1. Evaluation of syringin's neuroprotective effect in a model of neonatal hypoxic-ischemic brain injury.
- Author
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Ergon EY, Çelik A, Diniz G, Çolak R, Özdemir SA, Çalkavur Ş, and Yilmaz O
- Subjects
- Animals, Rats, Phenylpropionates pharmacology, Phenylpropionates therapeutic use, Glucosides pharmacology, Glucosides therapeutic use, Apoptosis drug effects, Interleukin-1beta metabolism, Neuroprotective Agents pharmacology, Hypoxia-Ischemia, Brain pathology, Hypoxia-Ischemia, Brain drug therapy, Rats, Wistar, Disease Models, Animal, Animals, Newborn
- Abstract
Background/aim: A significant cause of mortality and morbidity in the neonatal era is hypoxic-ischemic encephalopathy (HIE). This study examined the histopathological analysis and neuroprotective impact of syringin (SYR) in an experimental HIE rat model., Material and Methods: On the 7th postnatal day, 24 Wistar albino rats were evaluated in 3 groups using the HIE model under gas anesthesia. In the experiment, Group A received 10 mg/kg SYR plus dimethyl sulfoxide (DMSO), Group B received DMSO only, and Group C served as a sham group. Immunohistochemical techniques were used to assess apoptotic cell measurement and proinflammatory cytokines (TNF-α and IL-1β primary antibodies)., Results: Rats suffering from hypoxic-ischemic brain damage had their apoptosis assessed. The SYR and sham groups had statistically fewer cells undergoing apoptosis (p < 0.001). There was no difference between the groups in terms of IL-1β and TNF-α during immunohistochemical staining. Neuronal degeneration was significantly lower in the histological evaluation of the hippocampus in the SYR group (p = 0.01). A statistically significant difference (p = 0.01) was observed between the SYR and the control groups regarding pericellular and perivascular edema., Conclusion: SYR reduced apoptosis, perivascular and pericellular edema, and neuronal degeneration in rat cerebral tissue. These results raise the possibility that SYR may have a neuroprotective effect on the harm brought on by HIE. This is the first investigation of SYR's function within the HIE paradigm., Competing Interests: DisclaimerConflict of interest: None of the authors have a conflict of interest., (© TÜBİTAK.)
- Published
- 2023
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