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DOI: 10.2514/1.20588 A human-in-the-loop simulation experiment was conducted at the NASA Langley Research Center's Air Traffic Operations Laboratory in an effort to comprehensively validate tools and procedures intended to enable the small aircraft transportation system higher-volume-operations concept of operations. These procedures were developed with the goal of increasing the rate of operations at nontowered, nonradar airports in near all-weather conditions. A key element of the new design is the establishment of a volume of airspace around designated airports at which pilots accept responsibility for self-separation. Flights operating at these airports are given approach-sequencing information computed by a ground-based automated system. The validation experiment was conducted to determine if a pilot could safely and proficiently fly an airplane while performing the newly developed procedures. Comparative measures of flight path error, perceived workload, and situation awareness were obtained for two types of simulated scenarios. Baseline scenarios were representative of today's system using procedure separation, in which air traffic control grants one approach or departure clearance at a time. Test scenarios represented the newly developed approach and departure procedures. Results from the experiment indicate that low-time pilots were able to fly the test procedures in a simulation environment and maintain self-separation as safely and proficiently as flying today's procedures.

DOI: 10.2514/1.20586 A multilayer approach to the prevention of conflicts due to the loss of aircraft-to-aircraft separation that relies on procedures and onboard automation was implemented as part of the Small Aircraft Transportation Systems Higher-Volume-Operations concept. The multilayer system gives pilots support and guidance during the execution of normal operations and advance warning for procedure deviations or off-nominal operations. This paper describes the major concept elements of this multilayer approach to separation assurance and conflict prevention and provides the rationale for its design. All the algorithms and functionality described in this paper were implemented in an aircraft simulation in the Air Traffic Operations Laboratory at NASA Langley Research Center and on the NASA Cirrus SR22 research aircraft.

One objective of the Small Aircraft Transportation System (SATS) Project is to increase the capacity and utilization of small non-towered, non-radar equipped airports by transferring traffic management activities to an automated system and separation responsibilities to general aviation (GA) pilots. This paper describes the development of a research multi-function display (MED) to support the interaction between pilots and an automated Airport Management Module (AMM). Preliminary results of simulation and flight tests indicate that adding the responsibility of monitoring other traffic for self-separation does not increase pilots' subjective workload levels. Pilots preferred using the enhanced MFD to execute flight procedures, reporting improved situation awareness (SA) over conventional instrument flight rules (IFR) procedures. Keywords: Small Aircraft Transportation System; SATS Higher Volume Operations; MFD and SATS; Data link and general aviation; Data link messaging

This paper summarizes findings from the Small Aircraft Transportation System Higher Volume Operations Flight Experiment. The higher volume operations concept improves efficiency at nontowered, nonradar airports in instrument meteorological conditions. The success of the higher volume operations concept is based on pilot acceptability as determined through objective and subjective assessments when compared with the procedural control operations in use today at nontowered, nonradar-controlled airfields in instrument meteorological conditions. Flight experiment data indicate that the concept is viable. The experiment, flown on a general aviation aircraft, used a subset of the Higher Volume Operations Simulation Experiment scenarios and evaluation pilots to validate the simulation experiment results. Results reveal that all 12 low-time instrument-rated pilots preferred Small Aircraft Transportation System Higher Volume Operations when compared with current procedural separation operations. These pilots also flew the higher volume operations procedures safely and proficiently without additional workload in comparison to today's system. Detailed results of pilot flight technical error and their subjective assessments of workload and situation awareness are presented. DOI: 10.2514/1.20403

The complementarity of site-directed mutagenesis and synthetic substrate analogues are used to investigate the dissociative mechanistic model for protein tyrosine kinase-catalyzed reaction and use of substrate neutral phenol in phosphorylation reaction, instead of chemically more reactive phenoxide anion.

Protein kinase chips, in which kinases are tested for their ability to phosphorylate immobilized substrates, have been developed and used to evaluate the protein kinases encoded by the yeast genome. This new technology promises to be a valuable addition to the biochemists' and cell biologists' arsenal for evaluating the substrate selectivity and function of protein kinases in cell signaling.