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Lignocellulosic materials are the favoured feedstock for biorefineries due to their abundant availability and non-completion with food. Biobased technologies for refining these materials are limited mainly by the cost of biomass hydrolyzing enzymes, typically sourced from filamentous fungi. Therefore, considerable efforts have been directed at improving the quantity and quality of secreted lignocellulose degrading enzymes from fungi in order to attain overall economic viability. Process improvements and media engineering probably have reached their thresholds and further production enhancements require modifying the fungal metabolism to improve production and secretion of these enzymes. This review focusses on the types and mechanisms of action of known fungal biomass degrading enzymes, our current understanding of the genetic control exerted on their expression, and possible routes for intervention, especially on modulating catabolite repression, transcriptional regulators, signal transduction, secretion pathways etc., in order to improve enzyme productivity, activity and stability., (Copyright © 2021 Elsevier Ltd. All rights reserved.)

The indiscriminate use of pesticides leads to serious food safety and toxicity issues and threatens the environment and biodiversity. Pseudomonas nitroreducens AR-3 isolated from pesticide contaminated agricultural soil removed 97% of chlorpyrifos (CP) in just 8 h, in a mineral salt medium (MSM) containing glucose (1.0 g/L) and yeast extract (0.5 g/L) at 30 °C and 2% (v/v) inoculum when challenged with 100 mg/L CP. 3, 5, 6-trichloro 2-pyridinol (TCP), the degradation product of CP was detected only in low levels, indicating its further degradation. Organophosphate hydrolase (OPH), the enzyme considered responsible for CP degradation, had an intracellular localization. Crude OPH (1 mg/ml) removed 42% of 100 mg/L chlorpyrifos in just 2 h, indicating a rapid rate of degradation. Ultra-fast degradation of chlorpyrifos with an inducible OPH marks the potential of P. nitroreducens AR-3 for bioremediation of organophosphates. The strain AR-3 has the fastest rate of organophosphate degradation reported till date among Pseudomonads., (Copyright © 2019 Elsevier Ltd. All rights reserved.)