrhea -- Causes of ,Biological sciences - Abstract
High loads of Norwalk-like viruses (NLVs) may be in sewage. That might justify more studies addressing the efficacy of NLV removal by sewage water treatment methods. NLV RNA was found by reverse transcriptase PCR (RT-PCR) in sewage water concentrates. Analysis of sequences of the RT-PCR products showed identical sequences in stools of patients and related sewage samples. In 6 of 11 samples not related to outbreaks, multiple NLV genotypes were seen.
- Published
- 1999