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Butyl benzyl phthalate (BBP), a typical phthalate plasticizer, is frequently detected in aquatic environments, but its possible effects on fish liver are unknown. In this study, adult zebrafish were exposed to 5-500 μg/L BBP and cultured for 28 days. The toxicity mechanism of environmentally relevant concentrations of BBP in the liver was explored using integrated biomarker response (IBR), molecular docking, and histopathological analysis, based on the tests of oxidative stress, apoptosis, and tissue damage, respectively. The results revealed that exposure to 500 μg/L BBP caused lipid peroxidation and DNA damage and induced inflammatory responses in the liver and intestinal tissues. The accumulation of reactive oxygen species (ROS) is the primary manifestation of BBP toxicity and is accompanied by changes in the activities of antioxidant and detoxification enzymes. Notably, the pro-apoptotic genes (p53 and caspase-3) were still significantly upregulated in the 50 μg/L and 500 μg/L treatment groups on day 28. Moreover, BBP interfered with apoptosis by forming a stable complex with apoptosis proteins (P53 and Caspase-3). Our findings are helpful for understanding the toxicity mechanisms of BBP, which could further promote the assessment of the potential environmental risks of BBP., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Phthalate esters (PAEs) are widely used as plasticizers and can be ubiquitously detected in environment. However, the toxic effects and mechanisms of diisononyl phthalate (DINP) on earthworms are still poorly understood. In this study, earthworms (Eisenia fetida) were exposed to DINP at various doses (0, 300, 600, 1200, and 2400 mg/kg) to investigate their subchronic toxicity. The results demonstrated that the reactive oxygen species (ROS) levels displayed an "increase-decrease" trend with the increasing DINP doses after DINP exposure on days 7, 14, 21, and 28. The malondialdehyde (MDA) content increased with increasing DINP doses on days 7, 14, and then decreased on days 21, 28. The values of superoxide dismutase (SOD), catalase (CAT), and glutathione S-transferase (GST) showed similar variation patterns and reached a maximum level on 21 d. Moreover, on day 28, the SOD and CAT gene expression levels were upregulated, while the GST gene expression levels were downregulated. Meanwhile, 16S rRNA genes of E. fetida gut bacteria and surrounding soil bacteria were measured after 28 days of exposure to DINP. The Chao index of E. fetida gut bacteria decreased when the treatment with the highest concentration (2400 mg/kg) was applied. At the phylum level, the abundance of Chloroflexi was significantly lower in the gut of E. fetida. In addition, the abundance of Proteobacteria at the phylum level and Ottowia at the genus level significantly increased in the surrounding soil. Overall, our results shed light on the toxic mechanism of DINP at biochemical, molecular, and omics levels, and contributed to a better understanding of the ecotoxicity of DINP., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2022 Elsevier B.V. All rights reserved.)

Metal contamination has become an increasingly severe environmental issue due to intense anthropogenic activities in recent decades. Many studies have reported a rapidly increasing trend of heavy metal contents in sedimentary records. In this study, two lacustrine sediment cores (LDL and YL) far away from scientific research stations were collected in Ny-Ålesund and analyzed for the vertical distributions of 17 elemental concentrations (Cu, Zn, Pb, Co, Ni, Cr, Sr, Ba, Mn, P, Ti, K 2 O, Na 2 O, CaO, MgO, Fe 2 O 3 , Al 2 O 3 ), CIA and TOC contents. The results indicated that only the proxies Pb, P, CaO, TOC, and CIA showed an increasing trend in the upper 7 cm section of the sediment cores, while most of the elements' concentrations decreased towards the surface. The rapid increase of TOC contents is likely related to the climate warming over the past 200 years, which promotes the prosperity of vegetation and thus leads to more input of organic matter into the lakes. Moreover, a large number of seabirds live around the sampling position and the seabird guano contains high concentrations of P, which could be regarded as an important nutrient source for vegetation. Additionally, the rapid climate warming could accelerate the chemical weathering rates, and thus lead to increased CaO contents in the sediment profiles according to its geological background. Therefore, the concentrations of other elements are very likely diluted by the high contents of organic matter and CaO in the upper part of the sediment cores. It is noteworthy that the rapidly increasing trend of Pb contents are related to the gas-oil powered generators in Ny-Ålesund and long-range atmospheric transport from Europe. This study highlighted the nonnegligible influence of climate warming on the inorganic elemental geochemistry distributions in remote lakes., Competing Interests: Declaration of competing interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Fildes Peninsula, with a high density of scientific stations, has been significantly impacted by anthropogenic activities. However, the contamination from penguins, a biovector that transports pollutants from ocean to land, has seldom been assessed. In this study, 32 lacustrine surface sediment samples on Fildes Peninsula and 8 lacustrine surface sediment samples on Ardley Island were collected to determine Cu, Zn, Pb, Ni, Cr, Cd, Co, Sb, Hg and P levels. The results showed that the heavy metal contents of lacustrine sediments on Ardley Island are significantly higher than those on Fildes Peninsula. The contaminants on Fildes Peninsula are mainly derived from anthropogenic activities, while the contaminants on Ardley Island are transported to the lacustrine sediments in the form of penguin guanos after a series of biomagnification in the food chain. The results indicated that the impact of penguin-transported contamination on Antarctic environment outweighs human activities near scientific stations in some areas. Therefore, more attention should be paid to the impacts of Antarctic animals on the Antarctic environment., (Copyright © 2018 Elsevier B.V. All rights reserved.)